[FILM-Users 00372] FILM Microscopy Day 2013 - 28 June 2013
Dear microscopists, we have now finalised the programme of this year's FILM Microscopy Day, and I'm pleased to announce that we will finish the day with a plenary lecture given by Spencer Shorte from the Institute Pasteur in Paris on a new technique for intravital imaging. So don't miss out on an interesting day! Those who have attended previous years will know that this is an excellent opportunity to get answers to those questions you always had about microscopy. But even if you have participated previously, we have added new topics - and after a successful start last year, the *Microscopy Match Maker *is back - an excellent opportunity to form collaborations, get ideas and feedback, or just see what other people are doing. The presentations are only 3 minutes / max. 3 slides, so hard work to prepare, so please make sure you put your name down. And supervisors, please encourage your students and postdocs to participate, to show their / your research and connect with other labs. Please registrater NOW by email to <film-service@imperial.ac.uk>, stating whether you will contribute a presentation for the Microscopy Match Maker, a poster, (ideally) both, or just come and listen. *Registration is free*, but we need numbers for catering and organisation. See you all there, good imaging, Martin ------------------------------------------------------------------------ *FILM MICROSCOPY DAY 2013** **Friday 28 June 2013** **G34** *** ------------------------------------------------------------------------ PROGRAMME: ------------------------------------------------------------------------ Morning session *10.00-11.00 Basics of microscopy (Martin Spitaler, FILM)* magnification, properties of light, resolution, transmitted light contrast (PH, DIC, darkfield), epifluorescence, confocal microscopy *11.00-11.45 Live imaging techniques (Mark Scott, FILM) * Multiphoton, in vivo imaging, second harmonic generation (SHG), photobleaching, phototoxicity *11.45-12.00 coffee & tea break* MICROSCOPY MATCH-MAKER *12.000-13.00 Microscopy Match-Maker:* FILM users present their research performed in FILM through 3 min snapshot presentations *13.00-14.00 Poster session with sandwich lunch* Afternoon session *14.00-14.45 A walk through the zoo: Molecular imaging tools (Martin Spitaler, FILM)* FRAP, FLIP, FLIM, FRET, FCS, FUEL, BRET and more *14.45-15.30 Super-resolution microscopy (Steve Rothery, FILM) * TIRF, PALM, STORM *15.30-15.45 coffee & tea break* *15.45-16.30 Understanding images (Luis Pizarro, Computing) * basics of image data analysis, like noise removal, segmentation, tracking, registration PLENARY LECTURE *16.30-17.30 Plenary talk: Spencer Shorte (Institute Pasteur, Paris): "Deep-tissue intravital imaging using bioluminescence excitation of nanoparticles"* (title to be confirmed) The plenary lecture will be followed by drinks and nibbles ------------------------------------------------------------------------ *Martin Spitaler, PhD* *FILM - Facility for Imaging by Light Microscopy* - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler@imperial.ac.uk <mailto:m.spitaler@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility
...just a reminder about next week's microscopy day; registration is free but required, so please register *by Tue 24 June* latest, Martin -------- Original Message -------- Subject: FILM Microscopy Day 2013 - 28 June 2013 Date: Tue, 11 Jun 2013 10:30:52 +0100 From: Martin Spitaler <m.spitaler@imperial.ac.uk> Reply-To: FILM Service <film-service@imperial.ac.uk> To: FILM-Users mailing list <film-users@imperial.ac.uk> CC: Spencer Shorte <sshorte@pasteur.fr> Dear microscopists, we have now finalised the programme of this year's FILM Microscopy Day, and I'm pleased to announce that we will finish the day with a plenary lecture given by Spencer Shorte from the Institute Pasteur in Paris on a new technique for intravital imaging. So don't miss out on an interesting day! Those who have attended previous years will know that this is an excellent opportunity to get answers to those questions you always had about microscopy. But even if you have participated previously, we have added new topics - and after a successful start last year, the *Microscopy Match Maker *is back - an excellent opportunity to form collaborations, get ideas and feedback, or just see what other people are doing. The presentations are only 3 minutes / max. 3 slides, so hard work to prepare, so please make sure you put your name down. And supervisors, please encourage your students and postdocs to participate, to show their / your research and connect with other labs. Please registrater NOW by email to <film-service@imperial.ac.uk>, stating whether you will contribute a presentation for the Microscopy Match Maker, a poster, (ideally) both, or just come and listen. *Registration is free*, but we need numbers for catering and organisation. See you all there, good imaging, Martin ------------------------------------------------------------------------ *FILM MICROSCOPY DAY 2013** **Friday 28 June 2013** **G34** *** ------------------------------------------------------------------------ PROGRAMME: ------------------------------------------------------------------------ Morning session *10.00-11.00 Basics of microscopy (Martin Spitaler, FILM)* magnification, properties of light, resolution, transmitted light contrast (PH, DIC, darkfield), epifluorescence, confocal microscopy *11.00-11.45 Live imaging techniques (Mark Scott, FILM) * Multiphoton, in vivo imaging, second harmonic generation (SHG), photobleaching, phototoxicity *11.45-12.00 coffee & tea break* MICROSCOPY MATCH-MAKER *12.000-13.00 Microscopy Match-Maker:* FILM users present their research performed in FILM through 3 min snapshot presentations *13.00-14.00 Poster session with sandwich lunch* Afternoon session *14.00-14.45 A walk through the zoo: Molecular imaging tools (Martin Spitaler, FILM)* FRAP, FLIP, FLIM, FRET, FCS, FUEL, BRET and more *14.45-15.30 Super-resolution microscopy (Steve Rothery, FILM) * TIRF, PALM, STORM *15.30-15.45 coffee & tea break* *15.45-16.30 Understanding images (Luis Pizarro, Computing) * basics of image data analysis, like noise removal, segmentation, tracking, registration PLENARY LECTURE *16.30-17.30 Plenary talk: Spencer Shorte (Institute Pasteur, Paris): "Deep-tissue intravital imaging using bioluminescence excitation of nanoparticles"* (title to be confirmed) The plenary lecture will be followed by drinks and nibbles ------------------------------------------------------------------------ *Martin Spitaler, PhD* *FILM - Facility for Imaging by Light Microscopy* - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler@imperial.ac.uk <mailto:m.spitaler@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility
...just a reminder about TOMORROW'S Microscopy Day, a few places are still available (also for Microscopy Match Maker presentations), Martin ------------------------------------------------------------------------ Dear microscopists, we have now finalised the programme of this year's FILM Microscopy Day, and I'm pleased to announce that we will finish the day with a plenary lecture given by Spencer Shorte from the Institute Pasteur in Paris on a new technique for intravital imaging. So don't miss out on an interesting day! Those who have attended previous years will know that this is an excellent opportunity to get answers to those questions you always had about microscopy. But even if you have participated previously, we have added new topics - and after a successful start last year, the *Microscopy Match Maker *is back - an excellent opportunity to form collaborations, get ideas and feedback, or just see what other people are doing. The presentations are only 3 minutes / max. 3 slides (incl. title slide) - not much work to prepare - so please make sure you put your name down. And supervisors, please encourage your students and postdocs to participate, to show their / your research and connect with other labs. Please register NOW by email to <film-service@imperial.ac.uk>, stating whether you will contribute a presentation for the Microscopy Match Maker, a poster, (ideally) both, or just come and listen. *Registration is free*, but we need numbers for catering and organisation. See you all there, good imaging, Martin ------------------------------------------------------------------------ *FILM MICROSCOPY DAY 2013** **Friday 28 June 2013** **G34** *** ------------------------------------------------------------------------ PROGRAMME: ------------------------------------------------------------------------ Morning session *10.00-11.00 Basics of microscopy (Martin Spitaler, FILM)* magnification, properties of light, resolution, transmitted light contrast (PH, DIC, darkfield), epifluorescence, confocal microscopy *11.00-11.45 Live imaging techniques (Mark Scott, FILM) * Multiphoton, in vivo imaging, second harmonic generation (SHG), photobleaching, phototoxicity *11.45-12.00 coffee & tea break* MICROSCOPY MATCH-MAKER *12.000-13.00 Microscopy Match-Maker:* FILM users present their research performed in FILM through 3 min snapshot presentations *13.00-14.00 Poster session with sandwich lunch* Afternoon session *14.00-14.45 A walk through the zoo: Molecular imaging tools (Martin Spitaler, FILM)* FRAP, FLIP, FLIM, FRET, FCS, FUEL, BRET and more *14.45-15.30 Super-resolution microscopy (Steve Rothery, FILM) * TIRF, PALM, STORM *15.30-15.45 coffee & tea break* *15.45-16.30 Understanding images (Luis Pizarro, Computing) * basics of image data analysis, like noise removal, segmentation, tracking, registration PLENARY LECTURE *16.30-17.30 Plenary talk: Spencer Shorte (Institute Pasteur, Paris): "Deep-tissue intra-vital imaging using bioluminescence excitation of nanoparticles"* The plenary lecture will be followed by refreshments ------------------------------------------------------------------------ *Martin Spitaler, PhD* *FILM - Facility for Imaging by Light Microscopy* - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler@imperial.ac.uk <mailto:m.spitaler@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility
Dear microscopists, here the latest update on today's FILM Microscopy Day, including *Spencer Shorte's invited talk: "FUEL for thought: a new method for detection of spatial proximity on mesoscopic scales in vitro and in vivo using luminescence excitation"* (4.30pm, G34, see abstract below) and the list of short presentations during the Microscopy Match Maker. See you there, Martin ------------------------------------------------------------------------ *FILM MICROSCOPY DAY 2013** **Friday 28 June 2013** **G34** *** ------------------------------------------------------------------------ PROGRAMME: ------------------------------------------------------------------------ Morning session *10.00-11.00 Basics of microscopy (Martin Spitaler, FILM)* magnification, properties of light, resolution, transmitted light contrast (PH, DIC, darkfield), epifluorescence, confocal microscopy *11.00-11.45 Live imaging techniques (Mark Scott, FILM) * Multiphoton, in vivo imaging, second harmonic generation (SHG), photobleaching, phototoxicity *11.45-12.00 coffee & tea break* MICROSCOPY MATCH-MAKER *12.000-13.00 Microscopy Match-Maker:* FILM users present their research performed in FILM through 3 min snapshot presentations *Alejandra Garcia-Cattaneo* Ajuba is a key component for adherens junction maintenance *Ciro Chiappini* Combining Confocal and Electron Microscopy to investigate the cell-nanoneedle interface *Edwin Hawkins* *Jill Johnson* 3D imaging of airway remodelling in asthmatic mice *Kevin Woollard* Study of in-vivo monocyte and macrophage biology by intravital microscopy *Kim Jonas* The Use of simultaneous dual colour PALM for imaging of receptor complexes *Reema Khorshed* Computational analysis of in vivo images *Ryan Robinson* Ca2+ Imaging of Guinea Pig Ganglia Neurons -- Investigating the effects of Diesel Exhaust Particles (DEP) *Sean Warren* FLIMFit: Rapid Global Analysis of FLIM Microscopy Data *Sophie Schobesberger* *Sowmya Purushothaman* *William Jackson* Tracking blood monocyte motility in vitro *13.00-14.00 Sandwich lunch* Afternoon session *14.00-14.45 A walk through the zoo: Molecular imaging tools (Martin Spitaler, FILM)* FRAP, FLIP, FLIM, FRET, FCS, FUEL, BRET and more *14.45-15.30 Super-resolution microscopy (Steve Rothery, FILM) * TIRF, PALM, STORM *15.30-15.45 coffee & tea break* *15.45-16.30 Understanding images (Luis Pizarro, Computing) * basics of image data analysis, like noise removal, segmentation, tracking, registration PLENARY LECTURE *16.30-17.30 Plenary talk: Spencer Shorte (Institute Pasteur, Paris): "FUEL for thought: a new method for detection of spatial proximity on mesoscopic scales in vitro and in vivo using luminescence excitation"* Bioluminescence imaging is a powerful technique facilitating a broad range of applications from cell-based assay to in vivo analysis in living, intact organisms. The current state of the art uses Bioluminescence Resonance Energy Transfer (BRET) to improve the sensitivity of bioluminescence by red-shifting blue photons, and providing a measure of molecular co-localization at distances of up to 10nm. However, BRET detection methods may overlook long-distance, radiating energy excitation-emission effects that are significant in the bioluminescent detection regime (1). Fluorescence by Unbound Excitation from Luminescence (FUEL) describes this radiating luminescence effect that excites fluorophores by epifluorescence at distances far beyond 10nm, many microns, or even millimeters away in a manner completely distinct from BRET. Further, we show that detection of FUEL per se is sufficient to provide a detection of long-distance proximity in, and beyond the microscopic range both in vitro and in vivo. By enabling detection of mesoscopic proximity between luminescent and fluorescent probes in the context of living biological tissues FUEL promises utility as a novel tool for high-content analysis in cell and animal models. 1. Dragavon J, Blazquez S, Rekiki A, Samson C, Theodorou I, Rogers KL, Tournebize R, Shorte SL. In vivo excitation of nanoparticles using luminescent bacteria. Proc Natl Acad Sci U S A. 2012 Jun 5;109(23):8890-5. The plenary lecture will be followed by refreshments ------------------------------------------------------------------------ *Martin Spitaler, PhD* *FILM - Facility for Imaging by Light Microscopy* - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler@imperial.ac.uk <mailto:m.spitaler@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility
participants (1)
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Martin Spitaler