- FILM-Users - imperial.ac.uk

[FILM-Users 00294] Fwd: RE: tet-regulated GFP
by Martin Spitaler 04 Dec '12

04 Dec '12

...forwarded, a request by a former FILM user; if you can help, please get in contact with Miranda directly ("Wilson, Miranda" <miranda.wilson(a)ucl.ac.uk>), Martin On 03/12/2012 14:06, Wilson, Miranda wrote: > Dear Martin > > I did an MRes project a few years ago with Alistair in the Seabra lab, > tracking melanosomes. You were really helpful back then and I wonder > if you could help me out again? These days I'm a postdoc at the MRC > institute at UCL. I'm hoping to get hold of a*tet-regulated GFP or GFP > fusion plasmid that I can use to help me screen the HCT116-tetR cell > line* I'm making. Do you know if anyone at SAF has one of those? > > Thanks and best wishes > Miranda > > > > Miranda S C Wilson, PhD > Postdoctoral Researcher > Saiardi Lab > MRC Laboratory for Molecular Cell Biology > University College London > Gower Street, London, WC1E 6BT > +44 207 679 2657 <tel:207%20679%202657> >

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[FILM-Users 00292] TODAY: FILM Club with Sunil Kumar - "Oblique Plane Microscopy for high-speed 2D and 3D imaging"
by Spitaler, Martin 30 Nov '12

30 Nov '12

TODAY: ================================== FILM CLUB November 2012: SUNIL KUMAR (Paul French lab) - "OBLIQUE PLANE MICROSCOPY FOR HIGH-SPEED 2D AND 3D IMAGING" Friday 30 Nov 2012, 1pm, SAFB-120 (South Kensington campus) Light Sheet Based Microscopy (LSBM) is a technique that allows the imaging of 3D specimens with improved speed and reduced photobleaching in comparison to techniques such as confocal microscopy. However, one drawback of conventional LSBM is that it requires the use of two separate microscope objectives for the illumination and imaging of the sample, and hence cannot be used with conventional sample preparation methods such as coverslips or multiwell plates. Oblique Plane Microscopy (OPM) is a technique developed at Imperial College that enables the use of light sheet techniques on conventionally-prepared samples through a single high NA microscope objective, allowing 2D imaging at >500 frames per second and volumetric imaging at up to 30 volumes per second, on samples ranging from single cells to individual organs in live zebrafish. See you all there, good imaging, Martin -- ________________________________ Martin Spitaler, PhD FILM - Facility for Imaging by Light Microscopy - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk<mailto:m.spitaler@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

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[FILM-Users 00291] FILM Club this Friday - changed topic: Sunil Kumar - "Oblique Plane Microscopy for high-speed 2D and 3D imaging"
by Martin Spitaler 28 Nov '12

28 Nov '12

Dear microscopists, unfortunately Zoltan had to cancel his FILM Club, but luckily we have found an excellent replacement with Sunil's presentation (thanks Sunil for agreeing at such short notice!!): FILM CLUB November 2012: SUNIL KUMAR (Paul French lab) - "OBLIQUE PLANE MICROSCOPY FOR HIGH-SPEED 2D AND 3D IMAGING" Friday 30 Nov 2012, 1pm, SAFB-120 (South Kensington campus) Light Sheet Based Microscopy (LSBM) is a technique that allows the imaging of 3D specimens with improved speed and reduced photobleaching in comparison to techniques such as confocal microscopy. However, one drawback of conventional LSBM is that it requires the use of two separate microscope objectives for the illumination and imaging of the sample, and hence cannot be used with conventional sample preparation methods such as coverslips or multiwell plates. Oblique Plane Microscopy (OPM) is a technique developed at Imperial College that enables the use of light sheet techniques on conventionally-prepared samples through a single high NA microscope objective, allowing 2D imaging at >500 frames per second and volumetric imaging at up to 30 volumes per second, on samples ranging from single cells to individual organs in live zebrafish. See you all there, good imaging, Martin -- ------------------------------------------------------------------------ *Martin Spitaler, PhD* *FILM - Facility for Imaging by Light Microscopy* - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk <mailto:m.spitaler@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

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[FILM-Users 00290] FILM Club Friday 30 Nov 1pm: Zoltan Kis - "Synthetic gene network for monitoring shear stress sensor activity in endothelial cells"
by Martin Spitaler 26 Nov '12

26 Nov '12

Dear microscopists, it's end of November again, which means the last FILM Club of the year is coming up, and we are finishing the year in good style with a very interesting one: Zoltan Kis (Rob Krams' lab, Bioengineering): "Synthetic gene network for monitoring shear stress sensor activity in endothelial cells" Friday 30 November 2012, 1pm, SAFB-120 (Fleming Building, South Kensington Campus) >>> http://www3.imperial.ac.uk/imagingfacility/news#filmclub <<< The background:The project is about combining mouse and mathematical models to study and identify new signalling pathways in endothelial cells covering an atherosclerotic plaque. The identified networks are synthesized in primary cells to study their reaction to flow. The high resolution 3-D lumen-vessel wall reconstruction, derived from imaging on FILM microscopes, is used for computational fluid dynamics. Features of the resulting stress/strain and/or protein distributions are used to identify regions of interest on digitally-derived cross sections. In line with our FILM Club series, another exciting talk showing how to get much more out of our microscopes than just images. Looking forward to seeing you there, good imaging, Martin -- ------------------------------------------------------------------------ *Martin Spitaler, PhD* *FILM - Facility for Imaging by Light Microscopy* - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk <mailto:m.spitaler@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

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[FILM-Users 00289] Re: FILM Image Of The Year 2012 competition
by Martin Spitaler 23 Nov '12

23 Nov '12

Just a reminder about our competition, don't forget to submit your images, Martin > > *From:*film-users-bounces@imperial.ac.uk > [mailto:film-users-bounces@imperial.ac.uk] *On Behalf Of *Martin Spitaler > *Sent:* 16 November 2012 16:57 > *To:* film-users > *Subject:* [FILM-Users 00283] FILM Image Of The Year 2012 competition > > Dear microscopists, > > it is back, bigger and better than ever - the FILM IMAGE OF THE YEAR > 2012 competition! Again, great prizes are waiting to be won, and this > time everybody is a winner: > > Winner - an iPod Nano! ...for those long hours at the microscope > Runners-up: a beautiful A3 poster calendar, worth £18! > ALL PARTICIPANTS: 30% discount on Molecular Probes antibodies! > > Deadline for submission of your images: Wednesday 5th of December 2012 > (see also the website: > http://www3.imperial.ac.uk/imagingfacility/research/gallery/film_image_of_t…) > > Judges > As in previous years, we have a prestigious panel of independent > judges to select your best images: > > Kathy Nobel, Tate Modern > Alex Ball, Natural History Museum > Tatiana Schofield, Imperial College Consultants > Melissa Belli, artist > > > Sponsorship > As before, we thank Molecular Probes / Life Technologies for their > generous sponsorship! > > > Rules for participation > Up to 3 images per participant, but they must show different samples > (not different versions of the same image) > Images must have been taken on FILM microscopes (original data files > must be made available) > Images will be judged purely on their visual impression, they do not > need to show any scientific results > The best possible visualisation of the image data is encouraged (e.g. > contrast enhancement, look-up tables), but manipulation of pixel data > is not allowed - the scientific content is still visible; example for > , manipulations that are not allowed are adding / removing of objects > (retouching) or collages (copying objects together) > By submitting the images, participants grant FILM the right to make > the entries publicly available on the FILM website, use them for the > calendars or other presentations; please make sure they don't contain > any identifiable unpublished information > > So don't miss out, go through your microscopy images and go for the > prizes! > > Good imaging, > > Martin > > -- > > ------------------------------------------------------------------------ > > *Martin Spitaler, PhD* > > *FILM **- Facility for Imaging by Light Microscopy* > - Facility Manager - > Sir Alexander Fleming Building, desk 401 > Imperial College London / South Kensington > Exhibition Road > London SW7 2AZ > UK > > Tel. +44-(0)20-759-42023 > E-mail m.spitaler(a)imperial.ac.uk <mailto:m.spitaler@imperial.ac.uk> > Website: http://imperial.ac.uk/imagingfacility >

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[FILM-Users 00288] Fwd: Imperial Flow Course 2013 --Save the dates 12th/13th September 2013
by Martin Spitaler 20 Nov '12

20 Nov '12

...forwarded for your information - you can't complain you didn't get enough notice, Martin -------- Original Message -------- Subject: Imperial Flow Course 2013 --Save the dates 12th/13th September 2013 Date: Sun, 18 Nov 2012 14:16:17 +0000 From: Poobalasingam, Thurka <t.poobalasingam(a)imperial.ac.uk> Dear Flow Cytometry Facility users, Please pencil these dates into your diraries for the next Imperial Flow Cytometry course: Thursday 12 and Friday 13 September 2013 The programme will be slightly different with more emphasis on the basics (based some of the feedback we receieved); * Basics to flow cytometry * Hydrodynamic focus in flow * staining * Tritration of antibodies * Compensation * Standardisation and CST * Cell viability staining options --differences for fixed and unfixed cells * panel design workshop * data analysis workshops using various analysis software will be available to enable you to display your data in the best possible way. * Apoptosis * Cell cycle * Microscopy and the Imagestream will also be covered to help our users combine the cross-over of flow and imaging. * the second day will still hold multi-colour flow talks to help you expand your current flow panel * new technologies related to flow TBC * Cell sorting * Multi-plexing A more detailed programme will be confirmed soon with our list of speakers, titles and abstracts. Kind regards *Thurka Poobalasingam* * * *Flow Cytometry Technician* *Respiratory Infections Section* *Respiratory Sciences * *National Heart & Lung Institute (NHLI)* *Imperial College London* *Room 312D, Third floor FACS Facility, * *Medical School Building* *St. Mary's Campus* *Norfolk Place* *Paddington* *London W2 1PG* *Tel: 020 7594 3763* *Email: t.poobalasingam(a)imperial.ac.uk* *Website: * *http://www1.imperial.ac.uk/medicine/about/institutes/centre_respiratory_infection/flow/* * * * * *

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[FILM-Users 00287] Fwd: Seminar TODAY: Regine Hengge, Freie Universität in Berlin: Cylic-di-GMP signalling and high resolution spatial order in macrocolony biofilms of E. coli
by Martin Spitaler 19 Nov '12

19 Nov '12

...forwarded for your information, Martin Cylic-di-GMP signalling and high resolution spatial order in macrocolony biofilms of E. coli Professor Regine Hengge, from Freie Universität in Berlin, leads this lunchtime seminar Date: 19 Nov 2012 Time: 12:30 - 13:30 Venue: G34, Sir Alexander Fleming Building Campus: South Kensington Campus Speaker: Professor Regine Hengge Contact: Kylie Glasgow Abstract Bacterial macrocolonies represent structured biofilms that can produce wrinkled, ring- or radiation-like patterns, whose formation depends on motility and an extracellular matrix of adhesins, amyloid fibres (e.g. curli) and polysaccharides (e.g. cellulose). While flagella are synthesized by postexponentially growing rod-shaped E. coli cells, curli fibres and cellulose are produced during entry into stationary phase, with starving cells becoming smaller and ovoid. This transition is under exquisite control of the general stress sigma factor RpoS and the ubiquitous bacterial second messenger cyclic-di-GMP (2). The latter is produced by diguanylate cyclases (DGC, with GGDEF domains) and is degraded by specific phosphodiesterases (PDE, with EAL or HD-GYP domains). c-di-GMP controls a variety of targets, including transcription and the activities of enzymes and complex cellular structures. Many bacterial species possess multiple GGDEF/EAL proteins (29 in E. coli K-12), which has led to the concept of temporal and functional sequestration of locally acting c-di-GMP control modules (1). The presentation will focus on a cascade of two distinct c-di-GMP control modules. C-di-GMP synthesized by the first of these modules antagonizes an inhibitor of the second one, which constitutes a locally acting signaling complex of a DGC, the inhibitor and a c-di-GMP-binding transcription factor. This protein complex controls the expression of the biofilm regulator CsgD, which in turn regulates the biosynthesis of curli fibres and cellulose. Using fluorescence and scanning EM, it was found that this cascade is active in the top layer of a macrocolony biofilm, where small starved cells are literally encased in a thick network of curli fibres. By contrast, the bottom of the biofilm is formed by still growing cells surrounded by a tight mesh of entangled flagella, the formation of which requires flagellar motor function. The inner regions of the biofilm show heterogeneous curli expression. Cells in the outer rim growth zone produce flagella, which rap around and tie cells together. Overall, this study reveals the microanatomy of a bacterial biofilm and the underlying regulatory networks in unprecedented detail. 1. Hengge, R. 2009. Principles of cyclic-di-GMP signaling. Nature Rev. Microbiol. 7:263-273. 2. Hengge, R. 2011. The general stress response in Gram-negative bacteria, p. 251-289. In G. Storz and R. Hengge (ed.), Bacterial Stress Responses. ASM Press, Washington, D.C.

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[FILM-Users 00286] Fwd: PLUG, SHOOT & WIN by Motic
by Martin Spitaler 16 Nov '12

16 Nov '12

...forwarded for your information... ...Now that you are on it, preparing your best images for the FILM Image Of The Year competition, why not re-use them and top up the iPod Nano with an iPad? Good luck! Martin -------- Original Message -------- Subject: PLUG, SHOOT & WIN by Motic Date: Fri, 16 Nov 2012 12:52:49 +0100 From: Marketing Department - Motic Europe <mailing.es(a)moticeurope.com> Reply-To: <contest(a)moticeurope.com> To: <m.spitaler(a)imperial.ac.uk> Motic Europe, November 2012 MoticNews Special Edition PLUG, SHOOT & WIN: Motic's first photomicrography contest To celebrate our Moticam's 10th year anniversary, we are proud to present the first edition of the "PLUG, SHOOT & WIN!" photomicrography contest. To find out all there is to know about it - including how you can win an iPad or iPod - click here www.moticeurope.com/contest and show us what you are capable of! Let's make this the first of many editions. For further information, please do not hesitate to contact us If you do not wish to receive the Motic Newsletter anymore, please send an email to: unsubscribe(a)moticeurope.com

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[FILM-Users 00285] Fwd: [neuroscience-technology-network] Fwd: [Cognition, Perceptual and Brain Sciences Seminar] Davide Zoccolan, 4pm, 27th Nov 2012
by Martin Spitaler 16 Nov '12

16 Nov '12

...forwarded for your information, Martin -------- Original Message -------- > From: Sam Solomon <samuels(a)physiol.usyd.edu.au> > Date: 13 November 2012 22:09 > To: Matteo Carandini <matteo(a)carandinilab.net>, Alan Johnston <a.johnston(a)ucl.ac.uk>, "Joshua A. Solomon" <J.A.Solomon(a)city.ac.uk>, "Steven Dakin" <s.dakin(a)ucl.ac.uk>, Simon Schultz <s.schultz(a)imperial.ac.uk>, m.hausser(a)ucl.ac.uk > Subject: [Cognition, Perceptual and Brain Sciences Seminar] Davide Zoccolan, 4pm, 27th Nov 2012 > Dear all, > I'm not exactly sure of how to write to the various vision-interest and lab lists that are run, but could we try to advertise the following talk. > Davide does very interesting stuff. > Best wishes > Sam > > *************************************************************************** > Cognition, Perceptual and Brain Sciences Seminar Series > *************************************************************************** > > Speaker: Davide Zoccolan, SISSA, Trieste > Title: A rodent model for the study of invariant visual object recognition > Time: 4pm, Tuesday 27th November, 2012 > Place: Room 305, 26 Bedford Way, London > Abstract: The ability to recognize objects despite tremendous variation in their appearance (e.g., because of position or size changes) represents such a formidable computational feat that it is widely assumed to be unique to primates. Such an assumption has restricted the investigation of its neuronal underpinnings to primate studies, which allow only a limited range of experimental approaches. In recent years, the increasingly powerful array of optical and molecular tools that has become available in rodents has spurred a renewed interest for rodent models of visual functions. However, evidence of primate-like visual object processing in rodents is still very limited and controversial. > In this seminar, I will present behavioral evidence showing that rats are capable of recognizing visual objects in spite of substantial variation in their appearance, i.e., in spite of changes in size, position, illumination, in-depth rotation and in-plane rotation. I will also show that such a transformation-tolerant (or invariant) recognition is largely accounted by rat ability to spontaneously perceive different views/appearances of an object as similar (i.e., as instances of the same object). Finally, I will show that rat object recognition relies on a shape-based, multi-featural processing strategy that makes close-to-optimal use of the discriminatory information afforded by the target objects across their various appearances. > Overall, these findings indicate that rats are able to process and efficiently use shape information, in a way that is largely tolerant to variation in object appearance, thus supporting spontaneous generalization of recognition to previously unseen views of learned objects. This suggests that their visual system may serve as a powerful model to study the neuronal substrates of invariant visual object recognition.

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[FILM-Users 00284] Fwd: Webinar: Quantification of cellular dynamics - in vivo and ex vivo microscopy (3/4D) investigations (commercial posting)
by Martin Spitaler 16 Nov '12

16 Nov '12

...forwarded for your information, Martin *Subject:* Webinar: Quantification of cellular dynamics - in vivo and ex vivo microscopy (3/4D) investigations (commercial posting) *From:* Daniel McRitchie <d.mcritchie(a)ANDOR.COM> *Reply-To:* Confocal Microscopy List <CONFOCALMICROSCOPY(a)LISTS.UMN.EDU> *Date:* Tue, 13 Nov 2012 10:33:15 -0600 *Content-Type:* text/plain ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Title: Quantification of cellular dynamics - in vivo and ex vivo microscopy (3/4D) investigations Date: 15th November 2012 Time: 4pm GMT (11am EST) Duration: 60 minutes Find out more -http://goo.gl/pAXcX Speakers: Dr. Staffend, Michigan State University Dr. Celli, Pasteur Institute Dr. Matthew Gastinger, Support Manager, Bitplane Moderator: Simone Groothuis, Cellular Signalling Journal Who should attend: This webinar is particularly useful for Neuroscientists, Immunologists, Cell Biologists, as well as researchers from other fields who also use 3D/4D imaging methods such as confocal, multiphoton, or equivalent. To register click here -http://goo.gl/pAXcX

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