- FILM-Users - imperial.ac.uk

[FILM-Users 00187] FWD: postdoc positions
by Martin Spitaler 26 Mar '12

26 Mar '12

...forwarded, for your information, Martin =============================================================== -------- Original Message -------- Date: Mon, 26 Mar 2012 13:36:01 +0100 From: Purbhoo, Marco <marco.purbhoo(a)imperial.ac.uk> To: Spitaler, Martin <m.spitaler(a)imperial.ac.uk> Hi Martin I’d be grateful if you could circulate this post-doc ad on the FILM use list, as it may be of interest. Cheers Marco _______________________________________________________ Marco Purbhoo, D. Phil. Lecturer in Hepatology Imperial College London / St. Mary’s Campus Section of Hepatology & Gastroenterology Division of Diabetes, Endocrinology & Metabolism Department of Medicine Variety Wing, Room VD2 Norfolk Place, London W2 1PG United Kingdom Tel: +44 207 5949024 10010 North Torrey Pines Road, La Jolla, CA 92037-1099 PO Box 85800, San Diego, CA 92186-5800 Email: blillemeier(a)salk.edu www.salk.edu Nomis Center for Immunobiology and Microbial Pathogenesis Waitt Advanced Biophotonics Center Postdoctoral Position at the Salk Institute for Biological Studies, San Diego, USA Lillemeier Laboratory (IMPL-L) Nomis Center for Immunobiology and Microbial Pathogenesis & Waitt Advanced Biophotonics Center The laboratory studies the spatio-temporal organization of the plasma membrane and its role in T cell antigen recognition, activation and signaling. In addition, we are investigating other aspects of membrane biology, e.g. virus-host interactions and membrane aging. The laboratory uses a combination of cutting edge fluorescence microscopy techniques (e.g. super-resolution microscopy and correlation spectroscopy), and traditional biochemical and molecular biological approaches in different model systems. Postdoctoral positions for Biologists, Biophysicists, Bioengineers or Physicists are available: • Multiple projects investigate the structure and function of the plasma membrane during T cell activation, cell aging or infection. These studies utilize state-of-the-art imaging methods. Applicants with a keen interest in life sciences and some background/experience in fluorescence imaging or spectroscopy techniques are preferred. • Additional projects examine qualitative and quantitative differences in signal transduction that determine T cell faith and function. To this end, we use innovative biochemical and molecular biological approaches. For these studies a background in molecular biology and biochemistry is preferred. Please send curriculum vitae, a statement of research interests and the names of three referees to: blillemeier(a)salk.edu Björn Lillemeier, Ph.D. Nomis Center for Immunobiology and Microbial Pathogenesis The Salk Institute 10010 North Torrey Pines Rd. La Jolla, CA 92037 USA

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[FILM-Users 00186] FILM Club postponed
by Martin Spitaler 26 Mar '12

26 Mar '12

Dear microscopists, unfortunately we have to postpone this months FILM Club. The room I had booked was double-booked (not in my control), and neither myself nor Steve will be here on Friday (just Steve left), so it's hard to improvise something. Sorry for that. But you can look forward to an interesting FILM Club next month: Charanjit Singh (Eric Alton lab): 'Quantitative imaging of plasmid DNA in live human airway epithelial cells following non-viral gene transfer, using electron & confocal microscopy'. Fri 27 April 2012, 13.00, room G47A Flowers Building (changed location!). Charanjit's project is linked to the large CFTR gene therapy project which recently attracted much media attention. Make sure you don't miss out on the microscopy behind the headlines. Good imaging, Martin -- ------------------------------------------------------------------------ *Martin Spitaler, PhD* *FILM - Facility for Imaging by Light Microscopy* - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk <mailto:m.spitaler@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

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[FILM-Users 00185] new user charges
by Martin Spitaler 26 Mar '12

26 Mar '12

Dear microscopists, based on the latest usage figures and cost calculations, the new FILM charges have now been calculated, which will be used from *next quarter (1 April 2012)*. In line with Tony's message last Friday, you will notice that due to good usage confocals are now indeed cheaper than before (down 5% from £40 to £38 per hour). So it really does work - the more you use FILM, the cheaper it gets! And thanks to all our users for your loyalty! Also worth noting is that our own staff time is relatively cheap. If you have complicated experiments, it's well worth considering booking 2-3 hours of our dedicated time to get them off the ground, especially at the very beginning of a microscopy project. It might well be cheaper than spending a long time on your own trying to get it to work. As ever, we don't charge for the 2-3 hour training sessions, and we are always happy to give help and advice, but for more demanding projects, dedicated staff time is a good opportunity to get more continuous support time from us. *The new charging rates from 1 April 2012 are:* * Widefield microscopes: £24 per hour * Confocal microscopes: £38 per hour * Multiphoton laser: £24 per hour (in addition to the standard confocal charge) * Software (Volocity and Definiens): £5 per hour * Staff assistance: £31 per hour -> general assistance and training remain free!! For your information: Based on the FEC calculations by Research Support Services and taking into account the latest usage figures and cost caluclations, the charges are decided by the FILM steering committee, consisting of professors Tony Magee (head of FILM), Mike Ferenczi and Dan Davis and myself. For grant applications, please always use the rates published by Research Support Services. Follow the link on the FILM website / GUIDELINES / CHARGES / GRANT APPLICATIONS / RSS WEBSITE and scroll down to CENTRAL FACILITY CHARGE OUT LISTS. <http://www3.imperial.ac.uk/portal/page/portallive/imagingfacility/facility_…>. Good imaging, Martin PS: if you missed Tony's email, see the FILM Mailman archive at <http://mailman.ic.ac.uk/pipermail/film-users/2012/000179.html> -- ------------------------------------------------------------------------ *Martin Spitaler, PhD* *FILM - Facility for Imaging by Light Microscopy* - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk <mailto:m.spitaler@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

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[FILM-Users 00184] Fwd: Alexa Fluor anti-rat
by Martin Spitaler 23 Mar '12

23 Mar '12

...can anybody help? Martin -------- Original Message -------- Subject: Alexa Fluor anti-rat Date: Wed, 21 Mar 2012 16:54:05 +0000 From: Grice, Christopher <c.grice11(a)imperial.ac.uk> To: Spitaler, Martin <m.spitaler(a)imperial.ac.uk> Dear Martin, Could you please forward this to the FILM members please? Dear all, I was wondering if anyone has a sample of an Alexa Fluor anti-rat antibody I could acquire? Many thanks in advance for any help you can provide, Chris Grice 5^th Floor Flowers Building.

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[FILM-Users 00183] Fwd: Photonex Roadshow London 2012, April 26th
by Martin Spitaler 23 Mar '12

23 Mar '12

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[FILM-Users 00180] FILM - the more you use it, the cheaper it gets!
by Martin Spitaler 23 Mar '12

23 Mar '12

Subject: FILM - the more you use it, the cheaper it gets! Date: Thu, 22 Mar 2012 16:59:58 +0000 From: Magee, Tony <t.magee(a)imperial.ac.uk> Dear FILM User, *FILM - the more you use it, the cheaper it gets!* The FILM management team has just met to set the*charges for next year* – this has *important implications for you* as users that I want to bring to your attention. You may not be aware of the financial model FILM operates on – College requires us to recover /all/ our costs but /not/ to make a surplus. You can imagine this is not a straightforward challenge, but we do our best and so far have thankfully broken even or made only a slight surplus! To set our charges for the upcoming year, in essence what we do is divide our costs (which are more or less fixed – service contracts, consumables/minor equipment and salaries) by the number of hours usage of the microscopes and other services we offer. So you can see that this simple calculation means that the more the microscopes get used, the less we can charge for usage in the next year – so, *the more you use FILM, the cheaper it gets!* ** The “catch” is that the savings are made in the /following/ year, i.e. there is a lag. Nevertheless, if you are a regular and long term user, you can use FILM more and it will get cheaper, so you get more use for the same amount of money in the medium/long term! There /is /such a thing as a free lunch, and FILM provides it! *Please use FILM as much as you can – it benefits us all!!* Many thanks. Tony Anthony I. Magee Professor of Membrane Biology Section of Molecular Medicine National Heart and Lung Institute Faculty of Medicine Imperial College London Sir Alexander Fleming Building Exhibition Road South Kensington London SW7 2AZ UK Tel. +44 (0)20 7594 3135 FAX +44 (0)20 7594 3015 http://www1.imperial.ac.uk/nhli/molecular/lipidation/ <blocked::http://www1.imperial.ac.uk/nhli/molecular/lipidation/> http://www1.imperial.ac.uk/medicine/people/t.magee/ *****Regulation of protein trafficking and function by palmitoylation**** *St Anne's College, Oxford, UK. 23 August - 25 August 2012 Visit: http://www.biochemistry.org/Focused.aspx

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[FILM-Users 00181] Fwd: Bagrit Lecture by Professor Ed Boyden, MIT - “Optogenetics and other tools for the analysis of neural circuits” - 14 May 2012
by Martin Spitaler 23 Mar '12

23 Mar '12

...forwarded, for your information, Martin ========================= -------- Original Message -------- Subject: [High-Throughput-Technology-Community] 2012 Bagrit Lecture by Professor Ed Boyden, MIT - “Optogenetics and other tools for the analysis of neural circuits” - 14 May 2012 Date: Fri, 23 Mar 2012 13:51:17 +0000 From: Fenton, Rob J <r.fenton(a)imperial.ac.uk> 2012 Bagrit Lecture Optogenetics and other tools for the analysis of neural circuits Professor Ed Boyden, MIT Date: Monday 14 May 2012 Time: 17.30 Venue: Lecture Theatre G16, Sir Alexander Fleming Building, South Kensington Campus (building no 33 on the map). RSVP: Angela Glyes a.glyes(a)imperial.ac.uk Understanding how neural circuits implement brain functions, and how these computations go awry in brain disorders, is a top priority for neuroscience. Over the last several years we have developed a rapidly-expanding suite of genetically-encoded reagents that, when expressed in specific neuron types in the nervous system, enable their electrical activities to be powerfully and precisely activated and silenced in response to pulses of light. These tools are in widespread use for analyzing the causal role of defined cell types in normal and pathological brain functions. In this talk I will briefly give an overview of the field, and then I will discuss a number of new tools for neural activation and silencing that we are developing, including new molecules with augmented amplitudes, improved safety profiles, novel color and light-sensitivity capabilities, and unique new capabilities. We have begun to develop hardware to enable complex and distributed neural circuits to be precisely controlled, and for the network-wide impact of a neural control event to be measured using distributed electrodes, fMRI, and robotic intracellular neural recording. We explore how these tools can be used to enable systematic analysis of neural circuit functions in the fields of emotion, sensation, and movement, and in neurological and psychiatric disorders. Finally, we discuss our pre-clinical work on translation of such tools to support novel ultraprecise neuromodulation therapies for human patients. Professor Ed Boyden Ed Boyden is the Benesse Career Development Professor, and Associate Professor of Biological Engineering and Brain and Cognitive Sciences, at the MIT Media Lab and the MIT McGovern Institute. He leads the Synthetic Neurobiology Group, which develops tools for controlling and observing the dynamic circuits of the brain, and uses these neurotechnologies to understand how cognition and emotion arise from brain network operation, as well as to enable systematic repair of intractable brain disorders such as epilepsy, Parkinson's disease, post- traumatic stress disorder, and chronic pain. The tools his group has invented include a suite of 'optogenetic' tools that are now in use by hundreds of groups around the world, for activating and silencing neurons with light. These tools enable the causal assessment of how specific neurons contribute to normal and pathological brain functions, revealing with great temporal precision the processes for which their activities are necessary or sufficient. He has launched an award-winning series of classes at MIT that teach principles of neuroengineering, starting with basic principles of how to control and observe neural functions, and culminating with strategies for launching companies in the nascent neurotechnology space. He was named to the "Top 35 Innovators Under the Age of 35" by Technology Review in 2006, and to the "Top 20 Brains Under Age 40" by Discover Magazine in 2008. He has received the NIH Director's New Innovator Award, the Society for Neuroscience Research Award for Innovation in Neuroscience, the NSF CAREER Award, and the Paul Allen Distinguished Investigator Award. His work was in 2010 recognized as the "Method of the Year" by the journal Nature Methods, and in 2011, he delivered a lecture on optogenetics at TED. Ed received his Ph.D. in neurosciences from Stanford University as a Hertz Fellow, where he discovered that the molecular mechanisms used to store a memory are determined by the content to be learned. Before that, he received three degrees in electrical engineering and physics from MIT. He has contributed to over 250 peer-reviewed papers, current or pending patents, and articles, and has given over 140 invited talks on his work.

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[FILM-Users 00179] Fwd: FW: Senior Scientific Officer in High Throughput Microscopy
by Martin Spitaler 22 Mar '12

22 Mar '12

...forwarded, for your information, Martin -------- Original Message -------- Subject: FW: Senior Scientific Officer in High Throughput Microscopy Date: Wed, 21 Mar 2012 19:45:27 +0100 From: Stefan Terjung <terjung(a)embl.de> Reply-To: <terjung(a)embl.de> Organization: EMBL To: <terjung(a)embl.de> FYI *From:*Rainer Pepperkok [mailto:pepperko@embl.de] Dear colleagues, we presently have an opening for a senior scientific officer in high throughput microscopy (see link below). I would be most grateful if you could forward this mail to anyone you think it could be of interest. Thank you very much! Rainer Pepperkok You can find out more about this vacancy at: http://www.embl.de/aboutus/jobs/searchjobs/index.php?newms=jj&id=48380&newl… <http://www.embl.de/aboutus/jobs/searchjobs/index.php?newms=jj&id=48380&newl…>

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[FILM-Users 00178] Fwd: TODAY! Invitation to the inaugural lecture of Professor Ralph Knöll
by Martin Spitaler 21 Mar '12

21 Mar '12

...forwarded, for your information, Martin -------- Original Message -------- Subject: TODAY! Invitation to the inaugural lecture of Professor Ralph Knöll Date: Wed, 21 Mar 2012 09:16:24 +0000 From: Watson, Emma M <e.watson(a)imperial.ac.uk> To: Faculty of Medicine, NHLI <icsm-nhli-dl(a)imperial.ac.uk> The honour of your company is requested at an inaugural lecture: "Molecular mechanisms and future therapies in heart failure" Professor Ralph Knöll, Chair in Myocardial Genetics Date: Wednesday 21 March 2012 Time: 17.30 – 18.30 Venue: Lecture Theatre G16, Sir Alexander Fleming Building, South Kensington Campus (building no 33 on the map). Travel information for the South Kensington Campus can be found on the Imperial website. A pre-lecture tea will take place from 16.45 in the Foyer, Sir Alexander Fleming Building. RSVP: Emma Watson e.watson(a)imperial.ac.uk Cardiovascular diseases such as heart failure are the most common cause of death globally. Although major advances have been achieved during the last decades, our understanding of the underlying molecular mechanisms, necessary to develop new therapeutic strategies, remains incomplete. One such mechanism is mechanosensation, the fundamental process by which cells sense mechanical stimuli and convert them into biomechanical signals. Mechanosensation is also linked to mechanoptosis, a recently described type of cell death only found after an increase in biomechanical stress in striated muscles such as the heart. A diverse group of diseases has now been linked to mutations in the Z-disc proteins that form the segments separating the sarcomeres – the smallest functional units of striated muscle. These Z-discopathies can lead to perturbations in mechanosensation and apoptosis. Developing ways of combating cardiac myocyte cell death is key to finding ways to combat these lethal conditions. Professor Knöll was recruited to Imperial as a Professor and Chair in Myocardial Genetics in October 2009 from the Georg August University, Göttingen, Germany, where he was a Professor as well as Head of the Working Group in Cardiovascular Molecular Genetics since 2004. He was a Postgraduate Researcher and then Group Leader at the Institute of Molecular Medicine, UC San Diego, from 1999 to 2004. He did his clinical training at the Charité in Berlin and his professional training at the Max-Planck-Institute of Physiological and Clinical Research, Department of Experimental Cardiology, and was educated at Johann Wolfgang Goethe University Medical School, Frankfurt and Justus Liebig University Medical School, Giessen. Professor Knöll is a referee for a number of prominent journals, including Circulation, Molecular and Cellular Biochemistry, European Journal of Heart Failure, and Clinical Cardiology. He is a Member of the European Society of Cardiology, the American Heart Association, and the German Cardiac Society, and has won numerous awards including the Theodor Frerichs Award from the German Society for Internal Medicine and the Fritz Acker Award from the German Cardiac Society.

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[FILM-Users 00177] FW: Register for the Correlative Microscopy Webinar
by Martin Spitaler 21 Mar '12

21 Mar '12

...forwarded, for your information, Martin *From:*Cell Press [mailto:cellpress@mail.cell-press.com] *Sent:* 20 March 2012 19:40 *Subject:* Register for the Correlative Microscopy Webinar Cell Press Register for a Free Webinar ------------------------------------- Correlative Microscopy ------------------------------------- Live date: Wednesday, April 4, 2012 12:00 Noon US ET -------------------------------------Register Today, It's Free... Hear about the latest applications of correlative microscopy from Dr. John Briggs, Dr. Mark Ellisman, Dr. Carolyn Larabell, and Dr. Peijun Zhang during this exciting webinar from Cell Press. Light microscopy has been providing information on the location of key molecules that can be tracked by staining or fusion to fluorescent proteins at ever increasing resolution, while electron microscopy has traditionally been used to provide high resolution views of the overall ultrastructure of samples. New approaches and equipment are now allowing more scientists to combine these two techniques to provide a unified view of the location of biological molecules in the context of the full structure of a sample, and these are collectively referred to as correlative microscopy. Register Today, It's Free...: <http://mediazone.brighttalk.com/event/ReedElsevier/6de59d960d-5973-intro> Unable to join us on April 4? This webinar will be archived for 6 months for on-demand viewing. Register today to ensure you receive updates on how to gain access after the live event! Be sure to check out our archive of past webinars at http://www.cell.com/webinars Moderated by: Paul Wiseman, Associate Professor of Physics and Chemistry, McGill University Speakers: Carolyn LarabellCarolyn Larabell National Center for X-ray Microscopy John BriggsJohn Briggs Cell Biology and Biophysics Unit, European Molecular Biology Laboratory Peijun ZhangPeijun Zhang Department of Structural Biology, University of Pittsburgh Mark EllismanMark Ellisman National Center for Microscopy and Imaging Research Supporting Journal: Biophysical Journal Cell Press

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