- FILM-Users - imperial.ac.uk

[FILM-Users 01054] Request for antibodies
by Gaboriau, David C A 27 Oct '22

27 Oct '22

Dear Users, Please see below a request for antibodies, if you can help please reply to Antonia directly a.akoumianaki(a)imperial.ac.uk All the best, David ---------------------------------------------------- David Gaboriau, PhD, FRMS Interim FILM Facility Manager Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 408 +44 (0)20 7594 9793 d.gaboriau(a)imperial.ac.uk https://www.imperial.ac.uk/people/d.gaboriau http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ ---------------------------------------------------- Hi David, I hope all is well. Please, could you circulate the below request for a secondary antibody to FILM users for me? I would like to borrow 2ul of either Goat Anti-Mouse IgG (Alexa Fluor 488) or Goat Anti-Mouse IgG (Alexa Fluor 647) Thanking you in advance for your help. Best, Antonia

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[FILM-Users 01053] Wellcome Bioimaging Landscape Review
by Gaboriau, David C A 14 Oct '22

14 Oct '22

Dear Users, Please see below about a survey of the field of bioimaging, and the link to take the survey: https://www.research.net/r/55TLDXF?utm_campaign=2227811_October%20Research%… [https://www.research.net/collect/images/default_custom_meta_image.jpg]<https://www.research.net/r/55TLDXF?utm_campaign=2227811_October%20Research%…> Wellcome Bioimaging Landscape Review<https://www.research.net/r/55TLDXF?utm_campaign=2227811_October%20Research%…> Take this survey powered by surveymonkey.com. Create your own surveys for free. www.research.net Landscape review of barriers affecting progress in the field of Bioimaging Technopolis<https://www.technopolis-group.com/> is carrying out a global landscape review on behalf of Wellcome<https://wellcome.org/> to identify new key areas that have the potential to transform the field of bioimaging. The review will cover bioimaging methodologies, tools and technology development across the scales of life – from atoms all the way to humans – that are applicable to discovery research. An important component of the review is a stakeholder survey. Your participation in this survey will help us to understand: * What are the key emerging bioimaging technologies/methodologies? * What are the main barriers (with a specific focus on novel technology development) to progress and adoption of bioimaging technologies/methodologies? We welcome contributions from all researchers and scientists working in fields related to bioimaging, from academics and methodology/technology developers to infrastructure staff. To that end, please feel free to forward the survey link to colleagues, technical staff, postdoctoral researchers and postgraduate/doctoral students. Wellcome will use the outputs of this survey to inform its future strategy with regard to supporting bold, creative and high-quality research activities in the field of bioimaging. The survey will close on 31st October 2022. All the best, David ---------------------------------------------------- David Gaboriau, PhD, FRMS Interim FILM Facility Manager Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 408 +44 (0)20 7594 9793 d.gaboriau(a)imperial.ac.uk https://www.imperial.ac.uk/people/d.gaboriau http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ ----------------------------------------------------

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[FILM-Users 01052] Fw: Antibody request please
by Gaboriau, David C A 14 Oct '22

14 Oct '22

Dear Users, Please see below for an antibody request. If you can help Annabelle please reply to a.milner(a)imperial.ac.uk All the best, David ---------------------------------------------------- David Gaboriau, PhD, FRMS Interim FILM Facility Manager Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 408 +44 (0)20 7594 9793 d.gaboriau(a)imperial.ac.uk https://www.imperial.ac.uk/people/d.gaboriau http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ ---------------------------------------------------- ________________________________ From: Milner, Annabelle <a.milner(a)imperial.ac.uk> Sent: 14 October 2022 11:26 To: Gaboriau, David C A <d.gaboriau(a)imperial.ac.uk> Subject: Antibody request please Dear David I hope all is well. I'm currently running a pilot experiment looking at microvilli expression in Caco-2 cells. Please may you circulate the below request for a primary antibody (not raised in mouse) to FILM users for me? I'm looking to borrow 3-4 uL of either -Villin -Fimbrin/plastin 1 -ESPN -Ezrin Or any other antibody that would be recommended for looking at microvilli Many thanks! Annabelle

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[FILM-Users 01051] FW: Bcl-2 antibody
by Rothery, Stephen M 04 Oct '22

04 Oct '22

Dear All If anyone can help Laura - see below, please contact her directly l.nicastro19(a)imperial.ac.uk<mailto:l.nicastro19@imperial.ac.uk> (Nicastro, Laura ) DO NOT REPLY TO THIS E_MAIL Bw Steve Hi Steve, I am looking for a small aliquot (3-5 ul) of the antibody Bcl-2. Would you mind circulating this email in the building to see if anyone has some to spare? Thank you very much in advance! Best, Laura

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[FILM-Users 01050] Fwd: Workshop ‘Automated Microscopy for Everyone’ by Acquifer - Automated Zebrafish Imaging - Please register for Demonstration and Hands-on Session
by Gaboriau, David C A 04 Oct '22

04 Oct '22

Dear FILM users, The Pantazis lab in Bioengineering is organising an instrument demo of the ACQUIFER Imaging Machine in Bessemer building at the end of October, please see below for details of the seminar and workshop. All the best, David Begin forwarded message: From: "Pantazis, Periklis L" <p.pantazis(a)imperial.ac.uk<mailto:p.pantazis@imperial.ac.uk>> Subject: Workshop ‘Automated Microscopy for Everyone’ by Acquifer - Automated Zebrafish Imaging - Please register for Demonstration and Hands-on Session Date: 1 October 2022 at 00:34:41 BST Dear London Zebrafish PI Community, dear London Zebrafish Club organising committee, I hope you are doing fine. The Pantazis Lab will be hosting a Workshop ‘Automated Microscopy for Everyone’ by Acquifer at the Department of Bioengineering of Imperial College London (Bessemer Building – see attached map). Acquifer will present the ACQUIFER Imaging Machine, an automated microscope following a unique opto-mechanical design rendering it ideal for imaging non-adherent, motion sensitive or large specimens. The Programme is as follows: * Online Seminar: 19th of October 2022, 14:00 to 16:00 * Demonstration and Hands-on Sessions: 25th to 27th of October, 2022 3-4 hour slots per small group (4 to 5 participants) It will be great if you can distribute the attached Acquifer Workshop pdf (includes a link for registration)among your lab members/the zebrafish community at your earliest convenience. I am happy to address any further questions! We will be excited to welcome many users at our place! Best wishes and take care, Laki __ Periklis (Laki) Pantazis PhD Imperial College London | Department of Bioengineering | South Kensington Campus | London SW7 2AZ Laboratory of Advanced Optical Precision Imaging | Lab B104 | Office RSM314 Tel: +44 (0)20 7594 6367 | Web: http://www.pantazis-lab.org<http://www.pantazis-lab.org/> | Email: p.pantazis(a)imperial.ac.uk <mailto:p.pantazis@imperial.ac.uk%C2%A0>

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[FILM-Users 01049] FW: Job advertisement
by Rothery, Stephen M 26 Sep '22

26 Sep '22

Dear All The Cimini lab at the Broad Institute has a post-doc position open https://docs.google.com/document/d/19DPvackYH6QfP9JUoVhq2l6iYNTAtMowwerrkPZ… [https://lh6.googleusercontent.com/PRtO3OlzP5iYWLKgtgqhlJ7Srh6cARj-FRkau6JP-…]<https://docs.google.com/document/d/19DPvackYH6QfP9JUoVhq2l6iYNTAtMowwerrkPZ…> Image assay development postdoc job description<https://docs.google.com/document/d/19DPvackYH6QfP9JUoVhq2l6iYNTAtMowwerrkPZ…> Postdoctoral Training Program in Bioimage Analysis Broad Institute of MIT and Harvard, Cambridge, MA Impact the discovery of new disease therapies in the Cimini laboratory at the Imaging Platform Join our vibrant, world-class, nonprofit biomedical research center, the Broad Institute of MIT ... docs.google.com Bw Steve

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[FILM-Users 01048] Fw: FILM Reagent Request (AlexaFluor-568 anti-human)
by Gaboriau, David C A 20 Sep '22

20 Sep '22

Dear FILM Users, Please see a request for antibody, and reply to Alex directly if you can help, not to this message. Hi All, does anyone have a small aliquot of Alexafluor-568 anti-human that I would be able to borrow for some imaging experiments this week? Any help would be much appreciated! a.bailey18(a)imperial.ac.uk All the best, David ---------------------------------------------------- David Gaboriau, PhD, FRMS Interim FILM Facility Manager Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 408 +44 (0)20 7594 9793 d.gaboriau(a)imperial.ac.uk https://www.imperial.ac.uk/people/d.gaboriau http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ ---------------------------------------------------- ________________________________ From: Bailey, Alexander <a.bailey18(a)imperial.ac.uk> Sent: 20 September 2022 14:20 To: Rothery, Stephen M <s.rothery(a)imperial.ac.uk>; Gaboriau, David C A <d.gaboriau(a)imperial.ac.uk> Subject: FILM Reagent Request (AlexaFluor-568 anti-human) Hi David and Stephen, I hope all is well - would you please be able to circulate the below request for a secondary antibody to FILM users? Hi All, does anyone have a small aliquot of Alexafluor-568 anti-human that I would be able to borrow for some imaging experiments this week? Any help would be much appreciated! Thanks, and Best Wishes, Alex

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[FILM-Users 01047] TODAY - FILM Club - AKOYA Biosciences - multiplex imaging
by Gaboriau, David C A 20 Sep '22

20 Sep '22

Dear FILM Users, A reminder of today's FILM Club talk, at 12pm. We are pleased to welcome Dr Adam Dickinson from Akoya Biosciences to the FILM Club to talk about multiplex imaging and high-plex spatial phenotyping. Tuesday 20th September 2022 Midday-1pm Please see the attached posters and the Teams link below. Looking forward to seeing you there, All the best, David ---------------------------------------------------- David Gaboriau, PhD, FRMS Interim FILM Facility Manager Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 408 +44 (0)20 7594 9793 d.gaboriau(a)imperial.ac.uk<mailto:d.gaboriau@imperial.ac.uk> https://www.imperial.ac.uk/people/d.gaboriau http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ ---------------------------------------------------- ___________________________________________________________ Microsoft Teams meeting Join on your computer or mobile app Click here to join the meeting<https://teams.microsoft.com/l/meetup-join/19%3ameeting_OTc4OWEzY2EtODQ4OS00…> Meeting ID: 396 361 024 461 Passcode: MCkGpM Download Teams<https://www.microsoft.com/en-us/microsoft-teams/download-app> | Join on the web<https://www.microsoft.com/microsoft-teams/join-a-meeting> All the best, David ---------------------------------------------------- David Gaboriau, PhD, FRMS Interim FILM Facility Manager Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 408 +44 (0)20 7594 9793 d.gaboriau(a)imperial.ac.uk https://www.imperial.ac.uk/people/d.gaboriau http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ ----------------------------------------------------

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[FILM-Users 01046] FILM Club - Akoya Biosciences - Tuesday 20th September
by Gaboriau, David C A 15 Sep '22

15 Sep '22

Dear FILM Users, We are pleased to welcome Dr Adam Dickinson from Akoya Biosciences to the FILM Club to talk about multiplex imaging and high-plex spatial phenotyping. Tuesday 20th September 2022 Midday-1pm Please see the attached posters and the Teams link below. Looking forward to seeing you there, All the best, David ---------------------------------------------------- David Gaboriau, PhD, FRMS Interim FILM Facility Manager Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 408 +44 (0)20 7594 9793 d.gaboriau(a)imperial.ac.uk<mailto:d.gaboriau@imperial.ac.uk> https://www.imperial.ac.uk/people/d.gaboriau http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ ---------------------------------------------------- ___________________________________________________________ Microsoft Teams meeting Join on your computer or mobile app Click here to join the meeting<https://teams.microsoft.com/l/meetup-join/19%3ameeting_OTc4OWEzY2EtODQ4OS00…> Meeting ID: 396 361 024 461 Passcode: MCkGpM Download Teams<https://www.microsoft.com/en-us/microsoft-teams/download-app> | Join on the web<https://www.microsoft.com/microsoft-teams/join-a-meeting> [https://ci5.googleusercontent.com/proxy/quhhKXrbI7O8O8adiVf3hbHzgsyk2dvyF6y…] Learn more<https://aka.ms/JoinTeamsMeeting> | Meeting options<https://teams.microsoft.com/meetingOptions/?organizerId=113c5bee-3703-4299-…> | Legal<https://www.imperial.ac.uk/media/imperial-college/administration-and-suppor…> ________________________________________________________________________________

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[FILM-Users 01045] Leaving FILM
by Bruckbauer, Andreas 05 Sep '22

05 Sep '22

Dear FILM-users, I have very much enjoyed working with you, especially because of the nice people at Imperial and the help I received from my fantastic colleagues. After six years, it is now time for me to move on and I will be leaving Imperial at the end of this week to start a new position at the University of Cambridge which is much closer to where I live (No more commuting on the train!). I am sure that FILM will have an excellent new manager soon and the good work of the facility will continue with minimum disruption. Meanwhile David Gaboriau will serve as an interim facility manager until a new manager is appointed. I will bring some doughnuts to the 3rd floor meeting area in SAF at Thursday September 8th starting from 15:30 and, if you are around, it would be nice to say goodbye. I wish everyone good luck with your projects as well as happy and successful microscopy experiments! Best wishes Andreas ---------------------------------------------------- Dr Andreas Bruckbauer FILM facility manager Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 401 +44 (0)20 7594 2023 a.bruckbauer(a)imperial.ac.uk<mailto:a.bruckbauer@imperial.ac.uk> http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ http://www.imperial.ac.uk/people/a.bruckbauer ----------------------------------------------------

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