- FILM-Users - imperial.ac.uk

[FILM-Users 01014] FW: Help please
by Rothery, Stephen M 08 Mar '22

08 Mar '22

Dear All If anyone can help Aida, please contact her directly Di Gregorio Pena, Aida aida.digregorio(a)imperial.ac.uk<mailto:aida.digregorio@imperial.ac.uk> DO NOT REPLY TO THIS E_MAIL Bw Steve Dear Stephen, Could you please ask the FILM users if anyone has a spare 2uL of a secondary antibody; Alexa 647 anti rat? Many thanks, Aida

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[FILM-Users 01013] Update of COVID rules for the FILM facility
by Bruckbauer, Andreas 07 Mar '22

07 Mar '22

Dear FILM users, After speaking to our Health and Safety advisor we have made some changes to our COVID-19 rules for the FILM facility. * As it is now believed that virus transmission through contact with surfaces is less of a problem, we will remove the cling-film from keyboards and eye-pieces. We keep the 70% ethanol so that you can still clean the surfaces you touch. * We will remove the 30 min break between the sessions as we believe that our rooms are well ventilated. We keep the occupancy limit, general 1 m distancing and the requirement to wear face coverings to be on the safe side. The new rules are attached and will be presented when you book a microscope on PPMS. Best wishes Andreas ---------------------------------------------------- Dr Andreas Bruckbauer FILM facility manager Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 401 +44 (0)20 7594 2023 a.bruckbauer(a)imperial.ac.uk<mailto:a.bruckbauer@imperial.ac.uk> http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ http://www.imperial.ac.uk/people/a.bruckbauer ----------------------------------------------------

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[FILM-Users 01012] Help on microscopes
by Bruckbauer, Andreas 24 Feb '22

24 Feb '22

Dear FILM-users, We try our best to be available for help with the microscopes during normal working hours 9:00 - 17:00. But with currently 4 FILM staff spread over 3 sites it is not always possible to provide immediate assistance, especially when staff are away because of e.g. sickness, holidays or conferences. So please confirm availability when you know in advance that you will need us, many users do this and it has worked quite well so far. We often do user-training or more complex experiments and it is not always possible to interrupt these when help is needed elsewhere. Please email Cristina Lo Celso as head of the facility or any of the members of the FILM steering committee (contacts under people on our website) if you are concerned about staffing levels in FILM. All the best for you and your imaging sessions! Andreas ---------------------------------------------------- Dr Andreas Bruckbauer FILM facility manager Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 401 +44 (0)20 7594 2023 a.bruckbauer(a)imperial.ac.uk<mailto:a.bruckbauer@imperial.ac.uk> http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ http://www.imperial.ac.uk/people/a.bruckbauer ----------------------------------------------------

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[FILM-Users 00992] Fridge in widefield microscope room 409
by Bruckbauer, Andreas 23 Feb '22

23 Feb '22

Dear FILM-users, We will get a new fridge in room 409 soon, if you still have samples in the old fridge, please remove them as soon as possible. In case you need to store them in the fridge for your experiment, they must be labelled with your name and date, so that we can transfer them safely to the new fridge. All unlabelled or old samples (older than 1 month) will be disposed of. Best wishes Andreas ---------------------------------------------------- Dr Andreas Bruckbauer FILM facility manager Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 401 +44 (0)20 7594 2023 a.bruckbauer(a)imperial.ac.uk<mailto:a.bruckbauer@imperial.ac.uk> http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ http://www.imperial.ac.uk/people/a.bruckbauer ----------------------------------------------------

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[FILM-Users 01010] FW: PDRA position at IRB Barcelona
by Bruckbauer, Andreas 21 Feb '22

21 Feb '22

Dear all, please see Chris’ email for the position in Barcelona, best wishes, Andreas From: UK Euro-BioImaging discussion list <UK-EUROBIOIMAGING-PROJECT(a)JISCMAIL.AC.UK> On Behalf Of Dunsby, Christopher W Sent: 21 February 2022 09:46 To: UK-EUROBIOIMAGING-PROJECT(a)JISCMAIL.AC.UK Subject: PDRA position at IRB Barcelona Dear all, Please see the attached PDRA job opportunity to work with Julien Colombelli at the IRB Barcelona on applying light-sheet microscopy to imaging 3D cancer organoid models. The project is part of a multi-site CRUK-funded grant joint with our group at Imperial College London. Best regards, Chris From: Julien Colombelli <julien.colombelli(a)irbbarcelona.org<mailto:julien.colombelli@irbbarcelona.org>> Sent: 18 February 2022 11:09 To: Dunsby, Christopher W <christopher.dunsby(a)imperial.ac.uk<mailto:christopher.dunsby@imperial.ac.uk>>; eduard.batlle <eduard.batlle(a)irbbarcelona.org<mailto:eduard.batlle@irbbarcelona.org>> Subject: advert postdoc position This email from julien.colombelli(a)irbbarcelona.org<mailto:julien.colombelli@irbbarcelona.org> originates from outside Imperial. Do not click on links and attachments unless you recognise the sender. If you trust the sender, add them to your safe senders list<https://spam.ic.ac.uk/SpamConsole/Senders.aspx> to disable email stamping for this address. -- Julien Colombelli Head of the Advanced Digital Microscopy Core Facility Institute for Research in Biomedicine - IRB Barcelona Barcelona Institute of Science and Technology - BIST Baldiri Reixac, 10. E-08028 Barcelona - Spain Tel: +349340-20451 -- Fax: +34934031116 julien.colombelli(a)irbbarcelona.org<mailto:julien.colombelli@irbbarcelona.org> http://adm.irbbarcelona.org/ Network of European Bioimage Analysts - NEUBIAS Chair - COST Action CA15124 www.neubias.org<http://www.neubias.org> ________________________________ UK-EUROBIOIMAGING Visit the website http://www.bioimaginguk.org To e-mail the group, send messages to UK-EUROBIOIMAGING-PROJECT(a)JISCMAIL.AC.UK<mailto:UK-EUROBIOIMAGING-PROJECT@JISCMAIL.AC.UK> To subscribe visit https://www.jiscmail.ac.uk/cgi-bin/webadmin?A0=UK-EUROBIOIMAGING-PROJECT Follow us on Twitter https://twitter.com/BioImagingUK ________________________________

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[FILM-Users 01009] FILM-staff today
by Bruckbauer, Andreas 18 Feb '22

18 Feb '22

Dear film-users, FILM-staff commute considerable distances to work, mostly by rail, and there is now a red weather warning in place for London today because of Storm Eunice, therefore we will unfortunately not be able to be on site. We will be working from home and can assist over e.g. Teams. Please check with your department regarding possible building closures and stay safe! Best wishes Andreas ---------------------------------------------------- Dr Andreas Bruckbauer FILM facility manager Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 401 +44 (0)20 7594 2023 a.bruckbauer(a)imperial.ac.uk<mailto:a.bruckbauer@imperial.ac.uk> http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ ----------------------------------------------------

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[FILM-Users 01008] FW: inquiry about Amphotericin B
by Rothery, Stephen M 08 Feb '22

08 Feb '22

Dear All If anyone can help Marieke - see below, please send her an e-mail Hoekstra, Marieke M B m.hoekstra(a)imperial.ac.uk<mailto:m.hoekstra@imperial.ac.uk> DO NOT REPLY TO THIS E-MAIL Hi Steve, Could you ask the FILMS users if anyone has a spare 5mL Amphotericin B? Thanks, Marieke Marieke Hoekstra Research Associate, Brancaccio Lab UK Dementia Research Institute at Imperial College London Department of Brain Sciences Tel | +44 (0)207 594 1550 Web | https://www.imperial.ac.uk/brancaccio-laboratory || http://www.imperial.ac.uk/people/m.hoekstra Address | E503 Burlington Danes | Hammersmith Hospital | Imperial College London Du Cane Rd | London W12 0NN

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[FILM-Users 01006] See the Hidden Workshop - Leica and Imperial College London - Friday 27th January
by Gaboriau, David C A 24 Jan '22

24 Jan '22

Dear Users, Please see below to register for a virtual workshop this Friday, organised by Leica with researchers from Imperial College London, focusing on high resolution imaging and AI powered image analysis: "We are pleased to invite you to the next virtual edition of our See the Hidden Workshop Series, examining the next generation of methodologies, techniques, and workflows that are helping to accelerate cancer research. In collaboration with Imperial College London (ICL), UK, this event will showcase how a multidisciplinary research approach can create innovative new ideas for the detection, prevention and, ultimately, the treatment of cancer. Through a series of scientific talks, Professor Chris Bakal, Dr. Vania Braga and Dr. Periklis Pantazis from ICL will present their cutting-edge cancer research and show how specialized microscopy techniques contribute to this. We’ll also take a closer look at the microscopy workflows used, with relevant product demonstrations in real-time. The program will focus on two exciting areas of microscopy – high-resolution optical imaging and artificial intelligence (AI) powered image analysis. Join us live to put your questions to the experts and get involved in the discussions." Link to register https://www2.leica-microsystems.com/webmail/149501/960632250/9755ab0c92e856… All the best, David ---------------------------------------------------- David Gaboriau, PhD, FRMS FILM Microscopy Specialist Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 408 +44 (0)20 7594 9793 d.gaboriau(a)imperial.ac.uk https://www.imperial.ac.uk/people/d.gaboriau http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ ----------------------------------------------------

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[FILM-Users 01005] !!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!! Data Deletion SCRATCH 2 !!!!!!!!!!!!!!!!!!!!
by Rothery, Stephen M 18 Jan '22

18 Jan '22

Dear All The scratch2 drive is getting full, so we need to delete older files from it We will delete files older than the 1st January 2021 next week NB This will include data files (eg exel data etc) Please make sure you have everything backed up from before then Also you are reminded that the scratch drive is for transferring FILM data only It is not for long term storage or for users to store other data and programs If you are working offsite then scratch can be accessed either through VPN or using the remote gateway to logon to you work PC Many thanks Steve FILM - Facility for Imaging by Light Microscopy ************************************************************** Steve Rothery FILM Technician Sir Alexander Fleming Building, desk 403 Imperial College London / South Kensington Exhibition Road London SW7 2AZ Tel +44-(0)207 594 9793 And ICTEM, (L-Block) Room 314 Hammersmith Campus Imperial College London Du Cane Road London, W12 0NN Tel. +44-(0)207 594 1669 E-mail s.rothery(a)imperial.ac.uk<mailto:s.rothery@imperial.ac.uk> Website: http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ **************************************************************

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[FILM-Users 01004] Glutaraldehyde solution for Tuesday/Wednesday?
by Bruckbauer, Andreas 21 Dec '21

21 Dec '21

Dear FILM-users, Can anybody spare 50ml of glutaraldehyde solution on Tuesday/Wednesday for Thomas Hall? Please contact him directly thomas.hall14(a)imperial.ac.uk<mailto:thomas.hall14@imperial.ac.uk> Best wishes Andreas ---------------------------------------------------- Dr Andreas Bruckbauer FILM facility manager Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 401 +44 (0)20 7594 2023 a.bruckbauer(a)imperial.ac.uk<mailto:a.bruckbauer@imperial.ac.uk> http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ http://www.imperial.ac.uk/people/a.bruckbauer ----------------------------------------------------

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