- FILM-Users - imperial.ac.uk

[FILM-Users 01024] FW: ZEISS Microscopy Image Contest 2022 | Submit Your Best Image Until May 15
by Bruckbauer, Andreas 22 Apr '22

22 Apr '22

FYI: See below if you want to have your image published in a calendar (please consider copyright or confidentiality and discuss with your supervisor): A. From: ZEISS Microscopy <communications(a)microscopy.zeiss.com> Sent: 21 April 2022 09:04 To: Bruckbauer, Andreas <a.bruckbauer(a)imperial.ac.uk> Subject: ZEISS Microscopy Image Contest 2022 | Submit Your Best Image Until May 15 This email from communications(a)microscopy.zeiss.com<mailto:communications@microscopy.zeiss.com> originates from outside Imperial. Do not click on links and attachments unless you recognise the sender. If you trust the sender, add them to your safe senders list<https://spam.ic.ac.uk/SpamConsole/Senders.aspx> to disable email stamping for this address. Do you take stunning microscopy images? Submit your best image now! The contest is open to all microscopy enthusiasts and professionals in nine application categories. ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ ‌ [Zeiss]<https://www.zeiss.com/> view online<https://pages.zeiss.com/index.php/email/emailWebview?mkt_tok=ODk2LVhNUy03OT…> ZEISS Microscopy Image Contest 2022 Submit Your Best Image Until May 15 [https://pages.zeiss.com/rs/896-XMS-794/images/imagecontest2022_header.png] Dear Andreas Bruckbauer, We are thrilled to announce the ZEISS Microscopy Image Contest 2022. Do you take stunning microscopy images? Submit your best image now! The contest is open to all microscopy enthusiasts and professionals in nine application categories. Images can be taken with any ZEISS microscope (light, X-ray or electron microscope). Deadline for entry is May 15, 2022. Submit Your Image<http://go2.zeiss.com/ODk2LVhNUy03OTQAAAGD6h7kWO9b98LHgGj_5mgILzwn1RI87vdeyF…> All participants will receive a copy of the ZEISS Microscopy Calendar 2023. On top of that, winners in each application category will receive their own ZEISS Terra® ED binoculars. To submit your image and read all FAQs and rules, visit our contest web page<http://go2.zeiss.com/ODk2LVhNUy03OTQAAAGD6h7kW14VDD0bU83Oh_c1N7KGf3IfA2I_TN…>. Happy imaging, Your ZEISS Microscopy Team Imprint Carl Zeiss Ltd. ZEISS House Building 1030 Cambourne Business Park Cambourne Cambridge CB23 6DW Cambridgeshire United Kingdom Phone: +44 (0) 1223 401 450 Email: customercare.uk(a)zeiss.com<mailto:customercare.uk@zeiss.com> Commercial register: 542141 VAT ID No. GB 232 5089 78 Dr Jochen Peter (Chairman) Paul Adderley (Managing Director) Fabian Heller (Director) Unsubscribe<http://go2.zeiss.com/dc/E4vivYkItfxUrzGOC-XNIU3IYVKkxlujH8RbPWh2MoOdoniyWJO…> | Data Protection<http://go2.zeiss.com/ODk2LVhNUy03OTQAAAGD6h7kWeznKYOyZcomab_O9d_XOnk4kS8IWU…> | Legal Notice<http://go2.zeiss.com/ODk2LVhNUy03OTQAAAGD6h7kWTdFi21UDyTS4HmBVlrGgZSADebm9L…>

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[FILM-Users 01023] FILM COVID rules
by Bruckbauer, Andreas 20 Apr '22

20 Apr '22

Dear FILM-users, Hope you had a good break and are keen to continue your imaging projects! We don't need special COVID 19 rules for the facility any more, the College rules apply: http://www.imperial.ac.uk/about/covid-19/ * Wear face coverings in crowded areas * Stay at home if you have symptoms of respiratory disease * Get fully vaccinated * Cover your coughs and sneezes * Respect personal space. All covid-related occupancy limits have been removed. Training sessions and assistance by FILM staff will count like 'crowded areas', so face coverings are required unless exempt. Best wishes Andreas ---------------------------------------------------- Dr Andreas Bruckbauer FILM facility manager Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 401 +44 (0)20 7594 2023 a.bruckbauer(a)imperial.ac.uk<mailto:a.bruckbauer@imperial.ac.uk> http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ http://www.imperial.ac.uk/people/a.bruckbauer ----------------------------------------------------

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[FILM-Users 01022] please switch the microscopes off
by Bruckbauer, Andreas 11 Apr '22

11 Apr '22

Dear FILM-users, If you do microscopy on the last day before the College closure (12/04) or during the closure days (if you have access), please switch the microscope off when you are finished, even when another person is booked, as they might not show up. Happy Easter from the FILM team! Andreas ---------------------------------------------------- Dr Andreas Bruckbauer FILM facility manager Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 401 +44 (0)20 7594 2023 a.bruckbauer(a)imperial.ac.uk<mailto:a.bruckbauer@imperial.ac.uk> http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ http://www.imperial.ac.uk/people/a.bruckbauer ----------------------------------------------------

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[FILM-Users 01021] ELMI 2022 in Turku
by Bruckbauer, Andreas 05 Apr '22

05 Apr '22

FYI: Great microscopy conference: ELMI 2022, Turku, Finland The 21st International European Light Microscopy Initiative<https://elmi2022.eu/> (ELMI) meeting is coming back in 2022 as an in-person meeting held in Turku, Finland (7-10 June 2022). ELMI 2022 will be a hybrid event, and a subset of the program, including the scientific presentation, will also be available online. Early bird deadline: 13 April 2022 Final registration deadline: 16 May 2022 Register here: https://elmi2022.eu/registration-and-prices Turku is the oldest city in Finland, situated by the beautiful archipelago of the Baltic Sea and the home of the Euro-BioImaging<https://www.eurobioimaging.eu/> headquarters. ELMI 2022 will update you on the latest light microscopy developments, research, techniques, and instrumentation. During the morning sessions, international leaders will give plenary lectures. During the afternoon sessions, company workshops will provide demos of the latest microscopy techniques and instruments. ELMI 2022 will continue the successful tradition of bringing together microscopy users, developers, core facility staff, and industry leaders by mixing high-quality scientific talks and posters with workshops and demos of the latest imaging technologies, not forgetting social activities. This year the ELMI gala diner will be held in Naantali, a nearby city part of the Finnish archipelago and home of the Moomin island. As with previous ELMI meetings, we will also organize the traditional academia versus industry football game as well as a satellite meeting dedicated to core facility staff. Consult the ELMI website for more information regarding the ELMI. We hope many of you will join us in Turku for ELMI 2022. [IMG_256] ---------------------------------------------------- Dr Andreas Bruckbauer FILM facility manager Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 401 +44 (0)20 7594 2023 a.bruckbauer(a)imperial.ac.uk<mailto:a.bruckbauer@imperial.ac.uk> http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ http://www.imperial.ac.uk/people/a.bruckbauer ----------------------------------------------------

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[FILM-Users 01020] [FACS] - User Group Meeting - Immunohenotyping by imaging
by Bruckbauer, Andreas 04 Apr '22

04 Apr '22

Dear FILM-users, The Flow facility has a presentation this Friday about Chip Cytometry - although it's called cytometry it's an imaging technique, please see attached flyer. Best wishes Andreas ---------------------------------------------------- Dr Andreas Bruckbauer FILM facility manager Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 401 +44 (0)20 7594 2023 a.bruckbauer(a)imperial.ac.uk<mailto:a.bruckbauer@imperial.ac.uk> http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ http://www.imperial.ac.uk/people/a.bruckbauer ----------------------------------------------------

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[FILM-Users 01019] FW: AlexaFlours for IgM
by Rothery, Stephen M 29 Mar '22

29 Mar '22

Dear All If anyone can help Callum - see below, please contact him directly Muirhead, Callum r.muirhead(a)imperial.ac.uk<mailto:r.muirhead@imperial.ac.uk> DO NOT REPLY TO THIS E_MAIL Bw Steve ----------------------------------------------------------------------------------------------------------------------------------- Hi Stephen, Do you have any AlexaFluor's that would work on IgM antibodies, any wavelength is acceptable but it would need to work with a mouse primary. Best wishes, Callum.M (he/him/they) Research Technician UK Dementia Research Institute Imperial College London 7th Floor - Sir Michael Uren Building 86 Wood Lane London W12 0BZ [cid:38cadc54-b0dd-43b2-8797-97b2431e0012]

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[FILM-Users 01018] Last chance to register your samples: Cell Discover 7 demo April 6th – 8th
by Bruckbauer, Andreas 21 Mar '22

21 Mar '22

Dear FILM-users Please let me know as soon as possible if you want to test this microscope with your sample! I need to finalize the schedule. ---------------- Zeiss offered us an on-site demo of their Cell Discover 7 microscope on April 6th – 8th at South Kensington. This is a high-end widefield system which also has a LSM 900 confocal unit with Airyscan2 (super-resolution, or live cell imaging). https://www.zeiss.com/microscopy/int/products/imaging-systems/celldiscovere… It should also allow automated workflows like overview imaging in widefield mode, locating objects of interest in x,y and z, followed by confocal imaging of these objects. The reason why we are interested in this is that they claim it is possible to image organoids at high resolution and in an automated workflow with this instrument. While there are limitations doing this with confocal microscopy and I would think this would be better done on a special light-sheet microscope, this demo could give us some answers. The positive aspect is that this is an all-in one box microscope, so it should be suitable for containment-level work (but not during the demo). I will also work for other samples like cells on coverslips, in dishes or on plates. Please let me know if you want to have your samples imaged during the demo which will take place in the SAF building at South Kensington. The time is limited (only 3days). Best wishes Andreas ---------------------------------------------------- Dr Andreas Bruckbauer FILM facility manager Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 401 +44 (0)20 7594 2023 a.bruckbauer(a)imperial.ac.uk<mailto:a.bruckbauer@imperial.ac.uk> http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ http://www.imperial.ac.uk/people/a.bruckbauer ----------------------------------------------------

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[FILM-Users 01017] FW: Antibody help request
by Rothery, Stephen M 16 Mar '22

16 Mar '22

Dear All If anyone can help Anne, please contact her directly Wolfes, Anne C <a.wolfes(a)imperial.ac.uk<mailto:a.wolfes@imperial.ac.uk>> DO NOT REPLY TO THIS E_MAIL Bw Steve -----Original Message----- From: Wolfes, Anne C <a.wolfes(a)imperial.ac.uk> Sent: 16 March 2022 15:33 To: Rothery, Stephen M <s.rothery(a)imperial.ac.uk> Subject: Antibody help request Hi Steve, I’m looking for an antibody for immunostaining fixed tissue against any of the following (NOT raised in mouse): - laminin B1 - neurexin 3a - Gapdh Any recommendations, example images, or even a small aliquot would be much appreciated! My goal is to stain the extracellular vs intracellular space in the suprachiasmatic nucleus, and the above options appear to be consistently expressed (i.e. levels don’t change during the day vs night). Many thanks, Anne

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[FILM-Users 01016] Cell Discover 7 demo April 6th – 8th
by Bruckbauer, Andreas 09 Mar '22

09 Mar '22

Dear FILM-users Zeiss offered us an on-site demo of their Cell Discover 7 microscope on April 6th – 8th at South Kensington. This is a high-end widefield system which also has a LSM 900 confocal unit with Airyscan2 (super-resolution, or live cell imaging). https://www.zeiss.com/microscopy/int/products/imaging-systems/celldiscovere… It should also allow automated workflows like overview imaging in widefield mode, locating objects of interest in x,y and z, followed by confocal imaging of these objects. The reason why we are interested in this is that they claim it is possible to image organoids at high resolution and in an automated workflow with this instrument. While there are limitations doing this with confocal microscopy and I would think this would be better done on a special light-sheet microscope, this demo could give us some answers. The positive aspect is that this is an all-in one box microscope, so it should be suitable for containment-level work (but not during the demo). I will also work for other samples like cells on coverslips, in dishes or on plates. Please let me know if you want to have your samples imaged during the demo which will take place in the SAF building at South Kensington. The time is limited (only 2.5 days) and will it takes 2 days to set up the microscope and ½ day to pack up. Best wishes Andreas ---------------------------------------------------- Dr Andreas Bruckbauer FILM facility manager Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 401 +44 (0)20 7594 2023 a.bruckbauer(a)imperial.ac.uk<mailto:a.bruckbauer@imperial.ac.uk> http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ http://www.imperial.ac.uk/people/a.bruckbauer ----------------------------------------------------

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[FILM-Users 01015] Immersion oil bottle missing from CF6
by Gaboriau, David C A 09 Mar '22

09 Mar '22

Dear all, We're missing the immersion oil bottle for the CF6 confocal, if someone borrowed it or took it by mistake please let me know! Many thanks, All the best, David ---------------------------------------------------- David Gaboriau, PhD, FRMS FILM Microscopy Specialist Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 408 +44 (0)20 7594 9793 d.gaboriau(a)imperial.ac.uk https://www.imperial.ac.uk/people/d.gaboriau http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ ----------------------------------------------------

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