- FILM-Users - imperial.ac.uk

[FILM-Users 00790] !!!!!!!!!!!!!!!!!!!!!!!!!! Deletion of Images on Scratch !!!!!!!!!!!!!!!!!!!!!!!!!!!
by Rothery, Stephen M 15 Mar '18

15 Mar '18

Dear All The Scratch drive is getting full again so we need to delete some of the older files So on Monday we will be deleting everything dated to before 1st December 2017 Please make sure you have backed up your data Bw steve FILM - Facility for Imaging by Light Microscopy ************************************************************** Steve Rothery FILM Technician Sir Alexander Fleming Building, desk 403 Imperial College London / South Kensington Exhibition Road London SW7 2AZ Tel +44-(0)207 594 9793 And ICTEM, (L-Block) Room 314 Hammersmith Campus Imperial College London Du Cane Road London, W12 0NN Tel. +44-(0)207 594 1669 E-mail s.rothery(a)imperial.ac.uk<mailto:s.rothery@imperial.ac.uk> Website: http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ **************************************************************

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[FILM-Users 00789] Re: FILM/FLOW user meeting
by Bruckbauer, Andreas 08 Mar '18

08 Mar '18

Dear all, Steve will be talking about: FILM's FIJI Macros - using FIJI for image presentation and simple analysis At our combined FILM/FLOW user meeting on Wednesday the 28th of March from 12:45 - 2pm. the talk will be approx. 30 min and we have 15 min to discuss and get general feedback for FIM users Best wishes Andreas ---------------------------------------------------- Dr Andreas Bruckbauer FILM facility manager Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 401 +44 (0)20 7594 2023 a.bruckbauer(a)imperial.ac.uk<mailto:a.bruckbauer@imperial.ac.uk> http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ ---------------------------------------------------- From: Bruckbauer, Andreas Sent: 05 March 2018 10:46 To: film-users <film-users(a)imperial.ac.uk> Cc: Srivastava, Jane <j.srivastava(a)imperial.ac.uk> Subject: FILM/FLOW user meeting Good morning, The FILM and Flow facilities will be having a combined user meeting on Wednesday the 28th of March from 12:45 - 2pm. FILM will be hosting the first 45 minutes and us the second, you can choose whether you come to one or both and both sessions will be in the following format: * 15 minute presentation by the facility. * A chance for users to discuss problems or helpful hints from their own data. * Feedback for the facility. We hope to have this meeting every three months or so, and if you would like a chance to present your work in the presentation slot please contact me. If you would like to come please let us know for room size purposes. Best wishes, Andreas ---------------------------------------------------- Dr Andreas Bruckbauer FILM facility manager Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 401 +44 (0)20 7594 2023 a.bruckbauer(a)imperial.ac.uk<mailto:a.bruckbauer@imperial.ac.uk> http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ ----------------------------------------------------

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[FILM-Users 00788] FILM/FLOW user meeting
by Bruckbauer, Andreas 05 Mar '18

05 Mar '18

Good morning, The FILM and Flow facilities will be having a combined user meeting on Wednesday the 28th of March from 12:45 - 2pm. FILM will be hosting the first 45 minutes and us the second, you can choose whether you come to one or both and both sessions will be in the following format: * 15 minute presentation by the facility. * A chance for users to discuss problems or helpful hints from their own data. * Feedback for the facility. We hope to have this meeting every three months or so, and if you would like a chance to present your work in the presentation slot please contact me. If you would like to come please let us know for room size purposes. Best wishes, Andreas ---------------------------------------------------- Dr Andreas Bruckbauer FILM facility manager Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 401 +44 (0)20 7594 2023 a.bruckbauer(a)imperial.ac.uk<mailto:a.bruckbauer@imperial.ac.uk> http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ ----------------------------------------------------

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[FILM-Users 00787] THE ART OF RESEARCH 2018
by Rothery, Stephen M 28 Feb '18

28 Feb '18

Dear Postdocs & Students, THE ART OF RESEARCH 2018 The Art of research is back once again and seeking images that demonstrate the breadth and depth of scientific research taking place at Imperial College London! We're looking for photographs, illustrations, infographics, and whatever else you have up your sleeve that tells the story of your work. Whether it's a micrograph of some cells, a drawing of a drone, or an illustration of fluid dynamics, we're looking for original and creative images that celebrate science at its best. This year we're working with the Imperial Festival to hold the final exhibition at the Festival itself! This means that shortlisted entries will be displayed at the outreach event on the 28th and 29th of April to the visiting crowds. In 2017 more than 15,000 public and alumni visitors descended on Imperial's South Kensington campus. That's 15,000 people admiring your art! http://inno.im/artofresearch You can win amazing prizes! This year there will be two categories: the Judges' Choice and the People's Choice. Judges' Choice * An Apple iPad * A Destek V4 smartphone VR Headset * 3D Stereoscopic Lens adaptor for smartphones People's Choice * A Destek V4 smartphone VR Headset * 3D Stereoscopic Lens adaptor for smartphones Entrants whose submission is shortlisted for the final exhibition will also receive a print of their work, so remember: send us the highest resolution you have so your pièce de résistance can look its best! The contest is open to all staff and students at Imperial and will run until the 23th of March. You can find more information about the competition, prizes, judging and the Imperial Festival here: http://inno.im/artofresearch ALBA MARTIN QUICKTECH ASSOCIATE D +44 20 3053 8857<tel:+44%2020%203053%208857> [cid:image909000.jpg@7F15B3A1.4A5A024C]<http://imperialinnovations.co.uk/> Imperial Innovations Limited , 52 Princes Gate, Exhibition Road, London SW7 2PG T +44 (0)20 3053 8850<tel:+44%20(0)20%203053%208850> | imperialinnovations.co.uk<http://imperialinnovations.co.uk> Anita Alvarez Laviada, PhD, MSc Postdoctoral Research Associate Postdoc Rep - Hammersmith Campus National Heart & Lung Institute Dept. of Myocardial Function ICTEM Building, Level 4, Lab 412 Imperial College London Du Cane Road London, W12 0NN E-mail: a.alvarez-laviada(a)ic.ac.uk<mailto:a.alvarez-laviada@ic.ac.uk> Web: http://www.imperial.ac.uk/people/a.alvarez-laviada Tel: 07719 913 041 FILM - Facility for Imaging by Light Microscopy ************************************************************** Steve Rothery FILM Technician Sir Alexander Fleming Building, desk 403 Imperial College London / South Kensington Exhibition Road London SW7 2AZ Tel +44-(0)207 594 9793 And ICTEM, (L-Block) Room 314 Hammersmith Campus Imperial College London Du Cane Road London, W12 0NN Tel. +44-(0)207 594 1669 E-mail s.rothery(a)imperial.ac.uk<mailto:s.rothery@imperial.ac.uk> Website: http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ **************************************************************

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[FILM-Users 00786] CellProfiler survey
by Bruckbauer, Andreas 26 Feb '18

26 Feb '18

for anyone interested: http://broad.io/ImagingSurvey (see below) Andreas ---------- Forwarded message ---------- From: Beth Cimini <bcimini(a)broadinstitute.org<mailto:bcimini@broadinstitute.org>> Date: Fri, Feb 16, 2018 at 6:08 PM Subject: Quick survey for any and all image users To: CONFOCALMICROSCOPY(a)lists.umn.edu<mailto:CONFOCALMICROSCOPY@lists.umn.edu> ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hi all, I'm a member of the team at CellProfiler; we spend a lot of time thinking how best to help and do image analysis outreach to scientists from all fields and all experience levels- we'd love your input on a 10 minute survey to help us figure out who really wants and needs our help and how best to deliver it! Let us know if you want more video tutorials, workshops at conferences, or something else entirely- this is your chance to push the direction of our outreach! If you can signal-boost this to other users at your home institutions as well, we'd really appreciate it- we'd love to catch a wide net and reach as many users as possible, especially casual users who may not do enough imaging to have joined the listserv. http://broad.io/ImagingSurvey -- Beth Cimini, PhD Computational Biologist, Imaging Platform Broad Institute 415 Main St Room 5011 Cambridge, MA 02142 ---------------------------------------------------- Dr Andreas Bruckbauer FILM facility manager Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 401 +44 (0)20 7594 2023 a.bruckbauer(a)imperial.ac.uk<mailto:a.bruckbauer@imperial.ac.uk> http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ ----------------------------------------------------

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[FILM-Users 00785] Service contracts for Zeiss microscopes at Imperial
by Bruckbauer, Andreas 22 Feb '18

22 Feb '18

Dear PIs and lab managers, I now got confirmation from Zeiss that they will offer a 20% discount on service contracts for all of Imperial. There will not be a 2% early payment discount, but this is better as the 15% + 2% they were previously offering. So we don't need to pool instruments any more. Please contact Jatin at Zeiss if you want more information: Mr Jatin Ladha Account Manager - Widefield Carl Zeiss Limited Division of Microscopy ZEISS Group 509 Coldhams Lane Cambridge CB1 3JS United Kingdom Customer Care Team: +44 (0) 1223 401540 Fax: +44 (0) 1223 401501 jatin.ladha(a)zeiss.com<mailto:jatin.ladha@zeiss.com> http://www.zeiss.com/uk best wishes Andreas ---------------------------------------------------- Dr Andreas Bruckbauer FILM facility manager Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 401 +44 (0)20 7594 2023 a.bruckbauer(a)imperial.ac.uk<mailto:a.bruckbauer@imperial.ac.uk> http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ ----------------------------------------------------

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[FILM-Users 00784] Zeiss microscopes under service contract at Imperial
by Bruckbauer, Andreas 16 Feb '18

16 Feb '18

Dear PIs and lab managers, Please contact me If you have a Zeiss microscope on an annual service contract, we currently pool service contracts for 6 Zeiss microscopes and get a 13% discount from Zeiss by doing this. We could add more microscopes based at Imperial to this contract at 13% discount rate, saving you some money! If we would add two additional microscopes, the discount might increase to 15% saving us and you some money. Please let me know if you have anything to cover (sorry, old LSM 510 and Axiovert 200 will not be covered by Zeiss any more). Best wishes Andreas ---------------------------------------------------- Dr Andreas Bruckbauer FILM facility manager Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 401 +44 (0)20 7594 2023 a.bruckbauer(a)imperial.ac.uk<mailto:a.bruckbauer@imperial.ac.uk> http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ ----------------------------------------------------

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[FILM-Users 00783] Imaris license for interested groups
by Bruckbauer, Andreas 13 Feb '18

13 Feb '18

Dear all, We currently don't offer Imaris data analysis software for FILM users, but it is a very powerful and easy to use software package for 3D and 4D data analysis 3D rendering, segmentation, tracking and much more. There are a few groups at Imperial who consider to buy a license and share the costs, is anyone else willing to contribute? the more groups participate, the lower the cost for each group. Please contact me directly if you are interested. Best wishes Andreas ---------------------------------------------------- Dr Andreas Bruckbauer FILM facility manager Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 401 +44 (0)20 7594 2023 a.bruckbauer(a)imperial.ac.uk<mailto:a.bruckbauer@imperial.ac.uk> http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ ----------------------------------------------------

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[FILM-Users 00782] Image and Science Writing Competitions
by Bruckbauer, Andreas 06 Feb '18

06 Feb '18

Please see the Image competition below: -----Original Message----- From: Andrew Carter [mailto:cartera@mrc-lmb.cam.ac.uk] Sent: 06 February 2018 15:55 To: Braga, Vania M M <v.braga(a)imperial.ac.uk<mailto:v.braga@imperial.ac.uk>> Subject: BSCB Ambassadors: Image and Science Writing Competitions Dear Vania Braga, Could I ask you to encourage people at your Institution to submit an entry to our Image and/or Science Writing Competitions? We are a bit low on applications and could really do with some more! The deadline for both competitions is February 14th. Imaging Competition: Open to all BSCB members. Submit your best Cell Biology image. Prizes: 1st Prize £200, 2nd Prize £100, 3rd Prize £50, Details on how to apply: http://bscb.org/competitions-awardsgrants/image-competition/image-competiti… Previous winners/examples: http://bscb.org/competitions-awardsgrants/image-competition Science Writing Competition: Open to BSCB PhD student and Postdoc members: Articles of no more than 1000 words covering topics of key relevance to cell biology. 1st prize: £500, Details on how to apply: http://bscb.org/writing-competition-rules/ More information: http://bscb.org/competitions-awardsgrants/science-writing-prize/ Many thanks for your help. With best wishes Andrew Carter BSCB Membership secretary.

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[FILM-Users 00781] Important FILM news
by Bruckbauer, Andreas 01 Feb '18

01 Feb '18

Dear FILM Users, At the start of a new year I wanted to update you on a couple of important matters. First the good news! - we have ordered a new Leica SP8 confocal microscope with HyD detectors to replace the ageing Zeiss LSM510 (CF5) which is no longer supported by Zeiss. The new confocal will be installed in room 410. which is currently being refurbished as a CL2 room to image live and low risk infectious material (after consultation with Health &Safety). This room will also be equipped with a biological safety cabinet to handle the CL2 samples, and hand wash facilities. The time frame is roughly two month from now. Once the SP8 is up and running, the old 510 will be decommissioned. The FILM Team will assist anyone who needs to transfer protocols over from the 510 to the SP8. The new SP8 will have the following capabilities: - Four spectral detectors, two of them sensitive HyD detectors - 405, 457, 488, 515, 561, 594, 633 laser lines - Normal and resonance scanner - Motorised stage - Range of objectives from 10x to 63x oil and 40x water - DIC imaging - Incubation chamber The not so good news - the new confocal has to be paid for! As many of you know, FILM functions as a College "Small Research Facility" and as such we are required to recover our costs annually. These costs cover equipment maintenance contracts, salaries of FILM staff (about 50% of these are covered on grants) and a modest running budget. Each year around this time we set our charges at rates which we predict will allow us to recover those costs, based on predicted usage of our equipment and services. That predicted usage is informed by our 11 years of experience of running FILM, so we're not bad at it by now. However, inevitably this is not an exact science but we just aim to get as close as possible to our predicted spend. We do not try to make any "profit" from usage - you are just paying the real running costs of the facility. In recent years when we have had minor underspends, i.e. when usage has been significantly higher than we predicted, our host Department NHLI has allowed us to buy two new widefield instruments (WF1 and WF3) to replace old instruments. These have significantly improved our WF capability, to your benefit. In the last few years, again because of good usage, we have been able to hold our prices or in some years actually reduce them, again to your benefit. The more you use the 'scopes, the cheaper they will get per hour! Replacing large pieces of kit like a confocal is a challenge! In previous years we have had some success with grant applications to BBSRC and Wellcome Trust that have allowed us to buy our high content system (WF4-HC) and superresolution (SR1) microscopes. However, in the last couple of years we have had two unsuccessful bids to replace our confocal. The strong impression we get is that equipment "replacement" is disfavoured relative to buying "new technology" and we are unlikely to be successful by this route. So, to get our new desperately needed confocal we have had to be creative and NHLI has bought this instrument on the basis that it's cost is paid back over 5 years. That means that we have had to increase our charges slightly this year to raise the extra income to cover the instrument purchase. The charges have changed from: Confocal p.h. £32 in 2017 to £35 in 2018 Widefield p.h. £20 in 2017 to £21 in 2018 Confocal with MP laser p.h. £50 2017 to £53 in 2018 Software p.h. £6 in 2017 stays at £6 in 2018 Staff time p.h. £33 in 2017 reduced to £29 in 2018 Charges will increase from Feb 1st We hope that you recognise the importance of this purchase and the improved capability it will give you, and accept the modest price increases. Rest assured that we will always try to keep our costs down as much as possible while providing you with the excellent service you've come to expect and receive. Please contact Andreas if you have any questions or concerns. Many thanks for your understanding. Best regards and good imaging! Tony and Andreas ---------------------------------------------------- Dr Andreas Bruckbauer FILM facility manager Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 401 +44 (0)20 7594 2023 a.bruckbauer(a)imperial.ac.uk<mailto:a.bruckbauer@imperial.ac.uk> http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ ----------------------------------------------------

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