- FILM-Users - imperial.ac.uk

[FILM-Users 01113] Number of people in SAF room 409 (WF3 and WF1): two users per microscope maximum
by Gaboriau, David C A 25 Aug '23

25 Aug '23

Dear users, There was a recent instance where 5-6 people were using WF3, when another user was using WF1. As this is quite a small room, please could I ask you to limit the number of users to TWO PER MICROSCOPE from now on? This is to ensure user safety to have space to move around the microscope, and comfort during your imaging sessions. Many thanks for your cooperation. All the best, David ---------------------------------------------------- David Gaboriau, PhD, FRMS Microscopy Specialist Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 408 +44 (0)20 7594 9793 d.gaboriau(a)imperial.ac.uk https://www.imperial.ac.uk/people/d.gaboriau http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ ----------------------------------------------------

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[FILM-Users 01112] LS1 laser cooling system
by Gaboriau, David C A 25 Aug '23

25 Aug '23

Dear users, This afternoon we found that the cooling system for LS1, circled in yellow in the attached photograph, had been turned off. It should be left on to ensure the lasers are cooled to 18oC and never turned off, as this can cause laser over-heating and damage to the lasers and microscope. Please DO NOT TURN IT OFF when shutting down the system. Many thanks for your cooperation. If you are not sure how to turn off the system, please look at the instructions in the folder on the desk or ask a member of FILM. All the best, David ---------------------------------------------------- David Gaboriau, PhD, FRMS Microscopy Specialist Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 408 +44 (0)20 7594 9793 d.gaboriau(a)imperial.ac.uk https://www.imperial.ac.uk/people/d.gaboriau http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ ---------------------------------------------------- [cid:323a7a8b-de4d-4601-86ee-c1d46c90b31d@GBRP265.PROD.OUTLOOK.COM]

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[FILM-Users 01111] Assistance Needed: Anti-MUC-2 Antibody Aliquot for Research
by Nechyporuk-Zloy, Volodymyr 24 Aug '23

24 Aug '23

Dear Colleagues, I hope this message finds you well. I am writing to seek your assistance on behalf of Mr. Jake Samuel; he is currently in need of an aliquot of the anti-MUC-2 antibody to further his ongoing research efforts. If you have the capacity to offer assistance or could point us in the right direction, we would be truly grateful for your support. Kindly reach out to Mr. Jake Samuel directly at j.samuel19(a)imperial.ac.uk if you can contribute or have any leads to share. Thank you for your time and consideration. Best regards, Volodymyr Dr. Volodymyr Nechyporuk-Zloy (FILM Facility Manager) Facility for Imaging by Light Microscopy Imperial College of Science, Technology and Medicine Sir Alexander Fleming Building South Kensington Campus London SW7 2AZ, UK Mobile: (+44) 7526275617 Twitter: @vnzloy LinkedIn: https://www.linkedin.com/in/volodymyr-nechyporuk-zloy/ Company Web: https://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscop…

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[FILM-Users 01110] CF4 : no 405nm and no 543nm lasers
by Gaboriau, David C A 03 Aug '23

03 Aug '23

Dear users, Yesterday a Leica engineer tried to fit in the 405nm they sourced from another microscope abroad, but unfortunately he could not complete the task. He will have to come back another day. While there, he confirmed that the 543nm laser is not functioning and will have to be replaced. CF4 is functioning, but without these two lasers at the moment, we are sorry to say. We are working with Leica to resolve this. Apologies for the inconvenience, All the best, David ---------------------------------------------------- David Gaboriau, PhD, FRMS Microscopy Specialist Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 408 +44 (0)20 7594 9793 d.gaboriau(a)imperial.ac.uk https://www.imperial.ac.uk/people/d.gaboriau http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ ----------------------------------------------------

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[FILM-Users 01109] Cakes and drinks for Steve's retirement - Wednesday 02nd
by Gaboriau, David C A 01 Aug '23

01 Aug '23

Dear all, We are having cakes and drinks tomorrow for Steve's retirement, please join us if you are free! * Wednesday 02nd August, from 2pm * SAF building, on level 3 All the best, David ---------------------------------------------------- David Gaboriau, PhD, FRMS Microscopy Specialist Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 408 +44 (0)20 7594 9793 d.gaboriau(a)imperial.ac.uk https://www.imperial.ac.uk/people/d.gaboriau http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ ----------------------------------------------------

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[FILM-Users 01108] FW: Cardiac Troponin C antibody
by Rothery, Stephen M 27 Jul '23

27 Jul '23

Dear All If anyone can help Yee- see below - please contact her directly Ho, Yee Mang yee.ho22(a)imperial.ac.uk<mailto:yee.ho22@imperial.ac.uk> DO NOT REPLY TO THIS E_MAIL Bw steve From: Ho, Yee Mang <yee.ho22(a)imperial.ac.uk> Sent: 27 July 2023 14:41 To: Rothery, Stephen M <s.rothery(a)imperial.ac.uk> Subject: Cardiac Troponin C antibody Hi Steve, I urgently need a cardiac troponin C antibody- rabbit host. Please could you circulate this to see if anybody has some spare that I could use? Kind regards, Yee Yee Mang Ho | CID: 01711838 MSc Genes, Drugs and Stem Cells Student National Heart & Lung Institute | Faculty of Medicine

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[FILM-Users 01107] CF6 is working again - CF4 new 405nm laser will be installed
by Gaboriau, David C A 24 Jul '23

24 Jul '23

Dear users, I am pleased to say CF6 is working again. A Leica engineer was able to fix the software/hardware issue remotely this morning. On other confocal news, Leica has also been able to source a replacement 405nm laser for CF4, so it will be installed on Wednesday 02nd August. All the best, David ---------------------------------------------------- David Gaboriau, PhD, FRMS Microscopy Specialist Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 408 +44 (0)20 7594 9793 d.gaboriau(a)imperial.ac.uk https://www.imperial.ac.uk/people/d.gaboriau http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ ----------------------------------------------------

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[FILM-Users 01106] CF6 out of use - can not connect to scanner
by Gaboriau, David C A 21 Jul '23

21 Jul '23

Dear users, We are having problems with CF6, it can not connect to the scanner,. That means the microscope is out of use for the moment. We have raised an urgent ticket with Leica and will keep you updated. Apologies for the inconvenience. All the best, David ---------------------------------------------------- David Gaboriau, PhD, FRMS Microscopy Specialist Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 408 +44 (0)20 7594 9793 d.gaboriau(a)imperial.ac.uk https://www.imperial.ac.uk/people/d.gaboriau http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ ----------------------------------------------------

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[FILM-Users 01104] Scratch2 drive not connecting
by Rothery, Stephen M 17 Jul '23

17 Jul '23

Dear all There is a problem connecting to the Scratch drive at the moment ICT are trying to resolve the problem Bw Steve FILM - Facility for Imaging by Light Microscopy ************************************************************** Steve Rothery FILM Technician Sir Alexander Fleming Building, desk 403 Imperial College London / South Kensington Exhibition Road London SW7 2AZ Tel +44-(0)207 594 9793 And ICTEM, (L-Block) Room 314 Hammersmith Campus Imperial College London Du Cane Road London, W12 0NN Tel. +44-(0)207 594 1669 E-mail s.rothery(a)imperial.ac.uk<mailto:s.rothery@imperial.ac.uk> Website: http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ **************************************************************

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[FILM-Users 01103] FW: LYVE-1 or PROX-1
by Rothery, Stephen M 21 Jun '23

21 Jun '23

Dear All If anyone can help Jake- see below - please contact him directly Samuel, Jake j.samuel19(a)imperial.ac.uk<mailto:j.samuel19@imperial.ac.uk> DO NOT REPLY TO THIS E_MAIL Bw steve From: Samuel, Jake <j.samuel19(a)imperial.ac.uk> Sent: 21 June 2023 13:50 To: Rothery, Stephen M <s.rothery(a)imperial.ac.uk> Subject: LYVE-1 or PROX-1 Hi Steve, I am after an aliquot of an antibody targeting LYVE-1 or PROX-1. Would you be able to circulate this email to see if anyone has some spare? Cheers, Jake

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