- FILM-Users - imperial.ac.uk

[FILM-Users 00595] movies for putative music video
by Keller, Debora 16 Oct '15

16 Oct '15

Dear Users, This is a bit out of the ordinary, but FILM has been contacted to help provide materials for a video for the band Mystery Jets<https://en.wikipedia.org/wiki/Mystery_Jets>. The producers are looking for movies of tissue, organisms, etc showing "growth", "accumulation" . Movies of growing plants, cell colonies, dancing cells, cells in zebrafish, accumulation of cells in tissue (cancer cells?) etc... could be useful. If you have movies you think could be used, please place them in my scratch folder "_DKeller Microscopes\_MYSTERY JETS by Monday evening. The folder also contains a synopsis of what the director would like to do - more artistic than scientific, but microscopy is art :) No specifics will be communicated so your science will be safe - if you have questions, contact me. I will show them to the producer and if they decide to choose one or the other, you will be appropriately acknowledged on the video. Cheers Debbi Mystery jets music: https://www.youtube.com/watch?v=BmkdGBM1x3M&list=PLB-ImgV-XxcCD3xYHbn9b_8_6… Debora Keller, PhD FILM - Facility for Imaging by Light Microscopy - Facility Manager - Super-Resolution Specialist - Sir Alexander Fleming Building, desk 408 Imperial College London / South Kensington Exhibition Road London SW7 2AZ, UK Phone: +44 (0)207 594 2023 Mobile: + 44(0) 7760 256 889 E-mail: d.keller(a)imperial.ac.uk<mailto:d.keller@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

1 0

[FILM-Users 00593] microscopes, liquids and oil
by Keller, Debora 16 Oct '15

16 Oct '15

Dear Users, The WF2 will be out of order until further notice. Attached you can see the reason why: massive corrosion at the bottom of the microscope. This means that liquid went all the way from the objective, the filter turret and ended up on the slider. This is most likely not due to a single incident. We are waiting to hear from Zeiss whether this can be repaired locally or whether the entire microscope has to be sent to Germany - which might not fall under our service warranty. To be seen... >> All WF2 users, please contact us (film-service(a)imperial.ac.uk<mailto:film-service@imperial.ac.uk>) so we can transfer you onto other machines. Similarly on CF4, a Leica engineer had to clean a mirror under the objective turret as it was full of oil. Had it not been spotted, it could have led to some more damage similar to WF2. In order to keep our microscopes functioning and our users happy, we need all of you to pay extra attention when handling liquids and oil: - Only put a small drop of oil on your coverslips, it is plenty. - remove the oil after you are done from all objectives where it was used and from your slide. - Be extra careful when handling liquids, plates or dishes with media, fluidics. - Always report a spill, as tiny as it might be - if unchecked some liquid can infiltrate the body of the microscope and lead to the corrorsion we saw on WF2. - When in doubt, ask us. Do not hesitate to ask us if you are unsure. Best wishes, Debbi Debora Keller, PhD FILM - Facility for Imaging by Light Microscopy - Facility Manager - Super-Resolution Specialist - Sir Alexander Fleming Building, desk 408 Imperial College London / South Kensington Exhibition Road London SW7 2AZ, UK Phone: +44 (0)207 594 2023 Mobile: + 44(0) 7760 256 889 E-mail: d.keller(a)imperial.ac.uk<mailto:d.keller@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

2 1

[FILM-Users 00592] URGENT QUICK RESPONSE NEEDED: Wellcome Trust Multi-User Equipment grant
by Keller, Debora 12 Oct '15

12 Oct '15

Dear microscopists, The Wellcome Trust has just opened a call for "Multi-user equipment grant" (deadline 20th November). We were successful in 2014 in obtaining it (upgrades to CF3/CF4, high-content imaging system & post for David) but think it might still be an excellent opportunity to further upgrade our facility to respond to your latest needs. The grant is intended to cover purchase and running costs of major equipment (up to £1 million, including up to 5 years running costs), which gives us an opportunity to cover unmet long-term needs ie any piece of equipment that would be essential for your research or enable you to do really cool experiments! We would like to have your input on: - the kind of experiments you would like to do in the future that you can't do currently in FILM, - where major investment would make a big difference to your research. We already have some ideas, in particular given that the LSM510 will come out of support from Zeiss and is fairly old, one piece of equipment we are thinking of applying for is the new Zeiss Airy scan confocal<http://www.zeiss.com/microscopy/en_us/products/confocal-microscopes/lsm-880…> (inverted) and/or upgrade the HCF1 with the Airy Scan detector. Previous ideas put up were: - lightsheet microscope, - intravital imaging - dedicated long-term imaging microscopes (e.g Nikon Biostation<http://nikon.com/about/technology/life/instruments/biostation/index.htm> or similar Incucyte, etc) - spinning disk confocal - flow imaging but feedback on any unmet needs is appreciated. Don't forget any microscopy-related needs such as data processing and image analysis. IMPORTANT: if you are a Wellcome Trust grant holder and would agree to be a co-applicant, this would greatly enhance our chances. The deadline is soon and it's a busy time of the year, so please let me know within a week what your thoughts are (please reply to me personally, not the whole mailing list). Looking forward to your input, good imaging, Debbi & Tony Links: * Wellcome Trust Multi-User Equipment Grants: < http://www.wellcome.ac.uk/Funding/Biomedical-science/Funding-schemes/Multi-…> * Facility for Imaging by Light Microscopy [FILM]: <http://www3.imperial.ac.uk/imagingfacility/> Debora Keller, PhD FILM - Facility for Imaging by Light Microscopy - Facility Manager - Super-Resolution Specialist - Sir Alexander Fleming Building, desk 408 Imperial College London / South Kensington Exhibition Road London SW7 2AZ, UK Phone: +44 (0)207 594 2023 Mobile: + 44(0) 7760 256 889 E-mail: d.keller(a)imperial.ac.uk<mailto:d.keller@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

1 0

[FILM-Users 00591] Hounsfield biomedical imaging poster deadline extended - Friday 16th October
by Keller, Debora 09 Oct '15

09 Oct '15

FYI: Dear colleagues The deadline for the Hounsfield Lecture biomedical imaging poster competition has been extended to Friday 16th October. Please submit your posters following the instructions on the Hounsfield Lecture website http://www.hounsfieldlecture.org/ Dear colleagues We are pleased to announce that the Hounsfield Lecture is being re-launched in memory of Nobel Laureate Sir Godfrey Hounsfield. This event brings together medical imaging researchers at ICL and beyond and allows an international leader in biomedical imaging to address the college imaging community. This year's Hounsfield lecture will be given by Professor Jürgen Hennig<https://www.bcf.uni-freiburg.de/people/details/hennig> from the University Medical Center Freiburg. Magnetic Resonance Imaging: Visions of Life Professor Jürgen Hennig Jürgen Hennig is a German chemist and medical physicist, internationally renowned for his work as one of the pioneers of Magnetic Resonance Imaging for clinical diagnostics. He is an alumnus of Imperial and also studied at the universities of Stuttgart, Munich and Freiburg, where he currently works as Scientific Director of the Department of Diagnostic Radiology and Chairman of the Magnetic Resonance Development and Application Center. His awards include the Biosciences and Medicine Max Planck Research Award (2003), the highest scientific award of Taiwan - Tsung Ming Tu Award (2010), and an Einstein professorship from the Chinese Academy of Science (2011). Please save the date, 6 pm Wednesday 4th November, and note the location will be SAF G16. Further details, including information on a medical imaging poster event and competition<http://hamlyn.doc.ic.ac.uk/hounsfield/poster-competition> to be held at 5 pm the same day can be found on the Hounsfield Lecture website - please submit a poster! http://www.hounsfieldlecture.org/ Yours sincerely Hounsfield Lecture Organising Committee 2015 Jürgen Hennig - Magnetic Resonance Imaging: Visions of Life Magnetic Resonance Imaging (MRI) has been around for more than 30 years but is still undergoing rapid development. This progress is being driven by innovative technologies as well as new challenges and opportunities arising from the rapid development in biomedical research. MRI not only produces good images, but it also enables non-invasive measurement and visualization of metabolic, microstructural, functional and physiological processes. These offer the potential for studying the multiple parameters associated with normal and disease processes, with applications to areas of medical research including neuroscience, cardiology and metabolic disease. Multiparametric MRI imaging provides an ideal and necessary supplement to genetic profiling and personalized medicine. In conjunction with other imaging techniques, MRI adds a spatiotemporal dimension to the spatially non-specific information provided by genetics. The importance of this can be seen through analysing the genetic heterogeneity of tumors, and MRI allows the detailed study of changes in phenotype caused by genetic modifications in animal models. The richness of phenotype modifications caused by even very isolated genetic changes is ample demonstration that the interrelation of molecular factors with the systemic level is much more than a simple one-to-one correspondence. The clever and creative use of new nano- and microtechnologies has already led to unprecedented improvements in imaging quality and imaging speed as well as new concepts in spatial encoding and the widespread use of high power graphical processor units. The introduction of new technologies is expected to lead to incremental improvements in current approaches to MRI, as well as new applications. Although MRI has come a long way since its early stages, it still has far to go. The Hounsfield Lecture Advances in biomedical imaging technology have allowed tremendous progress in diagnostic and surgical imaging since the first X-ray. Each new development has been based on fundamental scientific progress in science and engineering, resulting in Nobel prizes in medicine for the non-medically trained inventors of techniques such as CT and MRI. Activity that brings together scientists, engineers and clinicians can promote further technological breakthroughs. The Hounsfield Lecture is one such opportunity and allows an international leader in biomedical imaging to address the college imaging community. The event acts as a focus to bring together medical imaging researchers at ICL and beyond and features a poster viewing event and competition that attracts high numbers of submissions from across all faculties. The inaugural event was presented in 2005 and the last was in 2009. Past speakers at the event include James Fujimoto, Rakesh K Jain, Peter Hunter, Sam Gambhir and Robert Balaban.

1 0

[FILM-Users 00590] Imperial biological (pre-clinical) imaging centre
by Keller, Debora 09 Oct '15

09 Oct '15

Dear FILM-Users, Just to raise your awareness about a new imaging facility at Imperial for clinical and pre-clinical imaging that could be complement your imaging at FILM. The Imperial College Biological Imaging Centre (BIC) is a Department of Medicine facility at Hammersmith Hospital Campus. The Centre houses PET/CT, MRI and optical imaging (FMT and IVIS), supporting the scientific community to conduct cutting edge translational research in in vivo disease models. More information at: http://www.imperial.ac.uk/medicine/research-and-impact/facilities/translati… Best wishes, Debora Debora Keller, PhD FILM - Facility for Imaging by Light Microscopy - Facility Manager - Super-Resolution Specialist - Sir Alexander Fleming Building, desk 408 Imperial College London / South Kensington Exhibition Road London SW7 2AZ, UK Phone: +44 (0)207 594 2023 Mobile: + 44(0) 7760 256 889 E-mail: d.keller(a)imperial.ac.uk<mailto:d.keller@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

1 0

[FILM-Users 00589] FW: Free microscopy/spectroscopy conference: Photonex 2015
by Keller, Debora 01 Oct '15

01 Oct '15

FYI: an interesting conference in Coventry 14th/15th October! Dear all, Photonex, our free yearly microscopy/spectroscopy conference (Coventry, Oct the 14th and 15th) is coming soon. This year, we have a great blend of speakers over 4 sessions covering both microscopy and Raman spectroscopy. See below (and attached) the list of talks and speakers. I hope you, or/and your postdocs/students will be able to attend. Please circulate this email to whoever might be interested. Take care, Fred. Prof Steven Bell SERS of Biomaterials: Lessons from DNA Analysis Dr Raphael Levy Gold nanorods as photoacoustic probes to track stem cells in mice Dr Pola Goldberg-Oppenheimer Advanced Hierarchical Micro-platforms for SERS Diagnostics Prof David Richards Multiphoton hyperspectral Raman imaging with CARS Dr Tomas Cizmar Holographic endo-microscopy based on multimode waveguides Dr Johanna Trägårdh Simple excitation sources for non-linear microscopy at long wavelengths Dr Lothar Schermelleh Quantitative 3D super-resolution imaging of functional chromatin topology Prof Rory Duncan Photonic dissection of how neurotransmission works Dr Alexander Corbett Quantitative imaging of intact cardiac tissue using remote focussing microscopy Dr John Day Miniature Fibre Optic Raman Probes: From Cancer to Jet Engines Prof Anthony (Tony) Parker Spatially offset Raman spectroscopy on bone Prof Emmanuel Fort 3D super-resolution microscopy using forbidden light Prof Klaus Suhling Wide-Field Time-Correlated Single Photon Counting Phosphorescence and Fluorescence Lifetime Imaging Dr Achillefs Kapanidis Super-resolution imaging in living bacteria using tracking PALM _________________________________________ Dr. Frederic Festy, Non-clinical lecturer Tissue Engineering and Biophotonics Division King's College London Dental Institute, Room 22, Floor 17, Guy's Tower, Guy's Hospital, London Bridge, London, SE1 9RT, UK, Tel: 020 7188 0143; Fax: 020 7188 182

1 0

[FILM-Users 00588] scratch cleaning TOMORROW
by Keller, Debora 17 Sep '15

17 Sep '15

Dear Users, As you might have noticed, the scratch drive is again fairly full. We will be deleting data older than 2 weeks from scratch at the end of the week. Please make sure you have transferred your data on your own storage space (computer, external HD, server) by Friday 18th noon. As a reminder, scratch is only a transfer server (from the microscope to your own computer) not for storage, data can be deleted at any time. On microscopes, data older than 3 months will be deleted automatically - please remember to keep your data safe on your own storage space (or talk to us regarding OMERO image storage). Best wishes, Debbi Debora Keller, PhD FILM - Facility for Imaging by Light Microscopy - Facility Manager - Super-Resolution Specialist - Sir Alexander Fleming Building, desk 408 Imperial College London / South Kensington Exhibition Road London SW7 2AZ, UK Phone: +44 (0)207 594 2023 Mobile: + 44(0) 7760 256 889 E-mail: d.keller(a)imperial.ac.uk<mailto:d.keller@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

1 0

[FILM-Users 00587] Desk Relocation Hammersmith
by Rothery, Stephen M 16 Sep '15

16 Sep '15

Dear All My desk and the analysis PC in ICTEM have been relocated on the Hammersmith site We have been moved further down the office area - see attached image [cid:image001.jpg@01D0F09B.B505AEB0] Bw Steve FILM - Facility for Imaging by Light Microscopy ************************************************************** Steve Rothery FILM Technician Sir Alexander Fleming Building, desk 403 Imperial College London / South Kensington Exhibition Road London SW7 2AZ Tel +44-(0)207 594 9793 And ICTEM, (L-Block) Room 314 Hammersmith Campus Imperial College London Du Cane Road London, W12 0NN Tel. +44-(0)207 594 1669 E-mail s.rothery(a)imperial.ac.uk<mailto:s.rothery@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility **************************************************************

1 0

[FILM-Users 00586] FW: Save the date - Hounsfield Lecture 2015 - Jürgen Hennig - Magnetic Resonance Imaging: Visions of Life
by Keller, Debora 16 Sep '15

16 Sep '15

See below - some different type of imaging, but should be of interest nonetheless! Cheers Debbi From: Elson, Dan S Sent: 16 September 2015 12:07 To: Keller, Debora Subject: Save the date - Hounsfield Lecture 2015 - Jürgen Hennig - Magnetic Resonance Imaging: Visions of Life Dear Debora Please can you send this email out to your internal email distribution list? It is about a college lecture that will be of interest to your users. Best wishes dan Dear colleagues We are pleased to announce that the Hounsfield Lecture is being re-launched in memory of Nobel Laureate Sir Godfrey Hounsfield. This event brings together medical imaging researchers at ICL and beyond and allows an international leader in biomedical imaging to address the college imaging community. This year's Hounsfield lecture will be given by Professor Jürgen Hennig<https://www.bcf.uni-freiburg.de/people/details/hennig> from the University Medical Center Freiburg. Magnetic Resonance Imaging: Visions of Life Professor Jürgen Hennig Jürgen Hennig is a German chemist and medical physicist, internationally renowned for his work as one of the pioneers of Magnetic Resonance Imaging for clinical diagnostics. He is an alumnus of Imperial and also studied at the universities of Stuttgart, Munich and Freiburg, where he currently works as Scientific Director of the Department of Diagnostic Radiology and Chairman of the Magnetic Resonance Development and Application Center. His awards include the Biosciences and Medicine Max Planck Research Award (2003), the highest scientific award of Taiwan - Tsung Ming Tu Award (2010), and an Einstein professorship from the Chinese Academy of Science (2011). Please save the date, 6 pm Wednesday 4th November, and note the location will be SAF G16. Further details, including information on a medical imaging poster event and competition<http://hamlyn.doc.ic.ac.uk/hounsfield/poster-competition> to be held at 5 pm the same day, can be found on the Hounsfield Lecture website - please submit a poster! http://hamlyn.doc.ic.ac.uk/hounsfield/ Yours sincerely Hounsfield Lecture Organising Committee 2015 Jürgen Hennig - Magnetic Resonance Imaging: Visions of Life Magnetic Resonance Imaging (MRI) has been around for more than 30 years but is still undergoing rapid development. This progress is being driven by innovative technologies as well as new challenges and opportunities arising from the rapid development in biomedical research. MRI not only produces good images, but it also enables non-invasive measurement and visualization of metabolic, microstructural, functional and physiological processes. These offer the potential for studying the multiple parameters associated with normal and disease processes, with applications to areas of medical research including neuroscience, cardiology and metabolic disease. Multiparametric MRI imaging provides an ideal and necessary supplement to genetic profiling and personalized medicine. In conjunction with other imaging techniques, MRI adds a spatiotemporal dimension to the spatially non-specific information provided by genetics. The importance of this can be seen through analysing the genetic heterogeneity of tumors, and MRI allows the detailed study of changes in phenotype caused by genetic modifications in animal models. The richness of phenotype modifications caused by even very isolated genetic changes is ample demonstration that the interrelation of molecular factors with the systemic level is much more than a simple one-to-one correspondence. The clever and creative use of new nano- and microtechnologies has already led to unprecedented improvements in imaging quality and imaging speed as well as new concepts in spatial encoding and the widespread use of high power graphical processor units. The introduction of new technologies is expected to lead to incremental improvements in current approaches to MRI, as well as new applications. Although MRI has come a long way since its early stages, it still has far to go. The Hounsfield Lecture Advances in biomedical imaging technology have allowed tremendous progress in diagnostic and surgical imaging since the first X-ray. Each new development has been based on fundamental scientific progress in science and engineering, resulting in Nobel prizes in medicine for the non-medically trained inventors of techniques such as CT and MRI. Activity that brings together scientists, engineers and clinicians can promote further technological breakthroughs. The Hounsfield Lecture is one such opportunity and allows an international leader in biomedical imaging to address the college imaging community. The event acts as a focus to bring together medical imaging researchers at ICL and beyond and features a poster viewing event and competition that attracts high numbers of submissions from across all faculties. The inaugural event was presented in 2005 and the last was in 2009. Past speakers at the event include James Fujimoto, Rakesh K Jain, Peter Hunter, Sam Gambhir and Robert Balaban.

1 0

[FILM-Users 00585] Travel and Application Fellowships - Symposium on Super-Resolution Microscopy in Infection and Immunity
by Keller, Debora 14 Sep '15

14 Sep '15

Dear Users, See below the announcement for a conference on super-resolution microscopy in Infection & Immunity: Travel fellowships are available so don’t hesitate! Cheers Debbi Dear All, Could you please distribute this information? We're currently organising a Symposium on Super-Resolution Microscopy in Infection and Immunity. It will be Oeiras, Portugal, 21st and 22nd of October 2015. Thanks to the strong support of our sponsors, we have been able to secure a number of fellowships to cover travel and application fees of participants. Priority will be given to PhD students, on a first come first serve basis. For applying please follow the following link http://www.srii.info/registration.html See you in Oeiras on the 21st and 22nd of October. The organising committee, Helena Soares Mariana Pinho Nuno Moreno Ricardo Henriques

1 0