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Speakers:
Dr Jennifer
Lippincott-Schwartz
NIH Distinguished Investigator.
Dr Orla Hanrahan
Andor Technology.
Dr Susan Cox
King's College, London.
Register for FREE
Due to the diffraction limit,
standard light microscopes can at
best resolve features which are
around 200nm apart, meaning that
they are unsuited to many biological
applications. In this webcast, three
expert speakers will discuss super
resolution microscopy, a rapidly
developing field that is breaking
the diffraction limit to enable the
close examination of finely detailed
biological samples.
The current focus of the Super
Resolution Microscopy community is
to increase the utility of existing
localisation microscopy techniques
such as photo-activated localization
microscopy (PALM) and stochastic
optical reconstruction microscopy
(STORM) – in these methods,
fluorophores within a sample emit
low levels of light, which is imaged
many thousands of times. This
creates a single composite image
with resolution at the true
molecular length scale. However,
this process is slow, meaning that
these techniques are not best suited
to real-time imaging of cells and
tissues.
Our first speaker, Dr Jennifer
Lippincott-Schwartz, will discuss
research currently underway using
genetically-encoded photoactivatable
proteins (PA-FPs) for
superresolution imaging which has
dramatically expanded the study of
cellular organization, function and
dynamics. Our second speaker, Dr
Susan Cox will go into some detail
on the algorithms which facilitate
high resolution imaging of live cell
dynamics at the nanoscale and lastly
Dr Orla Hanrahan will discuss fast
and sensitive detector technologies
which are suitable for the low light
intensities of the single molecule
techniques and the speed required
for live cell super resolution
applications.
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