- FILM-Users - imperial.ac.uk

[FILM-Users 01154] Cooperation and Care in Using Imaging Facility Equipment
by Nechyporuk-Zloy, Volodymyr 10 Jun '24

10 Jun '24

Dear Colleagues, We are very thankful for your continued usage of our imaging facility and the unwavering support you provide to our staff. Our goal is to work with you in full unity and mutual understanding to maintain a productive and efficient environment. However, I would like to remind everyone of the importance of cooperation with our staff and careful handling of our equipment. Recently, I mentioned the need to be cautious with our stage inserts, as two of them were damaged in a similar manner. The total cost for replacement is £2,220, equivalent to the price of a good laptop. This expense will be covered by our facility budget, which strains our resources. Additionally, please exercise care with the objectives. Our staff consistently provides guidance on proper handling, and we ask that you follow these instructions diligently. Thank you in advance for your attention and cooperation. Best regards, Volodymyr Dr. Volodymyr Nechyporuk-Zloy (FILM Facility Manager) Facility for Imaging by Light Microscopy Imperial College of Science, Technology and Medicine Sir Alexander Fleming Building South Kensington Campus London SW7 2AZ, UK Mobile: (+44) 7526275617 Twitter: @vnzloy LinkedIn: https://www.linkedin.com/in/volodymyr-nechyporuk-zloy/ Company Web: https://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscop…

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[FILM-Users 01153] Notice of Limited FILM Staff Support (June 4-7)
by Nechyporuk-Zloy, Volodymyr 30 May '24

30 May '24

Dear Colleagues, We would like to inform you that from June 4th to 7th, there will be limited support from our FILM staff, as two of our team members will be attending the annual meeting of the European Light Microscopy Initiative<https://www.elmi2024.org/> in Liverpool. I will attempt to monitor and respond to emails during this period. Thank you for your understanding. Best regards, Volodymyr Dr. Volodymyr Nechyporuk-Zloy (FILM Facility Manager) Facility for Imaging by Light Microscopy Imperial College of Science, Technology and Medicine Sir Alexander Fleming Building South Kensington Campus London SW7 2AZ, UK Mobile: (+44) 7526275617 Twitter: @vnzloy LinkedIn: https://www.linkedin.com/in/volodymyr-nechyporuk-zloy/ Company Web: https://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscop…

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[FILM-Users 01152] Update on The Confocal System Usage for Summer: HCF1, HCF2, HCF3
by Nechyporuk-Zloy, Volodymyr 23 May '24

23 May '24

Dear Colleagues, I hope this message finds you well. To enhance access to the confocal systems in the ICTEM Building, viz. HCF1, HCF2, HCF3, during the summer months (May-August), we are making some adjustments to the usage policy. Effective immediately, we will remove the weekly usage limit while maintaining a daily limit of 6 hours. This change aims to improve accessibility and ensure fair usage among all users. Please note that these adjustments are provisional. Based on your feedback and any technical issues that may arise, we are open to revising this policy or reverting to the previous settings. Thank you for your cooperation and understanding. We look forward to your feedback to better tailor these arrangements to our community's needs. Best regards, Volodymyr Dr. Volodymyr Nechyporuk-Zloy (FILM Facility Manager) Facility for Imaging by Light Microscopy Imperial College of Science, Technology and Medicine Sir Alexander Fleming Building South Kensington Campus London SW7 2AZ, UK Mobile: (+44) 7526275617 Twitter: @vnzloy LinkedIn: https://www.linkedin.com/in/volodymyr-nechyporuk-zloy/ Company Web: https://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscop…

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[FILM-Users 01151] Leica workshop: TauSense Imaging:
by Nechyporuk-Zloy, Volodymyr 15 May '24

15 May '24

Dear Colleagues, I am pleased to inform you that on Thursday, 23rd May, Leica will host the first in a series of application-based workshops at the Leica Imaging Hub<https://www.imperial.ac.uk/bioengineering/about/facilities-and-services/bio…>, located in Bessemer Building B118, South Kensington. Topic – TauSense Imaging: TauSense provides dynamic access to Fluorescence Lifetime 'Functional Imaging' information in real time and in parallel with fluorescence intensity images. Utilize lifetime-based information to explore molecular functions within a cellular context, enhance image quality, and distinguish fluorescent species beyond spectral methods. Workshop Highlights: 1. Differentiate between desired and unwanted fluorescence signals, e.g., remove Autofluorescence or reflected light (TauGating, GateScan). 2. Separate fluorophores using lifetime-based information, e.g., visualize more fluorophores, eliminate cross-excitation (TauSeparation, TauScan). 3. Highlight regions of FRET activity or mean lifetime changes (Functional Imaging, Bio Sensors). 4. Directly visualize distinct structures based on lifetime differences (TauContrast), e.g., 1 detector imaging 2 or 3 lifetime-based channels – Multiplexing, Spatial Biology. Program: 9:30 – 10:00 AM: TauSense Overview – Teams-based Webinar (Link provided upon registration) Workshop Sessions on Leica STELLARIS 8 and TauSense Operation: Session One: 10:30 AM - 12:00 PM Session Two: 1:00 PM - 2:30 PM Session Three: 2:30 PM - 4:00 PM To reserve your spot, please contact Emmanuelle Steib at emmanuelle.steib(a)leica-microsystems.com. Best regards, Volodymyr

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[FILM-Users 01150] Scratch2 clean-up
by Gaboriau, David C A 26 Apr '24

26 Apr '24

Dear users, This is a general message to remind you that you should not leave data on Scratch2, as it is intended for transfer only, and not for storage. The drive is consistently getting full and failed before Christmas as it was too full. ICT managed to repair it, but if it happens again it might not be repairable, and all data would be lost. To ensure this doesn't happen, I am deleting data older than a month on a rolling basis, as your back-ups should be done on the day of the session, as instructed during training. Please make sure you have multiple back-ups of your data. All the best, David ---------------------------------------------------- David Gaboriau, PhD (Cantab), FRMS Microscopy Specialist Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 408 +44 (0)20 7594 9793 d.gaboriau(a)imperial.ac.uk https://www.imperial.ac.uk/people/d.gaboriau http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ https://www.imperial.ac.uk/Stories/light-fantastic/ ----------------------------------------------------

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[FILM-Users 01149] Invitation to Advanced Microscopy Applications Symposium at Imperial College London
by Nechyporuk-Zloy, Volodymyr 15 Apr '24

15 Apr '24

Dear FILM Users, I am pleased to invite you to the upcoming imaging symposium titled "Advanced Microscopy Applications" to be held on Wednesday, 17th April, from 11:00 AM to 4:15 PM. This event will showcase the sophisticated confocal and widefield microscopy systems at the Leica and Imperial Imaging Hub. Program Highlights - 11:00 - 11:30 AM - Arrival & Registration (Refreshments provided) - 11:30 - 11:35 AM - Welcome by Periklis (Laki) Pantazis, Imperial College London - 11:35 - 11:45 AM - Opening Address by Dario Farina, Imperial College London - 11:45 - 11:55 AM - Address from Leica by Darin Stell - 11:55 AM - 12:15 PM - Presentation by Dr. Annalisa Rizza on THUNDER Imagers and Mica - 12:15 - 12:40 PM - Mica applications discussed by Dr Virginia Silio, UCL - 12:40 - 1:00 PM - Insights on STELLARIS Confocal Platform by Dr Emmanuelle Steib - 1:00 - 1:30 PM - Talk by Marina Kuimova on molecular rotors - 1:30 - 2:15 PM - Lunch break - 2:15 - 4:15 PM - Microscopy showcases (Registration required) Please follow this link<https://calendly.com/leica_microsystems/imaging-symposium-at-imperial-colle…> to register and secure your place for this special event. A light buffet lunch and refreshments will be provided. Best regards, Volodymyr

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[FILM-Users 01148] Easter break - turn off all microscopes
by Gaboriau, David C A 27 Mar '24

27 Mar '24

Dear users, As FILM and College are closing tonight for Easter break, please could you make sure to turn off all microscopes and computers today, and also after each session if you come during the break You must have the appropriate permissions in place if you want to access the microscopes. In addition to normal lone worker access permissions (needed for weekend or evening access) you will also need College closure day access permission. If you don’t have this in place already you can not use the microscopes before FILM reopens on Wednesday 3rd April. We wish you a happy and healthy Easter break! All the best, David ---------------------------------------------------- David Gaboriau, PhD (Cantab), FRMS Microscopy Specialist Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 408 +44 (0)20 7594 9793 d.gaboriau(a)imperial.ac.uk https://www.imperial.ac.uk/people/d.gaboriau http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ https://www.imperial.ac.uk/Stories/light-fantastic/ ----------------------------------------------------

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[FILM-Users 01147] Microscopy Specialist position in Cambridge: CRUK-CI is hiring
by Gaboriau, David C A 27 Mar '24

27 Mar '24

Dear users, CRUK-Cambridge Institute is hiring a Microscopy Specialist, please see details attached. All the best, David ---------------------------------------------------- David Gaboriau, PhD (Cantab), FRMS Microscopy Specialist Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 408 +44 (0)20 7594 9793 d.gaboriau(a)imperial.ac.uk https://www.imperial.ac.uk/people/d.gaboriau http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ https://www.imperial.ac.uk/Stories/light-fantastic/ ----------------------------------------------------

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[FILM-Users 01145] Please remember to turn off microscopes at the end of the day
by Gaboriau, David C A 12 Mar '24

12 Mar '24

Dear users, Just a reminder to please check on PPMS at the end of your session to see if you need to turn off the microscope and computer: * if there is no one booked for the next 2h15 or more * if you are the last user of the day Many thanks and happy imaging! All the best, David ---------------------------------------------------- David Gaboriau, PhD (Cantab), FRMS Microscopy Specialist Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 408 +44 (0)20 7594 9793 d.gaboriau(a)imperial.ac.uk https://www.imperial.ac.uk/people/d.gaboriau http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ https://www.imperial.ac.uk/Stories/light-fantastic/ ----------------------------------------------------

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[FILM-Users 01146] Issues with HCF2 Confocal Microscope
by Nechyporuk-Zloy, Volodymyr 11 Mar '24

11 Mar '24

Dear Colleagues, I hope this email finds you well. I am reaching out to inform you that we are currently facing some challenges with our HCF2 confocal microscope, particularly with DIC imaging and the alignment of the confocal head and the stage. As a result, the microscope is not producing the quality of images we expect. We are actively working to resolve these issues as quickly as possible. I will keep you updated on our progress and any changes. Best regards, Volodymyr

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