- FILM-Users - imperial.ac.uk

[FILM-Users 00437] files deleted on temporary server
by Martin Spitaler 26 Nov '13

26 Nov '13

Dear microscopists, this is a reminder that the FILM server <\\film-nas.med.ic.ac.uk\SCRATCH> is only to be used as a temporary storage space to move files around, NOT to store any data. Files on the server can and will be removed whenever space is needed WITHOUT NOTICE. This is to make sure all users can smoothly use the microscopes without worrying about copying data around. If you need space to STORE your data, we highly recommend to use the double-backed up space provided by the Bioinformatics Support Service, including the very useful OMERO Image Database. That's what we use for our image data in FILM, because it's the best solution we have found. For more information see FILM website / GUIDELINES / DATA / DATA MANAGEMENT for more information. <http://www3.imperial.ac.uk/imagingfacility/facility_guidelines/data_managem…> Right now, the server is again nearing capacity, so we will delete files to make space for new data over the next few days. If there is anything you need, please move it elsewhere quickly. Good imaging, Martin -- ------------------------------------------------------------------------ *Martin Spitaler, PhD* *FILM - Facility for Imaging by Light Microscopy* - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk <mailto:m.spitaler@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

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[FILM-Users 00436] eYFP construct needed
by Martin Spitaler 22 Nov '13

22 Nov '13

Forwarded on behalf of Morgan Beeby - can anybody help? Best regards, Martin -------- Original Message -------- Subject: FW: fluorescent protein pointers? Date: Wed, 20 Nov 2013 12:44:07 +0000 From: Beeby, Morgan <m.beeby(a)imperial.ac.uk> To: Spitaler, Martin <m.spitaler(a)imperial.ac.uk> Dear Martin, Apologies for the harassment, but I wondered if you had any thoughts on possible people to talk to regarding obtaining eYFP (or other fluorescent protein)-encoding vectors? thanks! Morgan

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[FILM-Users 00425] FILM Image Of The Year 2013-14
by Martin Spitaler 22 Nov '13

22 Nov '13

Dear microscopists, we are slowly nearing the end of the year again, so it's time for our annual competition again, the FILM IMAGE OF THE YEAR 2013-14! Again, thanks to generous sponsorship from Molecular Probes / Life Technologies, attractive prizes are waiting: *Winner *- an*iPod Nano*! ...for those long hours at the microscope _Runners-up_: a beautiful _A3 poster calendar_, worth £23! *Deadline* for submission of your images: *Friday 29th of December 2012* _*Judges *_ As in previous years, we have a prestigious panel of independent judges to select your best images: Kathy Nobel, Tate Modern Alex Ball, Natural History Museum Tatiana Schofield, Imperial College Consultants Melissa Belli, artist _ _ _Rules for participation_ * Up to 3 images per participant; they must show different samples (not different versions of the same image) * Images must have been taken on FILM microscopes (original data files must be made available) * Images will be judged purely on their visual impression, they do not need to show any scientific results, but a description of the scientific context is encouraged (and might make it more interesting for the judges) * The best possible visualisation of the image data is encouraged (e.g. contrast enhancement, look-up tables), but manipulation of pixel data is not allowed - the scientific content must still visible. Examples of manipulations not allowed are adding / removing of objects (retouching) or collages (copying objects together) * By submitting the images, participants grant FILM the right to make the entries publicly available on the FILM website, use them for the calendars or other presentations describing research peformed in the facility; please make sure they don't contain any identifiable unpublished information For inspirations from previous years and details see the website: <http://www3.imperial.ac.uk/imagingfacility/research/gallery/film_image_of_t…> So don't miss out, get your best microscopy images and go for the prizes! Good imaging, Martin -- ------------------------------------------------------------------------ *Martin Spitaler, PhD* *FILM - Facility for Imaging by Light Microscopy* - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk <mailto:m.spitaler@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

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[FILM-Users 00433] Fwd: Molecular Medicine & Cell Biology Seminar Series | Tue 12 Nov 2013 | 12.00 - 1.00pm room 120 SAF
by Martin Spitaler 11 Nov '13

11 Nov '13

...forwarded for your information Martin Flyer: <https://workspace.imperial.ac.uk/imagingfacility/Public/seminar-131112b.pdf> -------- Original Message -------- Subject: Molecular Medicine & Cell Biology Seminar Series | Tue 12 Nov 2013 | 12.00 - 1.00pm room 120 SAF Date: Mon, 11 Nov 2013 10:55:08 +0000 From: Enright, Catherine E <c.enright(a)imperial.ac.uk> Molecular Medicine & Cell Biology Seminar Series Molecular Medicine | National Heart and Lung Institute & Cell Biology | Division of Medicine Faculty of Medicine | Imperial College London *Dr Elisabeth Ehler** *The Randall Centre of Cell and Molecular Biophysics, King's College London. *'Formin-g and maintaining myofibrils in the heart'** * Host Dr Pradeep Luther *Tuesday 12 November 2013 | 12:00-1:00pm** **Room 120 | Sir Alexander Fleming Building | South Kensington Campus** *ALL WELCOME

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[FILM-Users 00423] Phase Focus demo
by Martin Spitaler 11 Nov '13

11 Nov '13

Dear microscopists, see below the invitation to a demonstration of a new microscope technology, Ptychography, by Joanne Whetstone from the company Phasefocus. It's label free and three-dimensional, so potentially interesting to a range of users. Good imaging, Martin -------- Original Message -------- Subject: Re: Phase Focus demo Date: Mon, 28 Oct 2013 14:44:54 +0000 From: Joanne Whetstone <joanne.whetstone(a)phasefocus.com> *==> PDF version of this invitation*: <https://workspace.imperial.ac.uk/imagingfacility/Public/seminar.pdf> Phasefocus would like to invite you to a presentation on Monday 11th November at 12pm, and a demonstration of their high contrast label-free live cell imaging system on Tuesday 12th or Wednesday 13th November 2013. The Phasefocus Virtual Lens® uses a technique known as Ptychography, allowing phase imaging of cells in high contrast, without using labels, and free of artefacts. This method adds a further quantitative dimension to phase imaging, and is able to provide numerical information on cell cycle analysis, cell thickness, and cell volume, which has traditionally been measured using fluorescence. The system also uses low laser power allowing long term timelapse experiments to be performed. PRESENTATION Monday 11th November 12:00 Room SAFB-121, South Kensington Campus, Exhibition Road, London SW7 2AZ Refreshments will be provided DEMONSTRATION Room SAFB-530, South Kensington Campus, Exhibition Road, London SW7 2AZ We want you all to have the opportunity to see the system and ask questions, the easiest way to do this is to allocate timeslots for you to sign up for. These are: Tues12th and Wed 13th November: 09:30, 11:00, 13:00, 14:30, 16:00 To make a booking, please contact joanne.whetstone(a)phasefocus.com, 07748 963287. We are flexible, so if these times don't suit you, please contact us and we will do our best to accommodate you. Joanne Whetstone Sales & Marketing Manager -- Phase Focus Logo <http://www.phasefocus.com/> *Joanne Whetstone *Sales & Marketing Manager PHONE: +44 (0)114 286 6364 MOBILE:_+44 (0) 7748 963287_ WEB: www.phasefocus.com <http://www.phasefocus.com/> *Phasefocus Limited* Electric Works Sheffield Digital Campus Sheffield, UK S1 2BJ Facebook <https://www.facebook.com/pages/Phase-Focus/434726259956299?fref=ts>Twitter <https://twitter.com/PhaseFocus1>LinkedIn <http://www.linkedin.com/company/1290490>Phasefocus Blog <http://phasefocus.blogspot.co.uk/>Phasefocus Youtube Channel <http://www.youtube.com/channel/UCuiyDWj5fmp6VfXiflGSGyA> This email is confidential and may also be privileged. Unless you are the intended recipient, you may not copy, forward or otherwise use it, or disclose it or its contents to anyone else. If you have received this email in error please notify us immediately and delete it from your system. ------------------------------------------------------------------------ Company registered in England No. 05856137. Reg'd Office: 40 Leavy Greave Road, Sheffield, South Yorkshire, S3 7RD

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[FILM-Users 00431] anti-Mouse IgG-Alexa 647
by Xu, Huifang 08 Nov '13

08 Nov '13

Dear All, I am in need of a couple of ul of the anti-Mouse IgG-Alexa647 as secondary antibody for my IF staining. Wondering if anyone happen to have some that I could borrow? Thank you very much! Best wishes, Huifang

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[FILM-Users 00429] correction: seminar information
by Martin Spitaler 06 Nov '13

06 Nov '13

Dear microscopists, there has been a mix-up of the links to the PDF flyers for invitations to upcoming seminars, here is the correct information: ------------------------------------------------------------------------ Monday 11 November, 12.00, SAFB-121: *PhaseFocus imaging *flyer: <https://workspace.imperial.ac.uk/imagingfacility/Public/seminar-131112.pdf>* * ------------------------------------------------------------------------ Tuesday 13 November, 14.00, Blackett Lecture Theatre 3: *Safe use of lasers in research* flyer: <https://workspace.imperial.ac.uk/imagingfacility/Public/seminar-131113.pdf>* * ------------------------------------------------------------------------ Wednesday 11 December, 17.00, Blackett Lecture Theatre 3*: Leica Scientific Forum, Eric Betzig: Imaging Life at High Spatiotemporal Resolution *flyer: <https://workspace.imperial.ac.uk/imagingfacility/Public/seminar-131211.pdf>* * ------------------------------------------------------------------------ Sorry for the confusion, good imaging, Martin * * -- ------------------------------------------------------------------------ *Martin Spitaler, PhD* *FILM - Facility for Imaging by Light Microscopy* - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk <mailto:m.spitaler@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

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[FILM-Users 00430] Fwd: 2 weeks left to register for Live Cell Imaging - Opportunities and Challenges - Wednesday 20 Nov 13 - Cambridge
by Martin Spitaler 06 Nov '13

06 Nov '13

...forwarded for your information - and don't forget that many more events can be found on the FILM website under LINKS & EVENTS / MEETINGS, COURSES, COMPETITIONS, Martin -------- Original Message -------- Subject: 2 weeks left to register for Live Cell Imaging - Opportunities and Challenges - Wednesday 20 Nov 13 - Cambridge Date: Wed, 6 Nov 2013 08:47:39 -0500 From: Beverley Vaughan <info(a)univercellmarket.com> To: <m.spitaler(a)IMPERIAL.AC.UK> Register now for Opportunities and Challenges in Live Cell Imaging <http://click.icptrack.com/icp/relay.php?r=51457770&msgid=271976&act=RPJU&c=…> Good afternoon We hope you will be able to join our panel of imaging experts in Cambridge on Wednesday 20 November2013 and share in a lively discussion on the "Opportunities and Challenges in Live Cell Imaging". <http://click.icptrack.com/icp/relay.php?r=51457770&msgid=271976&act=RPJU&c=…> This meeting will address the current challenges associated with *live cell imaging* and ask how improvements can be made in key areas. We have presentations from Dr Peter O'Toole, York University;Dr Beverley Isherwood, AstraZeneca; Professor John Girkin, Durham University; Professor Paul French, Imperial College London and Dr Loic Lhuillier, Technology Strategy Board, to name just a few. The full programme can be found on our website. <http://click.icptrack.com/icp/relay.php?r=51457770&msgid=271976&act=RPJU&c=…> By attending this informal and highly practical conference, in the course of just one day you will: · gain an understanding of the next generation imaging systems including imaging technologies from disciplines such as astronomy and photonics · learn from the experience of other users · identify opportunities for collaborating with other members of the imaging community We have priced the tickets competitively at £150 plus VAT for commerical delegates and £50 + VAT for academic delegates to attract a wide range of participants. For more information and to book your place, please visit our website. <http://click.icptrack.com/icp/relay.php?r=51457770&msgid=271976&act=RPJU&c=…> "Opportunities and Challenges in Live Cell Imaging” <http://click.icptrack.com/icp/relay.php?r=51457770&msgid=271976&act=RPJU&c=…> is being hosted with the support of the Electronics, Photonics and Sensors Knowledge Transfer Network (ESP KTN). We look forward to welcoming you to the Hauser Forum in Cambridge on the 20th November. Best wishes, Dr Beverley Vaughan Director of Operations univerCELLmarket Ltd., St John's Innovation Centre Cowley Road Cambridge, CB4 0WS www.univercellmarket.com <http://click.icptrack.com/icp/relay.php?r=51457770&msgid=271976&act=RPJU&c=…> beverley(a)univercellmarket.com <mailto:beverley@univercellmarket.com?subject=Enquiry%20from%20Live%20Cell%20Imaging%20Invitation> univerCELLmarket - global resource for stem cells and regenerative medicine <http://click.icptrack.com/icp/relay.php?r=51457770&msgid=271976&act=RPJU&c=…> This message was sent to m.spitaler(a)IMPERIAL.AC.UK from: Cathy Prescott | St John's Innovation Centre, Cowley Road | Cambridge, Cambs CB4 0WS, United Kingdom Email Marketing by iContact - Try It Free! <http://www.icontact.com/a.pl/144186> Manage Your Subscription <http://app.icontact.com/icp/mmail-mprofile.pl?r=51457770&l=2314&s=RPJU&m=27…>

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[FILM-Users 00428] Fwd: FW: FoNS safety seminar - 'Safe use of lasers in research' 13th November
by Martin Spitaler 06 Nov '13

06 Nov '13

...forwarded for your information; recommended for anyone using two-photon microscopy, laser tweezers, TIRF, PALM, STORM, ... Martin -------- Original Message -------- Subject: FW: FoNS safety seminar - 'Safe use of lasers in research' 13th November Date: Wed, 6 Nov 2013 09:43:20 +0000 From: Koch, Helga <helga.koch(a)imperial.ac.uk> To: Magee, Tony <t.magee(a)imperial.ac.uk>, Spitaler, Martin <m.spitaler(a)imperial.ac.uk> Good Morning, Please see below invitation to attend a laser safety seminar and feel free to forward this invitation to relevant laser users in you Section. Regards Helga *From:*Hoyle, Stefan L B *Sent:* 06 November 2013 08:49 *To:* Gillett, Ian; de Paiva, Anton M; Cotton, Julia N; Morgan, Ross; Fear, Jon C; Robertson, Brian; Luke, John; Greenwood, Stephen R; Koch, Helga *Cc:* Hirjee, Nazia; Rose, Steven J; de Abela-Borg, Jan; Easton, Julia M *Subject:* FoNS safety seminar - 'Safe use of lasers in research' 13th November Dear all, As discussed with Ian previously we have arranged for two academics to give talks on safe use of lasers in research to help with our current plan on raising awareness of health and safety around lasers and the application of engineering controls in the Faculty. Attached is the poster associated with the event. If you can attend that will be great. Steve / Helga if you want to advertise this to relevant laser users in your Faculties please feel free to do so. We do ask that anyone attending register so we can advise catering on numbers for the lunch beforehand. To register follow the link: _http://www3.imperial.ac.uk/physics/safety/laserworkshop _ (or on the poster). Many thanks Stef Poster: <https://workspace.imperial.ac.uk/imagingfacility/Public/seminar2.pdf> Stefan Hoyle MSc CMIOSH Imperial College London Faculty of Natural Sciences Head of Health and Safety 5th Floor Sir Alexander Fleming Building South Kensington Campus, London SW7 2AZ Tel: 07872850018 / Mobex: 50018 s.hoyle(a)imperial.ac.uk <mailto:s.hoyle@imperial.ac.uk> http://www3.imperial.ac.uk/naturalsciences

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[FILM-Users 00427] Fwd: endothelial cell lines
by Martin Spitaler 05 Nov '13

05 Nov '13

Forwarded on behalf of Zoltan Kis - can anybody help? Martin -------- Original Message -------- Subject: endothelial cell lines Date: Tue, 5 Nov 2013 11:47:15 +0000 From: Kis, Zoltan <z.kis10(a)imperial.ac.uk> To: Spitaler, Martin <m.spitaler(a)imperial.ac.uk> Dear Martin, Could you please send an email to FILM users asking whether someone could give us any of the following endothelial cell lines? MS1 (MILE SVEN 1) MS1 VEGF EVLC2 HUV-ST Any other endothelial cell line that expresses the SV40 large T antigen? (except the HMEC-1 cell line, which we already possess) Many thanks, Zoltan *************************************** Zoltán Kis, Bioengineering Ph.D. student Department of Bioengineering Imperial College London Royal School of Mines Building, office RSM4.28 Exhibition Road, London, SW7 2AZ, UK tel: +44 (0) 7907 491567 e-mail: z.kis10(a)imperial.ac.uk ***************************************

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