- FILM-Users - imperial.ac.uk

[FILM-Users 01124] FILM: Hyper-plex microscopy to our community.
by Nechyporuk-Zloy, Volodymyr 20 Oct '23

20 Oct '23

Dear FILM users, We are excited to welcome the Leica Cell Dive<https://www.leica-microsystems.com/products/light-microscopes/p/cell-dive/> into FILM during the coming weeks, bringing hyper-plex microscopy to our community. So we can best arrange info sessions and trainings, could you please reply to me if you are interested in using this technology in the next 6-12 months. When you reply, please indicate in your email which group you are in (PI name) and what campus you are based at. Thank you, and happy imaging, Volodymyr Dr. Volodymyr Nechyporuk-Zloy (FILM Facility Manager) Facility for Imaging by Light Microscopy Imperial College of Science, Technology and Medicine Sir Alexander Fleming Building South Kensington Campus London SW7 2AZ, UK Mobile: (+44) 7526275617 Twitter: @vnzloy LinkedIn: https://www.linkedin.com/in/volodymyr-nechyporuk-zloy/ Company Web: https://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscop… Personal Homepage: microscopist.uk<http://microscopist.uk>

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[FILM-Users 01123] Help request: purified p53 protein sample
by Gaboriau, David C A 20 Oct '23

20 Oct '23

Dear users, If anyone is able to help Jacopo Gabrielli, he is looking for a sample of purified p53 protein as a Western blot control. Please reply directly to Jacopo at jg2721(a)ic.ac.uk Please do not reply to this email. Many thanks! All the best, David ---------------------------------------------------- David Gaboriau, PhD, FRMS Microscopy Specialist Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 408 +44 (0)20 7594 9793 d.gaboriau(a)imperial.ac.uk https://www.imperial.ac.uk/people/d.gaboriau http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ ----------------------------------------------------

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[FILM-Users 01122] Antibody request
by Gaboriau, David C A 06 Oct '23

06 Oct '23

Dear users, Please see below, if you can help Sam? Do not reply directly to this email, please contact Sam directly at s.reitemeier(a)imperial.ac.uk --------------- Hello everyone, Does anyone have access to any of these antibodies/stains? Anti-Cardiac Troponin I Antibody (ab47003) https://www.abcam.com/products/primary-antibodies/cardiac-troponin-i-antibo… Cell Signaling Technology Phopho-Troponin I (Cardiac) (Ser23/24) Antibody https://www.fishersci.com/shop/products/polyclonal-antibody-100-ul-pho-143/… Pro-Q™ Diamond Phosphoprotein Gel Stain https://www.thermofisher.com/order/catalog/product/P33300?gclid=CjwKCAjwvfm… Pro-Q™ Diamond Phosphoprotein Gel Destaining Solution https://www.fishersci.co.uk/shop/products/molecular-probes-pro-q-diamond-ph… Please feel free to email me at sreiteme(a)ic.ac.uk<mailto:sreiteme@ic.ac.uk> if you have any of these available. Thank you very much for your help. Warm regards, Sam ------------------ All the best, David ---------------------------------------------------- David Gaboriau, PhD, FRMS Microscopy Specialist Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 408 +44 (0)20 7594 9793 d.gaboriau(a)imperial.ac.uk https://www.imperial.ac.uk/people/d.gaboriau http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ ----------------------------------------------------

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[FILM-Users 01121] FILM's Work-from-Home Schedule on 4th and 6th October 2023 Due to Industrial Actions
by Nechyporuk-Zloy, Volodymyr 28 Sep '23

28 Sep '23

Dear Colleagues, I hope this message finds you well. I am writing to inform you that the FILM team will be working from home on the 4th and 6th of October 2023. This decision has been made in light of the planned industrial actions affecting public transportation. For more information on the industrial actions, you can visit the following websites: - National Rail Industrial Action Information: https://www.nationalrail.co.uk/travel-information/industrial-action/ - TFL Strikes Information: https://tfl.gov.uk/campaign/strikes We understand that this change in work location may cause some inconvenience to your scientific work, and we sincerely apologize for any disruptions this may cause. We will do our utmost to ensure that our work continues as smoothly as possible during these days. If you have any questions or concerns, please do not hesitate to reach out. Thank you for your understanding and cooperation. Best regards, Volodymyr Dr. Volodymyr Nechyporuk-Zloy (FILM Facility Manager) Facility for Imaging by Light Microscopy Imperial College of Science, Technology and Medicine Sir Alexander Fleming Building South Kensington Campus London SW7 2AZ, UK Mobile: (+44) 7526275617 Twitter: @vnzloy LinkedIn: https://www.linkedin.com/in/volodymyr-nechyporuk-zloy/ Company Web: https://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscop… Personal Homepage: microscopist.uk<http://microscopist.uk>

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[FILM-Users 01120] Fw: request for Nile Red
by Gaboriau, David C A 25 Sep '23

25 Sep '23

Dear users, Please see below, if you can help Alejandra who is looking for a drop of Nile Red ---------------------------- Dear David, I am looking for a small amount of Nile Red to check lipid droplet formation in our cells. I was wondering if you would be able to ask around FILM Users if we could borrow some from someone. We are in Hammersmith Campus. Thanks BW Alejandra --------------------------- Reply directly to a.tomas-catala(a)imperial.ac.uk Please do not reply to this email All the best, David ---------------------------------------------------- David Gaboriau, PhD, FRMS Microscopy Specialist Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 408 +44 (0)20 7594 9793 d.gaboriau(a)imperial.ac.uk https://www.imperial.ac.uk/people/d.gaboriau http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ ---------------------------------------------------- ________________________________ From: Tomas Catala, Alejandra D D <a.tomas-catala(a)imperial.ac.uk> Sent: 25 September 2023 15:51 To: Gaboriau, David C A <d.gaboriau(a)imperial.ac.uk> Cc: Austin, Greg <greg.austin21(a)imperial.ac.uk> Subject: Dear David, I am looking for a small amount of Nile Red to check lipid droplet formation in our cells. I was wondering if you would be able to ask around FILM Users if we could borrow some from someone. We are in Hammersmith Campus. Thanks BW Alejandra Sent from my iPhone

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[FILM-Users 01119] SAF analysis PC is out of use
by Gaboriau, David C A 15 Sep '23

15 Sep '23

Dear users, We're sorry to say that the SAF analysis PC is out of use, and needs to be rebuilt by ICT. They will do this as fast as they can, but we don't have a date yet. Apologies for the inconvenience, All the best, David ---------------------------------------------------- David Gaboriau, PhD, FRMS Microscopy Specialist Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 408 +44 (0)20 7594 9793 d.gaboriau(a)imperial.ac.uk https://www.imperial.ac.uk/people/d.gaboriau http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ ----------------------------------------------------

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[FILM-Users 01118] Important Notice: Staff Shortage and Exciting Opportunity
by Nechyporuk-Zloy, Volodymyr 15 Sep '23

15 Sep '23

Dear Users of the FILM Imaging Facility, I hope this message finds you well. We want to take a moment to express our gratitude for the privilege of serving such a vibrant and diverse community, consisting of 460 users from 130 groups across the entire Imperial College. Your imaging needs have been at the heart of our mission, and we are honored to support your work. Over the past few weeks, we have faced a significant challenge with a substantial shortage of staff, amounting to a 50% deficit in our team. We anticipate that this situation may persist for the next few months. We kindly ask for your understanding and patience during this time, as there may be possible delays in our response to your requests. We sincerely apologize for any inconvenience this may cause in the coming days. Additionally, we would like to draw your attention to an exciting opportunity within our facility. We are currently advertising for the position of Laboratory Manager and Senior Technician: https://www.imperial.ac.uk/jobs/description/MED03898/laboratory-manager-and… This is an ideal permanent role for an ambitious microscopist and image analysis specialist who is dedicated to user support and training. In this role, you will have the chance to enhance your knowledge in lab management while working with advanced imaging systems, including the tauSTED microscope (including FLIM) and various confocal microscopes. Furthermore, there are opportunities to expand your expertise in software development, particularly in ImageJ, Python, or Java programming, as there is a growing demand for ImageJ macros and plugins. Our facility fosters a dynamic and vibrant environment. Over the past 8-9 months, we have organized four complete testing and demo sessions of cutting-edge advanced imaging systems, ensuring that we stay at the forefront of imaging technology. We appreciate your understanding and support during this challenging period and encourage anyone interested in joining our team to explore this exciting opportunity. Thank you for being a part of our community, and we look forward to continuing to serve your imaging needs. Best regards, Volodymyr Dr. Volodymyr Nechyporuk-Zloy (FILM Facility Manager) Facility for Imaging by Light Microscopy Imperial College of Science, Technology and Medicine Sir Alexander Fleming Building South Kensington Campus London SW7 2AZ, UK Mobile: (+44) 7526275617 Twitter: @vnzloy LinkedIn: https://www.linkedin.com/in/volodymyr-nechyporuk-zloy/ Company Web: https://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscop…

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[FILM-Users 01116] !!!!!!!!!!!!!! DATA DELETION SCRATCH2 !!!!!!!!!!!!!!!!!!!!!!!
by Gaboriau, David C A 14 Sep '23

14 Sep '23

Dear Users, The scratch2 drive is getting full, so we need to delete older files from it. We will delete files older than the 15th June 2023 next week. NB This will include data files (eg exel data etc). Please make sure you have everything backed up from before then. Also you are reminded that the scratch drive is for transferring FILM data only. It is not for long term storage or for users to store other data and programs. If you are working offsite then Scratch2 can be accessed either through VPN or using the remote gateway to logon to you work PC. Many thanks All the best, David ---------------------------------------------------- David Gaboriau, PhD, FRMS Microscopy Specialist Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 408 +44 (0)20 7594 9793 d.gaboriau(a)imperial.ac.uk https://www.imperial.ac.uk/people/d.gaboriau http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ ---------------------------------------------------- ________________________________ From: Gaboriau, David C A <d.gaboriau(a)imperial.ac.uk> Sent: 14 September 2023 10:33 To: Gaboriau, David C A <d.gaboriau(a)imperial.ac.uk> Subject: Fw: [FILM-Users 01097] !!!!!!!!!!!!!! DATA DELETION SCRATCH2 !!!!!!!!!!!!!!!!!!!!!!! All the best, David ---------------------------------------------------- David Gaboriau, PhD, FRMS Microscopy Specialist Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 408 +44 (0)20 7594 9793 d.gaboriau(a)imperial.ac.uk https://www.imperial.ac.uk/people/d.gaboriau http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ ---------------------------------------------------- ________________________________ From: film-users-bounces(a)imperial.ac.uk <film-users-bounces(a)imperial.ac.uk> on behalf of Rothery, Stephen M <s.rothery(a)imperial.ac.uk> Sent: 16 May 2023 09:49 To: film-users <film-users(a)imperial.ac.uk> Subject: [FILM-Users 01097] !!!!!!!!!!!!!! DATA DELETION SCRATCH2 !!!!!!!!!!!!!!!!!!!!!!! Dear All The scratch2 drive is getting full, so we need to delete older files from it We will delete files older than the 1st September 2022 next week NB This will include data files (eg exel data etc) Please make sure you have everything backed up from before then Also you are reminded that the scratch drive is for transferring FILM data only It is not for long term storage or for users to store other data and programs If you are working offsite then scratch can be accessed either through VPN or using the remote gateway to logon to you work PC Many thanks Steve FILM - Facility for Imaging by Light Microscopy ************************************************************** Steve Rothery FILM Technician Sir Alexander Fleming Building, desk 403 Imperial College London / South Kensington Exhibition Road London SW7 2AZ Tel +44-(0)207 594 9793 And ICTEM, (L-Block) Room 314 Hammersmith Campus Imperial College London Du Cane Road London, W12 0NN Tel. +44-(0)207 594 1669 E-mail s.rothery(a)imperial.ac.uk<mailto:s.rothery@imperial.ac.uk> Website: http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ **************************************************************

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[FILM-Users 01115] Post-doc position in Cambridge
by Gaboriau, David C A 30 Aug '23

30 Aug '23

Dear users, Please see below for a post doctoral position in Cambridge, to work on cell/tissue level morphogenesis and relationship to lineage in the human lung. Especially suitable for a quantitative/mechanobiology/live imaging/imaging analysis background. https://www.jobs.cam.ac.uk/job/42060/ All the best, David ---------------------------------------------------- David Gaboriau, PhD, FRMS Microscopy Specialist Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 408 +44 (0)20 7594 9793 d.gaboriau(a)imperial.ac.uk https://www.imperial.ac.uk/people/d.gaboriau http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ ----------------------------------------------------

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[FILM-Users 01114] Steve Rothery - contact details
by Rothery, Stephen M 29 Aug '23

29 Aug '23

Dear All As most of you are probably aware, I retire on Thursday. If you wish to contact me after Thursday, then please use the following e-mail address as my college e-mail will no longer work. Stephen Rothery SR_analysis(a)outlook.com<mailto:SR_analysis@outlook.com> Finally, it has been a real pleasure working with you all and I wish you all the greatest of success. Best wishes. Steve FILM - Facility for Imaging by Light Microscopy ************************************************************** Steve Rothery FILM Technician E-mail s.rothery(a)imperial.ac.uk<mailto:s.rothery@imperial.ac.uk> Website: http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ **************************************************************

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