- FILM-Users - imperial.ac.uk

[FILM-Users 01164] ONI super resolution microscope seminar and demo in SAF!
by Gaboriau, David C A 17 Jul '24

17 Jul '24

Dear users, Please see the message below about a talk on the ONI super-resolution microscope and an upcoming demo. All the best, David ---------------------------------------------------- David Gaboriau, PhD (Cantab), FRMS Microscopy Specialist Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 408 +44 (0)20 7594 9793 d.gaboriau(a)imperial.ac.uk https://www.imperial.ac.uk/people/d.gaboriau http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ https://www.imperial.ac.uk/Stories/light-fantastic/ ---------------------------------------------------- ________________________________ From: Kelwick, Richard J R <r.kelwick(a)imperial.ac.uk> Sent: 17 July 2024 17:27 To: structural-and-synthetic-biology <structural-and-synthetic-biology(a)imperial.ac.uk> Subject: ONI super resolution microscope seminar and demo in SAF! Dear all, We are hosting a demo of the ONI Nanoimager, a benchtop super-resolution microscope. Applications include: cellular imaging/phenotyping, extracellular vesicles and more. See https://oni.bio/nanoimager/ Please contact Pip Timmins phillipa(a)oni.bio<mailto:phillipa@oni.bio> at ONI if you have any questions about the microscope. The demo will be based in SAF level 2 Lab Room 245 starting Monday 22nd July and there will also be a seminar and introduction to the system on Tuesday the 23rd of July 10am-11:30am in SAF lecture room G34. See attached flyer!!! If you would like to test your own samples on the microscope please fill in the form so that ONI can organise the demo and advise on sample prep. Samples will need approval before the demo so please fill in the form as soon as possible. Demo form: Here<https://docs.google.com/forms/d/e/1FAIpQLSeihJfGw-l3-HgtPSd_vDQl5fuJ5ZUWmlN…> Thanks, Richard Dr Richard J. R. Kelwick Senior Research Associate & RSE Enterprise Fellow Alumni Imperial College London Department of Infectious Disease Section of Structural and Synthetic Biology Sir Alexander Fleming Building South Kensington Campus Exhibition Road, London, SW7 2AZ, UK Member of Imperial Postdocs and Fellows Enterprise Network (IPFEN<https://www.imperial.ac.uk/postdoc-fellows-development-centre/networks-/ipf…>) Committee [signature_1912115680]@rkelwick<https://twitter.com/rkelwick> [signature_2296782183] http://www.linkedin.com/in/richardkelwick Google Scholar<https://scholar.google.co.uk/citations?user=rFzQd_4AAAAJ&hl=en> The contents of this E-mail are confidential.

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[FILM-Users 01163] Important! OBJECTIVE CLEANING
by Tisi, Madeline S 10 Jul '24

10 Jul '24

Hi all, We have received numerous complaints about oil objectives being left in poor conditions. You should all have been shown how to clean an objective and if you have not please ask FILM staff, who will go through the procedure below with you: 1. use a low-lint lens tissue produced especially for cleaning optics, or a cotton swab - all FILM microscopes have the materials required adjacent to the microscopes. 2. if using lens tissue, fold the lens tissue to form a stiff corner, and then add 70% ethanol to the area 3. clean the objective from the centre outwards in a spiral motion, if you have put too much oil on and you have drenched the entire objective please ensure you clean this us as it is not fair to the next user. The amount of oil required should not exceed more than double the size of the objective aperture. 4. Dispose of the lens tissue/swab and repeat until all oil residue is removed. [Steps for cleaning objective lenses - use a spiral motion, starting from the rim of the lens and moving to the centre.] Unfortunately, if the objectives keep being left in these conditions we will begin charging cleaning fees. Cleaning the objectives should be part of the shut down procedure: 1. close software/shut down as you have been instructed 2. remove sample 3. clean objective 4. escape objective to lower z limit 5. change to lowest magnification objective 6. ensure condenser is enclosed and you have not left any mess All in all, this takes minutes so please be considerate! Best wishes Maddy Maddy Tisi Advanced Microscopy Specialist Facility of Light Imaging Microscopy (FILM) National Heart and Lung Institute ____________________________________ Imperial College London ICTEM Building The Hammersmith Hospital, Du Cane Road, London W12 0NN FILM Website: https://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscop… LinkedIn: www.linkedin.com/in/madeline-tisi<http://www.linkedin.com/in/madeline-tisi> [cid:42a79790-9e74-42f0-a6de-c8a08e28b220]

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[FILM-Users 01162] Important Reminder: Proper Handling of Stage Inserts
by Nechyporuk-Zloy, Volodymyr 08 Jul '24

08 Jul '24

Dear Colleagues and FILM Users, We always consider the support and service to such a diverse and broad community of scientists as a great privilege and opportunity for us to grow in all aspects. We hope that FILM staff can support you to the best of our capability and that you can obtain excellent results here. This all requires mutual respect and understanding. In my previous email, I kindly asked our community to avoid damaging the stage inserts, as they cost more than £1,100 each, and we must cover these expenses from our own budget. Unfortunately, we have recently experienced another instance of damage to the stage insert dedicated to well-plate imaging. As you can see from the attached picture, there is substantial damage to this mechanical-optical element: [cid:e3d30cc5-5c1c-46ff-97f7-22f279970692] Optics and microscopy do not require forceful handling. Please avoid such actions and respect our equipment and work. Your cooperation is essential in maintaining the quality and availability of our resources for all users. Thank you for your understanding and support. Warm regards, Volodymyr Dr. Volodymyr Nechyporuk-Zloy (FILM Facility Manager) Facility for Imaging by Light Microscopy Imperial College of Science, Technology and Medicine Sir Alexander Fleming Building South Kensington Campus London SW7 2AZ, UK Mobile: (+44) 7526275617 Twitter: @vnzloy LinkedIn: https://www.linkedin.com/in/volodymyr-nechyporuk-zloy/ Company Web: https://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscop…

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[FILM-Users 01161] WF1 move to ICTEM delayed until October 2024
by Gaboriau, David C A 08 Jul '24

08 Jul '24

Dear users, Some of you wrote to use asking to keep WF1 in SAF building, and as the move from NHLI to Hammersmith is slightly delayed, we will keep WF1 in SAF until October 2024 at least. All the best, David ---------------------------------------------------- David Gaboriau, PhD (Cantab), FRMS Microscopy Specialist Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 408 +44 (0)20 7594 9793 d.gaboriau(a)imperial.ac.uk https://www.imperial.ac.uk/people/d.gaboriau http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ https://www.imperial.ac.uk/Stories/light-fantastic/ ----------------------------------------------------

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[FILM-Users 01160] Leica Applications Workshop Series: LASX Navigator – Thursday 18th July
by Nechyporuk-Zloy, Volodymyr 08 Jul '24

08 Jul '24

Leica Applications Workshop Series – Thursday 18th July Topic: LASX Navigator Overview: LAS X Navigator is a powerful tool for sample navigation and image acquisition that allows users to generate a fast overview image and identify important specimen details instantly. Like a GPS for samples, it provides a clear path to high-quality data, enabling users to navigate through samples, create fast overviews, identify details instantly, and run high-resolution image acquisition using templates for slides, dishes, and multi-well plates. This workshop offers an excellent opportunity for attendees to bring their own samples and receive support for their specific applications. Program: 10:30 – 11:00: Navigator Overview – Teams-based Webinar (Link provided upon registration) Workshop Sessions – Leica STELLARIS 8 and Navigator Operation: - Session One: 13:00-14:00 - Session Two: 14:15-15:15 - Session Three: 15:30-16:30 All Sessions are identical Location: Bessemer Building B118, South Kensington To register for a workshop session, please send details of the applications you are working with and your Lab/Group affiliation to: emmanuelle.steib(a)leica-microsystems.com.

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[FILM-Users 01159] WF1 moving to ICTEM on Hammersmith campus
by Gaboriau, David C A 27 Jun '24

27 Jun '24

Dear users, As part of NHLI's move to Hammersmith campus, the Zeiss widefield WF1 will move to ICTEM building on the Hammersmith campus on Tuesday 16th July. Once it is re-installed there, it will be available as previously in SAF. Apologies for the inconvenience, All the best, David ---------------------------------------------------- David Gaboriau, PhD (Cantab), FRMS Microscopy Specialist Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 408 +44 (0)20 7594 9793 d.gaboriau(a)imperial.ac.uk https://www.imperial.ac.uk/people/d.gaboriau http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ https://www.imperial.ac.uk/Stories/light-fantastic/ ----------------------------------------------------

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[FILM-Users 01158] Centre for Neurotechnology seminar 2 July - Jerry Chen: Imaging the Mammalian Brain Across Space and Time with Multi-Foci Multi-Photon Microscopy
by Nechyporuk-Zloy, Volodymyr 20 Jun '24

20 Jun '24

Dear all, Please join us on Tuesday 2nd July at 12pm for a seminar from Jerry Chen, Associate Professor of Biology at Boston University, on: Imaging the Mammalian Brain Across Space and Time with Multi-Foci Multi-Photon Microscopy<https://www.imperial.ac.uk/events/178847/imaging-the-mammalian-brain-with-m…> The seminar will be in-person in Seminar Room 120, Sir Alexander Fleming Building, South Kensington Campus. No registration is required. Follow the link above for full details and to add the event to your calendar. Best wishes, Amanda Foust — Amanda Foust, PhD Imperial College London Dept Bioengineering Centre for Neurotechnology Optical Neurophysiology Laboratory<https://opticalneurophysiology.org/> Lab: +44 (0)207 594 7026 Office: +44 (0)207 594 1055 a.foust(a)imperial.ac.uk<mailto:a.foust@imperial.ac.uk>

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[FILM-Users 01157] Microscopy course at the Crick
by Gaboriau, David C A 17 Jun '24

17 Jun '24

Dear users, There are still a few places available for the microscopy course at the Crick in September, this will be an excellent opportunity to improve skills in multi-dimensional imaging and image analysis Details below: https://www.crick.ac.uk/whats-on/crick-microscopy-course-multi-dimensional-… All the best, David ---------------------------------------------------- David Gaboriau, PhD (Cantab), FRMS Microscopy Specialist Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 408 +44 (0)20 7594 9793 d.gaboriau(a)imperial.ac.uk https://www.imperial.ac.uk/people/d.gaboriau http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ https://www.imperial.ac.uk/Stories/light-fantastic/ ----------------------------------------------------

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[FILM-Users 01156] Reminder: Proper Use of the Microscopy Equipment
by Nechyporuk-Zloy, Volodymyr 13 Jun '24

13 Jun '24

Dear Colleagues, We have noticed an issue with oil contamination on the objectives of our systems, which hinders normal usage. The FILM staff is currently addressing this by cleaning the affected objectives using stereo microscopes and established procedures. Please note that this cleaning process may take between 1-2 hours to complete thoroughly. Each morning, I endeavor to check the systems; however, the distance between our buildings makes it challenging to simultaneously monitor the microscopes in both the SAF and ICTEM buildings. This situation underscores the importance of each user's responsibility to maintain and respect our shared equipment. To further enhance our workflow and ensure compliance, we will implement a charge for the cleaning services in cases where users leave objectives contaminated. This policy will be enforced going forward to prevent such issues. Thank you for your attention to this matter and for your cooperation in maintaining the standards of our facility. Best regards, Volodymyr Dr. Volodymyr Nechyporuk-Zloy (FILM Facility Manager) Facility for Imaging by Light Microscopy Imperial College of Science, Technology and Medicine Sir Alexander Fleming Building South Kensington Campus London SW7 2AZ, UK Mobile: (+44) 7526275617 Twitter: @vnzloy LinkedIn: https://www.linkedin.com/in/volodymyr-nechyporuk-zloy/ Company Web: https://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscop…

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[FILM-Users 01155] Invitation to ZEISS Lightsheet 7 Imaging System Demonstration
by Nechyporuk-Zloy, Volodymyr 12 Jun '24

12 Jun '24

Dear Colleagues, In FILM, we are continually working to expand our imaging technological portfolio. >From the 27th of June to the 3rd of July, we will be hosting the ZEISS Lightsheet 7 imaging system<https://www.zeiss.com/microscopy/en/products/light-microscopes/light-sheet-…> for demonstration purposes in the SAF Building. A small Sample Preparation Guide is available here<https://www.youtube.com/watch?v=7LcrIZpcx0o>. We have several 3-hour slots available for demonstrations. If you are interested in participating, please let me know so we can allocate a time slot for you. Best regards, Volodymyr Dr. Volodymyr Nechyporuk-Zloy (FILM Facility Manager) Facility for Imaging by Light Microscopy Imperial College of Science, Technology and Medicine Sir Alexander Fleming Building South Kensington Campus London SW7 2AZ, UK Mobile: (+44) 7526275617 Twitter: @vnzloy LinkedIn: https://www.linkedin.com/in/volodymyr-nechyporuk-zloy/ Company Web: https://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscop…

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