- FILM-Users - imperial.ac.uk

[FILM-Users 01205] Request for a Leica vibratome
by Gaboriau, David C A 27 May '25

27 May '25

Dear users, Régis Joulia from NHLI is looking for a Leica vibratome, please see below: ------------------ Hello David, How are you doing in SK ? Is the building quieter now ? We are looking for a Leica Vibratome (VT1000/VT1200) for experiments requiring lymph nodes slices. Could you please send a message to the FILM mailing list to see if someone could help ? Thanks in advance, Régis --------------------- If you have one or know someone who does, please reply to Régis directly: r.joulia(a)imperial.ac.uk All the best, David ---------------------------------------------------- David Gaboriau, PhD (Cantab), FRMS Microscopy Specialist - Facility for Imaging by Light Microscopy (FILM) Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk - FILM area +44 (0)20 7594 9793 d.gaboriau(a)imperial.ac.uk https://www.imperial.ac.uk/people/d.gaboriau http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ https://www.imperial.ac.uk/Stories/light-fantastic/ ---------------------------------------------------- ________________________________ From: Joulia, Régis <r.joulia(a)imperial.ac.uk> Sent: 27 May 2025 12:40 To: Gaboriau, David C A <d.gaboriau(a)imperial.ac.uk> Subject: Leica vibratome Hello David, How are you doing in SK ? Is the building quieter now ? We are looking for a Leica Vibratome (VT1000/VT1200) for experiments requiring lymph nodes slices. Could you please send a message to the FILM mailing list to see if someone could help ? Thanks in advance, Régis

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[FILM-Users 01203] FILM Update & Upcoming Seminar Invitation
by Nechyporuk-Zloy, Volodymyr 01 May '25

01 May '25

Dear Colleagues, I’m writing to share a brief update on the current developments within FILM, as well as to highlight an upcoming seminar. First, I am very pleased to welcome Dilip Shrestha<https://www.linkedin.com/in/smilesdil/> to our team. Dilip brings with him many years of experience in advanced microscopy, including fluorescence correlation spectroscopy (FCS) and super-resolution (nanoscopy) techniques such as STED and STORM. At FILM, his focus will be on the development of tissue imaging and multiplex imaging capabilities. He will also be supporting the Leica CellDive<https://www.leica-microsystems.com/products/light-microscopes/p/cell-dive/> Imaging system and epifluorescence systems based at the Hammersmith Campus. In addition, I’d like to encourage you to attend the next event<https://www.eventbrite.co.uk/e/membrane-receptor-network-seminar-series-tic…> in the Membrane Receptor Network Seminar Series, hosted by Alejandra Tomas<https://profiles.imperial.ac.uk/a.tomas-catala/about>. We are delighted to welcome Professor Stefan W. Hell<https://nanobiophotonics.mpibpc.mpg.de/>, Nobel Laureate in Chemistry (2014)<https://www.nobelprize.org/prizes/chemistry/2014/hell/facts/>, who will be giving a lecture titled: “Molecule-scale resolution and dynamics in fluorescence microscopy” Date: Wednesday, 14th May Time: Arrivals from 2:30pm for a prompt 3:00pm start Venue: Lecture Theatre 2, Wolfson Education Centre, Hammersmith Hospital Campus, London This promises to be a truly inspiring session and an excellent opportunity to engage with one of the leading figures in the field of microscopy. With best wishes, Volodymyr Dr. Volodymyr Nechyporuk-Zloy (FILM Facility Manager) Facility for Imaging by Light Microscopy Imperial College of Science, Technology and Medicine Sir Alexander Fleming Building South Kensington Campus London SW7 2AZ UK OR ICTEM Building Du Cane Road 72 London W12 0NN UK Mobile: (+44) 7526275617 Twitter: @vnzloy LinkedIn: https://www.linkedin.com/in/volodymyr-nechyporuk-zloy/ Web: https://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscop…

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[FILM-Users 01202] FILM support and scheduling policy
by Nechyporuk-Zloy, Volodymyr 08 Apr '25

08 Apr '25

Dear Colleagues, We’re truly grateful for the broad interest and continued engagement of the imaging community with FILM — both in terms of training and instrumental support. It’s a privilege to work with such a dedicated and enthusiastic user base. At FILM, our staff have always strived to offer the highest level of support. Over the past two years, this has often meant going well beyond what’s expected: I personally left 26 days of annual leave unused in 2023, and 14 days in 2024. During long periods with only 50% staffing, we’ve routinely worked 50–60 hour weeks to ensure continuity and excellence. However, in 2025, including this April, we’ve come to a clear and important decision: in order to safeguard the sustainability of our service and the wellbeing of our staff, all team members are now strictly adhering to their official job descriptions and contracted hours. To make the best use of our time — across instrumental support, user assistance, and new technology development — we need your help. A few important requests: * Please avoid cancelling training sessions unless absolutely necessary. Each session takes significant time to organise, and we receive 2–4 training requests per day. Trainings are carefully adapted to individual needs and continuously improved through user feedback. * Plan your instrument bookings with care. Last-minute cancellations are challenging to reallocate. * Updated cancellation policy: To improve efficiency and fairness, we are increasing the cancellation notice period for both training and instrument bookings from 12 hours to 24 hours. We appreciate your understanding and cooperation as we continue to evolve FILM’s services in a sustainable, user-responsive direction. Thank you for working with us to keep this community thriving. Warm regards, Volodymyr Dr. Volodymyr Nechyporuk-Zloy (FILM Facility Manager) Facility for Imaging by Light Microscopy Imperial College of Science, Technology and Medicine Sir Alexander Fleming Building South Kensington Campus London SW7 2AZ UK OR ICTEM Building Du Cane Road 72 London W12 0NN UK Mobile: (+44) 7526275617 Twitter: @vnzloy LinkedIn: https://www.linkedin.com/in/volodymyr-nechyporuk-zloy/ Web: https://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscop…

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[FILM-Users 01201] Upcoming Seminar: Molecule-scale resolution and dynamics in fluorescence microscopy
by Nechyporuk-Zloy, Volodymyr 04 Apr '25

04 Apr '25

Dear Colleagues, We are delighted to invite you to an upcoming seminar hosted by Alejandra Tomas<https://profiles.imperial.ac.uk/a.tomas-catala> as part of the Membrane Receptor Network Seminar Series. We are honoured to welcome Prof. Stefan W. Hell, Nobel Laureate in Chemistry (2014), who will be presenting a talk entitled: “Molecule-scale resolution and dynamics in fluorescence microscopy” Date: Wednesday 14th May Time: Arrivals from 2:30pm for a prompt 3:00pm start Location: Lecture Theatre 2, Wolfson Education Centre, Hammersmith Hospital Campus, London, UK Please register your attendance using the following link<https://www.eventbrite.co.uk/e/membrane-receptor-network-seminar-series-tic…>. The abstract for the talk is attached for your convenience. We very much look forward to seeing many of you there for what promises to be a fascinating and insightful afternoon. Warm regards, Volodymyr

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[FILM-Users 01200] Fw: Demystifying HPC: Abstract Deadline extended!
by Gaboriau, David C A 31 Mar '25

31 Mar '25

Dear users, If you are using High Performance Computing (HPC) for your image analysis, or if you would like to know more about this, representatives of some of London universities are organising a one-day event at The Crick on 28th April. It aims to connect wet lab scientists and HPC teams, and is free to attend. Please see below to register and to submit an abstract for a 10min talk. All the best, David ---------------------------------------------------- David Gaboriau, PhD (Cantab), FRMS Microscopy Specialist - Facility for Imaging by Light Microscopy (FILM) Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk - FILM area +44 (0)20 7594 9793 d.gaboriau(a)imperial.ac.uk https://www.imperial.ac.uk/people/d.gaboriau http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ https://www.imperial.ac.uk/Stories/light-fantastic/ ---------------------------------------------------- ________________________________ From: The Francis Crick Institute <noreply(a)event.eventbrite.com> Sent: 31 March 2025 09:51 To: Gaboriau, David C A <d.gaboriau(a)imperial.ac.uk> Subject: Demystifying HPC: Abstract Deadline extended! This email from noreply(a)event.eventbrite.com originates from outside Imperial. Do not click on links and attachments unless you recognise the sender. If you trust the sender, add them to your safe senders list<https://spam.ic.ac.uk/SpamConsole/Senders.aspx> to disable email stamping for this address. [Eventbrite] This was sent to you by The Francis Crick Institute Thank you for signing up for our workshop, where we will be bringing together wet lab scientists, HPC teams and image analysts to work on a common language for all, and come up with a curriculum to help teach HPC use to beginner HPC users from the wet lab. On that note! We need some wet lab scientists to give a ~10 minute talk on their research, and how they would benefit from using HPC in their image analysis, or research. Or, if you are already using HPC in your work, how you're using it. Please consider submitting an abstract on what you'd like to talk about. Your voice here will help shape how we teach HPC to all the future users! Please send an abstract to todd.fallesen(a)crick.ac.uk,<mailto:todd.fallesen@crick.ac.uk,> by this Friday if you'd like to talk. The voice of the students is very important! Thanks! The coordination team Demystifying HPC: Unlocking High-Performance Computing for Researchers <https://clicks.eventbrite.com/f/a/P4sMC76HDJeH5mjPd79vGA~~/AAQxARA~/xVkJvC_…> Monday, April 28, 2025 from 8:30 AM to 5:30 PM (BST) The Francis Crick Institute 1 Midland Road London NW1 1AT United Kingdom View Order <https://clicks.eventbrite.com/f/a/zvslff_Pb00E8vpWKEmbhw~~/AAQxARA~/-F9bIgu…> Organized by The Francis Crick Institute<https://clicks.eventbrite.com/f/a/EH4Lbq2paexLLj8bn1RdAg~~/AAQxARA~/Cdzrt4_…> Questions about the event? Contact the organizer<https://clicks.eventbrite.com/f/a/K2tdMifEnVkPlBKiLh7o1g~~/AAQxARA~/n40Upjc…> This email was sent to d.gaboriau(a)imperial.ac.uk<mailto:d.gaboriau%40imperial.ac.uk> Sent using Eventbrite Copyright © 2025 Eventbrite. All rights reserved. Privacy Policy<https://clicks.eventbrite.com/f/a/Kebd9pyjswciDa6OgHX2bA~~/AAQxARA~/0a01hEf…>

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[FILM-Users 01199] Improving Access to FILM Imaging Equipment – New Cancellation Policy
by Nechyporuk-Zloy, Volodymyr 31 Mar '25

31 Mar '25

Dear Colleagues, At FILM, we strive to offer an innovative, effective, and efficient imaging service for the entire Imperial College London community. It is both a responsibility and a privilege to support such a diverse and pioneering group of researchers across departments. Our service depends greatly on your collaboration and engagement. With that in mind, we would like to address an ongoing challenge that affects the availability and fair use of our imaging systems: a high rate of booking cancellations. So far in 2024, 47% of sessions booked on our systems have been cancelled. Unfortunately, most of these cancellations occur too late to allow other users to take advantage of the freed-up time. As a result, microscopy time is lost and the efficiency of our shared resources suffers. To help address this, we are introducing a 12-hour cancellation deadline. From now on, bookings must be cancelled at least 12 hours in advance. We hope this change encourages more careful planning and helps all users make better use of available imaging time. We’re grateful for your continued support and look forward to improving the service together. Warm regards, Volodymyr Dr. Volodymyr Nechyporuk-Zloy (FILM Facility Manager) Facility for Imaging by Light Microscopy Imperial College of Science, Technology and Medicine Sir Alexander Fleming Building South Kensington Campus London SW7 2AZ UK OR ICTEM Building Du Cane Road 72 London W12 0NN UK Mobile: (+44) 7526275617 Twitter: @vnzloy LinkedIn: https://www.linkedin.com/in/volodymyr-nechyporuk-zloy/ Web: https://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscop…

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[FILM-Users 01198] Invitation: MINFLUX 3D Nanoscope Demonstration, 12–16 May
by Nechyporuk-Zloy, Volodymyr 18 Mar '25

18 Mar '25

Dear Colleagues, We invite you to a demonstration of the MINFLUX 3D Nanoscope<https://abberior.rocks/superresolution-confocal-systems/minflux/>, hosted by DMDR (Alejandra Tomas<https://profiles.imperial.ac.uk/a.tomas-catala>) and FILM at the ICTEM Building from 12th to 16th May. Developed by Nobel Laureate Stefan Hell and his team, the MINFLUX 3D Nanoscope offers isotropic localization precision below 3 nm, setting new benchmarks in fluorescence microscopy. This provides researchers with a powerful tool for addressing a range of biomedical and biophysical questions at the molecular scale. Further details can be found on our event homepage<https://www.imperial.ac.uk/events/191572/demonstration-of-the-minflux-3d-na…>. If you are interested in attending, please contact Alejandra Tomas and me. Best regards, Volodymyr

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[FILM-Users 01197] Welcome to the FILM Team – Razieh
by Nechyporuk-Zloy, Volodymyr 12 Mar '25

12 Mar '25

Dear Colleagues, I am delighted to introduce our newest member of the FILM team, Razieh<https://www.linkedin.com/in/razieh-khoshnevisan-ph-d-2926a01b4/> (r.khoshnevisan(a)imperial.ac.uk) Razieh brings a diverse background, starting in Veterinary Medicine before specialising in immunology and microscopy. She also has valuable commercial experience in the pharmaceutical drug discovery sector. At FILM, Razieh will be supporting our users with advanced microscopy at the Hammersmith Campus. Please join me in welcoming her—we wish her every success and an enjoyable time with us. Best regards, Volodymyr

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[FILM-Users 01196] Writing the method section for your imaging experiments
by Gaboriau, David C A 10 Mar '25

10 Mar '25

Dear users, I thought I would share some guidelines about writing microscopy methods for publications that we published last week with an international group of microscopists called QUAREP-LiMi. These guidelines are minimal to ensure proper reporting of imaging conditions, and we are working with journal publishers so that they will be required when submitting a publication. There are examples in the paper so that you can see what it looks like. I hope this is useful, please let me know if you have any question. All the best, David Link to publication: https://zenodo.org/records/14977578 ---------------------------------------------------- David Gaboriau, PhD (Cantab), FRMS Microscopy Specialist - Facility for Imaging by Light Microscopy (FILM) Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk - FILM area +44 (0)20 7594 9793 d.gaboriau(a)imperial.ac.uk https://www.imperial.ac.uk/people/d.gaboriau http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ https://www.imperial.ac.uk/Stories/light-fantastic/ ----------------------------------------------------

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[FILM-Users 01195] Fw: Invitation to Leica THUNDER Imager Workshop
by Nechyporuk-Zloy, Volodymyr 21 Feb '25

21 Feb '25

Dear Colleagues, We are excited to invite you to our upcoming workshop on the Leica THUNDER Imager Cell Spinning Disk—a cutting-edge solution for high-speed, high-resolution imaging with exceptional background suppression. The hands-on session will give you: • Insight into the system’s capabilities for live-cell imaging. • Tips and tricks for optimal performance in your imaging experiments • A chance to see how the THUNDER Imager can elevate your research Don’t miss this opportunity to sharpen your microscopy skills and network with peers in the imaging community. See below for an example image! Please register below and forward to all those you think may be interested. https://forms.office.com/e/0HB4KC7MSt We look forward to seeing you at the workshop! [cid:d6609112-44b0-493e-9021-29aeb653ecc0] [cid:d8cf3d46-01aa-436e-a0c4-9cb728d4248f] BW, Sharad

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