- FILM-Users - imperial.ac.uk

[FILM-Users 00469] Fwd: PhD Studentships
by Martin Spitaler 10 Feb '14

10 Feb '14

...forwarded for your information, Martin -------- Original Message -------- Subject: PhD Studentships Date: Fri, 7 Feb 2014 13:31:14 +0000 From: Alison Dun <manager(a)esric.org> To: Undisclosed recipients:; Dear All, I am the facility manager for ESRIC, a super-resolution imaging facility here in Edinburgh. I attended the UK facility managers meeting in Leeds this January and met some of you there. I am writing to ask if you wouldn't mind circulating some PhD opportunities that are available through ESRIC, the details are below. Thank you in advance for taking the time, Ali Dr. Alison Dun Technology and Facility Manager Edinburgh Super Resolution Imaging Consortium (ESRIC) Heriot-Watt University Edinburgh EH14 4AS Tel: +44 7977 518 581 http://www.esric.org / / /A number of three year prize PhD studentships are available in The Edinburgh Super-Resolution Imaging Consortium (ESRIC) using state-of-the-art microscopy techniques. Full details can be found through the links below,/ / / /http://www.findaphd.com/search/PhDDetails.aspx?CAID=1143/ / / /For more information about the projects offered please visit:/ / / /http://www.esric.org/opportunities.html/ / / /This is an exciting opportunity to work at the cutting edge of biological imaging at the molecular scale with access to the latest technology and expertise across the scientific disciplines. Appropriate multi-disciplinary supervision will be provided. / / / /PLEASE NOTE that there are eligibility criteria for these studentships relating to nationality, and period and purpose of residency in the UK. Only EU nationals are eligible, but there are further restrictions for non-UK nationals./

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[FILM-Users 00468] COMMERCIAL FW: Introducing Click-iT(R) Plus EdU kits for Flow Cytometry, RPE and GFP compatible!
by Martin Spitaler 10 Feb '14

10 Feb '14

COMMERCIAL Fwd: *From:*Buck, Suzanne [mailto:Suzanne.Buck@thermofisher.com] *Sent:* 07 February 2014 11:44 *To:* Simpson, Catherine A *Subject:* Introducing Click-iT(R) Plus EdU kits for Flow Cytometry, RPE and GFP compatible! Hello Catherine, I'm writing to let you know that the new Click-iT® Plus EdU kits for flow cytometry are now available. Unlike the original kits, these are fully compatible with GFP, R-PE and R-PE tandems. Please click on the skus below to see additional information about the products. The Pacific Blue^(TM) dye product for flow cytometry will be released at a later date. As with the original kits, we also have imaging kits, and Tool Kits available in the new Click-iT® Plus EdU format as well. Flow Cytometry Kits C10632 <https://www.lifetechnologies.com/order/catalog/product/C10632> Click-iT® Plus EdU Alexa Fluor® 488 Flow Cytometry Assay Kit *50 assays* C10633 <https://www.lifetechnologies.com/order/catalog/product/C10633> Click-iT® Plus EdU Alexa Fluor® 488 Flow Cytometry Assay Kit *100 assays* C10634 <https://www.lifetechnologies.com/order/catalog/product/C10634> Click-iT® Plus EdU Alexa Fluor® 647 Flow Cytometry Assay Kit *50 assays* C10635 <https://www.lifetechnologies.com/order/catalog/product/C10635> Click-iT® Plus EdU Alexa Fluor® 647 Flow Cytometry Assay Kit *100 assays* C10636 Click-iT® Plus EdU Pacific Blue^(TM) Flow Cytometry Assay Kit -- */coming soon/* Imaging Kits C10637 <https://www.lifetechnologies.com/order/catalog/product/C10637> Click-iT® Plus EdU Alexa Fluor® 488 Imaging Kit *for 50 coverslips* C10638 <https://www.lifetechnologies.com/order/catalog/product/C10638> Click-iT® Plus EdU Alexa Fluor® 555 Imaging Kit *for 50 coverslips* C10639 <https://www.lifetechnologies.com/order/catalog/product/C10639> Click-iT® Plus EdU Alexa Fluor® 594 Imaging Kit *for 50 coverslips* C10640 <https://www.lifetechnologies.com/order/catalog/product/C10640> Click-iT® Plus EdU Alexa Fluor® 647 Imaging Kit *for 50 coverslips* Toolbox Kits C10641 <https://www.lifetechnologies.com/order/catalog/product/C10641> Click-iT® Plus Alexa Fluor® 488 picolyl azide toolkit C10642 <https://www.lifetechnologies.com/order/catalog/product/C10642> Click-iT® Plus Alexa Fluor® 555 picolyl azide toolkit C10643 <https://www.lifetechnologies.com/order/catalog/product/C10643> Click-iT® Plus Alexa Fluor® 647 picolyl azide toolkit The tool kits contains everything to perform copper-catalyzed click reactions with the ability to moderate the level of free copper while retaining all of the benefits of standard azide/alkyne click reaction. Scientists who label DNA, RNA, peptides or proteins in live cells, whole animals or in vitro using Click chemistry, should consider these products. If you have any questions, please let me know. Kindest Regards, Suzanne *Suzanne B. Buck* Product Manager Flow Cytometry Reagents Life Sciences Solutions Thermo Fisher Scientific 29851 Willow Creek Road Eugene, OR 97402 USA Direct: (541) 335-0037 Mobile: (541) 285-6113 suzanne.buck(a)thermofisher.com <mailto:suzanne.buck@thermofisher.com> lifetechnologies.com

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[FILM-Users 00467] Fwd: Invitation To InSphero 3D Microtissues Meeting - 12th March 2014
by Martin Spitaler 10 Feb '14

10 Feb '14

...forwarded for your information, Martin -------- Original Message -------- Subject: Invitation To InSphero 3D Microtissues Meeting - 12th March 2014 Date: Thu, 6 Feb 2014 08:22:27 -0600 From: Mike Jowett <mike.jowett(a)bioscience.co.uk> Reply-To: support(a)bioscience.co.uk <reply-fe5c1773776007747c16-14763377_HTML-224577665-77388-0(a)reply.bioscience.co.uk> To: <m.spitaler(a)imperial.ac.uk> To view this email as a web page, go here. <http://cl.exct.net/?ju=fe38107075640579701174&ls=fdee1d7675630c7873127174&m…> InSphero Header <http://cl.exct.net/?ju=fe37107075640579701175&ls=fdee1d7675630c7873127174&m…> *InSphero 3D Microtissues Meeting Invitation* /*3D Cell-Based Assays In Predictive Toxicological Studies Friday 12th March 2014*/ Dear Dr Spitaler, I would like to personally invite you to attend the *InSphero 3D Microtissues* *Meeting* on *Friday 12th March 2014*. Entitled *3D Cell-Based Assays In Predictive Toxicological Studies* and held in *The Trinity Centre* on the *Cambridge Milton Road Science Park*, this *free event* begins at *8:30am* with a light breakfast and finishes with a lunch at *1:30pm*. *Focus Of This Meeting* Classic 2D cell culture systems alone have been shown not to offer the predictive power needed to make confident decisions for lead compound prioritization or to perform in-depth investigational toxicity studies. This workshop aims to bring together researchers and industry experts involved directly with the *use* and *development *of *3D microtissues* for *safety, toxicology testing, research* and *drug discovery* applications, as well as those considering using 3D microtissues in the future. *Agenda** *Th*e *agenda of this half day meeting will cover a range of interesting applications and innovations in the use of the 3D microtissues, enabling the exchange of valuable information between both existing and future users. Speakers and talks include: *A Comparative Insight Into Liver Nanotoxicology Utilising 3D InSight^(TM) Human Liver Microtissue, Ex Vivo Liver Cells & An /In Vivo/ Intravenous Nanoparticle Exposure Model*/ Ali Kermanizadeh, Copenhagen University/ HepG2 Liver Microtissues <http://cl.exct.net/?ju=fe36107075640579701176&ls=fdee1d7675630c7873127174&m…> *Improving Drug Safety with Advanced 3D Human Liver Models* //Stephanie Urschel, Pharmacelsus GmbH/ /*Global Proteomic Analysis Of Drug Induced Toxicity In 3D Human Liver Microtissues*/ /Claudia Escher, Biognosys AG/ / *Organotypic 3D Cell Culture Models For Drug De-Risking* //Jens Kelm, InSphero AG/// / *The Use Of 3D Human Liver Microtissues For Hepatotoxicity Testing*// Simon Messner, InSphero AG/ /*Book Your Place* If you would like to reserve your place at this exciting event, please *Click Here <http://cl.exct.net/?ju=fe35107075640579701177&ls=fdee1d7675630c7873127174&m…>* and complete the registration form or phone *+44 (0)1223 316855.* *About InSphero* InSphero is the leading, award-winning supplier of assay-ready 3D microtissues for drug discovery and pre-clinical testing, helping you to improve data relevance and to reduce the overall cost of drug development. To find out more, please *Click Here* <http://cl.exct.net/?ju=fe34107075640579701178&ls=fdee1d7675630c7873127174&m…> If you have any questions or would like to discuss the InSphero technology or 3D microtissues further, please do not hesitate to contact me. Otherwise, I look forward to welcoming you to our meeting in March. Best Regards, /Mike/ *Mike Jowett* Business Manager Cambridge Bioscience Limited Munro House Trafalgar Way Bar Hill Cambridge CB23 8SQ United Kingdom *Tel: *+44 (0)1954 782 596 ext. 227 *Mob:*+44 (0)7795 514 139 *Email: */mike.jowett(a)bioscience.co.uk <mailto:%20mike.jowett@bioscience.co.uk> //www.bioscience.co.uk <http://cl.exct.net/?ju=fe33107075640579701179&ls=fdee1d7675630c7873127174&m…>/ InSphero Footer <http://cl.exct.net/?ju=fe3b107075640579701270&ls=fdee1d7675630c7873127174&m…> This email was sent to: *m.spitaler(a)imperial.ac.uk* This email was sent by: Cambridge Bioscience Munro House, Bar Hill, Cambridge, Cambs, CB23 8SQ, United Kingdom We respect your right to privacy - view our policy <http://cl.exct.net/?ju=fe3a107075640579701271&ls=fdee1d7675630c7873127174&m…> <http://cl.exct.net/?ju=fe39107075640579701272&ls=fdee1d7675630c7873127174&m…> Update Profile and Subscription Preferences <http://cl.exct.net/?ju=fe38107075640579701273&ls=fdee1d7675630c7873127174&m…> | Unsubscribe From All Emails <http://cl.exct.net/?ju=fe37107075640579701274&ls=fdee1d7675630c7873127174&m…>

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[FILM-Users 00466] Fwd: Join our free webinar: Latest Biological Applications for 3D Super-resolution Microscopy
by Martin Spitaler 04 Feb '14

04 Feb '14

...forwarded for your information, Martin -------- Original Message -------- Subject: Join our free webinar: Latest Biological Applications for 3D Super-resolution Microscopy Date: Mon, 3 Feb 2014 16:03:18 +0100 From: Leica Microsystems <news.eu(a)leica-microsystems.com> Reply-To: Leica Microsystems <nina.brauns(a)leica-microsystems.com> To: <m.spitaler(a)imperial.ac.uk> Click here to view this e-mail as webpage <http://rdir.inxmail.com/leica/d?q000nmoi00s46s00d0000000000000000cttcn3i38&…> <http://rdir.inxmail.com/leica/d?q000nmqi00s46s00d0000000000000000cttbzki38&…> Dear Scientist, *FREE Online Web Seminar** Wednesday February 19, 2014* 08:00 PST | 11:00 EST | 16:00 GMT | 17:00 CET *Click here to register* <http://rdir.inxmail.com/leica/d?q000nmq000s46s00d0000000000000000cttkus038&…> Leica Microsystems, in conjunction with Microscopy and Analysis, are pleased to announce a webinar highlighting ground-breaking techniques in microscopy. Dr. Timo Zimmermann and Dr. Eric Hosy will present the impressive results they have achieved using confocal and widefield 3D super-resolution methods. Biological application for the next generaton of STED microscopy systems Stimulated Emission Depletion (STED) constitutes a powerful method for resolution improvement beyond the diffraction in light microscopy. Additional time-gating (gSTED) of the detection signal can be used in systems with continuous wave (CW) laser depletion to improve the image resolution even further. Until now, these improvements were limited in gated CW-STED systems to the lateral resolution of the image and to the depletion of green fluorophores at 592nm. The now introduced possibility to improve axial resolution and the introduction of an additional laser line for depletion at 660nm offer new ways to image biological samples. We have taken highly resolved 3D and multichannel datasets of a variety of specimens and structures to make full use of these new features. In the talk, several projects will be covered that make use of the improved z-resolution and that use orange to red dyes and dye combinations for the new depletion laser line. <http://rdir.inxmail.com/leica/d?q000nmqi00s46s00d0000000000000000cttbzki38&…> *Speaker: Dr. Timo Zimmermann*, Head of the Advanced Light Microscopy Unit, CRG-Centre for Genomic Regulation, Spain Live cell localization microscopy with Leica SR GSD 3D Brownian diffusion tends to homogenize proteins that should diffuse rapidly inside the cytoplasm or cell surface. Protein clustering or immobilization necessitates specific molecular organization which counteracts this phenomenon. During the last ten years, knowledge about the role and properties of protein diffusion made a big step forward thanks to the development of single molecule and Quantum dot tracking (SPT). However the sparse labelling inherent to these techniques render it impossible to give an overview of the protein organization. We will report, in this webinar, the historic, the interest and the proper use of a new optical technique allowing users to obtain a high number of single molecule trajectories at the surface of a living cell using the Leica SR GSD 3D system. This versatile method, developed in 2010 and called U-PAINT, is suitable for studying any membrane biomolecule (endogenous or transfected) and provides dynamical information with large statistics revealing localization specific diffusion properties of a molecule on a single cell. <http://rdir.inxmail.com/leica/d?q000nmqi00s46s00d0000000000000000cttbzki38&…> *Speaker: Dr. Eric Hosy*, CNRS, University of Bordeaux, France Imprint Leica Mikrosysteme Vertrieb GmbH, Ernst-Leitz-Straße 17-37, 35578 Wetzlar Phone: +49 6441 29-4000, Fax: +49 6441 29-4155, Website: www.leica-microsystems.com Email: news_eu(a)leica-microsystems.com Managing Director: Frank Bunge, Colin Davis, Cornelis Johannes Maria van Ophem Trade register number: Wetzlar HRB 5464, VAT number: DE 811140297 Leica Microsystems owns the copyright and is responsible for all contents and data. Subsequent utilization of text, data and pictures only with prior permission. Click here <http://rdir.inxmail.com/leica/d/d.php?q000nmny00s46s00k0000000000000000ctt0…> if you want to unsubscribe. <http://rdir.inxmail.com/leica/d?q000nmpi00s46s00d0000000000000000cttpd2y38&…> <http://rdir.inxmail.com/leica/d?q000nmpq00s46s00d0000000000000000cttczci38&…> <http://rdir.inxmail.com/leica/d?q000nmpy00s46s00d0000000000000000cttju2038&…>

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[FILM-Users 00465] new FEC rates for grant applications
by Martin Spitaler 03 Feb '14

03 Feb '14

Dear microscopists, please note that the Research Support Services have done their *annual update of the FEC rates *for facility usage, effective from 1st of February 2014. These are the rates to be used on grant applications, calculated from the full running costs, divided by the hours of usage for each microscope category. Because these rates are based on future estimates, the actual, current charge-out rates might slightly vary, and with good usage hopefully be lower. The current charging rates will be calculated now and updated on 1st of April. *Important*: The calculation is obvious, costs divided by hours, so the more you use the microscopes, the cheaper they get! We don't make any profit, and we'd very much prefer the microscopes used by twice as many users for half the rate than the other way round. The more they are used, the more great science is done for lower costs, with good usage rates will go down, it has happened several times in the past. So don't try to save money in the wrong place, make the best of the excellent equipment and hopefully similar support (if there is something we can improve, please let us know!). And please get in touch if you need any help with your grant applications. We are very happy to advice on any microscopy-related issues, fill in technical parts, sample preparation, data analysis etc. A detailed project plan can only help with your success rate. All the information about grant applications and costings can be found on the Research Support Services on their website <http://www3.imperial.ac.uk/researchsupport/costingandpricing/costing#resear…>. Hourly rates for FILM usage are published in the document <Charge Out List Publication.pdf <https://workspace.imperial.ac.uk/researchservices/Internal/Charge%20Out%20L…>>. Good imaging, Martin -- ------------------------------------------------------------------------ *Martin Spitaler, PhD* *FILM - Facility for Imaging by Light Microscopy* - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk <mailto:m.spitaler@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

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[FILM-Users 00464] lists of fluorescent proteins
by Martin Spitaler 31 Jan '14

31 Jan '14

Dear microscopists, we frequently get requests for proteins for special applications, like intravital imaging (far-red), sensing (binding specificity), super-resolution (photo-switching) etc. I have just added two excellent, interactive lists to the FILM website > LINKS > GENERAL INFORMATION <http://www3.imperial.ac.uk/portal/page/portallive/imagingfacility/links/ima…>, both hosted at UCSF: * Fluorescent proteins <http://nic.ucsf.edu/FPvisualization/> * Photoswitchable proteins <http://nic.ucsf.edu/FPvisualization/PSFP.html> Worth having a look, there is much more to life (science research) than GFP and CFP. Good imaging, Martin -- ------------------------------------------------------------------------ *Martin Spitaler, PhD* *FILM - Facility for Imaging by Light Microscopy* - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk <mailto:m.spitaler@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

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[FILM-Users 00463] Definiens Advanced training course
by Martin Spitaler 31 Jan '14

31 Jan '14

Dear microscopists, there are still some places left on the DEFINIENS ADVANCED TRAINING COURSE will take place Wed-Fri 5-7 March 2014 in the computer training room of the Central Library. The training will be customised to our needs, the *confirmed topics* (responding to you users) will be: * tracking * machine learning * Image registration * Feature space optimization If interested, please get in touch as soon as possible. And don't forget, this is offered at a discounted rate (thanks to the Imperial College Equipment Sharing grant) of only £400 per person! Good imaging, Martin -- ------------------------------------------------------------------------ *Martin Spitaler, PhD* *FILM - Facility for Imaging by Light Microscopy* - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk <mailto:m.spitaler@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

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[FILM-Users 00462] Fwd: FW: Image competition deadline 1st Feb!!!
by Martin Spitaler 30 Jan '14

30 Jan '14

...forwarded for your information, Martin -------- Original Message -------- Subject: FW: Image competition deadline 1st Feb!!! Date: Thu, 30 Jan 2014 10:37:36 +0000 From: Braga, Vania M M <v.braga(a)imperial.ac.uk> * Subject:* Image competition deadline 1st Feb!!! *Importance:* High Dear all Sorry to hassle you again, but we urgently need submissions for the image competition whose deadline is 1 Feb. So far I only have one entry and that was at the wrong size/resolution. Usually we get 10-20 entries, so I'm unsure what has gone wrong this year. The winners get cash prizes, will appear on the cover of the newsletter and on the new website where they will be featured in a dedicated gallery. *Entry is open to any BSCB member (including lab heads! ) Copyright is retained by the entrant. * *http://www.bscb.org/imagecompetition* * * * * *Also don't forget to encourage your postdoc and lab members to submit a short piece of text for the Writing Prize! * *http://www.bscb.org/sciencewriting* Many thanks Paul ________________________________________________ Dr. Paul. D. Andrews BSc PhD FSB BSCB Public Engagement Coordinator Mobile +44 7590543266 email:pdandrews1@mac.com

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[FILM-Users 00461] TODAY: FILM Club Wed 29 Jan, SAFB-122: Catherine Simpson - Sort your microscopy out!
by Martin Spitaler 29 Jan '14

29 Jan '14

Just a reminder: ------------------------------------------------------------------------ Dear microscopists, the first FILM Club of the new year is coming up next *TODAY*: *Catherine Simpson: "Sort your microscopy out!" Wed 29 Jan, SAFB-122, 4.30pm* Catherine Simpson is the facility manager of the Flow Cytometry Facility in South Kensington (http://www3.imperial.ac.uk/lifesciences/research/facilities/flowcytometry) In this talk, she will go into the very powerful combination of flow cytometry and microscopy to extract much more useful information from biological samples. While flow cytometry is incredibly powerful in quantifying multiple cellular parameters at high speed from large numbers of samples, it's missing one important trick: The cellular context of these parameters in time and space. This is where microscopy excels, it keeps the whole big picture intact, how molecules relate to each other, interact with each other and change over time. But this comes at the price of often lousy statistics, especially for live imaging. And it can take ages to find the 'needle in the haystack', for example a cell of a certain type, transiently transfected or with a certain expression level or combination or fluorophores. Combining the two - analysis and sorting on a FACS, live visualisation and measurement on a microscope - opens up a whole new dimension of understanding. Cells can be analysed in high numbers, rare cells sorted into microscopy multi-well plates or slides, or even re-introduced into live animals and followed up by intravital imaging. Some people ask for the ImageStream technology to analyse their samples - we can do all that and much more, at much higher magnification and over time! See you there, good imaging, Martin -- ------------------------------------------------------------------------ *Martin Spitaler, PhD* *FILM - Facility for Imaging by Light Microscopy* - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk <mailto:m.spitaler@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

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[FILM-Users 00460] TOMORROW: FILM Club Wed 29 Jan, SAFB-122: Catherine Simpson - Sort your microscopy out!
by Martin Spitaler 28 Jan '14

28 Jan '14

Just a reminder: ------------------------------------------------------------------------ Dear microscopists, the first FILM Club of the new year is coming up next *TOMORROW*: *Catherine Simpson: "Sort your microscopy out!" Wed 29 Jan, SAFB-122, 4.30pm* Catherine Simpson is the facility manager of the Flow Cytometry Facility in South Kensington (http://www3.imperial.ac.uk/lifesciences/research/facilities/flowcytometry) In this talk, she will go into the very powerful combination of flow cytometry and microscopy to extract much more useful information from biological samples. While flow cytometry is incredibly powerful in quantifying multiple cellular parameters at high speed from large numbers of samples, it's missing one important trick: The cellular context of these parameters in time and space. This is where microscopy excels, it keeps the whole big picture intact, how molecules relate to each other, interact with each other and change over time. But this comes at the price of often lousy statistics, especially for live imaging. And it can take ages to find the 'needle in the haystack', for example a cell of a certain type, transiently transfected or with a certain expression level or combination or fluorophores. Combining the two - analysis and sorting on a FACS, live visualisation and measurement on a microscope - opens up a whole new dimension of understanding. Cells can be analysed in high numbers, rare cells sorted into microscopy multi-well plates or slides, or even re-introduced into live animals and followed up by intravital imaging. Some people ask for the ImageStream technology to analyse their samples - we can do all that and much more, at much higher magnification and over time! See you there, good imaging, Martin -- ------------------------------------------------------------------------ *Martin Spitaler, PhD* *FILM - Facility for Imaging by Light Microscopy* - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk <mailto:m.spitaler@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

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