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*From:* Nature Publishing Group <Nature.Publishing.Group@ealert.nature.com <mailto:Nature.Publishing.Group@ealert.nature.com>> *Date:* 29 November 2013 10:03:37 GMT *To:* <t.magee@imperial.ac.uk <mailto:t.magee@imperial.ac.uk>> *Subject:* *On Demand webcast: Real time imaging of cells with Super Resolution Microscopy.* *Reply-To:* <Nature.Publishing.Group@ealert.nature.com <mailto:Nature.Publishing.Group@ealert.nature.com>>

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*The latest innovations **in real-time imaging of cells with Super Resolution Microscopy **

A discussion into new technical innovations for Super Resolution Microscopy**

Now available On Demand *

** Speakers: *Dr Jennifer Lippincott-Schwartz* NIH Distinguished Investigator.

*Dr Orla Hanrahan* Andor Technology.

*Dr Susan Cox* King's College, London.

*Register for FREE <http://links.ealert.nature.com/ctt?kn=20&ms=NDQ0NjkyOTgS1&r=MTc3MDMyNTQyMwS2&b=0&j=MjE1Nzg1NTA5S0&mt=1&rt=0>*

Due to the diffraction limit, standard light microscopes can at best resolve features which are around 200nm apart, meaning that they are unsuited to many biological applications. In this webcast, three expert speakers will discuss super resolution microscopy, a rapidly developing field that is breaking the diffraction limit to enable the close examination of finely detailed biological samples.

The current focus of the Super Resolution Microscopy community is to increase the utility of existing localisation microscopy techniques such as photo-activated localization microscopy (PALM) and stochastic optical reconstruction microscopy (STORM) – in these methods, fluorophores within a sample emit low levels of light, which is imaged many thousands of times. This creates a single composite image with resolution at the true molecular length scale. However, this process is slow, meaning that these techniques are not best suited to real-time imaging of cells and tissues.

Our first speaker, Dr Jennifer Lippincott-Schwartz, will discuss research currently underway using genetically-encoded photoactivatable proteins (PA-FPs) for superresolution imaging which has dramatically expanded the study of cellular organization, function and dynamics. Our second speaker, Dr Susan Cox will go into some detail on the algorithms which facilitate high resolution imaging of live cell dynamics at the nanoscale and lastly Dr Orla Hanrahan will discuss fast and sensitive detector technologies which are suitable for the low light intensities of the single molecule techniques and the speed required for live cell super resolution applications.

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