- FILM-Users - imperial.ac.uk

[FILM-Users 01068] FW: WGA
by Rothery, Stephen M 09 Dec '22

09 Dec '22

Dear All If anyone can help Justin - see below, please contact him directly Zhang, Junqing junqing.zhang20(a)imperial.ac.uk<mailto:junqing.zhang20@imperial.ac.uk> DO NOT REPLY TO THIS E_MAIL Bw Steve From: Zhang, Junqing <junqing.zhang20(a)imperial.ac.uk> Sent: 08 December 2022 10:12 To: Rothery, Stephen M <s.rothery(a)imperial.ac.uk> Subject: WGA Hello Steve, I am looking for some Wheat germ agglutinin (WGA) lectin. Would you mind circulating this email in the building to see if anyone has some to spare? Thank you very much in advance! Best wishes, Justin

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[FILM-Users 01067] FW: Melanoma cell line
by Rothery, Stephen M 08 Dec '22

08 Dec '22

Dear All If anyone can help Ghita - see below, please contact her directly Ghislat, Ghita ghita.ghislat(a)imperial.ac.uk<mailto:ghita.ghislat@imperial.ac.uk> DO NOT REPLY TO THIS E_MAIL Bw Steve --------------------------------------------------------------------------- Dear Steve, I am looking for the following two cell lines: * YUMM1.7 Mouse Melanoma Cell Line * YUMMER1.7 Mouse Melanoma Cell Line Would you mind circulating this email through Imperial to see if anyone has some to spare? Many thanks in advance Best wishes Ghita

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[FILM-Users 01066] FW: Bcl-xl Antibody
by Rothery, Stephen M 07 Dec '22

07 Dec '22

Dear All If anyone can help Amina - see below, please contact her directly Abulimiti, Amina a.abulimiti21(a)imperial.ac.uk<mailto:a.abulimiti21@imperial.ac.uk> DO NOT REPLY TO THIS E_MAIL Bw Steve From: Abulimiti, Amina <a.abulimiti21(a)imperial.ac.uk> Sent: 06 December 2022 15:10 To: Rothery, Stephen M <s.rothery(a)imperial.ac.uk> Subject: Bcl-xl Antibody Hi Steve, I am looking for 5ul of Bcl-xl Antibody. Would you mind circulating this email in the building to see if anyone has some to spare? Thank you very much in advance! Best, Amina Sent from Outlook for iOS<https://aka.ms/o0ukef>

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[FILM-Users 01064] Access to scratch
by Rothery, Stephen M 06 Dec '22

06 Dec '22

Dear All there seems to be a problem connecting to the scratch drive at the moment ICT have been informed Bw steve FILM - Facility for Imaging by Light Microscopy ************************************************************** Steve Rothery FILM Technician Sir Alexander Fleming Building, desk 403 Imperial College London / South Kensington Exhibition Road London SW7 2AZ Tel +44-(0)207 594 9793 And ICTEM, (L-Block) Room 314 Hammersmith Campus Imperial College London Du Cane Road London, W12 0NN Tel. +44-(0)207 594 1669 E-mail s.rothery(a)imperial.ac.uk<mailto:s.rothery@imperial.ac.uk> Website: http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ **************************************************************

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[FILM-Users 01063] FW: TSG101 antibody for Extracellular Vesicles
by Rothery, Stephen M 22 Nov '22

22 Nov '22

Dear All If anyone can help Laura - see below, please contact her directly Nicastro, Laura l.nicastro19(a)imperial.ac.uk<mailto:l.nicastro19@imperial.ac.uk> DO NOT REPLY TO THIS E_MAIL Bw Steve From: Nicastro, Laura <l.nicastro19(a)imperial.ac.uk> Sent: 21 November 2022 14:57 To: Rothery, Stephen M <s.rothery(a)imperial.ac.uk> Subject: TSG101 antibody for Extracellular Vesicles Hi Steve, I need a small aliquot of the antibody TSG101 for extracellular vesicles detection. Would you mind circulating this email around to see if anyone has a small aliquot to spare? Thank you so much I really appreciate the help! Best wishes, Laura

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[FILM-Users 01062] Help request: Endometriotic cell lines
by Gaboriau, David C A 18 Nov '22

18 Nov '22

Dear users, If you can help Magdalene, please reply to her directly at magdalene.ho15(a)imperial.ac.uk Do not reply to this email If it's not too much trouble, I was wondering if you could help send out an email to the FILM community to ask if anyone has a spare vial of endometriotic cell lines we can use to try out before purchasing our own? For example, but not limited to: * Endometriotic epithelial cell line (eg. EEC12Z) * Endometriotic stromal cell line (eg. ESC22B, St-T1b, RL-7566) Many thanks! All the best, David ---------------------------------------------------- David Gaboriau, PhD, FRMS Interim FILM Facility Manager Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 408 +44 (0)20 7594 9793 d.gaboriau(a)imperial.ac.uk https://www.imperial.ac.uk/people/d.gaboriau http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ ---------------------------------------------------- ________________________________ From: Ho, Magdalene Y <magdalene.ho15(a)imperial.ac.uk> Sent: 17 November 2022 11:33 To: Gaboriau, David C A <d.gaboriau(a)imperial.ac.uk> Cc: Esanu, Ioana <ioana.esanu19(a)imperial.ac.uk> Subject: Endometriotic cell lines Dear David, Hope you are well! If it's not too much trouble, I was wondering if you could help send out an email to the FILM community to ask if anyone has a spare vial of endometriotic cell lines we can use to try out before purchasing our own? For example, but not limited to: * Endometriotic epithelial cell line (eg. EEC12Z) * Endometriotic stromal cell line (eg. ESC22B, St-T1b, RL-7566) Thank you, David! Best Regards, Mag

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[FILM-Users 01061] Floor cleaning level 3 ICTEM Saturday 26th
by Rothery, Stephen M 15 Nov '22

15 Nov '22

Dear All For those who use FILM microscopes at ICTEM, the lab area on level 3 will undergo annual floor polishing on Saturday 26th November 2022 9am - 5pm. There will be NO access to the lab area during the cleaning period, so you wont be able to use the microscopes in room 312/314 Apologies for any inconvenience Bw Steve FILM - Facility for Imaging by Light Microscopy ************************************************************** Steve Rothery FILM Technician Sir Alexander Fleming Building, desk 403 Imperial College London / South Kensington Exhibition Road London SW7 2AZ Tel +44-(0)207 594 9793 And ICTEM, (L-Block) Room 314 Hammersmith Campus Imperial College London Du Cane Road London, W12 0NN Tel. +44-(0)207 594 1669 E-mail s.rothery(a)imperial.ac.uk<mailto:s.rothery@imperial.ac.uk> Website: http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ **************************************************************

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[FILM-Users 01056] [Seminar] Cell DIVE Multiplexed Imaging | 15th November
by Gaboriau, David C A 15 Nov '22

15 Nov '22

Dear Users, I am pleased to welcome you to a FILM Club seminar on Tuesday 15th November, in person in SAF Building room 119 (ground floor) and also on Teams (see link below). Dr David Pointu, from Leica, will tell us about Cell DIVE, a Multiplexed Imaging system. Please see information below. Please contact me if you wish to attend. Looking forward to seeing you there, All the best, David ---------------------------------------------------- David Gaboriau, PhD, FRMS Interim FILM Facility Manager Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 408 +44 (0)20 7594 9793 d.gaboriau(a)imperial.ac.uk https://www.imperial.ac.uk/people/d.gaboriau http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ ---------------------------------------------------- ________________________________________________________________________________ Microsoft Teams meeting Join on your computer or mobile app Click here to join the meeting<https://teams.microsoft.com/l/meetup-join/19%3ameeting_ODBiODNiNTQtZmRmZi00…> Meeting ID: 372 501 980 254 Passcode: J7hDGz Download Teams<https://www.microsoft.com/en-us/microsoft-teams/download-app> | Join on the web<https://www.microsoft.com/microsoft-teams/join-a-meeting> [https://www.imperial.ac.uk/ict/assets/imperial_blue_small_logo.jpg] Learn more<https://aka.ms/JoinTeamsMeeting> | Meeting options<https://teams.microsoft.com/meetingOptions/?organizerId=113c5bee-3703-4299-…> | Legal<https://www.imperial.ac.uk/media/imperial-college/administration-and-suppor…> ________________________________________________________________________________ See what Cell DIVE can do for you No images? View in web browser<https://www2.leica-microsystems.com/webmail/149501/1128943961/ac89d18dd9840…>. [cid:image001.gif@01D8F46D.8E95BC60]<https://www2.leica-microsystems.com/e/149501/2022-11-03/6m1pnz/1128943961?h…> [cid:image002.png@01D8F46D.8E95BC60]<https://www2.leica-microsystems.com/e/149501/-multiplexed-imaging-glossary-…> Register for the upcoming seminar now! Dear Scientist, Join us to learn about Cell DIVE: An antibody-based hyperplexed imaging technique addressing spatial cell biology and function. In this one-hour hybrid seminar, Dr David Pointu, Senior Application Manager at Leica Microsystems, will share his expertise on this state-of-the-art technology. Cell DIVE features: * Reveal up to 60 biomarkers in one tissue sample * Over 350 commercially available antibodies have been characterized * High-quality image data, extremely accurate registration and stitching technology * Assess neighborhoods of each individual cell in the tissue microenvironment To join us in person or online, please contact Dr David Gaboriau<mailto:d.gaboriau@imperial.ac.uk>. WHEN: Tuesday 15ᵗʰ November 2022, 12:00-13:00 GMT WHERE: Room SAF 119 (ground floor) Sir Alexander Fleming Building Imperial College London South Kensington Campus Exhibition Road, London SW7 2AZ [cid:image003.png@01D8F46D.8E95BC60]<https://www2.leica-microsystems.com/e/149501/ns-to-south-kensington-campus-…> This event will also be streamed online. [cid:image004.png@01D8F46D.8E95BC60]<mailto:d.gaboriau@imperial.ac.uk> Kind regards Your Leica Microsystems Cell DIVE Team [Twitter]<https://www2.leica-microsystems.com/e/149501/leicamicro/6m1pp9/1128943961?h…> [Facebook]<https://www2.leica-microsystems.com/e/149501/LeicaMicrosystems-/6m1ppd/1128…> [Instagram]<https://www2.leica-microsystems.com/e/149501/leica-microsystems-/6m1pph/112…> [Youtube]<https://www2.leica-microsystems.com/e/149501/user-LeicaMicrosystems/6m1ppl/…> [LinkedIn]<https://www2.leica-microsystems.com/e/149501/company-leica-microsystems/6m1…> [cid:image010.png@01D8F46D.8E95BC60]<https://www2.leica-microsystems.com/e/149501/science-lab-/6m1pps/1128943961…> tell a friend<https://www2.leica-microsystems.com/e/149501/offer-addthis-unique-lng-en-us…> Our mailing address is: Leica Microsystems (UK) Ltd Larch House, Woodlands Business Park, Breckland, Linford Wood Milton Keynes, MK14 6FG United Kingdom Office Phone: 0800 298 2344 Fax: +44 (0)1908 577 640 Email: UKSales(a)leica-microsystems.com<mailto:UKSales@leica-microsystems.com> Copyright © 2022, All rights reserved. unsubscribe from this list<https://www2.leica-microsystems.com/unsubscribe/u/149501/ac89d18dd9840be9c8…> Confidential - Company Proprietary Please be advised that this email may contain confidential information. If you are not the intended recipient, please notify us by email by replying to the sender and delete this message. The sender disclaims that the content of this email constitutes an offer to enter into, or the acceptance of, any agreement; provided that the foregoing does not invalidate the binding effect of any digital or other electronic reproduction of a manual signature that is included in any attachment.

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[FILM-Users 01059] !!!!!!!!!!!!! DATA DELETION SCRATCH2 !!!!!!!!!!!!!!!!!!!!!!!
by Rothery, Stephen M 14 Nov '22

14 Nov '22

Dear All The scratch2 drive is getting full, so we need to delete older files from it We will delete files older than the 1st April 2022 next week NB This will include data files (eg exel data etc) Please make sure you have everything backed up from before then Also you are reminded that the scratch drive is for transferring FILM data only It is not for long term storage or for users to store other data and programs If you are working offsite then scratch can be accessed either through VPN or using the remote gateway to logon to you work PC Many thanks Steve FILM - Facility for Imaging by Light Microscopy ************************************************************** Steve Rothery FILM Technician Sir Alexander Fleming Building, desk 403 Imperial College London / South Kensington Exhibition Road London SW7 2AZ Tel +44-(0)207 594 9793 And ICTEM, (L-Block) Room 314 Hammersmith Campus Imperial College London Du Cane Road London, W12 0NN Tel. +44-(0)207 594 1669 E-mail s.rothery(a)imperial.ac.uk<mailto:s.rothery@imperial.ac.uk> Website: http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ **************************************************************

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[FILM-Users 01055] FW: Non-treated 6-well plate
by Rothery, Stephen M 27 Oct '22

27 Oct '22

Dear All If anyone can help Amina - see below, please contact her directly ABULIMITI, AMINA a.abulimiti21(a)imperial.ac.uk<mailto:a.abulimiti21@imperial.ac.uk> DO NOT REPLY TO THIS E_MAIL Bw Steve From: ABULIMITI, AMINA <a.abulimiti21(a)imperial.ac.uk> Sent: 27 October 2022 13:37 To: Rothery, Stephen M <s.rothery(a)imperial.ac.uk> Subject: Non-treated 6-well plate Hi Steve, I am looking for a non-treated 6-well plate. Would you mind circulating this email in the building to see if anyone has some to spare? Thank you very much in advance! Best, Amina - Amina Abulimiti PhD student Department of Brain Sciences Imperial College London Burlington Danes, Hammersmith Hospital London W12 0NN UK

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