- FILM-Users - imperial.ac.uk

[FILM-Users 00402] new RGB camera and computer on WF2
by Martin Spitaler 13 Aug '13

13 Aug '13

Dear microscopists, with support from the College's Equipment Sharing Grant we now finally have an RGB camera for histology imaging on Widefield 2 (South Kensington). It has a nixe, large sensor with 2452 x 2056 pixels, 3x12bit digitisation. Thanks to this grant, we also have upgraded to a new 64-bit Win-7 computer, so we can now run the newest version of the Hamamatsu HCImage software, which allows automated tiling and stitching of large areas. In combination with the new camera, this will finally allow efficient acquisition of large histology slides. If this is what you need, please get in touch. For those located in Hammersmith: HWF1 has had this functionality already. Good imaging, Martin -- ------------------------------------------------------------------------ *Martin Spitaler, PhD* *FILM - Facility for Imaging by Light Microscopy* - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk <mailto:m.spitaler@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

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[FILM-Users 00401] Fwd: H2BTagRFP
by Martin Spitaler 12 Aug '13

12 Aug '13

...can anybody help Nicola? Best regards, Martin -------- Original Message -------- Subject: H2BTagRFP Date: Mon, 12 Aug 2013 12:36:54 +0100 From: Ruivo, Nicola R <n.ruivo(a)imperial.ac.uk> To: Spitaler, Martin <m.spitaler(a)imperial.ac.uk> Hi Martin Do you know of any group that might have or is using H2BTagRFP for imagining? We're in search a donation of some DNA if anyone would be willing to help us out. Kind regards, Nicola *Nicola Ruivo (M.Sc.)* *Lo Celso Laboratory* *Senior Research Technician * *Imperial College London* *Cell & Molecular Biology* *Sir Alexander Fleming Building* *South Kensington Campus* *SW7 2AZ* *email: n.ruivo(a)imperial.ac.uk <mailto:n.ruivo@imperial.ac.uk>* *Telephone: 020 7594 2466 * 108102810593603702

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[FILM-Users 00400] Fwd: Super resolution microscopy post doc post.
by Martin Spitaler 09 Aug '13

09 Aug '13

...forwarded for your information, Martin -------- Original Message -------- Subject: Super resolution microscopy post doc post. Date: Fri, 9 Aug 2013 15:24:07 +0100 From: Nick Barry <nbarry(a)MRC-LMB.CAM.AC.UK> Reply-To: Nick Barry <nbarry(a)MRC-LMB.CAM.AC.UK> To: <UK-EUROBIOIMAGING-PROJECT(a)JISCMAIL.AC.UK> Dear All I am currently advertising for a post doc to work on super resolution imaging . The sucessful candidate would be awarded an MRC Career Development Fellowship. If you know of a suitable candidate please pass on the link http://www.nature.com/naturejobs/science/jobs/334781-postdoctoral-scientist Many thanks Nick Barry MRC Laboratory of Molecular Biology -- UK-EUROBIOIMAGING Visit the website http://www.bioimaginguk.org To e-mail the group, send messages to UK-EUROBIOIMAGING-PROJECT(a)JISCMAIL.AC.UK To subscribe visit https://www.jiscmail.ac.uk/cgi-bin/webadmin?A0=UK-EUROBIOIMAGING-PROJECT --

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[FILM-Users 00399] Fwd: Fw: AstraZeneca Recruitment
by Martin Spitaler 05 Aug '13

05 Aug '13

...forwarded for your information, could be an interesting job, especially if you have done a bit of high-throughput and image analysis - (AstraZeneca are intensive users of Definiens), Martin -------- Original Message -------- Subject: Fw: AstraZeneca Recruitment Date: Mon, 5 Aug 2013 10:35:42 +0100 From: Joanne Marrison <joanne.marrison(a)YORK.AC.UK> Reply-To: Joanne Marrison <joanne.marrison(a)YORK.AC.UK> To: <UK-EUROBIOIMAGING-PROJECT(a)JISCMAIL.AC.UK> Hi All, Please see below for recruitment opportunities at AstraZeneca, closing date 16th August Best wishes Jo -- Joanne Marrison Imaging and Cytometry Laboratory (B/K051) Bioscience Technology Facility, Department of Biology University of York, York YO10 5DD Tel: +44(0) 1904 328720 www.york.ac.uk/biology/tf <http://www.york.ac.uk/biology/tf> EMAIL DISCLAIMER http://www.york.ac.uk/docs/disclaimer/email. <http://www.york.ac.uk/docs/disclaimer/email.htm> We are recruiting senior scientists into our High Content Biology group at AstraZeneca. If you know of anyone who might be interested please feel free to share the advert. http://gs.globalsuccessor.com/fe/tpl_astrazenecaV2.asp?s=4A515F4E5A565B1A&j… Best wishes Bev *Bev Isherwood* Team Leader ___________________________________________ *AstraZeneca* R&D | Innovative Medicines | Discovery Sciences 14F3 Mereside, Alderley Park, Macclesfield, Cheshire, SK10 4TG Tel: +44 (0)1625 513109 <tel:%2B44%20%280%291625%20513109> (Extension 23109) _beverley.isherwood(a)astrazeneca.com <mailto:beverley.isherwood@astrazeneca.com>_ __ htm <http://www.york.ac.uk/docs/disclaimer/email.htm> -- UK-EUROBIOIMAGING Visit the website http://www.bioimaginguk.org To e-mail the group, send messages to UK-EUROBIOIMAGING-PROJECT(a)JISCMAIL.AC.UK To subscribe visit https://www.jiscmail.ac.uk/cgi-bin/webadmin?A0=UK-EUROBIOIMAGING-PROJECT --

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[FILM-Users 00398] Fwd: FW: EUBIAS2013: European BioImage Analysis Symposium in Barcelona, Oct. 7- 8th
by Martin Spitaler 02 Aug '13

02 Aug '13

...forwarded for your information, Martin -------- Original Message -------- Subject: FW: EUBIAS2013: European BioImage Analysis Symposium in Barcelona, Oct. 7- 8th Date: Fri, 2 Aug 2013 13:03:48 +0200 From: Stefan Terjung <terjung(a)embl.de> Reply-To: <terjung(a)embl.de> Organisation: EMBL To: <elmi(a)embl.de> *From:*Eubias2013 Organization [mailto:eubias2013@gmail.com] *Sent:* Freitag, 2. August 2013 03:34 *To:* stefan.terjung(a)embl.de; elmi(a)embl.de *Subject:* EUBIAS2013: European BioImage Analysis Symposium in Barcelona, Oct. 7- 8th Dear ELMI members, We will organize a quite unique type of bioimage analysis meeting in Barcelona, from 7th to 12th October this year. The meeting aims at strengthening the image analysis community in Europe and at increasing the accessibility of image analysis tools for the community and end-users. The event features ·An Open Community Meeting, (Oct. 7-8th, registration opened now), with "Software Showcase" presentations contributed by developers, open discussions and debates, and oral and posters contributions, participation open to everyone. ·A "Taggathon" (on invitation, Oct. 9-11th) to create an interactive webtool for the community, and to be discussed altogether previously at the meeting. ·A BioImage Analysis Course (BIAS 2, Oct. 9-12th) for imaging facility staffs, which includes participation to the Meeting. We welcome you to sign up to the BioImage Community Meeting on October 7th and 8th. Please visit the website below for more details and for registration: http://eubias2013.irbbarcelona.org We invite members of European Imaging facilities to apply now to the BIAS 2 course on Image Analysis and scientific programming (same link). We also ask you to please forward this email to your national imaging communities and co-workers. This event was conceptualized at the last ELMI meeting 2013 in Arcachon, is mainly sponsored by*EuroBioImaging *(www.eurobioimaging.org <http://www.eurobioimaging.org>) and *OME*, and by companies bridging open software (e.g. Bitplane). EuBIAS2013 is open to further sponsor contributions and participation from the private sector. EuBIAS2013 is hosted by IRB Barcelona and is organized by many people from the University of Dundee, EMBL (Heidelberg), IRB Barcelona, CRG (Barcelona), EPFL (Lausanne), ETH (Zuerich), DZNE (Bonn), Institut Curie (Paris). If BioImage Analysis is key to your research, do not miss this unique event to commit yourself in boosting the accessibility to BioImage Analysis tools and strengthening the community. Sincerely, Kota Miura, Centre for Molecular and Cellular Imaging, EMBL Heidelberg Jason Swedlow, Univ. of Dundee, Data Management and Analysis Work Package, EuroBioImaging Julien Colombelli & Sébastien Tosi, Advanced Digital Microscopy, IRB Barcelona To contact us: eubias2013(a)gmail.com <mailto:eubias2013@gmail.com> -- European BioImage Analysis Symposium - Barcelona 7 to12th of October http://eubias2013.irbbarcelona.org Organization Committee: Julien Colombelli - IRB Barcelona - julien.colombelli(a)irbbarcelona.org <mailto:julien.colombelli@irbbarcelona.org> (tel: +34 93 4020451) Sébastien Tosi - IRB Barcelona - sebastien.tosi(a)irbbarcelona.org <mailto:sebastien.tosi@irbbarcelona.org> Kota Miura - EMBL - kota.miura(a)embl.de <mailto:kota.miura@embl.de> Jason Swedlow - Univ of Dundee, Data Management and Analysis WP, EuBI

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[FILM-Users 00397] Fwd: Re: mNeonGreen Licensing
by Martin Spitaler 29 Jul '13

29 Jul '13

Dear microscopists, Birgit Leitinger is interested in buying the constructs for mNeonGreen, a fluorescent protein described recently in Nature Methods as particularly bright and also monomeric (http://www.nature.com/nmeth/journal/v10/n5/full/nmeth.2413.html) However, the company (http://www.allelebiotech.com/mneongreen) have an unusual licensing strategy, to individual labs - if enough labs are interested, the costs would go down from $600 to $100 per lab. Please let us know if this would be interesting for you. Good imaging, Martin *From: *Abbas Hussain <abbashussain(a)allelebiotech.com <mailto:abbashussain@allelebiotech.com>> *Date: *Wednesday, 22 May 2013 23:49 *To: *"Leitinger, Birgit" <b.leitinger(a)imperial.ac.uk <mailto:b.leitinger@imperial.ac.uk>> *Subject: *mNeonGreen Licensing Dear Birgit, Thank you for contacting us regarding mNeonGreen. The price for a single basic license is $300, and for the single advanced license it’s $600. Unfortunately we cannot accommodate a partial department, but if you can get the whole department on board it’s $100 per lab. Additionally, if you pay $600 now, and in a few months the department goes for the group license we can credit the $600 back to you. For ordering you can place the order on our website or call it in. You mentioned a quote in your email, do you know which option you would like it for, or did you need two separate quotes (one for each option)? Please let me know what I can do/provide you with to help you move forward in this process. Additionally if you have any questions please feel free to let me know. Sincerely, Abbas Hussain Director of Business Development Allele Biotech 6404 Nancy Ridge Dr. San Diego, CA 92121 AbbasHussain(a)allelebiotech.com <http://www.allelebiotech.com:2095/3rdparty/squirrelmail/src/compose.php?sen…> (858)587-6645 www.allelebiotech.com <http://www.allelebiotech.com/> -- ------------------------------------------------------------------------ *Martin Spitaler, PhD* *FILM **- Facility for Imaging by Light Microscopy* - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk <mailto:m.spitaler@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

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[FILM-Users 00396] Fwd: Invitation to the inaugural lecture of Professor Peter Kohl | 16 September 2013
by Martin Spitaler 25 Jul '13

25 Jul '13

...forwarded for your information - this is going to be an exciting presentation packed with the most spectacular imaging techniques, all the way to "Temporal pixel multiplexing" and "Measurement and analysis of sarcomere length in rat cardiomyocytes in situ and in vitro" (seePeter Kohl's website <http://www1.imperial.ac.uk/medicine/people/p.kohl/> for details)... Martin -------- Original Message -------- Subject: Invitation to the inaugural lecture of Professor Peter Kohl | 16 September 2013 Date: Thu, 25 Jul 2013 10:38:37 +0100 From: O'Donnell, Ruth A <r.odonnell(a)imperial.ac.uk> To: Faculty of Medicine, NHLI <icsm-nhli-dl(a)imperial.ac.uk> imap://mspitale@exchange.imperial.ac.uk:993/fetch%3EUID%3E/INBOX%3E170250?part=1.2&filename=image001.jpg The honour of your company is requested at an inaugural lecture: "Exploring the amazing heart: devil in detail, heaven in integration" *Professor Peter Kohl, **Chair in Cardiac Biophysics and Systems Biology* *Date*: Monday 16 September 2013 *Time*: 17.30 -- 18.30 *Venue*: Lecture Theatre G16, Sir Alexander Fleming Building, South Kensington Campus (building no 33 on the map <http://workspace.imperial.ac.uk/campusinfo/public/sthkencampus.pdf>). Travel information for the South Kensington Campus can be found on the Imperial website <http://www3.imperial.ac.uk/campusinfo/southkensington>. A pre-lecture tea will take place from 16.45 on the first floor concourse, Sir Alexander Fleming Building. ** *RSVP: **Ruth O'Donnell *r.odonnell(a)imperial.ac.uk <mailto:r.odonnell@imperial.ac.uk>** imap://mspitale@exchange.imperial.ac.uk:993/fetch%3EUID%3E/INBOX%3E170250?part=1.3&filename=image002.png imap://mspitale@exchange.imperial.ac.uk:993/fetch%3EUID%3E/INBOX%3E170250?part=1.4&filename=image003.jpg Scientifically viewed, the heart is a chemically powered, electrically controlled, mechanical pump, but the way in which it performs is nothing short of amazing. Heart structure and function are tightly interrelated in ways that we are still discovering. Cardiac muscle hosts a multitude of complex regulatory mechanisms that allow the heart to perform even after transplantation into another body and multiple feedback mechanisms provide the heart with an astonishing ability to adapt to the body's constantly changing demand in blood circulation. This happens during every heartbeat and roughly a million times during every ten-day period of our life. Given the importance of cardiac activity, it is surprising how many aspects of the heart are still poorly understood. This lecture will show that linking scientific observations of structure and function from sub-cellular scales to the whole body is essential in driving fundamental research and clinical application. imap://mspitale@exchange.imperial.ac.uk:993/fetch%3EUID%3E/INBOX%3E170250?part=1.5&filename=image004.jpg Peter Kohl holds the Chair in Cardiac Biophysics and Systems Biology at Imperial's National Heart and Lung Institute. He also is a visiting professor at the Department for Computer Science at the University of Oxford. Peter studied Medicine and Biophysics at the Moscow Pirogov Institute and after post-graduate training and research at the Berlin Charité he joined the Cardiac Electrophysiology Chair of Professor Denis Noble at the University of Oxford. In 1998, Peter set up the Oxford Cardiac Mechano-Electric Feedback lab, initially as a Royal Society Research Fellow and subsequently as a Senior Fellow of the British Heart Foundation. Peter's team enjoys a strong international reputation in cardiac mechano-electrical interaction studies as a result of their ability to cross traditional boundaries between fields (engineering, biophysics, biology, computing) and levels (ion channel to whole organ) of investigation. Peter directs a portfolio of externally-funded research (supported by ERC, BHF, BBSRC, EPSRC and EC), and he has been a driver of international collaborations such as the Network of Excellence for the EU Virtual Physiological Human Initiative. He serves on a number of editorial boards, as a reviewer for international journals and funding bodies and is the coordinating editor of the primary textbook on /Cardiac Mechano-Electric Coupling and Arrhythmias/. imap://mspitale@exchange.imperial.ac.uk:993/fetch%3EUID%3E/INBOX%3E170250?part=1.6&filename=image005.gif imap://mspitale@exchange.imperial.ac.uk:993/fetch%3EUID%3E/INBOX%3E170250?part=1.7&filename=image006.gif*Contact |* <mailto:events@imperial.ac.uk> imap://mspitale@exchange.imperial.ac.uk:993/fetch%3EUID%3E/INBOX%3E170250?part=1.7&filename=image006.gif *Campus Map |* <http://www3.imperial.ac.uk/campusinfo> imap://mspitale@exchange.imperial.ac.uk:993/fetch%3EUID%3E/INBOX%3E170250?part=1.7&filename=image006.gif *Coming to an Event * <http://www3.imperial.ac.uk/events/comingtoanevent> Stay informed with more Imperial College London News and Events: imap://mspitale@exchange.imperial.ac.uk:993/fetch%3EUID%3E/INBOX%3E170250?part=1.7&filename=image006.gif Events e-Bulletin | <http://www3.imperial.ac.uk/communications/bulletin> imap://mspitale@exchange.imperial.ac.uk:993/fetch%3EUID%3E/INBOX%3E170250?part=1.7&filename=image006.gif News e-Bulletin | <http://www3.imperial.ac.uk/media/dailynewsdigest> imap://mspitale@exchange.imperial.ac.uk:993/fetch%3EUID%3E/INBOX%3E170250?part=1.7&filename=image006.gif RSS Feeds | <http://www3.imperial.ac.uk/college/aboutthissite/rss> imap://mspitale@exchange.imperial.ac.uk:993/fetch%3EUID%3E/INBOX%3E170250?part=1.7&filename=image006.gif Online Lectures | <http://www3.imperial.ac.uk/college/media/onlinelectures> imap://mspitale@exchange.imperial.ac.uk:993/fetch%3EUID%3E/INBOX%3E170250?part=1.7&filename=image006.gif Monthly Podcast | <http://www3.imperial.ac.uk/college/media/podcasts> imap://mspitale@exchange.imperial.ac.uk:993/fetch%3EUID%3E/INBOX%3E170250?part=1.7&filename=image006.gif Reporter | <http://www3.imperial.ac.uk/reporter> imap://mspitale@exchange.imperial.ac.uk:993/fetch%3EUID%3E/INBOX%3E170250?part=1.7&filename=image006.gif Imperial Twitter <http://twitter.com/imperialcollege>

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[FILM-Users 00395] Fwd: Mouse Model Imaging
by Martin Spitaler 24 Jul '13

24 Jul '13

...forwarded for your information, Martin -------- Original Message -------- Subject: Mouse Model Imaging Date: Wed, 24 Jul 2013 11:21:29 +0100 From: Kurt I Anderson <k.anderson(a)beatson.gla.ac.uk> Dear Colleagues, I hope this meeting will be of interest to you, or someone you know. Registration has been extended for the upcoming Beatson Workshop on Preclinical Cancer Imaging*,* to be held September 1st - 3rd, 2013 at the Beatson Institute for Cancer Research in Glasgow, Scotland. Mouse models of disease are increasingly important tools at the intersection of cell biology and clinical medicine. The aim of this workshop is to promote the discussion and sharing of ideas focussed on the preclinical use of optical and nuclear imaging, technology development, and pancreatic cancer imaging. Beatson Cancer Workshops typically comprise 15 invited speakers, with another 10 selected from abstracts. The number of attendees is limited, and there will be plenty of time for discussion and interaction among the participants in an informal atmosphere. Please note that *all meals and two nights accommodation* are included in the registration price. There is also a reduced rate for local applicants who do not require accommodation. For more information and to register please visit: http://www.beatson.gla.ac.uk/Beatson-Workshop.html Best Begards, Kurt . ps: apologies if you receive this twice. Prof. Kurt I. Anderson Tumor Cell Migration Lab and Beatson Advanced Imaging Resource (BAIR) The Beatson Institute for Cancer Research Garscube Estate, Switchback Road, Glasgow G61 1BD (*Direct Line*+44 (0) 141 330 2864 (*Fax *+44 (0) 141 942 6521 ** E-mail k.anderson(a)beatson.gla.ac.uk <mailto:k.anderson@beatson.gla.ac.uk>* A company limited by guarantee; Registered in Scotland No 84170; A Registered Scottish Charity No SC006106 The information contained in this communication may be privileged and confidential and is intended for the exclusive use of the addressee designated above. If you are not the addressee, any disclosure, reproduction, distribution or other dissemination or use of this communication is strictly prohibited. If you have received this transmission in error please would you be kind enough to inform us.

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[FILM-Users 00394] Fwd: thanks FILM!
by Martin Spitaler 24 Jul '13

24 Jul '13

Dear microscopists, quite timely for our grant application for a new super-resolution microscope (and just in time before the deadline), the first super-resolution paper from our home-built system has been published, with Steve Rothery as co-author (thanks Sophie and Dan, and congratulations to all authors) - and it made it straight to the Science Signalling frontpage (with an image of the result of Dylan Owen's clustering algorithm; Dylan did his PhD here and is now in Kings College)! So let's hope our application for a dual PALM/STORM + SIM microscope will be successful, so we (FILM + users) can build on this success. Good imaging, Martin <http://stke.sciencemag.org/content/vol6/issue285/cover.dtl> -------- Original Message -------- Subject: thanks FILM! Date: Wed, 24 Jul 2013 10:21:56 +0100 From: Davis, Daniel M <d.davis(a)imperial.ac.uk> To: Spitaler, Martin <m.spitaler(a)imperial.ac.uk> Hi Martin, I hope all's well. Our new paper - by Sophie, featuring FILM and with Stephen as a co-author - is just out today. If useful, please add to the FILM web-page and perhaps send it to your email list? Paper is here: http://stke.sciencemag.org/cgi/content/abstract/sigtrans;6/285/ra62 It's also on the front of the Manchester Uni web page: http://www.manchester.ac.uk/aboutus/news/display/?id=10446 <http://www.manchester.ac.uk/aboutus/news/display/?id=10446> and there's a podcast about it here: http://stke.sciencemag.org/cgi/content/full/sigtrans;6/285/pc19 Cheers, dan Dan Davis Professor of Molecular Immunology Lab Web Page: http://www.dandavislab.co.uk/ Book: http://t.co/A0A9810w Cover Figure

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[FILM-Users 00393] Fwd: PDRA post developing motion-stablised hand-held multiphoton scanner for clinical applications
by Martin Spitaler 23 Jul '13

23 Jul '13

...forwarded for your information, Martin -------- Original Message -------- Subject: PDRA post developing motion-stablised hand-held multiphoton scanner for clinical applications Date: Thu, 11 Jul 2013 17:20:46 +0000 From: Dunsby, Christopher W <christopher.dunsby(a)IMPERIAL.AC.UK> Reply-To: Dunsby, Christopher W <christopher.dunsby(a)IMPERIAL.AC.UK> To: <UK-EUROBIOIMAGING-PROJECT(a)JISCMAIL.AC.UK> Imperial College London is currently recruiting a postdoctoral researcher to develop a motion-stablised hand-held multiphoton scanner for clinical applications. The post is available for up to 2 years. This is a full-time post funded by the Engineering and Physical Sciences Research Council as part of a multidisciplinary research project to develop and apply novel approaches for /in vivo/ multiphoton microscopy of tissue autofluorescence for clinical applications. The successful applicant will have a background in fluorescence imaging technology, preferably with experience of multiphoton microscopy, designing optical systems, working with single mode and multimode optical fibres, and working with and aligning ultrafast lasers. Their role will be to develop a novel hand-held motion-stabilised multiphoton microscopy system for clinical applications. This will include the development of appropriate hardware and software, its implementation for /in vivo/ imaging and an evaluation of its performance. For more details visit www.imperial.ac.uk/employment <http://www.imperial.ac.uk/employment> - click job search and enter NS2013134NT in "keywords". -------------------------------- Dr Chris Dunsby Joint Lecturer between Photonics, Department of Physics & Division of Experimental Medicine Photonics Department of Physics South Kensington Campus Imperial College London London SW7 2AZ +44 (0) 20 7594 7755 (x47755) -- UK-EUROBIOIMAGING Visit the website http://www.bioimaginguk.org To e-mail the group, send messages to UK-EUROBIOIMAGING-PROJECT(a)JISCMAIL.AC.UK To subscribe visit https://www.jiscmail.ac.uk/cgi-bin/webadmin?A0=UK-EUROBIOIMAGING-PROJECT --

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