- FILM-Users - imperial.ac.uk

[FILM-Users 00739] !!! Student presentation prize for FILM microscopy day 2017
by Bruckbauer, Andreas 13 Jun '17

13 Jun '17

Dear all, This year we will have a prize for the best Match-maker student presentation at the microscopy day :) Please discuss with your supervisor and contact me if you wish to present a 5 mins snapshot of your work! !!! The FILM microscopy day 2017 will be held on Tuesday June 27th Keynote presentation by Michael Way from The Francis Crick Institute, please see the preliminary program below. This year's event will be sponsored by: Hamamatsu, Leica, Nikon, Stratech and Zeiss Looking forward to seeing you in two weeks! Best wishes Andreas Preliminary Program: 9:30 - 9:45 Tony Magee: Welcome & latest FILM news 9:45 - 10:30 Andreas Bruckbauer: From microscopy basics to state of the art applications . 10:30 - 11:00 Stephen Rothery: Data Analysis in Fiji. 11:00-11:30 coffee & tea break 11:30 - 12:00 Debora Keller: PPMS, our new online booking system. 12:00 - 12:30 Microscopy Match-Maker: FILM users present their research performed in FILM through 5 min snapshot presentations. 12:30 - 13:30 sandwich lunch 13:30 - 14:15 David Gaboriau: High Content screening 14:15 - 14:45 Technobytes - 10min presentation from our sponsors: Paul McCormick, Leica microsystems, Full Spectral Freedom for Multi-Colour, Deep Imaging experiments with SP8 DIVE' Gareth Bolitho, Stratech Scientific Ltd, TBC 14:45 - 15:30 Cristina Lo Celso, Imperial College London: Imaging healthy and malignant haematopoiesis: active cells in a dynamic environment. 15.30 - 16.00 coffee & tea break Plenary Lecture 16:00 - 16:55 Michael Way, The Francis Crick Institute: Using Vaccinia virus to understand signalling and cytoskeletal dynamics. 16:55 - 17:00 Tony Magee: Round-up. 17:00 - 17:30 The plenary lecture will be followed by drinks and nibbles ---------------------------------------------------- Dr Andreas Bruckbauer FILM facility manager Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 401 +44 (0)20 7594 2023 a.bruckbauer(a)imperial.ac.uk<mailto:a.bruckbauer@imperial.ac.uk> http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ ----------------------------------------------------

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[FILM-Users 00738] BioImage Analysis training school - deadline extended to 02 June
by Gaboriau, David C A 31 May '17

31 May '17

Dear Users, The deadline to apply for the NEUBIAS BioImage Analysis training school for Early Career researchers (PhD Students and Post Docs) has been extended to the 2nd June. Please see below for details. All the best, David David Gaboriau, PhD FILM - Facility for Imaging by Light Microscopy High-Content Microscopy Specialist Sir Alexander Fleming Building, desk 408 Imperial College London / South Kensington Exhibition Road London SW7 2AZ, UK Phone: +44 (0)2075 949 793 Email: d.gaboriau(a)imperial.ac.uk<mailto:d.gaboriau@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility ------------------------------------------------- Dear colleagues, the deadline for applications for the NEUBIAS "early career" and "staff" training schools has been extended to June 2nd! Below follows the initial announcement ========== NEUBIAS, the Network of European BioImage Analysts (www.neubias.org<http://www.neubias.org/>), is delighted to announce two new Training Schools on BioImage Analysis: Early Career Investigators (life scientists) Facility staff (imaging & analysis staff) The focus of training will be on construction and automation of Image Analysis workflows, using as examples more than one toolbox and different exercises. The schools will be held in Gothenburg 11-14th of September 2017, hosted by the Centre for Cellular Imaging - Sahlgrenska Academy, University of Gothenburg, Sweden. NEUBIAS schools are an excellent opportunity to learn from many experts in bioimage analysis (we are expecting ~40 specialists at the event) and "....a great mix of intensive learning and community networking" (former trainee testimonial!). - applications for Gothenburg are open (each school has 25 available seats and 10-12 trainers). - Within the COST framework, a few travel grants are offered to applicants who qualify. - NEW Registration deadline: June 2nd, 2017 (must include "letter of motivation"). - Selection notification: 2nd week of June 2017. More information about schools (programme & trainers) and venue, travel & lodge available at our website: www.neubias.org<http://www.neubias.org/> -> "Training Schools" http://eubias.org/NEUBIAS/training-schools/eci/ts4-gothenburg2017/ http://eubias.org/NEUBIAS/training-schools/staff/ts5-gothenburg2017/ We kindly ask that you help us reach all potential interested applicants. On behalf of all NEUBIAS members, Julien Colombelli, Chair; Kota Miura, Vice-Chair Julia Fernandez-Rodriguez, Local organizer Carolina Wählby, Jan Eglinger, Joakim Lindblad & Nuno P Martins, TS4&5 programme organizers Gaby G Martins & Fabrice Cordelières, WG2-Training leaders Perrine Paul-Gilloteaux, WG4-Webtool leader Sébastien Tosi, WG5-Benchmarking & Sample Datasets leader --------------------------------------------------------------- NEUBIAS is an European network of currently ~180 members and 35 countries, which aims to promote the communication between Life Scientists, Instrumentalists, Developers and BioImage Analysts and to establish and promote the role of Bioimage Analysts in Life Science. Our mission includes: - A training programme for 3 different target audiences:Early Career researcher, Facility Staff, Analyst (running until 2020 - expected 400 trainees and 15 training schools). - Promote different yearly events (NEUNIAS2020 Conference, workshops [training schools], Taggathons) - Online Resources: Repository of tools and workflows, Benchmarking and Sample datasets, Training material and Open Textbook. - A Short Term Scientific Mission mobility programme for Scientists to visit Host Labs and get in depth insights into cutting edge Image Analysis technology. - Outreach material. ---------------------------------------------------------------------

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[FILM-Users 00737] FW: Seminar by Alex Rohrbach
by Bruckbauer, Andreas 30 May '17

30 May '17

FYI From: Dunsby, Christopher W Sent: 30 May 2017 16:47 To: Bruckbauer, Andreas <a.bruckbauer(a)imperial.ac.uk> Subject: Seminar by Alex Rohrbach Dear Andreas, The Photonics seminar this week by Alex Rohrbach on light sheet microscopy and optical tweezers may be of interest to some FILM users. Please could you advertise this via the FILM mailing list? best regards, Chris Professor Alexander Rohrbach http://www.imtek.de/professuren/bnp/startseite_bio-und_nanophotonik Thursday 1st June 4-5pm Huxley room 711c Novel photonics to observe biophysics on the small and fast scale Many new, exciting imaging techniques have emerged during the last decade, providing significantly improved spatial resolution and contrast. However, this extra information comes at the cost of more photons required to illuminate the target, which requires more time and energy and often damages biological structures. The smaller the structures to be investigated, the faster they usually move inside living cells, because of both Brownian motion and coordinated work of molecular motors. Can scattered laser light be a solution to the problem? In addition, a large fraction of fluorescence photons is scattered too often inside large objects and therefore diffusive photons do not reach the right pixels on the camera. A separation of diffusive photons would be helpful. In this talk I will demonstrate how nanometer scale displacements and motions of cellular structures and artificial probes can be observed by coherent optics, giving novel insights into principles of molecular mechanics of living systems. In addition, I will take a second look with you at the spreading of diffusive photons and how to separate their negative influence on image quality.

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[FILM-Users 00736] probes for multiphoton microscopy
by Bruckbauer, Andreas 30 May '17

30 May '17

FYI, probes for multiphoton microscopy from Stratech, please contact Gareth for a brochure Andreas ------------------------ The current probes are for; b-Amyloid Hydrogen Peroxide Mitochondrial Thiol b-Galactosidase Lipid Rafts Nitric Oxide Calcium Ion Lysozomes Sodium Ion Glucose Mitochondrial Hydrogen Sulfide Zinc Ion These probes have been developed specifically for two photon microscopy and I hope that the probes offered will be useful in the work that you are doing. We would be more than happy to discuss/present them further with you and your users when convenient for you. We will also be more than happy to arrange samples for you to testing. We would also be very grateful for any feedback that you may have feedback regarding the range and future probes that would be useful going forwards. I look forward to hearing from you. Best wishes, Gareth. Gareth Bolitho Sales Director www.Stratech.co.uk<http://www.stratech.co.uk/> Stratech Scientific Ltd. Unit 7, Acorn Business Centre Tel: +44 (0)1638 782600 Mob: +44 (0)7889 721351 Newmarket, Suffolk. CB8 7SY Email: gareth(a)stratech.co.uk<mailto:gareth@stratech.co.uk> ---------------------------------------------------- Dr Andreas Bruckbauer FILM facility manager Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 401 +44 (0)20 7594 2023 a.bruckbauer(a)imperial.ac.uk<mailto:a.bruckbauer@imperial.ac.uk> http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ ----------------------------------------------------

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[FILM-Users 00735] Used Zeiss 200 Inverted Microscope
by Bruckbauer, Andreas 22 May '17

22 May '17

FYI: From: Digital Pixel Microscopy and Cell Vibility Solutions [mailto:support@digitalpixel.co.uk] Sent: 22 May 2017 15:50 To: Magee, Tony <t.magee(a)imperial.ac.uk<mailto:t.magee@imperial.ac.uk>> Subject: Used Zeiss 200 Inverted Microscope Used Zeiss Axiovert 200 Manual Stand in Perfect Working Order Objectives 10x/0.3 Plan-Neofluar 20x/0.50 Plan-Neofluar 63x/0.95 Plan-Neofluar Fluorescence filters DAPI, FITC, GFP Left hand side camera port with 80-20 100-0% splitter Binocular Eyepieces W-PL 10x 23mm LD condenser 0.55 H, Ph1, Ph2, Ph3, DIC, DIC 6 positions Manual XY stage Stage insert for Slides Objective nosepiece 6x encoded with DIC and 0.8w RMS Threads Transmitted light Halogen 12volt 100watt Fluorescence light insert with electronic shutter Field and Aperture sliders Fluorescence filter turret manual 6 positions. 100W HBO Light Source Please forward to any interested parties. Best Regards Dileni Siri Digital Pixel Cell Viability and Microscopy Systems Science Park Square Brighton BN1 9SB Tel: 0044 1273 502176 Fax: 0044 1273 704499 www.digitalpixel.co.uk<http://www.digitalpixel.co.uk> www.microscopeheaters.com<http://www.microscopeheaters.com>

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[FILM-Users 00734] FILM microscopy day 2017
by Bruckbauer, Andreas 22 May '17

22 May '17

Dear all, The FILM microscopy day 2017 will be held on Tuesday June 27th, a day full of microscopy for everyone, from beginners to experts. As every year we will give you the opportunity to present your work in short presentations (5 minutes)! Please contact me if you wish to contribute. Best wishes Andreas ---------------------------------------------------- Dr Andreas Bruckbauer FILM facility manager Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 401 +44 (0)20 7594 2023 a.bruckbauer(a)imperial.ac.uk<mailto:a.bruckbauer@imperial.ac.uk> http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ ----------------------------------------------------

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[FILM-Users 00733] !!! New WF1 microscope
by Bruckbauer, Andreas 05 May '17

05 May '17

Dear all, The new WF1 microscope was installed today, it is now very similar to the WF3 using Zen software. Steve did the initial setup so that it can be used. WF1 users please contact Steve or myself if you plan to use it so that we can set you up on the system. Best wishes Andreas ---------------------------------------------------- Dr Andreas Bruckbauer FILM facility manager Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 401 +44 (0)20 7594 2023 a.bruckbauer(a)imperial.ac.uk<mailto:a.bruckbauer@imperial.ac.uk> http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ ----------------------------------------------------

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[FILM-Users 00732] FW: RMS Scientific Imaging Competition 2017 - Deadline Approaching
by Bruckbauer, Andreas 24 Apr '17

24 Apr '17

FYI: RMS Scientific Imaging Competition -----Original Message----- From: Royal Microscopical Society [mailto:reply=rmsnewsletter.org.uk@mail166.atl221.rsgsv.net] On Behalf Of Royal Microscopical Society Sent: 24 April 2017 08:01 To: Bruckbauer, Andreas <a.bruckbauer(a)imperial.ac.uk> Subject: RMS Scientific Imaging Competition 2017 - Deadline Approaching You have until midnight on Friday 28 April to submit your entry to the Royal Microscopical Society's International Scientific Imaging Competition. See http://www.rms.org.uk/imaging-competition The competition is a great opportunity for microscopists from across the world to showcase their skills - the 2015 competition attracted interest from BBC Earth and The Times. It provides a great outlet for those that can capture the breath-taking and engaging beauty of the microscopical world. Entries are invited to the following categories: - Electron Microscopy - Physical Science - Electron Microscopy - Life Science - Light Microscopy - Physical Science - Light Microscopy - Life Science - Scanning Probe Microscopy - Short Video There are great prizes on offer including two Nikon cameras. For full details on how to enter, visit - http://www.rms.org.uk/imaging-competition If you would like to sponsor a prize, contact dawn(a)rms.org.uk "This competition goes way beyond 'coffee table' pictures and this makes it special," commented Professor Chris Hawes, former President of the Society and competition judge. "We look for images that have a scientific quality as well as outstanding aesthetic appeal. This provides us with truly eye-catching displays." An exhibition of short-listed entries will be on throughout mmc2017 with the winners being announced during the event. ============================================== You are receiving this email because you are a valued contact of the Royal Microscopical Society. If you do not wish to receive further correspondence, use the unsubscribe facility below. Unsubscribe a.bruckbauer(a)imperial.ac.uk from this list: http://rms.us2.list-manage.com/unsubscribe?u=09d07c67a261f2ebc6e211496&id=7… Our mailing address is: Royal Microscopical Society 37/38 St Clements Street Oxford, OXON OX4 1AJ United Kingdom Our telephone: +44 (0)1865 254760 Forward this email to a friend: http://us2.forward-to-friend1.com/forward?u=09d07c67a261f2ebc6e211496&id=28… Update your profile: http://rms.us2.list-manage.com/profile?u=09d07c67a261f2ebc6e211496&id=736ed…

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[FILM-Users 00731] FW: Call for Proposal: Advanced Imaging Center at Janelia
by Bruckbauer, Andreas 19 Apr '17

19 Apr '17

Interesting opportunity to use Janelia’s advanced microscopes, e.g. the Lattice Light Sheet Microscope or the iPALM. They even pay the cost of lodging for the visiting scientist, basic experimental reagents, technical support from our applications scientists, and scope time! -----Original Message----- From: Chew, Teng-Leong <chewt(a)JANELIA.HHMI.ORG<mailto:chewt@JANELIA.HHMI.ORG>> To: CONFOCALMICROSCOPY <CONFOCALMICROSCOPY(a)LISTS.UMN.EDU<mailto:CONFOCALMICROSCOPY@LISTS.UMN.EDU>> Sent: Mon, 17 Apr 2017 20:21 Subject: Call for Proposal: Advanced Imaging Center at Janelia Dear Colleagues, We are now accepting proposals from scientists who would like to conduct experiments at the Advanced Imaging Center (https://janelia.org/aic) (AIC) at Janelia Research Campus. The deadline to submit a proposal is June 15, 2017. Application Details (http://tinyurl.com/m6r7h7p) The AIC makes imaging technologies developed at Janelia available to the scientific community before the instruments can be purchased commercially. Scientific investigators from nonprofit organizations anywhere in the world may apply to use the facility. We encourage applications from researchers who are addressing significant scientific questions that require measurements of cellular/molecular behavior at spatial and/or temporal resolutions that would only be possible for them through access to the AIC. Under the program, visiting scientists will spend 1-2 weeks (or longer if there is justified scientific reason) at Janelia to conduct experiments on a microscope with the support of the AIC team. The currently available microscopes are: • Interferometric Photoactivated Localization Microscope (iPALM) • Aberration-corrected Multifocus Microscope (acMFM) • Lattice Light Sheet Microscope (2nd generation Bessel Beam Microscope) • Live Cell Multicolor Structured Illumination Microscope (SIM) The AIC is jointly sponsored by the Howard Hughes Medical Institute and the Gordon and Betty Moore Foundation (GBMF). Proposals will be evaluated based on scientific merit through a two-tier review process by AIC/Janelia scientists and by a review panel of scientists from HHMI/Janelia, GBMF, and extramural imaging experts. Scientists whose proposals are selected are responsible for travel arrangements to Janelia. The Janelia Visitor Program will cover the cost of lodging for the visiting scientist, basic experimental reagents, technical support from our applications scientists, and scope time. Email aic(a)janelia.hhmi.org<mailto:aic@janelia.hhmi.org> or me with direct inquiries. Please help us by spreading the word to your colleagues. Additional information about the instruments and instructions on how to apply is available on the AIC webpage (https://janelia.org/aic) You can also download instructions(pdf) (http://tinyurl.com/kthw8en) Regards, Leong --- Teng-Leong Chew, PhD Director, Advanced Imaging Center Janelia Research Campus Howard Hughes Medical Institute Ashburn, VA 20147 (571) 209-4676 chewt(a)janelia.hhmi.org<mailto:chewt@janelia.hhmi.org>

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[FILM-Users 00730] IMPORTANT!!! Switch to PPMS from May 1st
by Keller, Debora 19 Apr '17

19 Apr '17

Dear FILM users >From May 1st, all FILM bookings have to be made via PPMS: https://ppms.eu/imperial/start/ If you haven't done so already, please create an account on PPMS and create a new project (aka grant) for FILM recharging (see below and quickstart guide). -------------------------------------------------------------- If not done already - Takes ~ 5-10min (tops) Would all active users: 1) please create an Account on PPMS 2) VIP: Please also go to "request a new project" and fill the document "Account for recharging (Default)" to input the financial information on PPMS (required before being able to book). see quickstart guide > if you already have an account with FLOW or CSB, please create a project with the grant to be used for FILM recharging. I have attached a quickstart guide how to, but the website should be pretty self-explanatory. In any cases, if you have any questions, do send me an email (d.keller(a)imperial.ac.uk<mailto:d.keller@imperial.ac.uk>). ------------------------------------------------------------- >From May 1st - Bookings are to be made on PPMS We will switch all FILM instruments onto this booking system and deactivate Sharepoint, so make sure you have created your account and project. Any issues with PPMS, please contact FILM staff. Cheers Debbi Debora Keller, PhD FILM - Facility for Imaging by Light Microscopy - Facility Manager - Super-Resolution Specialist - Sir Alexander Fleming Building, desk 408 Imperial College London / South Kensington Exhibition Road London SW7 2AZ, UK Phone: +44 (0)207 594 2023 Mobile: + 44(0) 7760 256 889 E-mail: d.keller(a)imperial.ac.uk<https://exchange.imperial.ac.uk/owa/redir.aspx?C=ly2HSraUakenznQlUce8xI9f3N…> Website: http://imperial.ac.uk/imagingfacility<https://exchange.imperial.ac.uk/owa/redir.aspx?C=ly2HSraUakenznQlUce8xI9f3N…>

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