- FILM-Users - imperial.ac.uk

[FILM-Users 00282] Annual FILM report 2011-12
by Martin Spitaler 15 Nov '12

15 Nov '12

Dear FILM Users, Please find the annual FILM report 2011-12 on the FILM website under RESOURCES / DOCUMENTS (https://workspace.imperial.ac.uk/imagingfacility/Internal/FILM%20report%202…) Overall it’s been another good year for FILM in difficult financial circumstances. The establishment of the L block satellite has been one highlight. Please let me or Martin know if you have any comments or queries. Many thanks. And at this place a reminder: You may not be aware of the financial model FILM operates on – College requires us to recover /all/ our costs but /not/ to make a surplus. You can imagine this is not a straightforward challenge, but we do our best and so far have thankfully broken even or made only a slight surplus! To set our charges for the upcoming year, in essence what we do is divide our costs (which are more or less fixed – service contracts, consumables/minor equipment and salaries) by the number of hours usage of the microscopes and other services we offer. So you can see that this simple calculation means that the more the microscopes get used, the less we can charge for usage in the next year – so, the more you use FILM, the cheaper it gets! Best, Tony Anthony I. Magee Professor of Membrane Biology Head of Section of Molecular Medicine National Heart and Lung Institute Faculty of Medicine Imperial College London Sir Alexander Fleming Building Exhibition Road South Kensington London SW7 2AZ UK Tel. +44 (0)20 7594 3135 FAX +44 (0)20 7594 3015 http://www1.imperial.ac.uk/nhli/molecular/lipidation/ http://www1.imperial.ac.uk/medicine/people/t.magee/

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[FILM-Users 00281] Fwd: CONFOCALMICROSCOPY Index - 8 Nov 2012 to 9 Nov 2012 (#2012-267)
by Martin Spitaler 15 Nov '12

15 Nov '12

...forwarded for your information: This new ImageJ plugin could save us all a lot of time! Martin -------- Original Message -------- Subject: CONFOCALMICROSCOPY Index - 8 Nov 2012 to 9 Nov 2012 (#2012-267) Date: Sat, 10 Nov 2012 00:05:42 -0600 From: CONFOCALMICROSCOPY automatic digest system <LISTSERV(a)LISTS.UMN.EDU> Reply-To: <CONFOCALMICROSCOPY-Search-request(a)LISTS.UMN.EDU> To: <CONFOCALMICROSCOPY(a)LISTS.UMN.EDU> *Subject:* New ImageJ plugin for figure creation. *From:* Jerome Mutterer <jerome.mutterer(a)IBMP-ULP.U-STRASBG.FR> *Reply-To:* Confocal Microscopy List <CONFOCALMICROSCOPY(a)LISTS.UMN.EDU> *Date:* Fri, 9 Nov 2012 15:45:42 +0100 *Content-Type:* text/plain ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Dear all, Today, we are releasing the first public beta version of FigureJ, an ImageJ plugin that will help you build articles figures from your images. It reduces the time needed to build a figure from hours to minutes, works with the standard ImageJ release, and adds some very interesting features like it keeps track of original datasource and processing. Adding scalebars for example is made very easy. Figure annotation can be done with ImageJ built-in tools. Check out the videos on the ImageJ documentation wiki to see how simple it is (http://goo.gl/yIzq3) <http://goo.gl/yIzq3%29.> This is the first public version, so it certainly has bugs in it, and the code is not finished. Of course it's free and open-source, so everyone is welcome to suggest or contribute enhancements. We acknowledge the support of the Institute for Plant Molecular Biology, CNRS , Strasbourg, France; HTW Berlin, Germany, and Carl Zeiss. All comments are welcome. Sincerely, Jerome. http://imagejdocu.tudor.lu/doku.php?id=plugin:utilities:figurej:start CONFOCALMICROSCOPY Index - 8 Nov 2012 to 9 Nov 2012 (#2012-267) Browse the CONFOCALMICROSCOPY online archives. <http://lists.umn.edu/cgi-bin/wa?LIST=CONFOCALMICROSCOPY> Powered by the LISTSERV Email List Manager <http://www.lsoft.com/products/listserv-powered.asp>

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[FILM-Users 00280] Fwd: [neuroscience-technology-network] Carandini Lecture Mon 5pm, UCL
by Martin Spitaler 08 Nov '12

08 Nov '12

...forwarded for your information. The original flyer is on the FILM documentation server at <\\film-nas.med.ic.ac.uk\documentation\film-user mailing list\121112 Matteo Carandini Lecture.pdf> Sainsbury Wellcome Centre Lecture Series on Neural Circuits & Behaviour Professor Matteo Carandini "Probing spatial computations in mouse visual cortex" MONDAY 12th NOVEMBER 5pm AV HILL LECTURE THEATRE HOST: John O'Keefe Please join us for a glass of wine afterwards Starling Room- Medical Sciences Room 250 -------- Original Message -------- Subject: [neuroscience-technology-network] Carandini Lecture Mon 5pm, UCL Date: Thu, 8 Nov 2012 13:15:44 +0000 From: Simon Schultz <s.schultz(a)imperial.ac.uk> Reply-To: <s.schultz(a)imperial.ac.uk> To: neuroscience-technology-network <neuroscience-technology-network(a)imperial.ac.uk> Dear all, Please see attached as a PDF details of a lecture by Matteo Carandini at UCL on monday. Cheers, Simon

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[FILM-Users 00279] Fwd: Re: Super-resolution symposia Thursday May 3rd LMCB 5pm
by Martin Spitaler 08 Nov '12

08 Nov '12

...forwarded for your information. The attachment is at <\\film-nas.med.ic.ac.uk\film-user mailing list\121206 Super-resolution Club.pptx> Martin -------- Original Message -------- Subject: Re: Super-resolution symposia Thursday May 3rd LMCB 5pm Date: Thu, 1 Nov 2012 13:54:35 +0000 From: Ann Wheeler <a.p.wheeler(a)qmul.ac.uk> To: Parsons, Maddy <maddy.parsons(a)kcl.ac.uk> CC: Nick Barry <nbarry(a)mrc-lmb.cam.ac.uk>, Daniel Metcalf <daniel.metcalf(a)npl.co.uk>, Ian Dobbie <ian.dobbie(a)bioch.ox.ac.uk>, "neveen hosny" <n.hosny(a)imperial.ac.uk>, Andrew Vaughan <andrew.vaughan(a)UCL.AC.UK>, "Cox, Susan" <susan.cox(a)kcl.ac.uk>, "Harris, John" <john.l.harris(a)kcl.ac.uk>, "French, Paul (PHOT) M W (Photonics)" <paul.french(a)imperial.ac.uk>, "Martin Knight" <m.m.knight(a)qmul.ac.uk>, Andrevi Sapelkin <a.sapelkin(a)qmul.ac.uk>, John Connelly <j.connelly(a)qmul.ac.uk>, <wen.wang(a)qmul.ac.uk>, <sunil.kumar(a)imperial.ac.uk>, <rainer.heintzmann(a)kcl.ac.uk>, <simon.ameer-beg(a)kcl.ac.uk>, <cartera(a)mrc-lmb.cam.ac.uk>, <anne.vaahtokari(a)cancer.org.uk>, Angray Kang <a.s.kang(a)qmul.ac.uk>, <alex.knight(a)npl.co.uk>, <ejr36(a)cam.ac.uk>, <meerdelyi(a)gmail.com>, "Jones, Gareth" <gareth.jones(a)kcl.ac.uk>, Martin Spitaler <m.spitaler(a)imperial.ac.uk>, Yan Gu <ygu(a)nimr.mrc.ac.uk>, Ricardo Henriques <paxcalpt(a)gmail.com>, Microscopy List <UK-MICROSCOPY(a)JISCMAIL.AC.UK>, "Wright, Colin" <colin.wright(a)nikon.co.uk>, "Khaliq, Ghazanfar" <ghazanfar.khaliq(a)nikon.co.uk> Dear All This is to let you know that the second meeting of the super-resolution club has been arranged for Thursday December 6th at the LMCB at UCL at 5pm. Dr Ricardo Henriques, Institute Pasteur, France. will give a talk titled: 'A molecular scale view into cells through super-resolution microscopy' Please do pass this message onto interested colleagues. Best wishes Ann Dr Ann Wheeler BALM Facility Manager Blizard Institute 4 Newark Street London E1 2AT Tel: 020 7882 2406

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[FILM-Users 00278] Fwd: FW: interesting seminar Monday November 12 - Alan Waggoner - Fluorescent biosensors and targeted physiological indicators for cell pathways
by Martin Spitaler 06 Nov '12

06 Nov '12

...forwarded for your information - should be a good seminar. As usual, the attachment (PDF) is on the FILM Documentation server under <\\film-nas.med.ic.ac.uk\documentation\film-user mailing list\121112 LSF Poster Waggoner London screen.pdf>, Martin -------- Original Message -------- Subject: FW: interesting seminar Monday November 12 - Alan Waggoner - Fluorescent biosensors and targeted physiological indicators for cell pathways Date: Mon, 5 Nov 2012 18:25:40 +0000 From: French, Paul (PHOT) M W (Photonics) <paul.french(a)imperial.ac.uk> To: Spitaler, Martin <m.spitaler(a)imperial.ac.uk> Dear Martin, Please could you email this invitation to an exciting imaging seminar -- with drinks reception - to the FILM mailing list and any other colleagues that you think might be interested. Alan Waggoner is one of the pioneers of fluorescence-based sensors and is currently working on new strategies for live cell imaging of cell pathways with small molecule dyes. All are welcome. Please can you forward the message topotentially interested colleagues. Best wishes, Paul November 12-14: "Fluorescent biosensors and targeted physiological indicators for cell pathways" Keynote Speaker Prof. Alan S. Waggoner Director of Molecular Biosensor and Imaging Center Department of Biological Sciences, Carnegie Mellon University, Pittsburgh / US Venues * *LSF London - Imperial College *Monday, November 12 -- 5:30pm. Chair: Prof. Paul French Venue: Imperial College London, Lecture Theatre 3, Blackett Laboratory, Prince Consort Road, London SW7 2BB Lecture Alan S. Waggoner: We develop fluorescent biosensors toolkits for pathways of living cells, focussing on smart biosensors for high throughput methods. Our biosensor platform consists of Fluorescence Activating Proteins (FAPs) that bind fluorogen dyes with large fluorescence increases. Sensors have been developed to rapidly and selectively track FAP labeled proteins such as GPCRs. This platform is proving promising for developing calcium, pH, sodium, potassium and redox sensors that will emit signals only from investigator-targeted regions of a cell. Sensors for protein-protein interactions and protein modification are in progress. Learn more about Alan S. Waggoner: <http://www.cmu.edu/bio/faculty/waggoner.html> Invitation The members of the Scientific Advisory Board UK - Prof. Paul French (Imperial College London), Prof. Antony Galione (Oxford Univ.), Dr. Jim Haseloff (Cambridge Univ.), Prof. David Klenerman (Cambridge Univ.), Prof. Gero Miesenboeck (Oxford Univ.), Prof. Tony Ng (King's College London), Dr. Violaine Sée, Dr. Bob Burgoyne, Prof. Alexei Tepikin (Liverpool Univ.), Prof. Mike White (Manchester Univ.), Prof. Keith Willison (Institute of Cancer Research London), Prof. Tony Wilson (Oxford Univ.), and Dr. Thomas Zapf (Leica Microsystems) - cordially invite you to meet Alan Waggoner at the post lecture receptions for personal discussion. Registration All lectures are free of charge. For organizational reasons please RSVP by e-mail to LSF(a)leica-microsystems.com <mailto:LSF@leica-microsystems.com>. For more information see <http://www.leica-microsystems.com/events/leica-scientific-forum/lsf-details…>

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[FILM-Users 00277] Fwd: FW: interesting seminar Monday November 12 - Alan Waggoner - Fluorescent biosensors and targeted physiological indicators for cell pathways
by Martin Spitaler 06 Nov '12

06 Nov '12

...forwarded for your information - should be a good seminar. As usual, the attachment (PDF) is on the FILM Documentation server under <\\film-nas.med.ic.ac.uk\documentation\film-user mailing list\121112 LSF Poster Waggoner London screen.pdf>, Martin -------- Original Message -------- Subject: FW: interesting seminar Monday November 12 - Alan Waggoner - Fluorescent biosensors and targeted physiological indicators for cell pathways Date: Mon, 5 Nov 2012 18:25:40 +0000 From: French, Paul (PHOT) M W (Photonics) <paul.french(a)imperial.ac.uk> To: Spitaler, Martin <m.spitaler(a)imperial.ac.uk> Dear Martin, Please could you email this invitation to an exciting imaging seminar -- with drinks reception - to the FILM mailing list and any other colleagues that you think might be interested. Alan Waggoner is one of the pioneers of fluorescence-based sensors and is currently working on new strategies for live cell imaging of cell pathways with small molecule dyes. All are welcome. Please can you forward the message topotentially interested colleagues. Best wishes, Paul November 12-14: "Fluorescent biosensors and targeted physiological indicators for cell pathways" Keynote Speaker Prof. Alan S. Waggoner Director of Molecular Biosensor and Imaging Center Department of Biological Sciences, Carnegie Mellon University, Pittsburgh / US Venues * *LSF London - Imperial College *Monday, November 12 -- 5:30pm. Chair: Prof. Paul French Venue: Imperial College London, Lecture Theatre 3, Blackett Laboratory, Prince Consort Road, London SW7 2BB * *LSF Oxford - University of Oxford *Tuesday, November 13 -- 4:00pm. Chair: Prof. Gero Miesenboeck Venue: Main Seminar Room, Biochemistry Department, South Parks Road, Oxford * *LSF Liverpool - Liverpool University *Wednesday, November 14 -- 5:00pm. Chair: Prof. Violaine Sée Venue: Lecture Theatre 3, Life Sciences Building, Crown Street, Liverpool L69 7ZB Lecture Alan S. Waggoner: We develop fluorescent biosensors toolkits for pathways of living cells, focussing on smart biosensors for high throughput methods. Our biosensor platform consists of Fluorescence Activating Proteins (FAPs) that bind fluorogen dyes with large fluorescence increases. Sensors have been developed to rapidly and selectively track FAP labeled proteins such as GPCRs. This platform is proving promising for developing calcium, pH, sodium, potassium and redox sensors that will emit signals only from investigator-targeted regions of a cell. Sensors for protein-protein interactions and protein modification are in progress. Learn more about Alan S. Waggoner: <http://www.cmu.edu/bio/faculty/waggoner.html> Invitation The members of the Scientific Advisory Board UK - Prof. Paul French (Imperial College London), Prof. Antony Galione (Oxford Univ.), Dr. Jim Haseloff (Cambridge Univ.), Prof. David Klenerman (Cambridge Univ.), Prof. Gero Miesenboeck (Oxford Univ.), Prof. Tony Ng (King's College London), Dr. Violaine Sée, Dr. Bob Burgoyne, Prof. Alexei Tepikin (Liverpool Univ.), Prof. Mike White (Manchester Univ.), Prof. Keith Willison (Institute of Cancer Research London), Prof. Tony Wilson (Oxford Univ.), and Dr. Thomas Zapf (Leica Microsystems) - cordially invite you to meet Alan Waggoner at the post lecture receptions for personal discussion. Registration All lectures are free of charge. For organizational reasons please RSVP by e-mail to LSF(a)leica-microsystems.com <mailto:LSF@leica-microsystems.com>. For more information see <http://www.leica-microsystems.com/events/leica-scientific-forum/lsf-details…>

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[FILM-Users 00276] Fwd: Fluo4-AM
by Martin Spitaler 29 Oct '12

29 Oct '12

...can anybody help Eric? Martin -------- Original Message -------- Subject: Fluo4-AM Date: Mon, 29 Oct 2012 16:15:44 +0000 From: Dubuis, Eric D <e.dubuis(a)imperial.ac.uk> To: Spitaler, Martin <m.spitaler(a)imperial.ac.uk> Hi Martin, Would you know of anybody who uses fluo4-AM and could spare some ? My stock has gone off and I need some for this week. 10 microgram wopuld be more than enough. Cheers Eric Dr Eric Dubuis Tel: + 44 (0) 207 594 8427 Fax: + 44 (0) 207 594 3100 Research Fellow in Airway Disease National Heart and Lung Institute Faculty of Medicine Sir Alexander Fleming Building South Kensington Campus http://www.imperial.ac.uk/collegedirectory/index.asp?PeopleID=496153

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[FILM-Users 00275] Fwd: formal post doc position
by Martin Spitaler 29 Oct '12

29 Oct '12

...forwarded for your information, Martin Subject: formal post doc position From: Alberto Diaspro <sekkio(a)MAC.COM> Reply-To: Confocal Microscopy List <CONFOCALMICROSCOPY(a)LISTS.UMN.EDU> Date: Sun, 28 Oct 2012 18:09:31 +0100 Content-Type: text/plain ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Dear friends, please consider and/or pass the info: Postdoc position on Molecular Dynamics Nanophysics Postdoctoral position on Molecular Dynamics Investigations for Sub-diffraction Imaging and Lithography Techniques by Means of the RESOLFT Concept Budget code 67788 The Fondazione Istituto Italiano di Tecnologia - IIT - was founded with the objective of promoting the country's technological development and further education in science and technology. In this sense, IIT's scientific program is based in the combination of the basic scientific research with the development of technical applications, a major inspirational principle. The research areas cover scientific topics of high innovative content, which represent the most advanced frontiers of modern technology, with wide application possibilities in various fields ranging from medicine to industry, from computer science to robotics, life sciences, and nanobiotechnology. Applications are invited for a Post-doctoral position on the study of molecular dynamics for sub-diffraction imaging and lithography techniques by means of the RESOLFT Concept. The project stems from a strong collaboration between the department of Nanophysics at the IIT in Genoa and the Center for Nano Science and Technology at the IIT @Polimi in Milan. The project aims to unveil the limitations in the switching mechanisms of the existing RESOLFT methods in order to propose innovative solutions, both in terms of ad hoc molecular systems and photophysical processes, for the design of cutting-edge nanoscopy set-ups. The candidate will be responsible both for the photophysical characterization of the active materials involved in the RESOLFT process by time resolved optical spectroscopy and for the design and realization of an optical setup to perform fluorescence imaging and lithographic measurements with sub-diffraction resolution. The applicant should have a PhD in physics and/or engineering with good knowledge of physical chemistry. Experience in the field of molecular spectroscopy and/or optical microscopy is required. She/he must have a strong attitude to work in an interdisciplinary team and will be available to travelling between Genoa and Milan according to experimental needs. Main working laboratory is at the Department of Nanophysics at the Central Research Laboratories of IIT, Genoa. Duration of the project is 2 years. To apply please submit your CV with a list of publications and two reference letters toalberto.diaspro(a)iit.it, annamaria.petrozza(a)iit.it and benjamin.harke(a)iit.it (cc.manuela.salvatori(a)iit.it) Candidatures received by the 31st of December will be considered. For any further information, please feel free to contact us using the email addresses above. In order to comply with the Italian law (art. 23 of Privacy Law of the Italian Legislative Decree n. 196/03), we have to kindly ask the candidate to give his/her consent to allow IIT to process his/her personal data. We inform you that the information you provide will be used solely for the purpose of assessing your professional profile to meet the requirements of Istituto Italiano di Tecnologia. Your data will be processed by Istituto Italiano di Tecnologia, with headquarters in Genoa, Via Morego 30, acting as the Data Holder, using computer and paper based means, observing the rules on protection of personal data, including those relating to the security of data. Please also note that, pursuant to art.7 of Legislative Decree 196/2003, you may exercise your rights at any time as a party concerned by contacting the Data Manager. The Italian Institute of Technology is an Equal Opportunity Employer that actively seeks diversity in the workforce. Last Updated on Friday, 26 October 2012 11:24 thank you AD

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[FILM-Users 00268] FILM Club 26 Oct 2012, 1pm: Mirella Koleva - 'Super-resolution imaging using stochastic optical reconstruction microscopy (STORM)'
by Martin Spitaler 26 Oct '12

26 Oct '12

Dear microscopists, it's already approaching the end of the month again, so this week it's time for our FILM Club again: *FILM Club**October 2012: **Mirella Koleva - 'Super-resolution imaging using stochastic optical reconstruction microscopy (STORM)'* Introduction to the STORM super-resolution imaging modality - basic principles, optimisation and areas for future improvement. * Friday 26 Oct 2012, 13.00-14.00, South Kensington / SAFB / MDL1.** * => Mind the changed location this week! When you walk into the Fleming Building, walk PAST the stairs (not up), then turn right to the lifts and go up to the first floor. Up there, just turn left and you are there. We've had a few presentations about the basic principles of super-resolutions microscopy and PALM in previous FILM Clubs and Microscopy Days, but Mirella will go a step further and go further in depth with regard to issues specific for STORM, but also very interesting for all other kinds of microscopy, like photo-physical properties of fluorescent dyes, dark states and photobleaching, calculating the resolution, visualising the localisation precision and more. See you there, good imaging, Martin -- ------------------------------------------------------------------------ *Martin Spitaler, PhD* *FILM - Facility for Imaging by Light Microscopy* - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk <mailto:m.spitaler@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

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[FILM-Users 00273] Fwd: Fwd: Digital Image Processing Workshop 2012
by Martin Spitaler 25 Oct '12

25 Oct '12

...forwarded, for your information, Martin Begin forwarded message: > From: Sarah Bebb <sarah(a)rms.org.uk> > Date: 25 October 2012 15:44:42 BST > To: Undisclosed recipients:; > Subject: Digital Image Processing Workshop 2012 > > take a closer look... > Digital Image Processing Workshop > > > > Modern light microscopy is highly quantitative and involves collection, storage and analysis of digital images. The recent revolution in domestic digital imaging means that many people are now familiar with digital imaging and some will have experience of basic image processing and image manipulation. > > > > > Further information > > > This one day RMS workshop meeting covers the underlying principles of digital image processing and enables the advanced user of light microscopy to make the very best use of their imaging data. The types of image vary from those of high spatial resolution obtained using confocal microscopy to lower resolution video and multidimensional data obtained using advanced optical techniques (like FRET, FRAP, multiphoton). > > This workshop course consists of two lectures that introduce the basic concepts of digital image processing and image analysis. The main focus of the day is for delegates to participate in a hands-on, computer-based workshop in which digital image analysis will be learnt by example. Workshop materials will be provided in advance of the meeting in the form of a CDROM and worksheets. > > The small group, workshop format, will enable people to progress at their own pace and work through the basics of signal processing and data analysis using Excel spreadsheets and progressing to image processing and image analysis using Image. The course will be supported by expert demonstrators. > > The workshop aims to advance the understanding and use of digital image processing and image analysis tools and initiate a network of interactions between young UK researchers that will be mediated and supported by the Light Microscopy Section of the RMS. > > > > http://www.rms.org.uk/events/Forthcoming_Events/dipw12 > > > > > Contact us >

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