- FILM-Users - imperial.ac.uk

[FILM-Users 00207] Fwd: anyone anti-mCherry antibody for use in western blot?
by Martin Spitaler 11 May '12

11 May '12

Can anybody help? Martin -------- Original Message -------- Subject: anyone anti-mCherry antibody for use in western blot? Date: Thu, 10 May 2012 11:16:46 +0100 From: Semplici, Francesca <f.semplici(a)imperial.ac.uk> To: Spitaler, Martin <m.spitaler(a)imperial.ac.uk> Dear Martin, I am looking for an anti-mCherry antibody that I could borrow (a few microliters for a western blot) if anyone has some. Could you please be so kind to circulate my request to FILM users? Thank you so much indeed. Best wishes, Francesca

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[FILM-Users 00206] Black cardigan
by Rothery, Stephen M 11 May '12

11 May '12

Hi all A small black cardigan (ladies medium) has been left in the widefield room. It has been there for a few weeks/months now If anyone has missed it, it is now hanging on my desk (403). Please come and collect Bw Steve FILM - Facility for Imaging by Light Microscopy ************************************************************** Steve Rothery FILM Technician Sir Alexander Fleming Building, desk 403 Imperial College London / South Kensington Exhibition Road London SW7 2AZ Tel. +44-(0)207 594 9793 E-mail s.rothery(a)imperial.ac.uk<mailto:s.rothery@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility **************************************************************

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[FILM-Users 00205] FILM server down
by Martin Spitaler 10 May '12

10 May '12

Dear microscopists, unfortunately the FILM server is down at the moment, ICT have been contacted and will hopefully get it back up and running again soon. Sorry for the troubles, good imaging, Martin -- ------------------------------------------------------------------------ *Martin Spitaler, PhD* *FILM - Facility for Imaging by Light Microscopy* - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk <mailto:m.spitaler@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

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[FILM-Users 00204] FILM server down
by Martin Spitaler 10 May '12

10 May '12

Dear microscopists, unfortunately the FILM server is down at the moment, ICT has been contacted and will hopefully get it back up and running again soon. Sorry for the troubles, good imaging, Martin -- ------------------------------------------------------------------------ *Martin Spitaler, PhD* *FILM - Facility for Imaging by Light Microscopy* - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk <mailto:m.spitaler@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

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[FILM-Users 00203] FILM Microscopy Day 2012
by Martin Spitaler 10 May '12

10 May '12

Dear microscopists, on FRIDAY 29 JUNE we will have the FILM MICROSCOPY DAY 2012 - as in previous years, a day full of talks on all aspects of light microscopy, from the very basics to very advanced technologies (in vivo imaging, super-resolution, non-linear techniques). The presentations will be by FILM staff (Steve, Mark and myself) supported by two experts in their field, VALENTINA CAORSI (NHLI) on non-linear fluorescence techniques (fluorescence lifetime imaging and its various applications) and LUIS PIZARRO (COMPUTING) on image analysis. We also have a special novelty: To make people aware what's going on around them and stimulate new collaborations, we will have a MICROSCOPY MATCH MAKER, where you FILM users are all invited to present your exciting research projects in a short snapshot oral presentation and / or a poster. Oral presentations are strictly limited to a MAXIMUM OF 2 MINUTES / 2 POWERPOINT SLIDES - longer discussions will be possible at the posters over lunchtime. An excellent opportunity to initiate new collaborations, don't miss out - PIs, please encourage your students and postdocs to participate. Please REGISTER BY FRIDAY 1 JUNE by email to Ruth O'Donnell <r.odonnell(a)imperial.ac.uk>, including your MATCHMAKER OPTIONS (presentation, poster, none). >>PROGRAMME<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< 10.00- 10.10 Welcome by Prof Tony Magee, head of FILM GENERAL MICROSCOPY TECHNIQUES 10.10-10.55 Basics of microscopy - magnification, properties of light, resolution, transmitted light contrast (PH, DIC, darkfield), epifluorescence, confocal microscopy (Martin Spitaler, FILM) 10.55-11.40 Live imaging techniques - Multiphoton, live imaging and second harmonic generation (SHG) (Mark Scott, FILM) 11.40-12.00 coffee & tea break 12.000-13.00 MICROSCOPY MATCHMAKER: FILM users giving a snapshot of their work in 2-minute presentations 13.00-14.15 Poster session with sandwich lunch (kindly sponsored by CARL ZEISS MICROSCOPY) SPECIAL TECHNIQUES 14.15-15.00 Fluorophores as sensors - colocalisation, fluorescence lifetime (FLIM) and resonant energy transfer (FRET) (Valentina Caorsi, NHLI) 15.00-15.45 Super-resolution microscopy - TIRF, PALM, STORM (Steve Rothery, FILM) 15.45-16.05 coffee & tea break UNDERSTANDING IMAGES 16.05-16.50 Understanding and visualisation of image data: data formats, pixel and metadata, image visualisation and presentation, OMERO database (Martin Spitaler, FILM) 16.50-17.35 Image analysis: noise removal, deconvolution, segmentation, tracking, registration (Luis Pizarro, Computing) 17.35-17.40 Wrap-up, feedback, outlook For more and up-to-date information, please check <http://www3.imperial.ac.uk/portal/page/portallive/imagingfacility/news>. A flyer is also available <https://workspace.imperial.ac.uk/imagingfacility/Public/FILM%20Microcopy%20…> See you all there. Good imaging, Martin -- ------------------------------------------------------------------------ *Martin Spitaler, PhD* *FILM - Facility for Imaging by Light Microscopy* - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk <mailto:m.spitaler@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

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[FILM-Users 00202] Fwd: Definiens Symposium 2012 in München
by Martin Spitaler 04 May '12

04 May '12

...forwarded, for your information; should be an interesting meeting, Martin -------- Original Message -------- Subject: Definiens Symposium 2012 in München Date: Fri, 4 May 2012 17:47:12 +0200 From: Zimmermann, Johannes <jzimmermann(a)definiens.com> To: <m.spitaler(a)imperial.ac.uk> CC: Huber, Monika <mhuber(a)definiens.com> Hello Martin, I regret very much not seeing you in Munich at the Symposium in June (14-16). Maybe the event (especially the workshops) would be interesting for people in your group as well. As discussed, please find below some additional information: Symposium Page: http://www.definiens.com/community/symposium.html Program: http://www.definiens.com/community/symposium/symposium-2012-program.html Workshops: http://www.definiens.com/community/symposium/symposium-2012-workshops.html Registration: http://info.definiens.com/2012Symposium_RegistrationPage.html Please let me know if you have further questions. Kind regards Johannes cid:image001.jpg@01CD02D1.DBAE0E10 ------------------------------------------------------------------------ *Dr. Johannes Zimmermann* Director Business Development Life Sciences Definiens AG Bernhard-Wicki-Str. 5 80636 München Germany Tel. +49 (89) 231180 32 Fax. +49 (89) 231180 90 Mob. +49 (162) 2665580 jzimmermann(a)definiens.com <mailto:jzimmermann@definiens.com> http://www.definiens.com <http://www.definiens.com/> cid:image002.png@01CD02D1.DBAE0E10 <http://www.definiens.com/community/symposium.html> ------------------------------------------------------------------------ *Deeper Insights - Faster Results - Better Decisions* ------------------------------------------------------------------------ Sitz der Gesellschaft/Registered Office: Munich, Germany; Vorstand/Executive Board: Thomas Heydler (Vorsitzender/CEO), Dr. Markus Rinecker; Vorsitzender des Aufsichtsrats/Chairman of the Supervisory Board: Friedrich Bornikoel Registergericht/Commercial Register München HRB 133088 This e-mail may contain trade secrets or privileged, undisclosed, or otherwise confidential information. If you have received this e-mail in error, you are hereby notified that any review, copying, or distribution of it is strictly prohibited. Please inform us immediately and destroy the original transmittal. Thank you for your cooperation. ------------------------------------------------------------------------ /IMPORTANT: The contents of this email and any attachments are confidential. They are intended for the named recipient(s) only. If you have received this email in error, please notify the system manager or the sender immediately and do not disclose the contents to anyone or make copies thereof. /**** eSafe scanned this email for viruses, vandals, and malicious content. **** ------------------------------------------------------------------------

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[FILM-Users 00201] Fwd: CONFOCALMICROSCOPY: Leica web-seminar : , , Live Cell Imaging: Cell-Cell Interactions"
by Martin Spitaler 03 May '12

03 May '12

...forwarded, for your information, Martin -------- Original Message -------- Subject: CONFOCALMICROSCOPY Index - 1 May 2012 to 2 May 2012 (#2012-105) Date: Thu, 3 May 2012 00:06:28 -0500 From: CONFOCALMICROSCOPY automatic digest system <LISTSERV(a)LISTS.UMN.EDU> To: <CONFOCALMICROSCOPY(a)LISTS.UMN.EDU> CONFOCALMICROSCOPY Index - 1 May 2012 to 2 May 2012 (#2012-105) **Commercial Posting** Leica has organized a web-seminar in May that may be of interest to you: Live Cell Imaging: Cell-Cell Interactions Date: 24th May 2012 Time: 12:00pm ET Duration: 60 minutes http://mediazone.brighttalk.com/event/ReedElsevier/95e1533eb1-6086-intro [Please note that if the url above gets cut onto the next line, you will need to merge it] Speakers: Jeff Lichtman Professor of Molecular and Cellular Biology, Harvard University Quentin Sattentau Professor of Immunology, University of Oxford Dr. Ellen Robey Professor of Immunology and Pathogenesis, University of California, Berkeley In this interactive webinar, you will hear from renowned scientists who use live- cell imaging to study not only how cell-cell communication facilitates neuronal and immune cell interactions, but also how these modes of communication can be altered in the context of infection to promote pathogen transmission. Very best regards, Lon Nelson Director of Marketing Management Leica Microsystems, Inc. Living up to Life (847) 317-7218 (800) 248-0123 lon.nelson(a)leica-microsystems.com http://www.leica-microsystems.com/

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[FILM-Users 00200] FW: Sign up Today for the Latest Science Webinar: Deciphering the Cell
by Martin Spitaler 30 Apr '12

30 Apr '12

...forwarded, for your information, Martin -------- Original Message -------- Subject: FW: Sign up Today for the Latest Science Webinar: Deciphering the Cell Date: Sat, 28 Apr 2012 08:01:19 +0100 From: Magee, Tony <t.magee(a)imperial.ac.uk> FYI. Best, Tony From: info(a)aaas-science.org [mailto:info@aaas-science.org] On Behalf Of Science Webinars Sent: 27 April 2012 10:53 To: Magee, Tony Subject: Sign up Today for the Latest Science Webinar: Deciphering the Cell View on mobile or on web page Science Webinar Series New complimentary webinar from Science: Deciphering the Cell: Applying Super Resolution Imaging and HCA to Reveal Cellular Processes You are invited to view our panel of experts on Thursday, May 17th, in this live, online educational seminar. For more information and complimentary registration visit: webinar.sciencemag.org Date: Thursday, May 17th, 2012 Time: 12 noon Eastern, 9 a.m. Pacific, 4 p.m. GMT, 5 p.m. UK Duration: 1 hour Submit your questions LIVE to the experts during the webinar! The latest imaging technologies are enabling new discoveries by helping scientists visualize the detailed inner workings of the cell. Super-resolution microscopy is now providing resolution approaching that of some electron microscopes, enabling scientists to literally see previously unimaginable molecular-level detailâeven in live cells. At the same time, high content analysis can automatically image and analyze >100 parameters per cell at high throughput, removing human subjectivity and speeding data acquisition. Using these technologies together provides a powerful approach, enabling scientists to answer complex questions previously too difficult or time consuming to undertake using more traditional techniques. In this webinar our key opinion leaders will share their views on how these synergistic technologies are advancing their research. Webinar viewers will: Learn how experts are combining super-resolution microscopy with HCA in their research Discover the advantages and challenges in using these techniques together Obtain insight into handling rapid data acquisition, analysis, and presentation Have their questions answered live by our in-studio panel. Speakers: Laurence Pelletier, Ph.D. Lunenfield Research Institute Toronto, Canada Nick Thomas, Ph.D. GE Healthcare Cardiff, Wales Register at: webinar.sciencemag.org Produced by the Science /AAAS Custom Publishing Office and sponsored by GE Healthcare and API. Manage Your E-mail Subscription Preferences | Unsubscribe Need help? Contact memuser(a)aaas.org. This e-mail was sent to: T.MAGEE(a)IMPERIAL.AC.UK AAAS / Science | 1200 New York Avenue NW | Washington, DC 20005 | U.S.A. +1 202-326-6417 | memuser(a)aaas.org | Privacy Policy

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[FILM-Users 00199] Fwd: That's new: ESMI PhD AWARD
by Martin Spitaler 27 Apr '12

27 Apr '12

...forwarded, for your information, Martin ================================================================ -------- Original Message -------- Subject: That's new: ESMI PhD AWARD Date: Fri, 27 Apr 2012 09:31:44 +0200 From: ESMI <office(a)e-smi.eu> To: Imaging Community <Mi_community(a)lists.nf.mpg.de> Dear Colleagues, We would like to invite all young scientists working in the broad field of Molecular Imaging to apply for an *ESMI PhD Award* for excellent PhD thesis. The Societies’ PhD Award for excellent PhD thesis is offered for the purpose of encouraging and rewarding outstanding research conducted by our young members. *Next submission deadline: 30 June 2012* Read more about the prize, eligibility criteria, and how to apply at www.e-smi.eu/index.php?id=2451 <http://www.e-smi.eu/index.php?id=2451> Feel free to forward this to anyone who might be interested and do not hesitate to contact me for any further information. Looking forward to your application and to learn more about your excellent work. Best regards, Doris on behalf of the ESMI PhD Award Committee Annemie van der Linden - Antwerp, Belgium Fabian Kiessling - Aachen, Germany Michal Neeman - Rehovot, Israel ** ** *Doris Kracht* ESMI – European Society for Molecular Imaging** www.e-smi.eu <http://www.e-smi.eu/>__ office(a)e-smi.eu <mailto:office@e-smi.eu>__ phone ++49 221 28206832 fax ++49 221 28206868 Eupener Str. 57-59 50933 Köln *Molecular****Imaging****Meetings****2012*** *2012, April 15-20 TOPIM <http://www.topim.eu/>Processing Biomedical Images Les Houches, France* *2012, Sep. 5-8 World Molecular Imaging Congress WMIC and 7^th EMIM <http://www.e-smi.eu/index.php?id=2040> Dublin, Ireland * *2012, Sep. 10-14 workshop on Quantitative Image Analysis and Application Specific Imaging - PRIMA IV <http://www.e-smi.eu/index.php?id=2446> Dublin, Ireland *

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[FILM-Users 00198] Fwd: adenoviral lifeact-RFP
by Martin Spitaler 27 Apr '12

27 Apr '12

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