- FILM-Users - imperial.ac.uk

[FILM-Users 00249] change to Sharepoint booings
by Martin Spitaler 12 Sep '12

12 Sep '12

Dear microscopists, there was a slight change to the way new bookings are made: The field USER EMAIL is now selected from registered FILM users, rather than the college directory. Unfortunately, due to a technical glitch this field can be left empty, so we would need to go through all those bookings and enter it manually before recharging. So please make sure you select your own email address from the list for all your new bookings. It would also be very helpful if you could update all your recent bookings (in the last two weeks) if needed. Thanks, good imaging, Martin -- ------------------------------------------------------------------------ *Martin Spitaler, PhD* *FILM - Facility for Imaging by Light Microscopy* - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk <mailto:m.spitaler@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

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[FILM-Users 00248] Fwd: In Vivo Imaging Senior Scientific Officer position available
by Martin Spitaler 12 Sep '12

12 Sep '12

...forwarded, for your information, Martin -------- Original Message -------- Subject: In Vivo Imaging Senior Scientific Officer position available Date: Wed, 12 Sep 2012 11:13:12 +0100 From: Francois Lassailly <Francois.Lassailly(a)CANCER.ORG.UK> Reply-To: Francois Lassailly <Francois.Lassailly(a)CANCER.ORG.UK> To: <UK-EUROBIOIMAGING-PROJECT(a)JISCMAIL.AC.UK> Dear friends and colleagues, Please find bellow a brief description of the Senior Scientific Officer position available at the LRI In Vivo Imaging Facility. I would be very grateful if you could follow the information to potential good candidates you might think of. Best wishes, Francois Lassailly *Francois Lassailly, MSc PhD,* *---------------------------------------------------------------------*** *In Vivo Imaging Facility*** *London Research Institute*** *Cancer Research UK (RCN 1089464)* *44 Lincoln's Inn Fields* *London WC2A 3PX - United Kingdom* *Tel : +44 (0) 20 7269 3426* *Fax : +44(0) 20 7269 3581* *Email : francois.lassailly(a)cancer.org.uk* *Website: http://www.london-research-institute.org.uk/technologies/in-vivo-imaging* *UK-EUROBIOIMAGING Visit the website http://www.bioimaginguk.org <http://www.bioimaginguk.org/>* *European Society for Molecular Imaging: www.e-smi.eu* *---------------------------------------------------------------------* Senior Scientific Officer, In Vivo Imaging Facility We are looking for a Senior Scientific Officer in the In Vivo Imaging core facility at the London Research Institute (LRI). The facility is equipped with Biophotonic / Optical whole body imaging (Bioluminescence (BLI), Fluorescence, including NIR, spectral unmixing, tomography), X-ray microCT as well as combined multiphoton / confocal microscopy for intravital imaging of live tissues with a sub-cellular resolution. To support our research activity, you will be a committed and proactive team member with interest in science and management. The role will involve evaluating and validating the use of optical and radionuclide probes and / or micro-CT imaging in order to monitor disease progression during longitudinal studies. You will be involved in all aspects of the study ranging from design, realisationas well as analysis and presentation of theresults for internal and external communications. For more information please visit the page bellow: *https://cruk.taleo.net/careersection/cruk_corporate/jobdetail.ftl?lang=en&job=SCI00246* * * * * * * NOTICE AND DISCLAIMER This e-mail (including any attachments) is intended for the above-named person(s). If you are not the intended recipient, notify the sender immediately, delete this email from your system and do not disclose or use for any purpose. We may monitor all incoming and outgoing emails in line with current legislation. We have taken steps to ensure that this email and attachments are free from any virus, but it remains your responsibility to ensure that viruses do not adversely affect you. Cancer Research UK Registered charity in England and Wales (1089464), Scotland (SC041666) and the Isle of Man (1103) A company limited by guarantee. Registered company in England and Wales (4325234) and the Isle of Man (5713F). Registered Office Address: Angel Building, 407 St John Street, London EC1V 4AD. -- UK-EUROBIOIMAGING Visit the website http://www.bioimaginguk.org To e-mail the group, send messages to UK-EUROBIOIMAGING-PROJECT(a)JISCMAIL.AC.UK To subscribe visit https://www.jiscmail.ac.uk/cgi-bin/webadmin?A0=UK-EUROBIOIMAGING-PROJECT --

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[FILM-Users 00247] problems with image acquisition due to computer / software problems
by Martin Spitaler 06 Sep '12

06 Sep '12

Dear microscopists, unfortunately we all know that problems with software crashes during image acquisition are far too frequent, and with time naturally computers get older, which makes things worse. We are trying to minimise this by regularly cleaning up and defragmenting the harddrives, but after years of heavy usage this can't completely solve the problem. Consequently, I have asked Leica to replace the acquisition computers with new up-to-date machines, which I thought should be a routine thing having everything under a GOLD PLUS contract, claiming to keep each microscope "checked to its original performance specifications" and including "spare part cover". Unfortunately, Leica see it differently and insist on charging for a "computer upgrade", and no less than £9,000 for two systems (CF3 and CF4). I'm sure you will agree with me that it is not something unexpected that a computer gets slower after six years of heavy usage and should be regarded like any other ageing spare part. One important aspect of the whole discussion is that a slow computer does indeed affect the performance of the system, either slowing down image acquisition, limiting the ways multi-dimensional datasets can be acquired and probably most dramatically resulting in more frequent software crashes, with all their effects on your time and live samples. To help me with my argument, I'm asking for your help here: (1) Could ALL of you (including users of other microscopes - the same discussion will come up with other manufacturers) please write down until end of September EVERY TIME an error code or have to restart the software, or any other computer-related problem. Please use the normal NOTES AND TROUBLES field in the SHAREPOINT BOOKING for this. I know on some microscopes it's so frequent that you don't bother anymore, but please let's create an accurate record of these problems for a few weeks. (2) Those of you who affected, could you please send me a short written feedback describing how unstable systems (due to computer / software problems) have affected you, either as a direct financial loss (e.g. lost time and samples, expensive stainings, ...) or just as a constant hindrance to your work; while the former is a direct argument, the latter will decide future purchasing decisions, so could be convincing as well. I hope with your help we will manage to convince Leica (and later others) to simply care more for the users of their microscopes. If successful, it could save you as facility users a lot of money as well, £9,000 corresponds to more than 200 hours of usage; and while we don't charge for usage hours when microscopes don't work, it reduces the number of usable hours, driving up the rates. Thanks, good imaging, Martin -- ------------------------------------------------------------------------ *Martin Spitaler, PhD* *FILM - Facility for Imaging by Light Microscopy* - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk <mailto:m.spitaler@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

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[FILM-Users 00246] CO2 incubator
by Rothery, Stephen M 30 Aug '12

30 Aug '12

Dear all The CO2 incubator in the widefield room, SAF has developed a fault It is reading 21% CO2 which does not change even with the door open I suggest it is not used until it is sorted Steve FILM - Facility for Imaging by Light Microscopy ************************************************************** Steve Rothery FILM Technician Sir Alexander Fleming Building, desk 403 Imperial College London / South Kensington Exhibition Road London SW7 2AZ Tel +44-(0)207 594 9793 And ICTEM, (L-Block) Room 314 Hammersmith Campus Imperial College London Du Cane Road London, W12 0NN Tel. +44-(0)207 594 1699 E-mail s.rothery(a)imperial.ac.uk<mailto:s.rothery@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility **************************************************************

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[FILM-Users 00245] Fwd: Invitation to the new LIVIm club
by Martin Spitaler 13 Aug '12

13 Aug '12

...forwarded for your information; the attachments can be found on the FILM scratch disk under <x:\Mailing list attachments\>, Martin -------- Original Message -------- Subject: Invitation to the new LIVIm club Date: Mon, 6 Aug 2012 14:30:22 +0100 From: LIVIm <LIVIm(a)cancer.org.uk> To: LIVIm <LIVIm(a)cancer.org.uk> Dear friends and colleagues This is my pleasure to invite you to the launching of the *LRI /In Vivo/ Imaging (LIVIm) club* on *Monday September 24^th at 17:30*. The main focus of LIVIm is to enable multidisciplinary exchanges and collaborations between the numerous actors of this rapidly growing field. This initiative should cover all aspects of /in vivo/ imaging from the whole body to the sub-cellular scale, in animal models and in humans. Attached is the program of the first meeting as well as a short presentation of LIVIm aims and objectives. Drinks and munchies will be provided after the talks to favour interactions between the participants. *Registration is _FREE_ but _REQUIRED_, please email your details at LIVIm(a)cancer.org.uk <mailto:LIVIm@cancer.org.uk>.* *Mailing list*: if you wish to receive programs for future meetings and announcements, simply email *LIVIm(a)cancer.org.uk <mailto:LIVIm@cancer.org.uk>* with “*SUBSCRIBE*” in the object of your message. Inversely if you do not wish to receive further emails please put “*UNSUBSCRIBE*” in the object of your message. I hope you will enjoy these very exciting talks and found the experience instructive and useful. Looking forward to seeing you there around great science! * * Best wishes, Francois Lassailly * Francois Lassailly, MSc PhD, --------------------------------------------------------------------- In Vivo Imaging Facility London Research Institute Cancer Research UK (RCN 1089464) 44 Lincoln's Inn Fields London WC2A 3PX - United Kingdom Tel : +44 (0) 20 7269 3426 Fax : +44(0) 20 7269 3581 Email : LIVIm(a)cancer.org.uk <mailto:LIVIm@cancer.org.uk>* *LIVIm: **http://www.london-research-institute.org.uk/news-and-events/special-interest-groups#invivo* *In Vivo Imaging facility: http://www.london-research-institute.org.uk/technologies/in-vivo-imaging* *---------------------------------------------------------------------* NOTICE AND DISCLAIMER This e-mail (including any attachments) is intended for the above-named person(s). If you are not the intended recipient, notify the sender immediately, delete this email from your system and do not disclose or use for any purpose. We may monitor all incoming and outgoing emails in line with current legislation. We have taken steps to ensure that this email and attachments are free from any virus, but it remains your responsibility to ensure that viruses do not adversely affect you. Cancer Research UK Registered charity in England and Wales (1089464), Scotland (SC041666) and the Isle of Man (1103) A company limited by guarantee. Registered company in England and Wales (4325234) and the Isle of Man (5713F). Registered Office Address: Angel Building, 407 St John Street, London EC1V 4AD.

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[FILM-Users 00244] Fwd: Reagent request
by Martin Spitaler 13 Aug '12

13 Aug '12

...forwarded: can anyone help? If so, please contact Natalie directly, Martin -------- Original Message -------- Subject: Reagent request Date: Mon, 13 Aug 2012 10:02:22 +0100 From: Welsh, Natalie <natalie.welsh07(a)imperial.ac.uk> To: Spitaler, Martin <m.spitaler(a)imperial.ac.uk> Hi Martin, I'm looking for a small aliquot of Cell Mask Deep Red Membrane Stain to stain half a 96wp. Do you have any that I could use? If not, please could you send an email to FILM users to ask if anyone else has some? Many thanks, Natalie -- ------------------------------------------------------------------------ *Martin Spitaler, PhD* *FILM - Facility for Imaging by Light Microscopy* - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk <mailto:m.spitaler@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

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[FILM-Users 00243] Fwd: Stowers Original Data Repository
by Martin Spitaler 08 Aug '12

08 Aug '12

...forward for your information - I recommend you have a look at Stowers Repository, containing all ORIGINAL image data of all their publications in a freely accessible image viewer: Finally you can see for yourself what the microscopic images really looked like, and finally all those 'unspecific bands' on the Western Blots are back, that turned out to be the real story, years later... Martin -------- Original Message -------- Subject: Stowers Original Data Repository Date: Sun, 22 Jul 2012 17:39:22 +0000 From: Jason Swedlow <j.r.swedlow(a)DUNDEE.AC.UK> Reply-To: Jason Swedlow <j.r.swedlow(a)DUNDEE.AC.UK> To: <UK-EUROBIOIMAGING-PROJECT(a)JISCMAIL.AC.UK> Dear All- Many of you are thinking about mechanisms for archiving and releasing original data associated with scientific publications. Last week, the Stowers Institute announced their institutional repository for original data associated with their publications. The announcement is here: http://www.stowers.org/media/news/jul-13-2012 And the repository itself is here: http://srdr.stowers.org/ The Stowers ODR is based on Bio-Formats and OMERO, open source technology built by the OME Consortium (http://www.openmicroscopy.org/) The OME Consortium is supported by grants from the Wellcome Trust and the BBSRC. As you probably know, the JCB DataViewer (http://jcb-dataviewer.rupress.org <http://jcb-dataviewer.rupress.org/>) and ASCB CELL (http://cellimagelibrary.org <http://cellimagelibrary.org/>) are other examples of public image repositories based on OMERO and Bio-Formats. The OME Consortium is proud that its technology is helping make scientific data open and freely available. Cheers, Jason ---------------------- Wellcome Trust Centre for Gene Regulation & Expression | Open Microscopy Environment | University of Dundee Phone: +44 (0) 1382 385819 email: j.swedlow(a)dundee.ac.uk <mailto:j.swedlow@dundee.ac.uk> Web: http://www.lifesci.dundee.ac.uk/gre/staff/jason-swedlow Open Microscopy Environment: http://openmicroscopy.org The University of Dundee is a registered Scottish Charity, No: SC015096 -- UK-EUROBIOIMAGING Visit the website http://www.bioimaginguk.org To e-mail the group, send messages to UK-EUROBIOIMAGING-PROJECT(a)JISCMAIL.AC.UK To subscribe visit https://www.jiscmail.ac.uk/cgi-bin/webadmin?A0=UK-EUROBIOIMAGING-PROJECT --

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[FILM-Users 00242] virtual nanoscopy: whole zebrafish at nm resolution
by Martin Spitaler 08 Aug '12

08 Aug '12

Dear microscopists, for those of you not working on zebrafish who might have missed it: A recent publication in the Journal of Cell Biology has combined electron microscopy and clever data management to produce a gigapixel image of a whole zebrafish embryo at 2.5nm resolution, allowing navigation from the whole organism to subcellular structures in Google Maps style. Image: <http://v.jcb-dataviewer.glencoesoftware.com/webclient/img_detail/201/> Article: <http://jcb.rupress.org/content/198/3/457.full> This is so impressive, like a preview of things to come in microscopy, that I thought it's worth drawing your attention to it. Enjoy! Good imaging, Martin PS: the JCB Data Viewer is based on Omero, which we also use and recommend in FILM for image data storage and annotation -- ------------------------------------------------------------------------ *Martin Spitaler, PhD* *FILM - Facility for Imaging by Light Microscopy* - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk <mailto:m.spitaler@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

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[FILM-Users 00241] Fwd: Flow cytometry course- London
by Martin Spitaler 07 Aug '12

07 Aug '12

...forwarded, for your information; not directly related to microscopy, but looks like a good course, and will be interesting to many of you, Martin -------- Original Message -------- Subject: Flow cytometry course- London Date: Thu, 19 Jul 2012 04:09:02 -0600 From: Flowise Scientific <events(a)regonline.co.uk> Reply-To: Flowise Scientific <info(a)flowise-scientific.com> To: <m.spitaler(a)imperial.ac.uk> hi *Registration* in now open for our next flow cytometry course/meeting. Applications of flow cytometry course will be held on*24^th Oct 2012* in London-UK. We have an excellent line-up of speakers including: *Derek Davies -Mike Ormerod -Mark Cheetham -Richard Grenfell Niga Nawroly -Rachael Walker - Andy Filby- and Esperanza Perucha* Join us to learn about flow cytometry and its applications. Discuss issues, learn troubleshoot. Registration is open now, please click here: http://www.regonline.co.uk/Register/Checkin.aspx?EventID=1078303 Program <https://www.regonline.co.uk/custImages/343128/Applicationsofflowcytometryfl…> *Course Fees:* •£99 Student • £120 Other • 10% discount- Group registration (5 of more) Details of the program found here: *http://flowise-scientific.com/?page_id=38*** Please feel free to distribute *Beast Regards Khoshy Event Coordinator info(a)flowise-scientific.com *flowise-scientific.com This email communication was sent by: Flowise Scientific To unsubscribe, click here <https://www.regonline.co.uk/t/u.aspx?e=9&p=1311199&a=dWAyhIaWisMrjmcFygqEqw…>. View our Privacy Policy <https://www.regonline.co.uk/Privacy.aspx>

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[FILM-Users 00240] Fwd: Media Cybernetics imaging day
by Martin Spitaler 07 Aug '12

07 Aug '12

...forwarded, for your information, Martin -------- Original Message -------- Subject: Media Cybernetics imaging day Date: Mon, 16 Jul 2012 10:04:34 +0000 From: James McKechnie <jmckechnie(a)mediacy.com> To: m.spitaler(a)imperial.ac.uk <m.spitaler(a)imperial.ac.uk> I would like to invite you to a day of hands on sessions and talks to explore some of the best ways to get more from the equipment that you have and to look at the latest advances in the market. This will be between 10am and 4:30pm on the 9th of August and will be held in out training facility at Moor Hall, Cookham, Berkshire. We will be running 4 sessions from throughout the day and there will be talks covering the following topics; with a choice of 2 during each session. These are stand-alone topics and so you will be able to pick and chose the topicsthat interest you the most. All of the sessions will include information and hands on experience with the equipment. - Analysis and Counting - Capturing large areas of sample with a high magnification objective - Advances in colour analysis techniques - Simplifying workflows and complex analysis - Analysing moving or changing objects - Camera testing and technology Lunch and all refreshments throughout the day will be provided. To register your interest and for more information please email jmckechnie(a)mediacy.com James McKechnie Account Manager Media Cybernetics UK A Division of Roper Industries Ltd Beech House 27 Little Marlow Road Marlow SL7 1HA Tel: +44(0)1628 477025 ext.103 Mobile: 07515 974896 Fax: +44(0)1628 891764 Email: jmckechnie(a)mediacy.com URL: www.mediacy.com -- ------------------------------------------------------------------------ *Martin Spitaler, PhD* *FILM - Facility for Imaging by Light Microscopy* - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk <mailto:m.spitaler@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

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