- FILM-Users - imperial.ac.uk

[FILM-Users 01134] Access to Scratch2 is restored
by Gaboriau, David C A 11 Dec '23

11 Dec '23

Dear users, The access to Scratch2 has been restored. Please could I emphasise that this drive is not intended for storage, only for transferring your data on the day of the session. The problem last week was possibly caused by the amount of data on the drive, so I will be deleting as needed. Please ensure you have appropriate back-ups of your data. All the best, David ---------------------------------------------------- David Gaboriau, PhD, FRMS Microscopy Specialist Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 408 +44 (0)20 7594 9793 d.gaboriau(a)imperial.ac.uk https://www.imperial.ac.uk/people/d.gaboriau http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ ----------------------------------------------------

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[FILM-Users 01133] Access to Scratch2 is down
by Gaboriau, David C A 07 Dec '23

07 Dec '23

Dear users, The access to Scratch2 is currently down, we are working with ICT to restore it. Apologies for the inconvenience, All the best, David ---------------------------------------------------- David Gaboriau, PhD, FRMS Microscopy Specialist Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 408 +44 (0)20 7594 9793 d.gaboriau(a)imperial.ac.uk https://www.imperial.ac.uk/people/d.gaboriau http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ ----------------------------------------------------

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[FILM-Users 01132] Introducing FILM's New Image Analysis and AI Support Initiative
by Nechyporuk-Zloy, Volodymyr 27 Nov '23

27 Nov '23

Dear Colleagues, I want to extend my thanks for your patience and understanding during the challenging times we are experiencing at the FILM imaging facility. Currently, we are facing a 50% staff shortage, and our dedicated team members are working tirelessly, often without annual leave and beyond normal working hours, to maintain the level of service you have come to expect from us. We are committed to modernizing our facility and transforming it into an innovative center for the growth of imaging and digital technologies. I would like to take a moment to reflect on the invaluable contributions of Steve, a fantastic imaging specialist who has significantly impacted the Imperial College Community. His dedication and expertise have rightfully earned him Awards for Outstanding Research Support, especially in the realm of image analysis. Steve's remarkable efforts in writing approximately 70 ImageJ/FIJI macros per year, each varying in complexity and provided free of charge outside working hours, have been a boon to our community. His work is well-documented<https://www.imperial.ac.uk/media/imperial-college/medicine/facilities/film/…> and can be accessed here<https://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscop…>. While it is challenging for FILM to replicate this level of service, we are determined to move forward and continue our progress. To this end, we are introducing a new system for those interested in image analysis or AI image research. We encourage you to utilize our new project request form<https://ppms.eu/imperial/req/?pf=2&project=true&form=179> for any related needs. Your requests will be processed based on time availability. FILM's image analysis and AI service will be supported by mature scientists, including David Gaboriau<https://www.imperial.ac.uk/people/d.gaboriau>, Anthony Sinadinos<https://www.imperial.ac.uk/people/a.sinadinos>, and Connor Darling<https://www.linkedin.com/in/connor-darling-b70856ab/?originalSubdomain=uk>. Furthermore, we are investing considerable effort into developing a robust IT and computational infrastructure. Our vision is for FILM to become a hub where users interested in IA and AI can discover new ideas, approaches, and connect with fantastic colleagues. Thank you once again for your continued support and understanding. Best regards, Volodymyr

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[FILM-Users 01131] Invitation to Zeiss Lattice Lightsheet 7 Demonstration
by Nechyporuk-Zloy, Volodymyr 09 Nov '23

09 Nov '23

Dear Colleagues, We are excited to extend an invitation to you for an upcoming demonstration<https://www.imperial.ac.uk/events/169247/discovering-the-subcellular-dynami…> of the new Zeiss Lattice Lightsheet 7. This event promises to offer valuable insights into the latest advancements in live cell imaging technology. The demonstration is scheduled for December 4-8, 2023, and will be held in room 527 of the Sir Alexander Fleming Building. This event is coordinated by Wesley Washington<https://www.linkedin.com/in/wesley-washington-9bb83896/>, Zeiss Account Manager, in collaboration with the FILM staff. Here's a brief abstract of what the demonstration will cover: Live imaging is crucial for biological research, offering insights that are among the most significant in the field. The challenges of achieving the necessary resolution in four dimensions at the speed required, while preserving cell physiology, are substantial. The ZEISS Lattice Lightsheet 7, a technology innovated by Nobel laureate Eric Betzig, is designed to overcome these challenges. It provides exceptionally gentle imaging and rapid acquisition speeds of up to three volumes per second. This expands the possibilities of live cell imaging considerably, from observing fast interactions of organelles in 3D to conducting extended time-lapses of photosensitive samples or processes. During the demonstration, we will delve into the technology and showcase the range of applications it enables. For more detailed information, please visit the following links: - ZEISS Lattice Lightsheet 7<https://www.zeiss.com/microscopy/en/products/light-microscopes/light-sheet-…> - Applications of Lattice Lightsheet Microscopy in Life Science Research <https://www.zeiss.com/microscopy/en/applications/life-sciences/lattice-ligh…> Should you be interested in attending the demonstration, please respond to this email, and we will ensure you are registered. We look forward to exploring this cutting-edge technology with you. Warm regards, Volodymyr Dr. Volodymyr Nechyporuk-Zloy (FILM Facility Manager) Facility for Imaging by Light Microscopy Imperial College of Science, Technology and Medicine Sir Alexander Fleming Building South Kensington Campus London SW7 2AZ, UK Mobile: (+44) 7526275617 Twitter: @vnzloy LinkedIn: https://www.linkedin.com/in/volodymyr-nechyporuk-zloy/ Company Web: https://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscop…

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[FILM-Users 01130] Update and Availability: HCF2 Microscope Detectors Replaced by Leica Team
by Nechyporuk-Zloy, Volodymyr 06 Nov '23

06 Nov '23

Dear Colleagues, I hope this message finds you well. I am pleased to inform you that the Leica Team has successfully replaced two detectors on our HCF2 microscope; the microscope is now fully operational and available for use. Should you encounter any issues or irregularities while utilizing the microscope, please do not hesitate to bring them to my attention - your feedback is invaluable to ensure the equipment functions smoothly for everyone's benefit. Thank you for your cooperation and understanding. Warm regards, Volodymyr

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[FILM-Users 01129] Post doctoral position with Dan Davis - super resolution imaging
by Gaboriau, David C A 02 Nov '23

02 Nov '23

Dear users, Please see below for an exciting post doc position with Dan Davis at Imperial, to study fundamental aspects of molecular recognition by human Natural Killer cells (and T cells), using super-resolution fluorescence microscopy as well as molecular and cell biology techniques. https://www.jobs.ac.uk/job/DDO837/research-associate-in-immune-cell-biology… All the best, David ---------------------------------------------------- David Gaboriau, PhD, FRMS Microscopy Specialist Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 408 +44 (0)20 7594 9793 d.gaboriau(a)imperial.ac.uk https://www.imperial.ac.uk/people/d.gaboriau http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ ----------------------------------------------------

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[FILM-Users 01128] PPMS now working again
by Gaboriau, David C A 01 Nov '23

01 Nov '23

Dear users, PPMS is now working again, so you are able to book microscopy sessions. Apologies for the inconvenience this morning. All the best, David ---------------------------------------------------- David Gaboriau, PhD, FRMS Microscopy Specialist Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 408 +44 (0)20 7594 9793 d.gaboriau(a)imperial.ac.uk https://www.imperial.ac.uk/people/d.gaboriau http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ ----------------------------------------------------

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[FILM-Users 01127] Temporary Issues with PPMS Instrument Booking
by Nechyporuk-Zloy, Volodymyr 01 Nov '23

01 Nov '23

Dear Users, We hope this message finds you well. We regret to inform you that we are currently experiencing some temporary issues with the PPMS booking for our instruments. Please rest assured that our team is working diligently to resolve this matter as swiftly as possible. We understand the inconvenience this may cause and appreciate your patience and understanding during this time. Should you have any questions or concerns, please do not hesitate to reach out. Thank you for your continued support. Warm regards, Volodymyr Dr. Volodymyr Nechyporuk-Zloy (FILM Facility Manager) Facility for Imaging by Light Microscopy Imperial College of Science, Technology and Medicine Sir Alexander Fleming Building South Kensington Campus London SW7 2AZ, UK Mobile: (+44) 7526275617 Twitter: @vnzloy LinkedIn: https://www.linkedin.com/in/volodymyr-nechyporuk-zloy/ Company Web: https://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscop…

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[FILM-Users 01126] Update on FILM: Staffing, New Initiatives, and Opportunities
by Nechyporuk-Zloy, Volodymyr 25 Oct '23

25 Oct '23

Dear Colleagues, I hope this message finds you well. I am writing to update you on the current status and future initiatives of the FILM imaging family. Staffing Levels For the past several months, David and I have been operating at 50% of our normal staffing levels. Despite this, we have been working 50-60 hours per week to meet your imaging needs. We apologize for any delays you may have experienced in training or consultation and appreciate your understanding as we work through these challenging times. To alleviate the workload, we are actively recruiting new staff and hope to return to a normal operating schedule in the near future. Call for Support We would greatly appreciate your support in these times. If you have more than one year of experience in advanced imaging or image analysis coding and would like to further your skills in user training and support, we invite you to join us as paid Casual Workers. Instrumental Portfolio & Future Plans Currently, FILM serves an extensive community—approximately 460 users across 132 scientific groups—with diverse imaging requirements. We are committed to meeting these varied needs to the best of our abilities. Over the past 7-8 months, we have conducted 4-5 demos and equipment tests to ensure that we are offering cutting-edge technologies. Based on a recent PI survey, we have identified top priorities for future technological development. One exciting update is the forthcoming installation of multiplex imaging capabilities. Additionally, we look forward to expanding our facilities with three new rooms in the ICTEM building come February, where some equipment from the SAF building will be relocated. Special Announcement: Super-Resolution Microscopy We are pleased to announce that we are promoting the use of super-resolution microscopy. As part of this initiative, we are introducing a flexible charging policy and reducing the rate for the Zeiss ELYRA microscope to £25/hour. We have also made significant advances in STED imaging techniques, achieving resolutions as fine as 60 nm and 90 nm depending on the depletion wavelength used. Attached you will find SIM images obtained by David and STED images obtained by the Leica team and myself. We thank you for your continued support and look forward to your active participation in enhancing the FILM community. Best regards, Volodymyr Dr. Volodymyr Nechyporuk-Zloy (FILM Facility Manager) Facility for Imaging by Light Microscopy Imperial College of Science, Technology and Medicine Sir Alexander Fleming Building South Kensington Campus London SW7 2AZ, UK Mobile: (+44) 7526275617 Twitter: @vnzloy LinkedIn: https://www.linkedin.com/in/volodymyr-nechyporuk-zloy/ Company Web: https://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscop…

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[FILM-Users 01125] Issues with HCF2 Microscope and Call for Feedback on HCF3 Image Quality
by Nechyporuk-Zloy, Volodymyr 23 Oct '23

23 Oct '23

Dear Colleagues, I hope this email finds you well. I would like to bring to your attention some important updates concerning our imaging facilities. Unfortunately, our HCF2 microscope is currently out of service due to a software issue affecting the hardware server. I have spent 5–6 hours trying to resolve the problem but without success. I have already reached out to the Leica Microsystems team for assistance, and they are currently working on diagnosing and fixing the issue. I will keep you updated on the progress, and I sincerely hope that the microscope will be back in operation as soon as possible. In addition to this, we have received 2–3 complaints stating that the HCF3 microscope does not produce the same quality of images as the HCF2 system. If you have experienced similar issues, we would greatly appreciate your feedback. Your input will be invaluable as we work to improve the performance of both systems. If you would like to contribute to this effort, please upload your comparative data to the following folder: scratch2\_Service\_Issues\HCF3\HCF2\_HCF3\_Comparison. Your cooperation in this matter will help us make necessary adjustments and ensure that both systems meet our standards of quality. Thank you for your understanding and cooperation. I apologize for any inconvenience this may have caused and assure you that we are doing our best to resolve these issues promptly. Best regards, Volodymyr Dr. Volodymyr Nechyporuk-Zloy (FILM Facility Manager) Facility for Imaging by Light Microscopy Imperial College of Science, Technology and Medicine Sir Alexander Fleming Building South Kensington Campus London SW7 2AZ, UK Mobile: (+44) 7526275617 Twitter: @vnzloy LinkedIn: https://www.linkedin.com/in/volodymyr-nechyporuk-zloy/ Company Web: https://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscop…

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