...forwarded for your information, Martin -------- Original Message -------- Subject: Join our free webinar: Latest Biological Applications for 3D Super-resolution Microscopy Date: Mon, 3 Feb 2014 16:03:18 +0100 From: Leica Microsystems <news.eu@leica-microsystems.com> Reply-To: Leica Microsystems <nina.brauns@leica-microsystems.com> To: <m.spitaler@imperial.ac.uk> Click here to view this e-mail as webpage <http://rdir.inxmail.com/leica/d?q000nmoi00s46s00d0000000000000000cttcn3i38¶ms=309737+m.spitaler%40imperial.ac.uk+0+000buiq0000cm0000000000u4zx2elnj> <http://rdir.inxmail.com/leica/d?q000nmqi00s46s00d0000000000000000cttbzki38&nlc=20140131-DEWE-9FVLW2> Dear Scientist, *FREE Online Web Seminar** Wednesday February 19, 2014* 08:00 PST | 11:00 EST | 16:00 GMT | 17:00 CET *Click here to register* <http://rdir.inxmail.com/leica/d?q000nmq000s46s00d0000000000000000cttkus038&nlc=20140131-DEWE-9FVLW2> Leica Microsystems, in conjunction with Microscopy and Analysis, are pleased to announce a webinar highlighting ground-breaking techniques in microscopy. Dr. Timo Zimmermann and Dr. Eric Hosy will present the impressive results they have achieved using confocal and widefield 3D super-resolution methods. Biological application for the next generaton of STED microscopy systems Stimulated Emission Depletion (STED) constitutes a powerful method for resolution improvement beyond the diffraction in light microscopy. Additional time-gating (gSTED) of the detection signal can be used in systems with continuous wave (CW) laser depletion to improve the image resolution even further. Until now, these improvements were limited in gated CW-STED systems to the lateral resolution of the image and to the depletion of green fluorophores at 592nm. The now introduced possibility to improve axial resolution and the introduction of an additional laser line for depletion at 660nm offer new ways to image biological samples. We have taken highly resolved 3D and multichannel datasets of a variety of specimens and structures to make full use of these new features. In the talk, several projects will be covered that make use of the improved z-resolution and that use orange to red dyes and dye combinations for the new depletion laser line. <http://rdir.inxmail.com/leica/d?q000nmqi00s46s00d0000000000000000cttbzki38&nlc=20140131-DEWE-9FVLW2> *Speaker: Dr. Timo Zimmermann*, Head of the Advanced Light Microscopy Unit, CRG-Centre for Genomic Regulation, Spain Live cell localization microscopy with Leica SR GSD 3D Brownian diffusion tends to homogenize proteins that should diffuse rapidly inside the cytoplasm or cell surface. Protein clustering or immobilization necessitates specific molecular organization which counteracts this phenomenon. During the last ten years, knowledge about the role and properties of protein diffusion made a big step forward thanks to the development of single molecule and Quantum dot tracking (SPT). However the sparse labelling inherent to these techniques render it impossible to give an overview of the protein organization. We will report, in this webinar, the historic, the interest and the proper use of a new optical technique allowing users to obtain a high number of single molecule trajectories at the surface of a living cell using the Leica SR GSD 3D system. This versatile method, developed in 2010 and called U-PAINT, is suitable for studying any membrane biomolecule (endogenous or transfected) and provides dynamical information with large statistics revealing localization specific diffusion properties of a molecule on a single cell. <http://rdir.inxmail.com/leica/d?q000nmqi00s46s00d0000000000000000cttbzki38&nlc=20140131-DEWE-9FVLW2> *Speaker: Dr. Eric Hosy*, CNRS, University of Bordeaux, France Imprint Leica Mikrosysteme Vertrieb GmbH, Ernst-Leitz-Straße 17-37, 35578 Wetzlar Phone: +49 6441 29-4000, Fax: +49 6441 29-4155, Website: www.leica-microsystems.com Email: news_eu@leica-microsystems.com Managing Director: Frank Bunge, Colin Davis, Cornelis Johannes Maria van Ophem Trade register number: Wetzlar HRB 5464, VAT number: DE 811140297 Leica Microsystems owns the copyright and is responsible for all contents and data. Subsequent utilization of text, data and pictures only with prior permission. Click here <http://rdir.inxmail.com/leica/d/d.php?q000nmny00s46s00k0000000000000000ctt055i38&nlc=20140131-DEWE-9FVLW2> if you want to unsubscribe. <http://rdir.inxmail.com/leica/d?q000nmpi00s46s00d0000000000000000cttpd2y38&nlc=20140131-DEWE-9FVLW2> <http://rdir.inxmail.com/leica/d?q000nmpq00s46s00d0000000000000000cttczci38&nlc=20140131-DEWE-9FVLW2> <http://rdir.inxmail.com/leica/d?q000nmpy00s46s00d0000000000000000cttju2038&nlc=20140131-DEWE-9FVLW2>