- FILM-Users - imperial.ac.uk

[FILM-Users 00699] Fast microfluidic temperature control for high resolution live cell imaging
by Bruckbauer, Andreas 11 Jan '17

11 Jan '17

FYI, from Alex Cerise http://www.cherrybiotech.com/ In collaboration with the Institut Curie and the ENS Paris, we developed a system allowing an 0.1°C precise and stable temperature control on a large 5 to 45°C range. Publication : Guilhem Velve Casquillas et al. Lab Chip, 2011, 11, 484-489 Abstract: One major advantage of using genetically tractable model organisms such as the fission yeast Schizosaccharomyces pombe is the ability to construct temperature-sensitive mutations in a gene. The resulting gene product or protein behaves as wildtype at permissive temperatures. At non-permissive or restrictive temperatures the protein becomes unstable and some or all of its functions are abrogated. The protein regains its function when returning to a permissive temperature. In principle, temperature sensitive mutation enables precise temporal control of protein activity when coupled to a fast temperature controller. Current commercial temperature control devices do not have fast switching capability over a wide range of temperatures, making repeated temperature changes impossible or impractical at the cellular timescale of seconds or minutes. Microfabrication using soft-lithography is emerging as a powerful tool for cell biological research. We present here a simple disposable polydimethylsiloxane (PDMS) based microfluidic device capable of reversibly switching between 5 _C and 45 _C in less than 10 s. This device allows high-resolution live cell imaging with an oil immersion objective lens. We demonstrate the utility of this device for studying microtubule dynamics throughout the cell cycle.

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[FILM-Users 00696] Airyscan demo at Imperial January 23-27
by Bruckbauer, Andreas 16 Dec '16

16 Dec '16

Dear FILM users, We will have a one-week demo of the new Zeiss LSM 880 with fast Airyscan mode here at Imperial. This is a fantastic opportunity to thoroughly test this microscope with your samples under real conditions for live imaging and other. We are currently applying for funding for a new confocal microscope and this would be one of the options. This instrument should have better sensitivity and resolution than our current confocal microscopes. The demo will be in the week January 23-27, so pretty soon after the holidays and we need to plan a schedule for the experiments. Please let me, Steve or David know if you want to take part in this and what you like to image. Zeiss will do a presentation about the system on January 19th at 12:00 -13:00 here in the SAF building Here is the summary of the grant application with a bit more detail: Confocal imaging accounts for a large fraction of the usage in the FILM facility including live imaging, however the current instruments have their limitations in sensitivity and achievable resolution. We request a latest generation, highly sensitive confocal microscope (Zeiss LSM880 Airyscan or Leica SP8 HyVolution) for high resolution live imaging. This will allow the use of much lower laser power than conventional confocal microscopes enabling the users to image live samples with decreased phototoxicity for longer. The increase in sensitivity is not only due to modern detector technology such as GaAsP or HyD detectors, but also new imaging modes like the Zeiss Airyscan, which allows high resolution imaging with better light throughput than conventional confocal imaging. The Zeiss Airyscan also supports a new fast mode with 4-times faster image acquisition, whereas the Leica system has a resonance scanner for fast scanning. Both systems have ~1.7-fold improvement in resolution compared to normal confocal imaging, enabling the distinction of smaller features within cells without special sample preparation steps. Because new imaging technologies generate much more data, we apply for an upgrade of the existing network infrastructure linked to a new data storage server and a powerful analysis. Best wishes Andreas ---------------------------------------------------- Dr Andreas Bruckbauer FILM facility manager Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 401 +44 (0)20 7594 2023 a.bruckbauer(a)imperial.ac.uk<mailto:a.bruckbauer@imperial.ac.uk> http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ ----------------------------------------------------

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[FILM-Users 00697] !!!!!!!!!!!!! Scratch Data Deletion !!!!!!!!!!!!!!!!!!!!!!
by Rothery, Stephen M 16 Dec '16

16 Dec '16

Dear All Scratch is getting full again and we need to delete older data so next week we will remove all data from before 1st August 2016 Please make sure you have backed up your data Also as a reminder - Scratch is for data transfer only and not for storage or for your own personal files Many thanks Steve FILM - Facility for Imaging by Light Microscopy ************************************************************** Steve Rothery FILM Technician Sir Alexander Fleming Building, desk 403 Imperial College London / South Kensington Exhibition Road London SW7 2AZ Tel +44-(0)207 594 9793 And ICTEM, (L-Block) Room 314 Hammersmith Campus Imperial College London Du Cane Road London, W12 0NN Tel. +44-(0)207 594 1669 E-mail s.rothery(a)imperial.ac.uk<mailto:s.rothery@imperial.ac.uk> Website: http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ **************************************************************

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[FILM-Users 00695] NEUBIAS Short Term Scientific Missions
by Gaboriau, David C A 16 Nov '16

16 Nov '16

Dear Users, Please see below for opportunities of mobility grants in image analysis from NEUBIAS. Deadline for applications is 30th November. All the best, David Dear all, NEUBIAS, the network of European BioImage Analysts funded by the EU framework COST, has opened a new activity that will run continuously for 4 years, and is centered on mobility grants, also called Short-Term Scientific Missions. In a Nutshell, BioImage Analysts and Life Scientists, from Research Labs AND Core facilities, can apply for funds to cover their expenses when visiting a Host-Lab in a different country (Lab or industry) where they will perform a short scientific project strictly focused on BioImage Analysis, and that should enable: 1) collaborations on innovative image analysis methods, 2) access to big data analysis technology and/or image analysis tools for scientists lacking them locally, 3) knowledge transfers to support careers and regional development. Please check all the information here: http://eubias.org/NEUBIAS/?page_id=707 and contact these people for any question: Julia Fernandez Rodriguez - NEUBIAS STSM Coordinator - juliafer(a)cci.sahlgrenska.gu.se<mailto:juliafer@cci.sahlgrenska.gu.se> Clara Prats - NEUBIAS WG7 Co-Leader - cprats(a)sund.ku.dk<mailto:cprats@sund.ku.dk> Julien Colombelli - NEUBIAS Action Chair - julien.colombelli(a)irbbarcelona.org<mailto:julien.colombelli@irbbarcelona.org> Best greetings Julia, Clara and Julien on behalf of NEUBIAS members STSM grants - NEUBIAS: Network of BioImage Analysts<http://eubias.org/NEUBIAS/?page_id=707> [https://i.embed.ly/1/image?url=http://eubias.org/NEUBIAS/wp-content/uploads…] David Gaboriau, PhD FILM - Facility for Imaging by Light Microscopy High-Content Microscopy Specialist Sir Alexander Fleming Building, desk 408 Imperial College London / South Kensington Exhibition Road London SW7 2AZ, UK Phone: +44 (0)2075 949 793 Email: d.gaboriau(a)imperial.ac.uk<mailto:d.gaboriau@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

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[FILM-Users 00694] Fw: Last chance to fill out: NEUBIAS Questionnaire (UK): do you use or develop bioimage analysis for life sciences research?
by Gaboriau, David C A 15 Nov '16

15 Nov '16

Dear Users, Please see below a link to a survey on the use of image analysis in the life sciences, on behalf of NEUBIAS, the Network of European Image Analysts. If you use or develop image analysis tools in your research, we would like to hear from you! All the best, David ---------- Forwarded message ---------- From: Dominic Waithe <dominic.waithe(a)imm.ox.ac.uk<mailto:dominic.waithe@imm.ox.ac.uk>> Date: Fri, Nov 11, 2016 at 4:09 PM Subject: Last chance to fill out: NEUBIAS Questionnaire (UK): do you use or develop bioimage analysis for life sciences research? To: CONFOCALMICROSCOPY(a)lists.umn.edu<mailto:CONFOCALMICROSCOPY@lists.umn.edu> ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Dear mailing list users, We have had some great input for our questionnaire, so thank you to those who have filled it out. We are just trying to catch any last image analysts whom we may have missed on our first pass. If you are working in the UK and you do some kind of image analysis in your work and you haven't filled out the questionnaire below then we would would be very grateful if you could read the below message and complete the linked questionnaire. NEUBIAS (Network of European BioImage Analysts) is a new initiative designed to link and develop BioImage Analyst training and careers, and to improve and further extend image analysis techniques within the life sciences: http://www.cost.eu/COST_Actions/ca/CA15124 http://www.eubias.org/NEUBIAS/ We are emailing as the UK representatives in the NEUBIAS Management Committee. We are compiling a list of UK academics in, or associated with, the life sciences who are are involved in developing bioimage analysis tools and/or benefit from some kind of bioimage analysis in their work. This list will help us to define the extent of the UK bioimage analysis community, and to co-ordinate UK NEUBIAS activities. For example, there will be plenty of opportunities for individuals to be involved through joining workgroups (contributing to the deliverables of the scheme), or attending workshops, conferences and taggathons, or hosting bioimage analysts within labs for knowledge exchange purposes and/or short projects. Although this questionnaire is only for UK members right now we expect it to be closely replicated by other member countries shortly. We want to hear from you if you are one of the following: - A scientist who applies image analysis tools in their work, but does not necessarily develop new image analysis techniques. - A researcher who develops image analysis techniques, but for whom it is not the main focus of their work. - An individual who directly develops or researches image analysis techniques within the life sciences (e.g. Image analysts, or quantitative bioimage analysts). - A computer vision scientist, mathematician or physicist, or other specialist who is interested in, or actively participating in, research related to image analysis being applied within the life sciences. Please visit and fill out our very short questionnaire form at: https://www.surveymonkey.co.uk/r/FGCBFRY Best wishes, Dominic Waithe, Graeme Ball, Lucy Collinson, Martin Jones. UK members of the NEUBIAS Management Committee

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[FILM-Users 00693] STORM workshop & "Imagine-Imaging Life Symposium" in Sheffield, January 2017 - REGISTRATION OPEN
by Keller, Debora 09 Nov '16

09 Nov '16

Dear all, There will be 2 events of interest for the imaging community and students in january 2017 in Sheffield: 1) "Imagine - Imaging Life launch symposium<http://www.imagine-imaginglife.com/launch-symposium.html>" on 12-13th of january with a great line-up of speakers on the topic of combining different cutting-edge imaging technologies (super-resolution fluorescence microscopy, electron microscopy and atomic force microscopy...) to address important biological and biomedical problems. see attached flyer 2) ahead of the symposium, on the 11th of january, there is going to be a STORM workshop: https://sheffieldstorm.wordpress.com/ this workshop will focus on more practical aspects of STORM imaging (sample prep, buffers, analysis, etc) and users are encouraged to bring their images and questions. Even I was tempted out of mat-leave for a day to attend and give a talk :-) See attached flyer too - questions to: c.walther(a)sheffield.ac.uk<mailto:c.walther@sheffield.ac.uk> Both events would be of interest to FILM-users and the wider london super-res crew! Cheers from the snowy/sleety Sheffield Debbi Debora Keller, PhD FILM - Facility for Imaging by Light Microscopy - Facility Manager - Super-Resolution Specialist - Sir Alexander Fleming Building, desk 408 Imperial College London / South Kensington Exhibition Road London SW7 2AZ, UK Phone: +44 (0)207 594 2023 Mobile: + 44(0) 7760 256 889 E-mail: d.keller(a)imperial.ac.uk<https://exchange.imperial.ac.uk/owa/redir.aspx?C=ly2HSraUakenznQlUce8xI9f3N…> Website: http://imperial.ac.uk/imagingfacility<https://exchange.imperial.ac.uk/owa/redir.aspx?C=ly2HSraUakenznQlUce8xI9f3N…>

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[FILM-Users 00692] Fw: Nikon Workshop at FILM
by Gaboriau, David C A 26 Oct '16

26 Oct '16

Dear Users, Just a reminder that we will host a five day workshop with Nikon, with a showcase on the High-Content system and a demo of the iSIM live-cell super-resolution system. There are still places left to book sessions with Nikon Imaging Specialists on both microscopes, please fill in the attached questionnaire and send to Emily or Natalia at Nikon. Nikon will be holding a five day workshop at FILM, Imperial College London on 24th - 30th November. The workshop will include: · Demonstrations of the iSIM - a new array-scanning confocal system from VisiTech International, enabling live cell imaging at super-resolution. · Showcase of the new High Content System, Nikon Eclipse Ti-E with C2 Confocal, located in the FILM- sessions focusing on the use of JOBS software · Seminar on the iSIM and Nikon high content software JOBS Please see the attached flyers for more information and details on how to book your workshop session with a Nikon Imaging Specialist. If you wish to book a hands-on session on either system, please complete the attached questionnaire and Nikon will contact you directly to make the arrangements. All the best, David David Gaboriau, PhD FILM - Facility for Imaging by Light Microscopy High-Content Microscopy Specialist Sir Alexander Fleming Building, desk 408 Imperial College London / South Kensington Exhibition Road London SW7 2AZ, UK Phone: +44 (0)2075 949 793 Email: d.gaboriau(a)imperial.ac.uk<mailto:d.gaboriau@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

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[FILM-Users 00691] YLS 2016 Symposium: Advanced Imaging with Light Microscopy
by Gaboriau, David C A 17 Oct '16

17 Oct '16

Dear Users, Just to remind you of the upcoming Young Life Scientists' symposium on Friday 28th October. There are about 10 places left for this exciting event! Please see below to register. There is also an image competition: Hope to see you there! All the best, David Dear YLS 2016 attendees, We are writing to remind you of the upcoming YLS 2016 Symposium "Advanced Imaging with Light Microscopy: Capturing Tissues to Single Molecules". We still have some remaining places for abstract submissions for either a poster or oral presentation, with amazing prizes for the winners. We strongly encourage as many people as possible to submit abstracts before the deadline on Friday 7th October, so why not give it a go? We would also like to encourage you to submit light microscopy images for the image competition. Zeiss have very kindly offered an incredible prize for the best image and in the field of light microscopy, seeing IS believing! Please provide a maximum of 2 images in high enough resolution to be printed at size A4 in either .TIF or .png formats. Presenting a poster, giving an oral presentation or displaying an image provides the perfect way to initiate discussions and foster new collaborations. Please send all abstract submissions and light microscopy images directly to yls2016symposia(a)gmail.com<mailto:yls2016symposia@gmail.com>. Kind regards, The YLS 2016 team Stephanie, Mark, Silvia & Katie ----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- Dear colleague, We would like to invite you to the Young Life Scientists' Symposium 2016 "Advanced Imaging with Light Microscopy: Capturing Tissues to Single Molecules", which will take place on Friday 28th October 2016 at Imperial College London, Hammersmith Hospital Campus, W12 0NN. The YLS Symposium is a free event open to everyone who is using microscopy techniques for their research and wants to gain a deeper understanding in this field. Focusing on application of emerging advanced imaging techniques, image analysis and current research into imaging complex cellular processes, the YLS Symposium 2016 will cover a wide range of techniques and their applications, together with sample preparation and post-acquisition analysis workshops. Keynote speakers feature Prof. Francesco Pavone, Laurent Cognet and Lothar Schermelleh, who will present their cutting edge research and major achievements during plenary lectures, followed by a Q&A session. The programme also includes oral presentations selected from abstracts, a poster session, an image competition (prizes would be given) and a discussion panel. The Symposium will be the perfect occasion for networking over provided coffee-breaks, lunch and a drinks reception. For more information, registration and abstract submission please visit the YLS Symposium 2016 Eventbrite page: https://www.eventbrite.co.uk/e/young-life-scientists-symposium-2016-tickets… Please note that number of places is limited! Please do not hesitate to forward this email to whoever might be interested in participating in this event. Kind regards, The YLS Symposium 2016 organizers, Katie, Mark, Silvia and Stephanie

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[FILM-Users 00690] FW: [lsr-group] Advanced Cell Biology/Cytoskeletal job at Imperial College London
by Gaboriau, David C A 13 Oct '16

13 Oct '16

Dear Users, Please find attached the advert for a post-doc in super-resolution microscopy at Imperial. Best wishes, David From: lsr-group-bounces(a)imperial.ac.uk [mailto:lsr-group-bounces@imperial.ac.uk] On Behalf Of Owen, Dylan Sent: 13 October 2016 11:29 To: lsr-group <lsr-group(a)imperial.ac.uk> Subject: [lsr-group] Advanced Cell Biology/Cytoskeletal job at Imperial College London Super-res post-doc job available in London, see attavhed From: Baum, Jacob [mailto:jake.baum@imperial.ac.uk] Sent: 13 October 2016 07:37 To: Owen, Dylan Subject: Advanced Cell Biology/Cytoskeletal job at Imperial College London Dear Dylan I wondered if you could send the following job advert round the LSR membership for a postdoc position at Imperial. With very best wishes Jake

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[FILM-Users 00689] JPK optical tweezers
by Bruckbauer, Andreas 06 Oct '16

06 Oct '16

A colleague from the Crick has an optical tweezers system on offer for a good price, please see below Best wishes Andreas ---------------------------------------------------- From: Nicholas Cade [mailto:Nicholas.Cade@crick.ac.uk] Sent: 06 October 2016 11:17 To: Bruckbauer, Andreas <a.bruckbauer(a)imperial.ac.uk> Subject: JPK optical tweezers Unfortunately we won't be able to take our optical trap with us when we move into the new building, and I've been trying to find a new home for it. None of the other groups joining us will have the space for it. Do you know anybody at Imperial that might be interested? I've put the details below and attached the original quotation showing the system specifications - please feel free to forward this email. I'm told we only need to recover the depreciated value (~£25k). http://www.jpk.com/sid9a4635252f2abd32eabb745c5046bce7/nanotracker-2-overvi… Some of the key features are: · 3W 1064nm trapping laser · Two independent trapping beams with full range of power splitting between the two. · One beam can be split into additional multiplexed traps using an AOD · Three dimensional trap position control, independent of observation plane · Two independent detection modules for each beam, each with separate QPD (xy) and photodiode (z) · Full access to all signals for further external control / monitoring Fully software controlled experiments for · Automatic calibration of trap stiffness · Force spectroscopy (particle moving in a fixed trap) · Force-feedback particle tracking at arbitrary angles We are under some time pressure to decide on the fate of the system as we move at the end of October and obviously if anyone wanted to see the system working it would need to be done before then. Many thanks Nic ________________________________________________________ Dr Nic Cade Principal Laboratory Research Scientist Microtubule Cytoskeleton Laboratory The Francis Crick Institute Lincoln's Inn Fields Laboratory 44 Lincoln's Inn Fields London WC2A 3LY T: +44 20 7269 3383 E: Nicholas.cade(a)crick.ac.uk<mailto:Nicholas.cade@crick.ac.uk> W: www.crick.ac.uk<http://www.crick.ac.uk/> The Francis Crick Institute Limited is a registered charity in England and Wales no. 1140062 and a company registered in England and Wales no. 06885462, with its registered office at 215 Euston Road, London NW1 2BE.

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