- FILM-Users - imperial.ac.uk

[FILM-Users 01102] WF3 is working again
by Gaboriau, David C A 16 Jun '23

16 Jun '23

Dear users, We are very pleased to say that WF3 is back in working order after working with Zeiss to fix the computer issues. At the moment, please use the Zeiss account, not your Imperial College details, to log in, as the PC is not on the IC domain yet. The Zeiss account has no password. The Scratch2 drive should be mapped, but to access it you will need to log in. Please enter the following: 'IC\username' for username, and 'your IC password' for password Apologies for the inconvenience caused by this problem and thank you for your patience, All the best, David ---------------------------------------------------- David Gaboriau, PhD, FRMS Microscopy Specialist Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 408 +44 (0)20 7594 9793 d.gaboriau(a)imperial.ac.uk https://www.imperial.ac.uk/people/d.gaboriau http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ ----------------------------------------------------

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[FILM-Users 01101] FW: ANTI-FLAG antibody produced in rabbit
by Rothery, Stephen M 16 Jun '23

16 Jun '23

Dear All If anyone can help Liliane- see below - please contact her directly El Eid, Liliane l.eleid17(a)imperial.ac.uk<mailto:l.eleid17@imperial.ac.uk> DO NOT REPLY TO THIS E_MAIL Bw steve From: El Eid, Liliane <l.eleid17(a)imperial.ac.uk> Sent: 15 June 2023 16:34 To: Rothery, Stephen M <s.rothery(a)imperial.ac.uk> Subject: ANTI-FLAG antibody produced in rabbit Hi Steve, I am in need of a small aliquot of anti-flag antibody produced in rabbit to check for endogenous receptor expression in my mice. I have one produced in mice and am getting a lot of unspecific binding due to IgG. Could you please ask around? Best wishes Liliane Sent from Outlook for Android<https://aka.ms/AAb9ysg>

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[FILM-Users 01100] FW: Oligomycin
by Rothery, Stephen M 13 Jun '23

13 Jun '23

Dear All If anyone can help Amina - see below - please contact her directly Abulimiti, Amina a.abulimiti21(a)imperial.ac.uk<mailto:a.abulimiti21@imperial.ac.uk> DO NOT REPLY TO THIS E_MAIL Bw steve From: Abulimiti, Amina <a.abulimiti21(a)imperial.ac.uk> Sent: 13 June 2023 12:35 To: Rothery, Stephen M <s.rothery(a)imperial.ac.uk> Subject: Oligomycin Hi Steve, We are urgently looking for 10 uL of oligomycin (1-10mM). Would you mind circulating this email in the building to see if anyone has some to spare? Thank you very much in advance! All the best Amina - Amina Abulimiti PhD student Department of Brain Sciences Imperial College London Burlington Danes, Hammersmith Hospital London W12 0NN UK

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[FILM-Users 01099] FILM Facility Update: New Imaging Technology and Demo Report
by Nechyporuk-Zloy, Volodymyr 13 Jun '23

13 Jun '23

Dear Colleagues, We extend our heartfelt gratitude for choosing to collaborate with FILM - our imaging and microscopy facility. It is both a great honor and privilege to provide support to such a diverse and vibrant community. Our dedicated team strives to fulfill your demands and exceed your expectations at every opportunity. In our pursuit of advancing imaging technologies, we have had the unique privilege of being the first in the UK to test a cutting-edge NIKON super-resolution microscope. I am pleased to attach the comprehensive demo report prepared by the NIKON UK team, which highlights the features and capabilities of this advanced imaging technology. We express our deepest gratitude to all participants and groups involved in the demo for their invaluable contributions and for graciously sharing their imaging data. It is through your collaborative efforts that we can continue to expand our knowledge and deliver innovative solutions to our community. We remain dedicated to staying at the forefront of scientific advancements and ensuring that FILM remains a leading-edge facility for your research needs. As we explore new avenues in imaging, we encourage you to provide feedback and share your experiences with us. Your input plays a vital role in our ongoing pursuit of excellence and enables us to tailor our services to your specific requirements. Thank you once again for your ongoing trust and support. Wishing you a productive and inspiring day ahead. Best regards, Volodymyr

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[FILM-Users 01098] FILM: limited support on 31.05.2023
by Nechyporuk-Zloy, Volodymyr 30 May '23

30 May '23

Dear Colleagues, It is with regret that we communicate the unavailability of full on-site support for tomorrow, due to the impending industrial action within the rail system. In view of these circumstances, we will be providing limited online support during this period. We appreciate your understanding and cooperation during this time. Should you have any urgent matters that require immediate attention, please do not hesitate to reach out to us. Best regards, Volodymyr

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[FILM-Users 01097] !!!!!!!!!!!!!! DATA DELETION SCRATCH2 !!!!!!!!!!!!!!!!!!!!!!!
by Rothery, Stephen M 16 May '23

16 May '23

Dear All The scratch2 drive is getting full, so we need to delete older files from it We will delete files older than the 1st September 2022 next week NB This will include data files (eg exel data etc) Please make sure you have everything backed up from before then Also you are reminded that the scratch drive is for transferring FILM data only It is not for long term storage or for users to store other data and programs If you are working offsite then scratch can be accessed either through VPN or using the remote gateway to logon to you work PC Many thanks Steve FILM - Facility for Imaging by Light Microscopy ************************************************************** Steve Rothery FILM Technician Sir Alexander Fleming Building, desk 403 Imperial College London / South Kensington Exhibition Road London SW7 2AZ Tel +44-(0)207 594 9793 And ICTEM, (L-Block) Room 314 Hammersmith Campus Imperial College London Du Cane Road London, W12 0NN Tel. +44-(0)207 594 1669 E-mail s.rothery(a)imperial.ac.uk<mailto:s.rothery@imperial.ac.uk> Website: http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ **************************************************************

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[FILM-Users 01096] FILM: limited support on 12.05.2023
by Nechyporuk-Zloy, Volodymyr 11 May '23

11 May '23

Dear Colleagues, We regret to inform you that we will be providing only limited online support in light of the upcoming rail industrial action. https://www.nationalrail.co.uk/travel-information/industrial-action/ We apologize for any inconvenience this may cause. Best regards, Volodymyr

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[FILM-Users 01095] NIKON seminar: NSPARC - new super resolution detector
by Nechyporuk-Zloy, Volodymyr 09 May '23

09 May '23

Dear Colleagues, We would like to invite you to a NIKON seminar dedicated to the new super-resolution confocal microscopy (11.05.2023 at 14:00; SAF 113, Sir Alexander Fleming Building): https://www.imperial.ac.uk/events/162351/reveal-new-confocal-imaging-potent… Confocal laser scanning microscopy (CLSM) is an indispensable technology throughout the scientific community. By its principle, CLSM allows fluorophore emission detection (typically by a single-element detector like a photomultiplier tube) passing through a pinhole to reject out-of-focus light. By this means, the pinhole (usually set at 1AU) is primarily known for providing an optical section and therefore an improvement in the image signal-to-noise ratio (S/N). Closing the pinhole yields a resolution improvement but with a trade-off of heavy signal loss causing a reduction of the image S/N. To overcome the compromise between an increase in resolution and S/N, Nikon developed a new detector for AX igniting new confocal imaging potential. Based on the imaging scanning microscopy (ISM) technology, Nikon SPatial ARray Confocal detector (NSPARC) equipped with an array detector allows outstanding acquisition of super-resolved (SR) images equivalent to 0.2AU with S/N corresponding to 1AU. NSPARC detector can be a standalone detector or integrated with an additional confocal detector unit bringing extremely flexible hardware configuration. Combined with AX featuring the largest field of view in the market and gentle fast acquisition, NSPARC provides a simple acquisition, and unprecedented SR image quality, beyond confocal imaging. The seminar is a part of our NIKON demo: https://www.imperial.ac.uk/events/161521/nikon-confocal/ We still have a few available time slots in case you want to test the system and obtain more data. Thank you in advance for your attention, Volodymyr

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[FILM-Users 01094] Antibody request
by Rothery, Stephen M 02 May '23

02 May '23

Dear All Aanya Hirdaramani, PhD student with Prof Aylin Hanyaloglu at IRDB, is looking for a few ul of the following antibodies (all to react w human thank you): * anti-cleaved / activated notch * Hes1 * phospho and total Akt * IGF-1 * GLP-2 If you are able to spare some antibody, please contact her directly. Hirdaramani, Aanya aanya.hirdaramani15(a)imperial.ac.uk<mailto:aanya.hirdaramani15@imperial.ac.uk> Many thanks, DO NOT REPLY TO THIS E_MAIL Bw steve FILM - Facility for Imaging by Light Microscopy ************************************************************** Steve Rothery FILM Technician Sir Alexander Fleming Building, desk 403 Imperial College London / South Kensington Exhibition Road London SW7 2AZ Tel +44-(0)207 594 9793 And ICTEM, (L-Block) Room 314 Hammersmith Campus Imperial College London Du Cane Road London, W12 0NN Tel. +44-(0)207 594 1669 E-mail s.rothery(a)imperial.ac.uk<mailto:s.rothery@imperial.ac.uk> Website: http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ **************************************************************

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[FILM-Users 01093] Miltenyibiotec (Lavision) light-sheet demo and clearing of samples
by Nechyporuk-Zloy, Volodymyr 27 Apr '23

27 Apr '23

Dear Colleagues, To explore new opportunities and expand our imaging capabilities, we would like to organize a new demo for light-sheet imaging system from Miltenyibiotec (potentially, in June): https://www.miltenyibiotec.com/GB-en/products/ultramicroscope-blaze.html [cid:2f15d795-9e45-4453-aece-0019674d17da] The system has set of different objectives and option for high-throughput imaging of cleared samples of different sizes: [cid:3e3ec178-a723-4194-8377-f50767dd35d5] In addition to the scope, the company is also happy to provide the required reagents for the clearing procedures: [cid:aa2f6ad3-3dbe-44fc-b320-5337ff5ec183] https://www.miltenyibiotec.com/GB-en/products/macs-imaging-and-microscopy/l… and advise in your clearing. There is also a list of 3D validated antibodies. Furthermore, on demand, the team will/can also provide cleared samples which can be interesting for our users, e.g. bone, heart, lung, etc. Would you be so kind to inform me if you are interested to participate in the demonstration and/or obtain more information? Thank you in advance for your cooperation and support. Best regards, Volodymyr

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