- FILM-Users - imperial.ac.uk

[FILM-Users 01079] FW: Ki67 Ab staining
by Rothery, Stephen M 28 Feb '23

28 Feb '23

Dear All If anyone can help Bavi - see below - please contact her directly Vijayakumar, Bavithra <bavithra.vijayakumar06(a)imperial.ac.uk<mailto:bavithra.vijayakumar06@imperial.ac.uk>> DO NOT REPLY TO THIS E_MAIL Bw steve From: Vijayakumar, Bavithra <bavithra.vijayakumar06(a)imperial.ac.uk<mailto:bavithra.vijayakumar06@imperial.ac.uk>> Sent: 27 February 2023 15:04 Subject: Ki67 Ab staining Hi Steve Hope you are well. I am trying to locate some Ki 67 Ab to look for cellular proliferation. We seem to have run out of this and I'm in urgent need of some more before the stock arrives. Is there any chance you could circulate this email please in case someone might have some. Best wishes Bavi

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[FILM-Users 01078] FW: donkey anti-rat secondary
by Rothery, Stephen M 22 Feb '23

22 Feb '23

Dear All If anyone can help Helen - see below - please contact her directly Stoelting, Helen helen.stoelting17(a)imperial.ac.uk<mailto:helen.stoelting17@imperial.ac.uk> DO NOT REPLY TO THIS E_MAIL Bw steve From: Stoelting, Helen <helen.stoelting17(a)imperial.ac.uk> Sent: 22 February 2023 14:05 To: Rothery, Stephen M <s.rothery(a)imperial.ac.uk> Subject: donkey anti-rat secondary Hi Steve I'm looking for a small aliquot (< 5 µl) of a fluorescent donkey anti-rat secondary in AF488 or AF647 to test a new stain. Would you be able to help by circulating this email? Thanks in advance for your help. Best wishes Helen Helen Stölting | she/her Research Assistant ë Inflammation, Repair and Development National Heart and Lung Institute Sir Alexander Fleming Building, floor 3, desk 24 Imperial College London, South Kensington Campus London, SW7 2AZ, United Kingdom * helen.stoelting17(a)imperial.ac.uk<mailto:helen.stoelting17@imperial.ac.uk> * Lab Website<https://lloydlab.co.uk/> | Imperial Profile<https://www.imperial.ac.uk/people/helen.stoelting17> [cid:image001.png@01D946C6.A1F72D60] @HelenIsInTheLab<https://twitter.com/HelenIsInTheLab> | @Lloyd_lab<https://twitter.com/lloyd_lab> My working hours may not be your working hours. Please do not feel obligated to reply outside your normal work schedule.

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[FILM-Users 01077] 73rd Annual Conference of the British Microcirculation and Vascular Biology Society to be held on June 29th-30th, 2023
by Rothery, Stephen M 14 Feb '23

14 Feb '23

73rd Annual Conference of the British Microcirculation and Vascular Biology Society to be held on June 29th-30th, 2023 See link below Dear all, Andrea Caporali (as member of the local organising committee) would like to advertise this symposium hosted by Edinburgh in June. Website:https://www.microcirculation.org.uk/ It is relevant for us vascular biologists, including for people developing interest on the brain vasculature (Axel Montagne is one of the group leaders of the Edinburgh Dementia Research Institute - DRI). Thank you and best wishes Costanza Professor Costanza Emanueli British Heart Foundation Chair of Cardiovascular Science National Heart & Lung Institute, Imperial College London FILM - Facility for Imaging by Light Microscopy ************************************************************** Steve Rothery FILM Technician Sir Alexander Fleming Building, desk 403 Imperial College London / South Kensington Exhibition Road London SW7 2AZ Tel +44-(0)207 594 9793 And ICTEM, (L-Block) Room 314 Hammersmith Campus Imperial College London Du Cane Road London, W12 0NN Tel. +44-(0)207 594 1669 E-mail s.rothery(a)imperial.ac.uk<mailto:s.rothery@imperial.ac.uk> Website: http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ **************************************************************

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[FILM-Users 01075] Cannot connect to the Scratch dive
by Rothery, Stephen M 13 Feb '23

13 Feb '23

Dear All Unfortunately it is not possible to connect to the scratch drive at the moment It has been reported to ICT and will hopefully be back working later today Bw Steve FILM - Facility for Imaging by Light Microscopy ************************************************************** Steve Rothery FILM Technician Sir Alexander Fleming Building, desk 403 Imperial College London / South Kensington Exhibition Road London SW7 2AZ Tel +44-(0)207 594 9793 And ICTEM, (L-Block) Room 314 Hammersmith Campus Imperial College London Du Cane Road London, W12 0NN Tel. +44-(0)207 594 1669 E-mail s.rothery(a)imperial.ac.uk<mailto:s.rothery@imperial.ac.uk> Website: http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ **************************************************************

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[FILM-Users 01074] Old ICTEM Analysis workstation
by Rothery, Stephen M 30 Jan '23

30 Jan '23

Dear All The old ICTEM analysis PC (currently located at SAF) is running windows 7 and as the college no longer allows these systems to be on the network, it has been removed from PPMS and is no longer available to users If you have been using this system in the past then there is a new ICTEM "power" workstation at ICTEM situated on level 3 office area. This is already bookable on PPMS and can be used directly or remotely - please use and book in the normal way If you require remote access to this PC then please e-mail me and I will send you instructions s.rothery(a)imperial.ac.uk<mailto:s.rothery@imperial.ac.uk> bw Steve FILM - Facility for Imaging by Light Microscopy ************************************************************** Steve Rothery FILM Technician Sir Alexander Fleming Building, desk 403 Imperial College London / South Kensington Exhibition Road London SW7 2AZ Tel +44-(0)207 594 9793 And ICTEM, (L-Block) Room 314 Hammersmith Campus Imperial College London Du Cane Road London, W12 0NN Tel. +44-(0)207 594 1669 E-mail s.rothery(a)imperial.ac.uk<mailto:s.rothery@imperial.ac.uk> Website: http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ **************************************************************

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[FILM-Users 01073] FW: streptavidin 488
by Rothery, Stephen M 27 Jan '23

27 Jan '23

Dear All If anyone can help Laura - see below, please contact her directly Nicastro, Laura l.nicastro19(a)imperial.ac.uk<mailto:l.nicastro19@imperial.ac.uk> DO NOT REPLY TO THIS E_MAIL Bw Steve Subject: streptavidin 488 Hi Steve, I am looking for a streptavidin dye (to use in combination with Isolectin B4) in 488. Even a small aliquot will be fine. Would you mind circulating this email around? Thank you very much. Best, Laura

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[FILM-Users 01072] TMRE dye
by Rothery, Stephen M 23 Jan '23

23 Jan '23

Dear All If anyone can help Alejandra – see below, please contact her directly Tomas Catala, Alejandra D D a.tomas-catala(a)imperial.ac.uk<mailto:a.tomas-catala@imperial.ac.uk> DO NOT REPLY TO THIS E_MAIL Bw Steve ___________________________ Please could you circulate the following request to the FILM members? Thanks BW Alejandra “We are in dire need of a small amount of TMRE dye to measure mitochondrial membrane potential. We have ordered some but are still waiting for it to be delivered. Does anyone have some that we could borrow? We will replace it as soon as ours gets delivered. Thanks" Dr Alejandra Tomas Senior Lecturer, Section of Cell Biology and Functional Genomics Director, MSc Applied Genomics Director, Imperial Membrane Receptor Network of Excellence Division of Diabetes, Endocrinology and Metabolism Department of Metabolism, Digestion and Reproduction Imperial College London Room 329, 3rd floor ICTEM Building, Hammersmith Campus, Du Cane Road, London W12 0NN, UK tel: +44 20 7594 3364 email: a.tomas-catala(a)imperial.ac.uk<mailto:a.tomas-catala@imperial.ac.uk> https://www.imperial.ac.uk/people/a.tomas-catala https://www.imperial.ac.uk/medicine/research-and-impact/groups/signalling-a… Twitter: @TomasLab_ICL

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[FILM-Users 01071] FW: Workshop: Spatial Analysis for Biological Imaging
by Rothery, Stephen M 11 Jan '23

11 Jan '23

FYI The Spatial Analysis for Biological Imaging workshop will be held at the Department of Mathematics, Imperial College London, South Kensington Campus, London, UK between March 29-30, 2023. Workshop Aim: Imaging technologies play an ever increasing role in biomedical and clinical investigations as they allow the observation of important spatial processes. While such imaging approaches have undergone major technological developments through, for example, the development of novel detectors and sample preparation approaches, the analysis of the resulting data has often lagged behind. This is even more problematic as the amount of data that can and is being generated is very large. Methods for spatial analysis, including spatial statistics, machine learning, and topological data analysis, hold significant promise to be able to resolve many outstanding analytical problems. The purpose of this workshop is to provide a means for leading researchers in bioimaging, digital pathology and histology to present their latest findings and also to, for the first time, bring these communities together as it is expected that exchange of ideas could be of major benefit to all sides. Confirmed invited speakers: * Carolina Wählby, Uppsala University * Dylan Owen, University of Birmingham * Thibault Lagache, Institut Pasteur * Anthea Monod, Imperial College London * Anca Grapa, Institute of Cancer Research, London * Daniel Davis, Imperial College London * Sripad Ram, Pfizer, San Diego Contributed talks and posters are very welcome, and abstract submission is currently open. Further details, including registration, can be found at our website https://www.quantitativebioimaging.com/qbi2023/sabi2023/ Places are very limited. Book now to avoid disappointment. This workshop is generously supported by the Quantitative Sciences Research Institute (QSRI), Imperial College London, and in collaboration with the Quantitative Bioimaging Society (QBI).

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[FILM-Users 01070] FILM Christmas closure
by Gaboriau, David C A 21 Dec '22

21 Dec '22

Dear users, As we near the end of the year, we wanted to thank you for coming to FILM to image your samples. The College is closing on Friday 23rd December, and re-opening on Tuesday 03rd January, and FILM will be closed as well. It is important that all instruments are shut down on Friday 23rd and not left running over the Christmas period. So please make sure you shut down the microscope when you finish your session on Friday, even if someone is booked after you, unless they have already turned up to use it. In the event that you are using a microscope during the closed period, the same applies, please make sure to shut down the microscope after your session. Many thanks for your cooperation, and we wish you a great Christmas and Holiday, and all the best wishes for the New Year, All the best, David and the FILM team ---------------------------------------------------- David Gaboriau, PhD, FRMS Interim FILM Facility Manager Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 408 +44 (0)20 7594 9793 d.gaboriau(a)imperial.ac.uk https://www.imperial.ac.uk/people/d.gaboriau http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ ----------------------------------------------------

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[FILM-Users 01069] FW: Urgent Anti-myc Antibody Request
by Rothery, Stephen M 15 Dec '22

15 Dec '22

Dear All If anyone can help Alex – see below, please contact him directly Bailey, Alexander <a.bailey18(a)imperial.ac.uk<mailto:a.bailey18@imperial.ac.uk>> DO NOT REPLY TO THIS E_MAIL Bw Steve -----Original Message----- From: Bailey, Alexander <a.bailey18(a)imperial.ac.uk> Sent: 15 December 2022 10:57 To: FILM Service <film-service(a)imperial.ac.uk> Subject: Urgent Anti-myc Antibody Request Hi, I hope this email finds you well - would you be able to forward this urgent antibody request around the FILM mailing list? We are in urgent need of a primary anti-myc antibody today after a delivery ‘mishap’ for an IFA, if anyone in the Department would be able to spare us an aliquot it would be greatly appreciated - we can of course provide replacement once the delivery finally makes its way here! Any species would be completely fine except for mouse, as our other primary antibodies are mouse. Any help would be greatly appreciated! Thanks so much, Best Wishes, Alex

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