- FILM-Users - imperial.ac.uk

[FILM-Users 00993] New Stellaris 8 at Hammersmith
by Rothery, Stephen M 21 Oct '21

21 Oct '21

Dear All The new Leica Stellaris 8 confocal is now up and running https://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscop… If you would like training on this instrument please contact Steve Rothery or a member of the FILM staff Best wishes Steve FILM - Facility for Imaging by Light Microscopy ************************************************************** Steve Rothery FILM Technician Sir Alexander Fleming Building, desk 403 Imperial College London / South Kensington Exhibition Road London SW7 2AZ Tel +44-(0)207 594 9793 And ICTEM, (L-Block) Room 314 Hammersmith Campus Imperial College London Du Cane Road London, W12 0NN Tel. +44-(0)207 594 1669 E-mail s.rothery(a)imperial.ac.uk<mailto:s.rothery@imperial.ac.uk> Website: http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ **************************************************************

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[FILM-Users 00991] FW: Imaris workshop on November 11th and 12th - now with attachment
by Bruckbauer, Andreas 13 Oct '21

13 Oct '21

Sorry, this time with the right attachment with link to sign up! Dear FILM-users, We will have access to a license of the Imaris image analysis software which is shared between FILM and the Bioengineering department. This is very powerful software for 3D and 4D image analysis including feature segmentation, tracking, lineage tracing and filament tracing. To launch the software Bitplane is organising a workshop with a seminar (hybrid event, online and offline on November 11th) and 1 on 1 sessions to analyse your (pre-submitted) data on November 12th, please see the attached flyer for info on how to sign up. The on-site seminar has limited spaces and is for Imperial College attendants only, face masks are required. But it will be streamed for online viewing. Best wishes Andreas ---------------------------------------------------- Dr Andreas Bruckbauer FILM facility manager Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 401 +44 (0)20 7594 2023 a.bruckbauer(a)imperial.ac.uk<mailto:a.bruckbauer@imperial.ac.uk> http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ http://www.imperial.ac.uk/people/a.bruckbauer ----------------------------------------------------

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[FILM-Users 00990] Imaris workshop on November 11th and 12th
by Bruckbauer, Andreas 13 Oct '21

13 Oct '21

Dear FILM-users, We will have access to a license of the Imaris image analysis software which is shared between FILM and the Bioengineering department. This is very powerful software for 3D and 4D image analysis including feature segmentation, tracking, lineage tracing and filament tracing. To launch the software Bitplane is organising a workshop with a seminar (hybrid event, online and offline on November 11th) and 1 on 1 sessions to analyse your (pre-submitted) data on November 12th, please see the attached flyer for info on how to sign up. The on-site seminar has limited spaces and is for Imperial College attendants only, face masks are required. But it will be streamed for online viewing. Best wishes Andreas ---------------------------------------------------- Dr Andreas Bruckbauer FILM facility manager Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 401 +44 (0)20 7594 2023 a.bruckbauer(a)imperial.ac.uk<mailto:a.bruckbauer@imperial.ac.uk> http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ http://www.imperial.ac.uk/people/a.bruckbauer ----------------------------------------------------

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[FILM-Users 00989] Research Technician in microscopy
by Bruckbauer, Andreas 08 Oct '21

08 Oct '21

FYI: Exciting opportunity for a research technician with Richard Burt and Cristina Lo Celso. The post will involve intravital imaging to study the treatment resistant population in Acute Lymphoblastic Leukaemia, see attachment for further details. Enquiries to r.burt(a)imperial.ac.uk<mailto:r.burt@imperial.ac.uk> please. Best wishes Andreas ---------------------------------------------------- Dr Andreas Bruckbauer FILM facility manager Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 401 +44 (0)20 7594 2023 a.bruckbauer(a)imperial.ac.uk<mailto:a.bruckbauer@imperial.ac.uk> http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ http://www.imperial.ac.uk/people/a.bruckbauer ----------------------------------------------------

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[FILM-Users 00988] Seminar talk: High-resolution intravital microscopy - meet the speaker - October 27th
by Bruckbauer, Andreas 07 Oct '21

07 Oct '21

Dear all, The upcoming Cardio Respiratory Interface (CRI) external seminar will feature a talk from Assistant Professor Jose Javier Bravo-Cordero on 'High-resolution intravital microscopy reveals the plastic behaviour of dormant cancer cells and their niches' on Wednesday 27th October 2021 (12:00 - 1:00 pm). Please click on the link below for the Teams Meeting link and to find out more details about the talk: https://www.imperial.ac.uk/events/140110/high-resolution-intravital-microsc… [cid:image001.png@01D7BB7D.D2425E70] If any PIs or fellows would like to talk to the speaker individually after his talk, could you please contact Dr Sek-Shir Cheong (sek-shir.cheong(a)imperial.ac.uk<mailto:sek-shir.cheong@imperial.ac.uk>) by the end of this Friday, 8 October 2021. Thanks everyone. Best wishes, Sek-Shir

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[FILM-Users 00987] Seminar talk on Intravital Microscopy, Wednesday October 27th on Teams
by Bruckbauer, Andreas 30 Sep '21

30 Sep '21

FYI: [cid:image001.jpg@01D7B614.FBF68FC0] Join on Teams<https://teams.microsoft.com/l/meetup-join/19%3af2502db818d94b33803ec909ff1e…> or contact Dr Sek Shir Cheong (sek-shir.cheong(a)imperial.ac.uk<mailto:sek-shir.cheong@imperial.ac.uk>) or Dr Charlotte Dean (c.dean(a)imperial.ac.uk<mailto:c.dean@imperial.ac.uk>) with any queries. Find out more <https://www.imperial.ac.uk/events/140110/high-resolution-intravital-microsc…> about the speaker. Best wishes, Sek Shir ---------------------------------------------------- Dr Andreas Bruckbauer FILM facility manager Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 401 +44 (0)20 7594 2023 a.bruckbauer(a)imperial.ac.uk<mailto:a.bruckbauer@imperial.ac.uk> http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ http://www.imperial.ac.uk/people/a.bruckbauer ----------------------------------------------------

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[FILM-Users 00986] Hammersmith Widefield HWF1 Move
by Rothery, Stephen M 24 Sep '21

24 Sep '21

Dear All Hammersmith Widefield HWF1 Move [cid:image002.jpg@01D7B14E.0CECF420]This morning the Hammersmith widefield microscope was moved from the 3rd floor to room 521 on the 5th floor (north side corridor) to make room for a new confocal microscope. Access !! Important !! if you are planning to use the microscope in the next few weeks please check your card access before your session to make sure your access works. We are trying to update users' access but if your card does not work please contact Steve (s.rothery(a)imperial.ac.uk<mailto:s.rothery@imperial.ac.uk>) Log in We were hoping that everything would be in place before the move so that the disruption was minimal, however there will be no network connection for about a week. This will mean that your login won't work and your profiles can't be read. As a temporary measure to get around this, everyone will use the same local login (details will be on the microscope). You will still be able to load and re-use your images as before, however, please make sure you save your data to your own folder on the data D or R drive. Also, the scratch transfer drive will not be available. To get around this we have provided two external hard drives to allow you to copy the data (please return them when you have copied your data), or you can bring your own external hard drive if you have one. You will not be able to access PPMS or the internet through the microscope PC so you will have to make booking changes or check the booking through your phone or from your own desktop until the network is sorted CO2 There will also be no CO2 for the time being until we sort out a supply. Start-up There will be new start-up instructions which will be sent out later and there will also be a copy by the microscope. Please follow these carefully. Please let us know if this causes a problem for you Apologies for the disruption Many thanks Steve FILM - Facility for Imaging by Light Microscopy ************************************************************** Steve Rothery FILM Technician Sir Alexander Fleming Building, desk 403 Imperial College London / South Kensington Exhibition Road London SW7 2AZ Tel +44-(0)207 594 9793 And ICTEM, (L-Block) Room 314 Hammersmith Campus Imperial College London Du Cane Road London, W12 0NN Tel. +44-(0)207 594 1669 E-mail s.rothery(a)imperial.ac.uk<mailto:s.rothery@imperial.ac.uk> Website: http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ **************************************************************

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[FILM-Users 00985] speaker test
by Bruckbauer, Andreas 22 Sep '21

22 Sep '21

Dear FILM-users, The music coming out of the speakers at SAF today is part of a test of the fire-alarm system and will continue for today and tomorrow. Sorry if this is annoying. Best wishes Andreas ---------------------------------------------------- Dr Andreas Bruckbauer FILM facility manager Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 401 +44 (0)20 7594 2023 a.bruckbauer(a)imperial.ac.uk<mailto:a.bruckbauer@imperial.ac.uk> http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ http://www.imperial.ac.uk/people/a.bruckbauer ----------------------------------------------------

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[FILM-Users 00984] LSCN Symposium 2021
by Bruckbauer, Andreas 14 Sep '21

14 Sep '21

Please see the stem cell symposium: [cid:0b3c6306-1de4-42be-b3d3-628de0599477@GBRP123.PROD.OUTLOOK.COM] On 6 Sep 2021, at 17:23, Lscn Admin <lscn-admin(a)lscn.org.uk<mailto:lscn-admin@lscn.org.uk>> wrote: Dear all, Just a reminder that there is an LSCN Committee Meeting tomorrow at 3pm (on zoom). Join Zoom Meeting https://ucl.zoom.us/j/92325520636?pwd=dHNSWFIrRzhJWkVnekNCV2N2R05adz09 Meeting ID: 923 2552 0636 Passcode: 090099 Please also continue to promote our annual symposium<https://lscn.crick.ac.uk/?page_id=1075> in your labs, at your institutions and online - abstract submission is open until 15th September. Best wishes, Rob

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[FILM-Users 00983] Masson's Trichrome
by Bruckbauer, Andreas 03 Sep '21

03 Sep '21

The Green group is looking for some Masson's trichrome stain, since the lead time is really long at the moment. Do any of you have some spare one they would be happy to share? Contact @Portillo Lara, Roberto<mailto:r.portillo-lara@imperial.ac.uk> ---------------------------------------------------- Dr Andreas Bruckbauer FILM facility manager Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 401 +44 (0)20 7594 2023 a.bruckbauer(a)imperial.ac.uk<mailto:a.bruckbauer@imperial.ac.uk> http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ ----------------------------------------------------

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