- FILM-Users - imperial.ac.uk

[FILM-Users 00688] !!!!!!!!!!!!!!!!!!!!!!!!!!! DATA DELETION SCRATCH DRIVE !!!!!!!!!!!!!!!!!!!!!!!!!!
by Rothery, Stephen M 06 Oct '16

06 Oct '16

All users The Scratch drive is beginning to fill up again Next Monday we will delete all data from before the 1st July 2016 Please make sure you have copied your data off Best wishes Steve FILM - Facility for Imaging by Light Microscopy ************************************************************** Steve Rothery FILM Technician Sir Alexander Fleming Building, desk 403 Imperial College London / South Kensington Exhibition Road London SW7 2AZ Tel +44-(0)207 594 9793 And ICTEM, (L-Block) Room 314 Hammersmith Campus Imperial College London Du Cane Road London, W12 0NN Tel. +44-(0)207 594 1669 E-mail s.rothery(a)imperial.ac.uk<mailto:s.rothery@imperial.ac.uk> Website: http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ **************************************************************

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[FILM-Users 00687] UPDATE FILM guidelines and policies
by Bruckbauer, Andreas 06 Oct '16

06 Oct '16

Dear FILM users, FILM has recently undergone the annual Health & Safety review and while we have passed with "very good", H&S asked us to clarify some of our policies and you will find them below and in the updated FILM user guide on the internet. Please read these carefully and follow them accordingly. We also put up some new signs regarding waste disposal and the location of the nearest hand washing facilities in the microscope rooms. Furthermore, the cabinet in room 409 (widefield microscopes) is only to be used as a working area and not as a safety cabinet (as it is not certified for such use)! Please make sure that all containers with liquid are labelled with content and your name, even if they contain only distilled water! Lab coats Lab coats must be worn when carrying out any wet lab or in vivo work and when working with organisms requiring higher containment levels; green lab coats are for working with animals. Biological Waste disposal Only class 1 waste can be disposed of in the orange bins provided in the microscope rooms. The appropriate disposal of GM / class 2 and animal waste is the responsibility of the users. GM / class 2 waste must not be disposed in the orange bins unless it has been chemically deactivated or autoclaved. Lone working When using the FILM facility outside the normal hours (07:00 - 19:00 from Monday to Friday) the College's Out of Hours and Lone Working Policies apply and it is the responsibility of the user to get prior authorisation from his or her supervisor and complete a risk assessment. Copies of both, authorisation and risk assessment, have to be shown to FILM staff prior to commencement of the lone working. Best regards Andreas ---------------------------------------------------- Dr Andreas Bruckbauer Academic Visitor Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 401 +44 (0)20 7594 2023 a.bruckbauer(a)imperial.ac.uk<mailto:a.bruckbauer@imperial.ac.uk> ----------------------------------------------------

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[FILM-Users 00686] Welcome Andreas!
by Magee, Tony 05 Oct '16

05 Oct '16

Dear FILM Community, I just wanted to formally welcome Andreas Bruckbauer who has joined us as maternity cover for Debbi and will be managing FILM. Andreas will be glad to meet as many of you as possible over the coming weeks and months. Particularly, at the moment we are preparing an application to The Wellcome Trust for a Zeiss 880 with Airyscan to replace our ageing and no-longer-supported LSM510 and Andreas would love to hear from anyone who has potential applications for the 880/Airyscan. By the way, for those of you who don't know, Debbi had a lovely little girl Katja who is happy and healthy. Cheers, Tony Anthony I. Magee Academic Lead of FILM Professor of Membrane Biology Head of Section of Molecular Medicine National Heart and Lung Institute Imperial College London Sir Alexander Fleming Building, room 106 South Kensington Campus London SW7 2AZ UK Tel. +44 (0)207 594 3135 [Athena Swan logo]

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[FILM-Users 00685] FIJI Workshop in Leicester
by Gaboriau, David C A 05 Oct '16

05 Oct '16

Dear Users, Please see below for a ImageJ/FIJI workshop in Leicester. Best wishes, David ---------------------------------------------------------------------------------------------------------- Dear List members, I will organize the next ImageJ/Fiji workshops in Leicester on Monday 12 and Tuesday 13 December 2016. The workshops are a mixture of lectures and hands-on sessions. No prior knowledge of ImageJ, Fiji or macro coding is required, only basic use of a computer. For further information and registration please visithttp://www2.le.ac.uk/colleges/medbiopsych/facilities-and-services/cbs/lite/aif/workshops/imagej-workshops-December-2016. Best wishes Kees Dr Ir K.R. Straatman Senior Experimental Officer Centre for Core Biotechnology Services University of Leicester http://www2.le.ac.uk/colleges/medbiopsych/facilities-and-services/cbs/lite/… ---------------------------------------------------------------------------------------------------------- David Gaboriau, PhD FILM - Facility for Imaging by Light Microscopy High-Content Microscopy Specialist Sir Alexander Fleming Building, desk 408 Imperial College London / South Kensington Exhibition Road London SW7 2AZ, UK Phone: +44 (0)2075 949 793 Email: d.gaboriau(a)imperial.ac.uk<mailto:d.gaboriau@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

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[FILM-Users 00684] Post doc in Paris: biosensors for ROS detection
by Gaboriau, David C A 28 Sep '16

28 Sep '16

Dear Users, Please see below a PostDoc offer in Paris. All the best, David -----Original Message----- From: gdr2588-request(a)services.cnrs.fr [mailto:gdr2588-request@services.cnrs.fr] On Behalf Of heliot Sent: 28 September 2016 14:49 To: gdr2588(a)services.cnrs.fr Subject: [GDR2588-MIV] Post doc: biosensors for ROS detection Bonjour, Pourriez vous faire suivre cette annonce pour un post doc au LCP à Orsay? Merci beaucoup d'avance, Marie -- Marie Erard Université Paris Sud Laboratoire de Chimie Physique Bat 350 pièce 163 91405 Orsay Cedex Tel: 01 69 15 30 14 ou 30 31 Email: marie.erard(a)u-psud.fr

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[FILM-Users 00683] FOCUS ON MICROSCOPY 2017
by Bruckbauer, Andreas 27 Sep '16

27 Sep '16

This is a great conference about everything related to (light) microscopy: Best wishes Andreas ----------------------------------- Announcing: FOCUS ON MICROSCOPY 2017 Bordeaux, France. April 9-12, 2017 30h International Conference on 3D Image Processing in Microscopy 29th International Conference on Confocal Microscopy Dear Colleagues, After the successful FOM2016 conference held in Taipei earlier this year, it is a pleasure to announce FOM2017: the next in a series of unique interdisciplinary meetings on advanced multi-dimensional light microscopy and image processing. The conference will take place at the Palais des Congrès de Bordeaux, Convention Center, Bordeaux, France. For further details please visit the FOCUS ON MICROSCOPY website http://www.FocusOnMicroscopy.org<http://www.focusonmicroscopy.org/>. There, in addition to general information, the scientific program of previous FOM conferences - together with PDF's of presented abstracts- can be found under the link "Past conferences" Please note that the Sunday program is different from previous years. The (free) tutorials start at 10:30. After lunch regular parallel sessions begin at 14:00 hours followed by a Flash poster presentation session at 16:00. The Plenary opening session will start around 17:30 followed by a Welcome reception. For details see the tentative program on the website. FOM2017 in Bordeaux is the continuation of a yearly conference series (since 1988) on the latest innovations and developments in mostly optical microscopy and their application in biology, medicine, and materials sciences. Key topics are: - Theory and practice of confocal and multiphoton-excitation microscopy - Super-resolution, nanoscopy imaging: from PSF engineering (4pi, SIM, STED), fluorescent activation/quenching, stochastic/centroid (PALM, STORM, GSDIM, SOFI and related techniques) to TIRF - 3D and 4D live cell and tissue imaging - Adaptive optics for microscopy - Developments in phase/interference microscopies - Light sheet microscopy - Advanced fluorescence imaging/spectroscopy: FRET, FRAP, FLIM, FCS,SOFI - New fluorescence probes, proteins, quantum dots, single molecule imaging - Clearing and expansion techniques. - Coherent non-linear microscopies: SHG, THG, SFG, CARS. - Developments in phase/interference microscopies - Multi-dimensional fluorescence and Raman spectroscopy imaging - Correlated light/electron microscopy - Laser manipulation and tracking, photo-activation - OCT, endoscopy - Fast acquisition, automated and high-content microscopy - 3D image processing and visualization for multidimensional data At the 2017 conference the topic "Single object - molecule, Quantum Dot, Nanotube - based imaging will receive special attention. The conference series is in addition known for covering the rapid development of advanced fluorescence labeling techniques for (super)- resolution and multi-photon 3D imaging of -live- biological specimens. Abstracts for contributions are invited and can already be submitted through the website: http://www.FocusOnMicroscopy.org <http://www.focusonmicroscopy.org/> where further information on the present and previous FOM conferences can be found. Important dates: Deadline for the submission of abstracts January 17, 2017 Acceptance of abstracts, program on the web February 10, 2017 Deadline for early registration March 2, 2017 FOM2017 Conference April 9 - 12, 2017 Easter Sunday 2017 April 16, 2017 To stay informed about the conference please leave your name and email at http://www.FocusOnMicroscopy.org/stayinformed<http://www.focusonmicroscopy.org/stayinformed> Welcoming you to Bordeaux for the FOM2017 conference and exhibition, the FOM2017 organizers: * Lionel Canioni, University Bordeaux, France * Fred Brakenhoff, University of Amsterdam, The Netherlands E-mail: info2017(a)FocusOnMicroscopy.org<mailto:info2017@FocusOnMicroscopy.org> Web: http://www.FocusOnMicroscopy.org<http://www.focusonmicroscopy.org/>

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[FILM-Users 00682] Sharepoint is accessible again
by Gaboriau, David C A 23 Sep '16

23 Sep '16

Dear Users, Sharepoint is accessible again, sorry for the inconvenience. Best wishes David David Gaboriau, PhD FILM - Facility for Imaging by Light Microscopy High-Content Microscopy Specialist Sir Alexander Fleming Building, desk 408 Imperial College London / South Kensington Exhibition Road London SW7 2AZ, UK Phone: +44 (0)2075 949 793 Email: d.gaboriau(a)imperial.ac.uk<mailto:d.gaboriau@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

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[FILM-Users 00681] Temporal SharePoint outage
by Bruckbauer, Andreas 23 Sep '16

23 Sep '16

Dear all, Our microscope booking pages are currently unavailable due to a SharePoint outage, I will keep you updated. Best wishes Andreas Sent from my Phone ________________________________ From: Hill, James<mailto:j.hill@imperial.ac.uk> Sent: ‎23/‎09/‎2016 11:02 To: Bruckbauer, Andreas<mailto:a.bruckbauer@imperial.ac.uk> Subject: RE: SharePoint down? Yes it is, Issues with the ESX farm, I’ll let you know when I get updated re availability. Regards James From: Bruckbauer, Andreas Sent: 23 September 2016 11:00 To: Hill, James <j.hill(a)imperial.ac.uk> Subject: SharePoint down? Is SharePoint down? Our microscope booking system runs on SharePoint. Best wishes Andreas Sent from my Phone

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[FILM-Users 00680] Nikon Workshop at FILM
by Gaboriau, David C A 21 Sep '16

21 Sep '16

Dear Users, We now have a more detailed programme of the workshop with Nikon at the end of November. If you would like to book a session on either the iSIM or the High Content system, please fill in the attached questionnaire and send it to Emily or Natalia at Nikon (their emails are in the questionnaire). -------------------------------------------------------------------------------------------------------------------------------------------- Nikon will be holding a five day workshop at FILM, Imperial College London on 24th – 30th November. The workshop will include: · Demonstrations of the iSIM - a new array-scanning confocal system from VisiTech International, enabling live cell imaging at super-resolution. · Showcase of the new High Content System, Nikon Eclipse Ti-E with C2 Confocal, located in the FILM– sessions focusing on the use of JOBS software · Seminar on the iSIM and Nikon high content software JOBS Please see the attached flyers for more information and details on how to book your workshop session with a Nikon Imaging Specialist. If you wish to book a hands-on session on either system, please complete the attached questionnaire and Nikon will contact you directly to make the arrangements. -------------------------------------------------------------------------------------------------------------------------------------------- All the best, David David Gaboriau, PhD FILM – Facility for Imaging by Light Microscopy High-Content Microscopy Specialist Sir Alexander Fleming Building, desk 408 Imperial College London / South Kensington Exhibition Road London SW7 2AZ, UK Phone: +44 (0)2075 949 793 Email: d.gaboriau(a)imperial.ac.uk<mailto:d.gaboriau@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

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[FILM-Users 00679] Grant application for LSM880 confocal with Airyscan
by Bruckbauer, Andreas 19 Sep '16

19 Sep '16

Dear all, I am very excited to start as maternity cover for Debora Keller in FILM from October and already spend some time here at SAF to settle in. With Tony we agreed that we should re-apply for the LSM880 with Airyscan in the next WT MUEG round. This would take some strain off the aging LSM510 at South Kensington and add functionality to our range of confocal microscopes. Please contact me if you would like to be considered as a coapplicant and could, in due course, provide a paragraph supporting the 880/Airyscan purchase, ideally with supporting preliminary data. Best wishes Andreas ---------------------------------------------------- Dr Andreas Bruckbauer Academic Visitor Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 401 +44 (0)20 7594 2023 a.bruckbauer(a)imperial.ac.uk<mailto:a.bruckbauer@imperial.ac.uk> ----------------------------------------------------

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