- FILM-Users - imperial.ac.uk

[FILM-Users 00820] FLOW/FILM user meeting 17/10 14:00 - 16:00
by Bruckbauer, Andreas 24 Sep '18

24 Sep '18

Dear all, The FILM and Flow facilities will be having a combined user meeting on Wednesday the 17th of October from 14:00 - 16:00 (free cookies!!!). This will be on the 6th floor SAF in the glass walled meeting area. FLOW will be hosting the first 60 minutes and FILM the second, you can choose whether you come to one or both and both sessions will be in the following format: * 15 minute presentation by the facility. * A chance for users to discuss problems or helpful hints from their own data. * Feedback for the facility. If you would like a chance to present your work in the presentation slot, please contact me! Best wishes Andreas ---------------------------------------------------- Dr Andreas Bruckbauer FILM facility manager Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 401 +44 (0)20 7594 2023 a.bruckbauer(a)imperial.ac.uk<mailto:a.bruckbauer@imperial.ac.uk> http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ ----------------------------------------------------

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[FILM-Users 00819] Herston Biofabrication Institute (QUT, Aus) PhD and Postdoc Positions Available!
by Gaboriau, David C A 20 Sep '18

20 Sep '18

Dear Users, Please see attached for PhD and Post-Doc positions in Brisbane, Australia. All the best, David __________ The Herston Biofabrication Institute under Prof. Mia Woodruff is accepting PhD Candidate and Postdoctoral Research Associate applications for all of its initial 2019 clinical streams: orthopedic, vascular, skin, and urology amongst the cores of biofabrication: 3D imaging, 3D modeling, and 3D printing toward medical devices. Accepted candidates are earmarked for generous bursaries, expected to start in early 2019 (flexible), and will be located in sunny Brisbane, Australia. The Herston Biofabrication Institute is supported by the Queensland University of Technology and Queensland Metro North Hospitals and Health Services and is located in the Royal Brisbane and Women’s Hospital, Herston precinct. Deadlines are quickly approaching - do apply soon. See the attached advert for details. David Gaboriau, PhD FILM – Facility for Imaging by Light Microscopy High-Content Microscopy Specialist Sir Alexander Fleming Building, desk 408 Imperial College London / South Kensington Exhibition Road London SW7 2AZ, UK Phone: +44 (0)2075 949 793 Email: d.gaboriau(a)imperial.ac.uk<mailto:d.gaboriau@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility Page: http://www.imperial.ac.uk/people/d.gaboriau

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[FILM-Users 00818] SAFB heating shutdown tomorrow!
by Bruckbauer, Andreas 17 Sep '18

17 Sep '18

Dear FILM users, There will be a heating shutdown in the SAF building tomorrow, this might also affect air-conditioning in the microscope rooms, please see below. If the temperature exceeds 25 degrees (due to no air conditioning) we might need to stop the microscopes. Best wishes Andreas From: Wigley, Fraser Sent: 17 September 2018 08:37 To: Building, Sir Alexander Fleming <est-sir-alexander-fleming-building-dl(a)imperial.ac.uk<mailto:est-sir-alexander-fleming-building-dl@imperial.ac.uk>> Subject: SAFB heating shutdown tomorrow Dear All, An isolation valve in the SAFB hot water system has failed and needs to be replaced. There will be a shutdown starting tomorrow morning. Start: 10:00 on Tuesday 18 September End: 10:00 on Wednesday 19 September Services affected: All heating in SAFB, including AHUs (temperature and humidity) and FCUs, 37C CT rooms, hot water from taps Services not affected: Cooling in AHUs and FCUs, electric heating The timing of this shutdown has been chosen to minimise impact on teaching, research and meetings. I apologise for any inconvenience caused. Regards, Fraser Fraser Wigley Building Manager Sir Alexander Fleming | Chemistry | Dyson | Sir Ernst Chain | Flowers | Observatory | RCS1 www.imperial.ac.uk/people/f.wigley<http://www.imperial.ac.uk/people/f.wigley> [IIP_ACRED_LOGO_2021] ---------------------------------------------------- Dr Andreas Bruckbauer FILM facility manager Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 401 +44 (0)20 7594 2023 a.bruckbauer(a)imperial.ac.uk<mailto:a.bruckbauer@imperial.ac.uk> http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ ----------------------------------------------------

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[FILM-Users 00817] Workshop on BioImage Analysis with Icy for FILM Users
by Gaboriau, David C A 03 Sep '18

03 Sep '18

Dear all, I am organising a one-day free workshop on BioImage Analysis for users of the facility, using the open-source software Icy. The workshop is an introduction to Icy and will cover how to manipulate and annotate images, how to use plugins to analyse data and how to build your own custom analysis sequences using Protocols, a graphical programming tool. No programming/scripting experience necessary. Event: Friday 28th September, 10am-5pm Computer room G28 Sir Alexander Fleming building South Kensington campus https://www.eventbrite.co.uk/e/bioimage-analysis-workshop-with-icy-tickets-… Password to book tickets: FILM Hope to see you there! All the best, David David Gaboriau, PhD FILM - Facility for Imaging by Light Microscopy High-Content Microscopy Specialist Sir Alexander Fleming Building, desk 408 Imperial College London / South Kensington Exhibition Road London SW7 2AZ, UK Phone: +44 (0)2075 949 793 Email: d.gaboriau(a)imperial.ac.uk<mailto:d.gaboriau@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility Page: http://www.imperial.ac.uk/people/d.gaboriau

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[FILM-Users 00816] !!! Old scratch server will be moved on August 31st
by Bruckbauer, Andreas 23 Aug '18

23 Aug '18

Dear FILM-users Our old scratch server will be moved to the data centre in Slough on August 31st, it will be unavailable on that day. Please use the new server: \\film-nas2.med.ic.ac.uk\scratch2 Instruction how to connect are on our webpage under Guidelines\data and network Best wishes Andreas ---------------------------------------------------- Dr Andreas Bruckbauer FILM facility manager Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 401 +44 (0)20 7594 2023 a.bruckbauer(a)imperial.ac.uk<mailto:a.bruckbauer@imperial.ac.uk> http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ ----------------------------------------------------

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[FILM-Users 00815] Samples left on CF6 and oil on 10X
by Gaboriau, David C A 21 Aug '18

21 Aug '18

Dear Users and CF6 Users in particular, I found two slides left on CF6, one next to the keyboard, and one with liquid left on the stage of the microscope itself, see attached pictures. Please make sure you take all your samples with you when you finish your session. If you need to discard your slides, there is a sharps bin in the room. I also saw some damage to the 10X objective, probably caused when inserting the sample holder in the stage. Please be careful not to hit the objective when putting on the stage insert. Finally, the 10X objective was covered in immersion oil. Please only use oil on immersion objectives, and if you add some onto an air objective by mistake, let us know straight away so we can clean it. If you are not sure, please ask us! Thank you for your co-operation, All the best, David David Gaboriau, PhD FILM - Facility for Imaging by Light Microscopy High-Content Microscopy Specialist Sir Alexander Fleming Building, desk 408 Imperial College London / South Kensington Exhibition Road London SW7 2AZ, UK Phone: +44 (0)2075 949 793 Email: d.gaboriau(a)imperial.ac.uk<mailto:d.gaboriau@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility Page: http://www.imperial.ac.uk/people/d.gaboriau

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[FILM-Users 00814] !!! move your data - old scratch server will be disconnected !!!
by Bruckbauer, Andreas 21 Aug '18

21 Aug '18

Dear film-users, The old scratch server will be disconnected very soon, you need to move your data to a safe place to avoid data loss!!! Best wishes Andreas ---------------------------------------------------- Dr Andreas Bruckbauer FILM facility manager Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 401 +44 (0)20 7594 2023 a.bruckbauer(a)imperial.ac.uk<mailto:a.bruckbauer@imperial.ac.uk> http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ ----------------------------------------------------

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[FILM-Users 00813] Scientific Community Image Forum
by Gaboriau, David C A 03 Aug '18

03 Aug '18

Dear Users, The new Scientific Community Image Forum is online at https://forum.image.sc "This forum's focus is software-oriented aspects of scientific imaging, particularly (but not limited to) image analysis, processing, acquisition, storage, and management of digital scientific images. The primary objective is to foster independent learning 4<https://forum.image.sc/guidelines#independent-learning> for everyone in the community." "Several open source software projects have pledged to use this forum as their recommended discussion channel; see the front page 10<https://forum.image.sc/>. Discussion of any software packages in the imaging ecosystem is equally welcome-the goal here is inclusivity. Our mission 2<https://forum.image.sc/faq#mission> is for people to be able to ask "how do I do X?" without prior knowledge of these various software programs. See the Guidelines 2<https://forum.image.sc/guidelines> for further information." Open-source platforms that came together for this forum include FIJI, Icy, CellProfiler, ImageJ, Cytomine, Ilastik, OpenSPIM, BoneJ and others. This is a great resource to ask questions and learn about bioimage analysis. All the best, David David Gaboriau, PhD FILM - Facility for Imaging by Light Microscopy High-Content Microscopy Specialist Sir Alexander Fleming Building, desk 408 Imperial College London / South Kensington Exhibition Road London SW7 2AZ, UK Phone: +44 (0)2075 949 793 Email: d.gaboriau(a)imperial.ac.uk<mailto:d.gaboriau@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility Page: http://www.imperial.ac.uk/people/d.gaboriau

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[FILM-Users 00812] Cristina Lo Celso awarded RMS life science medal
by Bruckbauer, Andreas 01 Aug '18

01 Aug '18

Dear FILM microscope users, It is my pleasure to announce that Cristina Lo Celso has been awarded this year’s RMS medal for Life Sciences for outstanding scientific achievements applying microscopy in the field of cell biology. This is indeed very fitting as she will be taken over as head of the FILM facility from Tony next spring. Congratulations to Cristina! Please read the official announcement here: https://www.rms.org.uk/study-read/news-listing-page/rms-medal-series-2018-w… Best wishes Andreas ---------------------------------------------------- Dr Andreas Bruckbauer FILM facility manager Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 401 +44 (0)20 7594 2023 a.bruckbauer(a)imperial.ac.uk<mailto:a.bruckbauer@imperial.ac.uk> http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ ----------------------------------------------------

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[FILM-Users 00811] FW: FILM microscopy day 2018 - updated program
by Bruckbauer, Andreas 26 Jul '18

26 Jul '18

Please see email from Nicholas Mazarakis below and contact him directly if you can help. bw Andreas From: Mazarakis, Nicholas D FRSB Sent: 26 July 2018 19:33 To: Bruckbauer, Andreas <a.bruckbauer(a)imperial.ac.uk> Subject: RE: FILM microscopy day 2018 - updated program Hi Andreas I wonder if any user has a clean aliquot of the BV-2 mouse microglial cell line and can make it available to us. Thank you. BW Nicholas Professor Nicholas D. Mazarakis B.Sc., Ph.D., FRSB Lucas-Lee Chair of Molecular BioMedicine Head of Gene Therapy Centre for Neuroinflammation & Neurodegeneration Division of Brain Sciences Faculty of Medicine Imperial College London Hammersmith Hospital Campus E402 Burlington Danes Building Du Cane Road London W12 0NN Tel: +44 (0)20 7594 7024 Fax: +44 (0)20 7594 3200 Email:n.mazarakis@imperial.ac.uk<https://exchange.imperial.ac.uk/owa/UrlBlockedError.aspx> http://www1.imperial.ac.uk/medicine/people/n.mazarakis/

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