- FILM-Users - imperial.ac.uk

[FILM-Users 00982] Nestin?
by Bruckbauer, Andreas 31 Aug '21

31 Aug '21

If anybody can help Catalina, please contact her directly: Can you help me please asking around if anyone could spare with me 10ul of Nestin - Neural Stem Cell Marker, that reacts with mouse/rat tissue please?. This is for a last experiment. Many thanks, Catalina Catalina Vallejo Giraldo, PhD Research Associate, Department of Bioengineering Imperial College London B211 Bessemer Phone: 07 365877249 Email: cvallejo(a)imperial.ac.uk<mailto:cvallejo@imperial.ac.uk> ---------------------------------------------------- Dr Andreas Bruckbauer FILM facility manager Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 401 +44 (0)20 7594 2023 a.bruckbauer(a)imperial.ac.uk<mailto:a.bruckbauer@imperial.ac.uk> http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ http://www.imperial.ac.uk/people/a.bruckbauer ----------------------------------------------------

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[FILM-Users 00981] CytoData 2021
by Bruckbauer, Andreas 31 Aug '21

31 Aug '21

FYI: ------------------- https://www.bit.bio/events/cytodata2021<https://eur03.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.bit.b…> CytoData 2021: October 13-15th on Zoom The sixth annual CytoData meeting will be virtually hosted by bit.bio 13-15th October 2021! CytoData unites microscopists and researchers mining cell imaging data to define novel and unique ways to tackle challenges in image analysis. Since 2016 CytoData events have proven incredibly exciting and we hope to replicate the virtues of this meeting once again in 2021! The meeting is composed of four sessions including Methods, Primary Cells, Stem Cells, and Academia/Pharma/CRO, all featuring 10-minute talks. We have a fantastic line-up of invited speakers so PLEASE register and keep a close eye on your inbox! Along with our invited speakers, we encourage both budding image analysts and veterans in the field to submit an abstract. As in 2020, CytoData is to be held virtually this year over three days and will include the popular Hackathon, where participants will be able to work together to engineer novel solutions to tackle challenges in image analysis. To register or submit an abstract please visit the CytoData 2021 website<https://eur03.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.bit.b…>. Find out more about the CytoData Society<https://eur03.safelinks.protection.outlook.com/?url=https%3A%2F%2Fsociety.c…> and bit.bio<https://eur03.safelinks.protection.outlook.com/?url=http%3A%2F%2Fbit.bio%2F…>. The CytoData team at bit.bio -- Fuad Mosis Scientist, Cellular Phenotyping T +44 (0)1223 787297 fuad.mosis(a)bit.bio<mailto:%2520%257BEmail1%257D> The Dorothy Hodgkin Building Babraham Research Campus Cambridge CB22 3FH United Kingdom info(a)bit.bio<mailto:%2520%257BEmail1%257D> | www.bit.bio<mailto:%2520%257BEmail1%257D> Follow us [https://drive.google.com/uc?id=1BYBY4OmAbHEC5goPYoDkRer0zmNq9qE-&export=dow…]<https://twitter.com/bitbio> [https://drive.google.com/uc?id=1yZAaYIkGPgRsqs8p010PkenB5eKi8bTA&export=dow…]<https://www.linkedin.com/company/bitbioltd/> [bit.bio]<http://www.bit.bio/> Notice: This message is the property of Bit Bio Ltd and contains information that may be confidential and/or privileged. If you are not the intended recipient, you should not use, disclose or take any action based on this message. If you have received this transmission in error, please immediately contact the sender by return e-mail and delete this e-mail, and any attachments, from any computer. ---------------------------------------------------- Dr Andreas Bruckbauer FILM facility manager Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 401 +44 (0)20 7594 2023 a.bruckbauer(a)imperial.ac.uk<mailto:a.bruckbauer@imperial.ac.uk> http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ http://www.imperial.ac.uk/people/a.bruckbauer ----------------------------------------------------

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[FILM-Users 00980] FW: In urgent need of hCD14 cell separation beads
by Rothery, Stephen M 20 Aug '21

20 Aug '21

Can anyone help Maria – please contact her directly if you can – see below PLEASE DO NOT REPLY TO THIS E_MAIL Dear FILM community I’m in urgent need of human CD14 micro beads from miltenyi (130-050-201), which will be needed on Monday. If you have these beads and can spare some, could you please contact me: m.weinert13(a)ic.ac.uk<mailto:m.weinert13@ic.ac.uk>. I will be able to return them latest on Thursday next week. All the best Maria

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[FILM-Users 00979] ISRMN Intravital Microscopy Workshop: Registration and abstract submission
by Rothery, Stephen M 09 Aug '21

09 Aug '21

PLEASE DO NOT REPLY TO THIS E-MAIL, THANK-YOU Dear colleagues, ISRMN is organizing a Intravital Microscopy Workshop, bringing together researchers from Imperial and abroad in a series of talks and flash talks and networking. This workshop will be a great opportunity for early career researchers to present their work and interact with experts from a variety of fields. There will be prizes for the best flash talks to be used towards conference registration fees. Date: 22nd September 2021, 2.30-6pm Location: * South Kensington campus, Sir Alexander Fleming Building, G34 Lecture Theatre * Online session will also be available ABSTRACT SUBMISSION: * Abstracts should not be longer than 250 words. * Abstract should be sent to isrmncontact(a)imperial.ac.uk<mailto:isrmncontact@imperial.ac.uk> in PDF format. * Deadline for abstract submission: 1st September 2021 * Announcement of the selected abstracts: 8th September 2021 * Prizes of 150£ for the best flash talks to be used towards conference registration fees. PRELIMINARY PROGRAM Talks (2.30-5pm): * Professor Valentina Greco, Yale School of Medicine - "Tissue dynamics and functions captured by imaging the skin of live mice" * Dr Giovanni Sena, Imperial College London - "Light-sheet microscopy and plant root regeneration" * Dr Daniel Stuckey, University College of London - "Using in vivo imaging to guide cardiac regeneration" * Selected flash talks Networking Session (5-6pm): Drinks and nibbles will be provided Free Registration: https://www.imperial.ac.uk/stem-cell-regenerative-medicine-network/about/bi… [http://www.imperial.ac.uk/T4Assets/favicon-196x196.png]<https://www.imperial.ac.uk/stem-cell-regenerative-medicine-network/about/bi…> 22 September 2021 Intravital Microscopy Workshop<https://www.imperial.ac.uk/stem-cell-regenerative-medicine-network/about/bi…> Registration and abstract submission are now open! Please fill the registration form bellow! www.imperial.ac.uk<http://www.imperial.ac.uk>   Best regards, The ISRMN Coordination Team

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[FILM-Users 00978] Plate reader in SAFB?
by Bruckbauer, Andreas 06 Aug '21

06 Aug '21

Dear FILM -users, Nupur Kohli from the Mechanical Engineering Department is looking for a plate reader in the SAF building that she can use. She wants to do a colorimetric calcium assay measuring absorbance at 575nm. Please contact her directly If you can help her to locate such an instrument. n.kohli(a)imperial.ac.uk<mailto:n.kohli@imperial.ac.uk> Best wishes Andreas ---------------------------------------------------- Dr Andreas Bruckbauer FILM facility manager Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 401 +44 (0)20 7594 2023 a.bruckbauer(a)imperial.ac.uk<mailto:a.bruckbauer@imperial.ac.uk> http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ http://www.imperial.ac.uk/people/a.bruckbauer ----------------------------------------------------

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[FILM-Users 00977] Maximum occupancy in microscope rooms increased
by Bruckbauer, Andreas 29 Jul '21

29 Jul '21

Dear users of the FILM facility, We are taking part in a trial 1m+ to allow supervised use of our microscopes (2 people on one microscope) 409= 2 people 410 = 2 people 408 = 6 people 527= 2 people 530 = 2 people These represent the maximum new occupancies, if e.g. training or supervised use of microscopes is to take place (2 people at one microscope), but unlikely to reach these during normal everyday use In all cases we will trial 1m + mitigation on the basis that: * All usage must be booked (and booking systems updated accordingly) * Room occupancy must not exceed new agreed limits (Signage is updated). * Disposable face masks (not face coverings) must be worn in these labs as mitigation due to <2M distancing * Those working in these rooms must continue to remain socially distanced, and avoid pinch points or working in close proximity to others unless necessary. These are pilots, and will be reviewed with the right to withdraw these arrangements should we need to do so (due to non-compliance or changes in college guidance). Best wishes Andreas ---------------------------------------------------- Dr Andreas Bruckbauer FILM facility manager Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 401 +44 (0)20 7594 2023 a.bruckbauer(a)imperial.ac.uk<mailto:a.bruckbauer@imperial.ac.uk> http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ http://www.imperial.ac.uk/people/a.bruckbauer ----------------------------------------------------

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[FILM-Users 00976] Alexa Fluor 488 goat-anti-mouse secondary
by Bruckbauer, Andreas 28 Jul '21

28 Jul '21

Sorry for the mass-email, does anybody have 32 ul of Alexa Fluor(r) 488 goat-anti-mouse secondary for Catalina Vallejo Giraldo? She has ordered a new vial, but it has not arrived just yet. Please contact her directly if you can help: c.vallejo-giraldo(a)imperial.ac.uk<mailto:c.vallejo-giraldo@imperial.ac.uk> Best wishes Andreas ---------------------------------------------------- Dr Andreas Bruckbauer FILM facility manager Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 401 +44 (0)20 7594 2023 a.bruckbauer(a)imperial.ac.uk<mailto:a.bruckbauer@imperial.ac.uk> http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ http://www.imperial.ac.uk/people/a.bruckbauer ----------------------------------------------------

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[FILM-Users 00975] Confocal Application Specialist role at Leica
by Bruckbauer, Andreas 26 Jul '21

26 Jul '21

Dear all, There is an exciting position advertised for a Confocal Application Specialist with Leica, this would be ideal for someone reaching the end of their PhD and wondering what their next step could be. This position especially interesting because of the partnership between Leica and Imperial. https://danaher.wd1.myworkdayjobs.com/LeicaMicrosystemsJobs/job/London-Unit…<https://urldefense.proofpoint.com/v2/url?u=https-3A__danaher.wd1.myworkdayj…> https://www.imperial.ac.uk/news/213593/partnership-between-imperial-leica-i… best wishes Andreas ---------------------------------------------------- Dr Andreas Bruckbauer FILM facility manager Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 401 +44 (0)20 7594 2023 a.bruckbauer(a)imperial.ac.uk<mailto:a.bruckbauer@imperial.ac.uk> http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ http://www.imperial.ac.uk/people/a.bruckbauer ----------------------------------------------------

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[FILM-Users 00974] Reminder Data Deletion Scratch 2 drive
by Rothery, Stephen M 23 Jul '21

23 Jul '21

Dear All The scratch2 drive is getting full, so we need to delete older files from it We will delete files older than the 1st October 2020 next week NB This will include data files (eg exel dat etc) Please make sure you have everything backed up from before then Also you are reminded that the scratch drive is for transferring FILM data only It is not for long term storage or for users to store other data and programs If you are working offsite then scratch can be accessed either through VPN or using the remote gateway to logon to you work PC Many thanks Steve FILM - Facility for Imaging by Light Microscopy ************************************************************** Steve Rothery FILM Technician Sir Alexander Fleming Building, desk 403 Imperial College London / South Kensington Exhibition Road London SW7 2AZ Tel +44-(0)207 594 9793 And ICTEM, (L-Block) Room 314 Hammersmith Campus Imperial College London Du Cane Road London, W12 0NN Tel. +44-(0)207 594 1669 E-mail s.rothery(a)imperial.ac.uk<mailto:s.rothery@imperial.ac.uk> Website: http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ **************************************************************

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[FILM-Users 00973] Antibody help Ki67 (mouse) and choline acetyl transferase (CHAT) antibodies.
by Rothery, Stephen M 01 Jul '21

01 Jul '21

Dear All If anyone can help Neda, please contact her directly - see below DO NOT REPLY TO THIS E-MAIL Mohammadi, Neda neda.mohammadi18(a)imperial.ac.uk<mailto:neda.mohammadi18@imperial.ac.uk> Dear Steve, Could I ask that you share an antibody request please, if possible? I am looking for Ki67 (mouse) and choline acetyl transferase (CHAT) antibodies. Thank you very much, Neda FILM - Facility for Imaging by Light Microscopy ************************************************************** Steve Rothery FILM Technician Sir Alexander Fleming Building, desk 403 Imperial College London / South Kensington Exhibition Road London SW7 2AZ Tel +44-(0)207 594 9793 And ICTEM, (L-Block) Room 314 Hammersmith Campus Imperial College London Du Cane Road London, W12 0NN Tel. +44-(0)207 594 1669 E-mail s.rothery(a)imperial.ac.uk<mailto:s.rothery@imperial.ac.uk> Website: http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ **************************************************************

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