- FILM-Users - imperial.ac.uk

[FILM-Users 00176] food in microscope rooms
by Martin Spitaler 20 Mar '12

20 Mar '12

Dear microscopists, it seems it's necessary again to remind everybody that eating and drinking is NOT allowed in any FILM microscope room! Recently it happened more often again that coffee cups and coke cans appeared in the rooms. All our microscopes are lab space, and harmful chemicals (fixatives, nuclear stainings, oncogene transfections, ...) are regularly used in sample preparation. As in any lab, traces of them will accumulate on the microscopes, computer keyboards etc. There is a real reason for the rule; plus, it can get us into serious troubles with the health and safety inspectors. This should also remind you of obvious lab behaviour, that it's a good idea to wash your hands after leaving the rooms, and that it's not a good idea to bite your nails either, no matter how exciting (or frustrating) the experiment. Thanks for your cooperation, good imaging, Martin -- ------------------------------------------------------------------------ *Martin Spitaler, PhD* *FILM - Facility for Imaging by Light Microscopy* - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk <mailto:m.spitaler@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

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[FILM-Users 00175] webinar on super-resolution
by Martin Spitaler 08 Mar '12

08 Mar '12

...forwarded, for your information, Martin WEBINAR: BioTechniques / Leica Microsystems: "Beyond the Diffraction Limit: Advances and Applications of Super-Resolution Imaging" Wednesday, March 28, 2012 1 PM ET / 10 AM PT / 5 PM GMT https://event.on24.com/eventRegistration/EventLobbyServlet?target=registrat… -- ------------------------------------------------------------------------ *Martin Spitaler, PhD* *FILM - Facility for Imaging by Light Microscopy* - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk <mailto:m.spitaler@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

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[FILM-Users 00174] Fwd: CFP and E2-Crimson fluorescent proteins
by Martin Spitaler 28 Feb '12

28 Feb '12

...forwarded on behalf of Elisa, can anybody help? Please reply to Elisa directly and NOT to the mailing list, Martin -------- Original Message -------- Subject: CFP and E2-Crimson fluorescent proteins Date: Tue, 28 Feb 2012 09:14:59 +0000 From: Belian, Elisa <e.belian09(a)imperial.ac.uk> To: Martin Spitaler <m.spitaler(a)imperial.ac.uk> Dear Martin, I was wondering if you know anyone who has plasmids expressing CFP and/ or E2-Crimson and could give me some DNA or bacterial stocks of those plasmids. I want to express those proteins in mammalian cells, so an expression vector would be ideal, but otherwise any vector will do. Thank you for circulating the email. Best, Elisa *Elisa Belian* * * Cardiac Myogenesis, Death and Regeneration National Heart and Lung Institute Faculty of Medicine Imperial College London 2nd Floor Sir Alexander Fleming Building Exhibition Road SW7 2AZ London United Kingdom E-mail: e.belian09(a)imperial.ac.uk <mailto:e.belian09@imperial.ac.uk>

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[FILM-Users 00173] TODAY: FILM Club on sample fixation
by Martin Spitaler 24 Feb '12

24 Feb '12

TODAY: Dear microscopists, in this Friday's FILM Club we will try something new: Some kind of technical mini-workshop on sample preparation and fixed versus live imaging. This will be based on the following recent publication, which I will summarise in the FILM Club: Immunolabeling artifacts and the need for live-cell imaging U Schnell, F Dijk, KA Sjollema & BNG Giepmans Nature Methods 9,152158(2012) <http://www.nature.com/nmeth/journal/v9/n2/full/nmeth.1855.html> The FILM Club will be held this Friday 24 Feb 2012 at 1pm in SAFB-120 (Fleming Building, South Kensington) See you there, good imaging, Martin -- ------------------------------------------------------------------------ *Martin Spitaler, PhD* *FILM - Facility for Imaging by Light Microscopy* - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk <mailto:m.spitaler@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

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[FILM-Users 00172] Fwd: FW: Perkin Elmer OPERETTA SYSTEM - please circulate
by Martin Spitaler 22 Feb '12

22 Feb '12

...forwarded for your information, Martin -------- Original Message -------- Subject: FW: Perkin Elmer OPERETTA SYSTEM - please circulate Date: Tue, 21 Feb 2012 15:57:10 +0000 From: Hipwell, Mandy A <m.hipwell(a)imperial.ac.uk> *From:*Ettorre, Anna *Sent:* 21 February 2012 15:40 *To:* Lee, Matt J; Boyton, Rosemary J; Altmann, Danny M; Friedland, Jon S; Muraro, Paolo A; Wojciak-Stothard, Beata; Karadimitris, Anastasios; Franzoso, Guido; Archanco, Marta; Mazarakis, Nicholas D B.Sc, Ph.D, FSB *Cc:* Jones, Amanda; 'kate.harper(a)perkinelmer.com'; Konitsiotis, Antonios D; Elkington, Paul T G; Purbhoo, Marco *Subject:* Perkin Elmer OPERETTA SYSTEM - please circulate Dear colleagues, Perkin Elmer has agreed to bring their High Content Imaging OPERETTA SYSTEM (http://www.perkinelmer.com/pages/020/cellularimaging/products/operetta.xhtml ) for a week demo to our BRC Facility at Hammersmith Campus (http://www1.imperial.ac.uk/departmentofmedicine/about/brc_imaging_and_facs_… ). The week demo will start on March 6^th until March 8^th . For those who are interested in having only a general overview of this High Content Imaging system, we will host a seminar on March 6^th , Wolfson Seminar Centre, seminar room 4, h: 11-12. Anybody who wants to try using his/her own cells is welcome to come. You can book a slot either by contacting directly Amanda Jones (*amanda.jones(a)perkinelmer.com <mailto:amanda.jones@perkinelmer.com>*) or by emailing myself. If you would like to have more info, please do not hesitate to contact me. Hope to meet you there. Best regards, Anna Ettorre Anna Ettorre, Ph.D. Research Fellow Faculty of Medicine Department of Medicine Section of Infectious Diseases and Immunity Commonwealth Building Hammersmith Campus Imperial College London DuCane Road London W12 0NN Tel: +44 (0)208 383 1284 Fax: +44 (0)20 8383 3394 e-mail: a.ettorre(a)imperial.ac.uk <mailto:a.ettorre@imperial.ac.uk>

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[FILM-Users 00171] FILM Club Fri 24 Feb 2011: sample preparation
by Martin Spitaler 22 Feb '12

22 Feb '12

Dear microscopists, in this Friday's FILM Club we will try something new: Some kind of technical mini-workshop on sample preparation and fixed versus live imaging. This will be based on the following recent publication, which I will summarise in the FILM Club: Immunolabeling artifacts and the need for live-cell imaging U Schnell, F Dijk, KA Sjollema & BNG Giepmans Nature Methods 9,152–158(2012) <http://www.nature.com/nmeth/journal/v9/n2/full/nmeth.1855.html> The FILM Club will be held this Friday 24 Feb 2012 at 1pm in SAFB-120 (Fleming Building, South Kensington) See you there, good imaging, Martin -- ------------------------------------------------------------------------ *Martin Spitaler, PhD* *FILM - Facility for Imaging by Light Microscopy* - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk <mailto:m.spitaler@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

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[FILM-Users 00169] OMERO and Definiens server down
by Martin Spitaler 16 Feb '12

16 Feb '12

Dear microscopists, unfortunately the server hosting Omero and Definiens is down at the moment, some unexpected technical fault. BSS are working on it, hopefully restarting will do the job and it will be back up soon again. Good imaging, Martin -- ------------------------------------------------------------------------ *Martin Spitaler, PhD* *FILM - Facility for Imaging by Light Microscopy* - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk <mailto:m.spitaler@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

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[FILM-Users 00168] Fwd: Operetta demo and HCS workshop
by Martin Spitaler 15 Feb '12

15 Feb '12

...forwarded, for your information, Martin -------- Original Message -------- Subject: Operetta demo and HCS workshop Date: Wed, 15 Feb 2012 15:08:44 +0000 From: Konitsiotis, Antonios D <antonios.konitsiotis05(a)imperial.ac.uk> To: Spitaler, Martin <m.spitaler(a)imperial.ac.uk> Hi Martin, Can you please forward this to the FILM group? Thanks!! Following the visit from Perkin-Elmer in November we have been organising a date and place to demo the High content screening capabilities of the Operetta machine, as well as the Columbus software. With the help of Anna Ettorre from the BRC imaging and FACs facility we now have the dates and place sorted and I am collecting names for people from the South Kensington groups that would like to demo the machine and see if it is suitable for the work they are doing. The details of the workshop are as follows: Date: *6-8th March 2012* Place: *Imperial College Hammersmith Campus* Tuesday 6th March: *11:00 – 12:00 HCS Seminar (open to all)* @ The Wolfson Centre, Seminar Room 4, Hammersmith Campus. What is High Contents Screening? with Hans Pirard from PerkinElmer HCS instruments for everyone: Opera & Operetta/Harmony & Phenologic/Columbus Image Data Management. Applications with HCS *HCS Workshops* @ BRC Imaging and FACS Facility, Room 8.N.4, 8th Floor, Infectious Diseases and Immunity, Commonwealth Building, Hammersmith Campus *Tuesday 6th March*: *14:00 – 16:00 Pre-Bookable HCS Workshop * *Wednesday 7th March*: *10:00 – 13:00 Pre-Bookable HCS Workshop* *14:00 – 16:00* *Pre-Bookable HCS Workshop* *Thursday 8th March*: *10:00 – 13:00 - Pre-Bookable HCS Workshop* If anyone is interested in booking a slot to demo the machine please e-mail me (*antonios.konitsiotis05(a)imperial.ac.uk*) or e-mail (*amanda.jones(a)perkinelmer.com)*with the date and slot you prefer. All the best, Antonio */Antonio Konitsiotis/* Post-Doctoral Research Associate National Heart and Lung Institute, Division of Molecular Medicine, Sir Alexander Flemming Building, Imperial College South Kensington Campus London, SW7 2AZ UK

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[FILM-Users 00167] Fwd: FW: [High-Throughput-Technology-Community] High Throughput/High Content Technology Community
by Martin Spitaler 10 Feb '12

10 Feb '12

...forwarded, for your information, Martin -------- Original Message -------- Subject: FW: [High-Throughput-Technology-Community] High Throughput/High Content Technology Community Date: Thu, 9 Feb 2012 08:03:08 +0000 From: Sharp, Emma L <e.sharp(a)imperial.ac.uk> Dear All, Please see the note below from Rob Fenton for your information. Best wishes, Emma *From:*high-throughput-technology-community-bounces@imperial.ac.uk <mailto:high-throughput-technology-community-bounces@imperial.ac.uk> [mailto:high-throughput-technology-community-bounces@imperial.ac.uk] <mailto:[mailto:high-throughput-technology-community-bounces@imperial.ac.uk]> *On Behalf Of *Fenton, Rob J *Sent:* 08 February 2012 19:26 *To:* high-throughput-technology-community; Trim, Kimberley; Wilson, Rebecca K; Sri-Pathmanathan, Anusha A; Ip, Molly M L; Kerr, Colin; O' Neill, Anne; Oakeshott, John J; Reilly, John B; Spargo, Sheena; Tebbutt, Andrew C; Venables, Tim; Anderson, Diana R; Boyce, Eileen S; Evans, Pat M; Gayer, Gosia; Girard, Francis; Gray, Sandy E; Jones, Linda D; Jones, Richard L; Lyle, Karen R; Nurboja, Sandrine A C; Skene, Barbara; Tortelli, Maria A *Subject:* [High-Throughput-Technology-Community] High Throughput/High Content Technology Community Dear Colleague, Please could you cascade the following announcement to your respective departments. The meeting is next month so I appreciate your help with this. Best wishes Rob ..................................................................................................................................................................................................................................................................................................... *_FIRST ANNOUNCEMENT _** *High Throughput.jpg *INSTITUTE OF BIOMEDICAL ENGINEERING * *High Throughput/High Content Technology Community * *Inaugural Symposium * Wednesday 21st March 2012 12:00 – 18:00 Lecture Theatre G34, Sir Alexander Fleming Building, South Kensington Campus, Imperial College London _map <https://workspace.imperial.ac.uk/campusinfo/public/sthkencampus.pdf>_ *PLEASE PUT A NOTE IN YOUR DIARIES * In conjunction with the Francis Crick Institute, the Institute of Biomedical Engineering is pleased to announce the inaugural symposium of the High Throughput/High Content Technology Community. Please join us for a stimulating multidisciplinary programme of presentations. All welcome. This will be an excellent opportunity to meet and exchange ideas with colleagues from across the College. *Symposium * *Coffee and Registration at 12 noon * *Programme from 12:30 – 18:00 (see attached)* *Drinks Reception 18:00 * *To **register**for the symposium please visit: * *http://lwf-dev.cc.ic.ac.uk/bioeng/networks/event.php?event_id=6 * *(Please note that the above link only works on Imperial PCs. If you have problems registering, please use the registration forms on the Networks Membership page <http://www3.imperial.ac.uk/biomedeng/networks> ) or those attached.* *Dr Rob Fenton* *Consultant Research Advisor* *Institute of Biomedical Engineering* *RSM 4.23* *Royal School of Mines* *South Kensington Campus*** *Imperial College* *London SW7 2AZ* *Tel: 020 759 41500* *Mob: 07872 850122* *http://www3.imperial.ac.uk/people/r.fenton*

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[FILM-Users 00166] Fwd: [CISBIC-RA] [cisbio] FW: PDRA in image analysis
by Martin Spitaler 06 Feb '12

06 Feb '12

...forwarded for your information, Martin -------- Original Message -------- Subject: [CISBIC-RA] [cisbio] FW: PDRA in image analysis Date: Sun, 5 Feb 2012 14:39:20 +0000 From: Endres, Robert G <r.endres(a)imperial.ac.uk> To: cisbio <cisbio(a)imperial.ac.uk> FYI - open postdoc position in image analysis at Cambridge. Best, Robert ---------------------------------------------------------------- Dr. Robert Endres Senior Lecturer in Systems Biology Imperial College London Tel: +44 (0)20 7594 9537 r.endres(a)imperial.ac.uk Personal: http://www.imperial.ac.uk/people/r.endres Group: http://www3.imperial.ac.uk/biologicalphysics ________________________________________ From: Alfonso Martinez Arias [ama11(a)hermes.cam.ac.uk] Sent: 05 February 2012 10:13 To: Endres, Robert G Subject: PDRA in image analysis Dear Robert, With apologies for the trouble I wonder if you could post or pass this around. I am looking for a postdoc who is interested in image analysis and modelling and your department should be a good place to find such a person. With best wishes Alfonso Alfonso Martinez Arias Department of Genetics University of Cambridge Cambridge CB2 3EH UK Post-Doctoral Research Associate ------------------------------------------------------------------------ Applications are invited for a postdoctoral researcher to join an interdisciplinary research group and participate in a European Research Council (ERC) funded project on the dynamics of gene expression during stem cell differentiation. You should have experience in biological image analysis and knowledge of software tool development, with a focus on usability, and an interest in working with experimentalists. Knowledge of microscopy will be an advantage. http://www.jobs.cam.ac.uk/job/-12592/

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