- FILM-Users - imperial.ac.uk

[FILM-Users 00239] FW: FILM registration confirmation
by Rothery, Stephen M 18 Jul '12

18 Jul '12

Hi All Does anyone have any experience using luciferase (luc2 in particular) to measure cell viability? would welcome any advice Thanks so much, Allie Alessondra Speidel a.speidel11(a)imperial.ac.uk<mailto:a.speidel11@imperial.ac.uk>

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[FILM-Users 00238] FW: FLIPR ?
by Rothery, Stephen M 18 Jul '12

18 Jul '12

Can anyone help Eric bw Steve Hi, Would you know where I could have access to a FLIPR system in SAF bld ? (to assess calcium signals) Cheers Eric Dr Eric Dubuis Tel: + 44 (0) 207 594 8427 Fax: + 44 (0) 207 594 3100 Research Fellow in Airway Disease National Heart and Lung Institute Faculty of Medicine Sir Alexander Fleming Building South Kensington Campus

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[FILM-Users 00237] Special Seminar
by Rothery, Stephen M 18 Jul '12

18 Jul '12

TODAY Special Seminar Dr Omar Qureshi University of Birmingham "Understanding trans-endocytosis in immune regulation; how to rip stimulatory molecules from antigen-presenting cells" 12.30 pm Wednesday July 18th Link Lecture Theatre (290) Sir Ernst Chain building (formerly Biochemistry) South Kensington Campus All welcome Host: Murray Selkirk

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[FILM-Users 00236] FILM staff away
by Martin Spitaler 10 Jul '12

10 Jul '12

Dear microscopists, in the coming weeks, we will take turns to go away on meetings and summer holidays, starting with myself from tomorrow afternoon. This means that until mid August, the remaining members of the FILM crew might be a bit stretched when it comes to training and support. We will certainly do our best to respond to your needs as quickly as possible, but please be patient when things take a bit longer than usual to be sorted. I know many of you will be away as well, so it should hopefully work out well. Afterwards, we will be back in business refreshed by hopefully lots of sunshine (hopefully also Steve in Scotland). Good wishes for your own trips or holidays, and good imaging while here, Martin -- ------------------------------------------------------------------------ *Martin Spitaler, PhD* *FILM - Facility for Imaging by Light Microscopy* - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk <mailto:m.spitaler@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

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[FILM-Users 00235] Fwd: RE: Volocity
by Martin Spitaler 10 Jul '12

10 Jul '12

...forwarded for your information; I won't have time, but maybe someone else is interested in being invited by Perkin Elmer to give a presentation about using Volocity for quantification at the European Microscopy Congress in Manchester in September, Martin -------- Original Message -------- Subject: RE: Volocity Date: Tue, 10 Jul 2012 13:22:12 +0000 From: Barlow, Andrew <Andrew.Barlow(a)PERKINELMER.COM> To: Martin Spitaler <m.spitaler(a)imperial.ac.uk> On 09/07/2012 12:22, Barlow, Andrew wrote: Dear Martin. My name is Andrew Barlow, I'm an application specialist working on Volocity. I understand that you co-ordinate a number of Volocity systems. I'm currently searching for a user of Volocity quantitation that might be prepared to give a presentation at a PerkinElmer workshop at the European Microscopy Congress in Manchester. Would you be able to suggest anybody that might be interested? PE would be able to offer travel and accommodation expenses for speakers. I'm also interested to hear how your users are progressing with the new Quantitation features added in Volocity 6. Do you feel that they would benefit from a visit from us for some training and advice? Regards, Andrew ** Andrew Barlow PhD | Applications Specialist PerkinElmer | For the Better Andrew.Barlow(a)perkinelmer.com <mailto:Andrew.Barlow@perkinelmer.com> Phone: +44 2476 692229| Fax: +44 2476 690091 | Mobile: +44 7799 795 999 Millburn Hill Road, Coventry, CV4 7HS, UK www.perkinelmer.com <http://www.perkinelmer.com> *Please consider the environment before printing this e-mail.* This e-mail message and any attachments are confidential and proprietary to PerkinElmer, Inc. If you are not the intended recipient of this message, please inform the sender by replying to this email or sending a message to the sender and destroy the message and any attachments. Thank you.

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[FILM-Users 00234] New Huygens deconvolution software
by Martin Spitaler 05 Jul '12

05 Jul '12

Dear microscopists, as part of the equipment purchase for the L-Block (NHLI) in Hammersmith, we have also got a licence for the powerful deconvolution software SVI Huygens, including modules for CONFOCAL DECONVOLUTION and MULTIPHOTON DECONVOLUTION. Advantages over our existing Autoquant deconvolution: # additional modules for confocal and multiphoton deconvolution # more control over the deconvolution settings # centralised installation, accessible from everywhere in the college # better batch processing (including line command option) The software is installed on a server of the Bioinformatics Support Service, so it can be used from anywhere in the college. At the moment we have 2 concurrent user licences, so 2 people can use the software in parallel at any time. Prioritisation is via the FILM HUYGENS booking page on Sharepoint. To get access, please email to set up your access and give you all required information. Charging is at our normal software charging rate, see FILM website / GUIDELINES / CHARGES for the latest rates (according to automated usage logs). If you want to use the software, please get in touch. Good imaging, Martin PS: thanks all of you who came along and contributed to the success of the Microscopy Day, it's been a very productive event, with lots of discussions, and the presentations of the Matchmaker session and posters were very interesting -- ------------------------------------------------------------------------ *Martin Spitaler, PhD* *FILM - Facility for Imaging by Light Microscopy* - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk <mailto:m.spitaler@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

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[FILM-Users 00233] TODAY: FILM Microscopy Day 2012
by Martin Spitaler 29 Jun '12

29 Jun '12

Dear microscopists, just a reminder TODAY is the FILM MICROSCOPY DAY - EVERYBODY IS WELCOME (you don't need to be a FILM user) There will also be a short overview of FILM for new people, and WHAT'S NEW for existing users, e.g. the new microscopes in Hammersmith, new Huygens deconvolution software (including confocal and multiphoton modules), just before the match maker session >>AGENDA<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< 10.00- 10.10 Welcome by Prof Tony Magee, head of FILM GENERAL MICROSCOPY TECHNIQUES 10.10-10.55 Basics of microscopy - magnification, properties of light, resolution, transmitted light contrast (PH, DIC, darkfield), epifluorescence, confocal microscopy (Martin Spitaler, FILM) 10.55-11.40 Live imaging techniques - Multiphoton, live imaging and second harmonic generation (SHG) (Mark Scott, FILM) 11.40-12.00 coffee & tea break 12.000-13.00 MICROSCOPY MATCHMAKER: FILM users giving a snapshot of their work in 2-minute presentations Introduction: FILM overview, what's new in FILM (Martin Spitaler) # David Hodson: tba # Katrin Kronenberger: Optimisation of whole mount imaging # Malak Al-Yamani & Martina Lundberg: Imaging the inside wall of the mouse aortic arch # Mirella Koleva: Study of Sonic hedgehog multimerisation # Nickolai Vysokov: Role of Latrophilin-Lasso trans-synaptic intreaction # Paulina Ciepla: tba # Sophie Pageon: Imaging the nanoscale organisation of KIR2DL1 using PALM 13.00-14.15 Poster session with sandwich lunch, ground floor (outside cafeteria) (kindly sponsored by CARL ZEISS MICROSCOPY) SPECIAL TECHNIQUES 14.15-15.00 Fluorophores as sensors - colocalisation, fluorescence lifetime (FLIM) and resonant energy transfer (FRET) (Valentina Caorsi, NHLI) 15.00-15.45 Super-resolution microscopy - TIRF, PALM, STORM (Steve Rothery, FILM) 15.45-16.05 coffee & tea break UNDERSTANDING IMAGES 16.05-16.30 Image data: data formats, pixel and metadata, image visualisation and presentation, OMERO database (Martin Spitaler, FILM) 16.30-17.35 Image analysis: noise removal, deconvolution, segmentation, tracking, registration (Luis Pizarro, Computing) 17.35-17.40 Wrap-up, feedback, outlook For more and up-to-date information, please check <http://www3.imperial.ac.uk/portal/page/portallive/imagingfacility/news>. See you all there, good imaging, Martin ############################################# -- Martin Spitaler, PhD FILM - Facility for Imaging by Light Microscopy - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk Website: http://imperial.ac.uk/imagingfacility

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[FILM-Users 00232] COMMERCIAL Fwd: Badrilla antibodies
by Martin Spitaler 27 Jun '12

27 Jun '12

...forwarded for your information; By the way, FILM users: I'm careful not to flood your inboxes with commercial emails, but thought it could be interesting, and ok if it's clearly stated in the subject; please let me know what you think about forwarding special offers and other commercial information, Martin -------- Original Message -------- Subject: A request for some help Date: Wed, 13 Jun 2012 04:44:05 -0700 From: Gavin Allsop <g.allsop(a)badrilla.com> To: <m.spitaler(a)IMPERIAL.AC.UK> Dear Martin. I hope that you are well. I wonder if you could help? I completed my PhD in 2010 at the University of Leeds in the School of Molecular and Cellular Biology, this project was largely based around microscopy and molecular biology. I now work for Badrilla Ltd <http://badrilla.com/>, a UOL spin-off company whose goal is to improve the supply of good quality antibodies for university research. In my experience as a researcher, this is long overdue. We produce well validated antibodies, presented honestly in real-world experimental settings. I hope to make a positive contribution to the industry. Here’s the request for help. Please would you circulate the technical details of my first wave of products (c-Myc <http://badrilla.com/gfp-polyclonal-5.html> | GFP <http://badrilla.com/gfp-polyclonal-5.html> | mCherry <http://badrilla.com/pmcherry-5.html>) to your research colleagues and inform them that further antibodies to beta-tubulin <http://dl.dropbox.com/u/41917315/Data%20Sheets/A010-bTub.pdf>, GST <http://dl.dropbox.com/u/41917315/Data%20Sheets/A010-GST.pdf> and MBP <http://dl.dropbox.com/u/41917315/Data%20Sheets/A010-MBP.pdf> will be released in the coming months. We hope to become a trusted and more affordable alternative to the industries giants for UK universities. With best regards Gavin -------------- Dr Gavin Allsop Badrilla Ltd Leeds Innovation Centre 103 Clarendon Road Leeds LS2 9DF United Kingdom -------------- http://badrilla.com Twitter <http://twitter.com/@BadrillaLtd>Facebook <http://facebook.com/BadrillaLtd>

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[FILM-Users 00231] reminder - THIS FRIDAY: FILM Microscopy Day 2012
by Martin Spitaler 25 Jun '12

25 Jun '12

Just a reminder about THIS FRIDAY'S FILM MICROSCOPY DAY. And be aware, EVERYBODY IS WELCOME (you don't need to be a FILM user, feel free to forward this to anybody else), but if to help with catering, please register with Ruth A O'Donnell <r.odonnell(a)imperial.ac.uk> if you haven't done so yet. And if interested, one or two slots for the Matchmaker presentation or poster session are also still available - be quick. Martin -------- Original Message -------- Subject: FILM Microscopy Day 2012 Date: Mon, 11 Jun 2012 14:25:36 +0100 From: Martin Spitaler <m.spitaler(a)imperial.ac.uk> To: FILM-Users mailing list <film-users(a)imperial.ac.uk> CC: Ruth A O'Donnell <r.odonnell(a)imperial.ac.uk> Dear microscopists and supervisors, the FILM MICROSCOPY DAY 2012 is now only just over two 2 weeks - so if you haven't done so yet, please register NOW! With all the extra bank holidays you might have forgotten about it, but you need to register your slot on the Matchmaking Session, your chance to get new ideas, start networking, find solutions to your microscopy problems. Or if you really can't make that, at least your slot in the poster session, or at the very least to make sure there is enough catering! SUPERVISORS and PIs: I seems that students and postdocs need a bit of a push to take part in our Matchmaking Session. We do believe that this is an excellent opportunity for them to present the work they are doing (advertise your lab), find networking opportunities and on the side practice their presentation skills. So please make sure they don't miss out and convince them to register and take it up. For details, see below. THE EXTENDED REGISTRATION DEADLINE IS FRIDAY 15 JUNE 2012 Good imaging, Martin ======================================================== Dear microscopists, on FRIDAY 29 JUNE we will have the FILM MICROSCOPY DAY 2012 - as in previous years, a day full of talks on all aspects of light microscopy, from the very basics to very advanced technologies (in vivo imaging, super-resolution, non-linear techniques). The presentations will be by FILM staff (Steve, Mark and myself) supported by two experts in their field, VALENTINA CAORSI (NHLI) on non-linear fluorescence techniques (fluorescence lifetime imaging and its various applications) and LUIS PIZARRO (COMPUTING) on image analysis. We also have a special novelty: To make people aware what's going on around them and stimulate new collaborations, we will have a MICROSCOPY MATCH MAKER, where you FILM users are all invited to present your exciting research projects in a short snapshot oral presentation and / or a poster. Oral presentations are strictly limited to a MAXIMUM OF 2 MINUTES / 2 POWERPOINT SLIDES - longer discussions will be possible at the posters over lunchtime. An excellent opportunity to initiate new collaborations, don't miss out - PIs, please encourage your students and postdocs to participate. Please REGISTER BY FRIDAY 15 JUNE 2012 by email to Ruth O'Donnell <r.odonnell(a)imperial.ac.uk>, including your MATCHMAKER OPTIONS (presentation, poster, none). >>PROGRAMME<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< 10.00- 10.10 Welcome by Prof Tony Magee, head of FILM GENERAL MICROSCOPY TECHNIQUES 10.10-10.55 Basics of microscopy - magnification, properties of light, resolution, transmitted light contrast (PH, DIC, darkfield), epifluorescence, confocal microscopy (Martin Spitaler, FILM) 10.55-11.40 Live imaging techniques - Multiphoton, live imaging and second harmonic generation (SHG) (Mark Scott, FILM) 11.40-12.00 coffee & tea break 12.000-13.00 MICROSCOPY MATCHMAKER: FILM users giving a snapshot of their work in 2-minute presentations 13.00-14.15 Poster session with sandwich lunch (kindly sponsored by CARL ZEISS MICROSCOPY) SPECIAL TECHNIQUES 14.15-15.00 Fluorophores as sensors - colocalisation, fluorescence lifetime (FLIM) and resonant energy transfer (FRET) (Valentina Caorsi, NHLI) 15.00-15.45 Super-resolution microscopy - TIRF, PALM, STORM (Steve Rothery, FILM) 15.45-16.05 coffee & tea break UNDERSTANDING IMAGES 16.05-16.50 Understanding and visualisation of image data: data formats, pixel and metadata, image visualisation and presentation, OMERO database (Martin Spitaler, FILM) 16.50-17.35 Image analysis: noise removal, deconvolution, segmentation, tracking, registration (Luis Pizarro, Computing) 17.35-17.40 Wrap-up, feedback, outlook For more and up-to-date information, please check <http://www3.imperial.ac.uk/portal/page/portallive/imagingfacility/news>. A flyer is also available <https://workspace.imperial.ac.uk/imagingfacility/Public/FILM%20Microcopy%20…> See you all there. Good imaging, Martin -- ------------------------------------------------------------------------ *Martin Spitaler, PhD* *FILM - Facility for Imaging by Light Microscopy* - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk <mailto:m.spitaler@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

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[FILM-Users 00230] Fwd: Beautiful Science Exhibition is here!!!
by Martin Spitaler 21 Jun '12

21 Jun '12

...forwarded, for your information: A very interesting event coming up, grown out of a postdoc project involving several FILM users to combine science and art, Martin -------- Original Message -------- Subject: Beautiful Science Exhibition is here!!! Date: Thu, 21 Jun 2012 12:03:21 +0100 From: Archanco, Marta <m.archanco(a)imperial.ac.uk> Dear All, Can you please help us distributing the below email to your contacts? We would like to see us advertised everywhere but specially interested in getting it in more art/design media and from any country (we already have a journal from Australia that is writing about us!!!). Any help with this will be very much appreciated. Many thanks for your help and we hope you will all enjoy the exhibition. Kind regards, Marta Archanco Beautiful Science Project Manager /We would like to invite you to join us at an exhibition exploring the relationship between Science and Art./ /cid:1DFA968E-08E1-4554-8793-231630067D08/ *26th June - 2nd July, 1- 6 pm* *The Brick Lane Gallery (Annexe)* 196 Brick Lane, E1 6SA London *Is there beauty in raw scientific data?* A group of Imperial College scientists think so -- and have collaborated with artists in an experiment to translate and communicate this beauty to an audience of art-goers. The relationship between the raw data and artistic interpretation varies across a spectrum from literal and direct engagement to highly abstract, evocative works that bear only the echo of scientific inspiration. The scientific data on display encompasses photography of world invisible to the naked eye and the graphs, charts and records that are part and parcel of scientific practice. The artworks range across media, encompassing film, photography, painting and design. More details: beautiful.science(a)imperial.ac.uk <mailto:beautiful.science@imperial.ac.uk> http://www.beautifulscience.info/ http://www.facebook.com/beausci <http://www.beautifulscience.info/> <http://www.beautifulscience.info/> <http://www.beautifulscience.info/> *Supported by:* Imperial College, WellCome Trust, Kingston University, ELGA, KIMTECH

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