Dear microscopists,

we have now finalised the programme of this year's FILM Microscopy Day, and I'm pleased to announce that we will finish the day with a plenary lecture given by Spencer Shorte from the Institute Pasteur in Paris on a new technique for intravital imaging. So don't miss out on an interesting day! Those who have attended previous years will know that this is an excellent opportunity to get answers to those questions you always had about microscopy.

But even if you have participated previously, we have added new topics - and after a successful start last year, the Microscopy Match Maker is back - an excellent opportunity to form collaborations, get ideas and feedback, or just see what other people are doing. The presentations are only 3 minutes / max. 3 slides, so hard work to prepare, so please make sure you put your name down. And supervisors, please encourage your students and postdocs to participate, to show their / your research and connect with other labs.

Please registrater NOW by email to <film-service@imperial.ac.uk>, stating whether you will contribute a presentation for the Microscopy Match Maker, a poster, (ideally) both, or just come and listen. Registration is free, but we need numbers for catering and organisation.

See you all there, good imaging,

Martin

FILM MICROSCOPY DAY 2013
Friday 28 June 2013
G34

PROGRAMME:

Morning session

10.00-11.00 Basics of microscopy (Martin Spitaler, FILM)
magnification, properties of light, resolution, transmitted light contrast (PH, DIC, darkfield), epifluorescence, confocal microscopy

11.00-11.45 Live imaging techniques (Mark Scott, FILM)
Multiphoton, in vivo imaging, second harmonic generation (SHG), photobleaching, phototoxicity

11.45-12.00 coffee & tea break

MICROSCOPY MATCH-MAKER

12.000-13.00 Microscopy Match-Maker:
FILM users present their research performed in FILM
through 3 min snapshot presentations

13.00-14.00 Poster session with sandwich lunch

Afternoon session

14.00-14.45 A walk through the zoo: Molecular imaging tools (Martin Spitaler, FILM)
FRAP, FLIP, FLIM, FRET, FCS, FUEL, BRET and more

14.45-15.30 Super-resolution microscopy (Steve Rothery, FILM)
TIRF, PALM, STORM

15.30-15.45 coffee & tea break

15.45-16.30 Understanding images (Luis Pizarro, Computing)
basics of image data analysis, like noise removal, segmentation, tracking, registration

PLENARY LECTURE

16.30-17.30         Plenary talk:
                                Spencer Shorte (Institute Pasteur, Paris):
                                "Deep-tissue intravital imaging using bioluminescence excitation of nanoparticles" (title to be confirmed)

The plenary lecture will be followed by drinks and nibbles

Martin Spitaler, PhD

FILM - Facility for Imaging by Light Microscopy
- Facility Manager -
Sir Alexander Fleming Building, desk 401
Imperial College London / South Kensington
Exhibition Road
London SW7 2AZ
UK

Tel. +44-(0)20-759-42023
E-mail m.spitaler@imperial.ac.uk
Website: http://imperial.ac.uk/imagingfacility