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Dear
Scientist,
FREE
Online Web Seminar
Wednesday
February 19, 2014
08:00
PST | 11:00 EST | 16:00 GMT |
17:00 CET
Click
here to register
Leica
Microsystems, in conjunction
with Microscopy and Analysis,
are pleased to announce a
webinar highlighting
ground-breaking techniques in
microscopy. Dr. Timo Zimmermann
and Dr. Eric Hosy will present
the impressive results they have
achieved using confocal and
widefield 3D super-resolution
methods.
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Biological
application for the next generaton of
STED microscopy systems |
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Stimulated
Emission Depletion (STED)
constitutes a powerful method
for resolution improvement
beyond the diffraction in light
microscopy.
Additional
time-gating (gSTED) of the
detection signal can be used in
systems with continuous wave
(CW) laser depletion to improve
the image resolution even
further.
Until
now, these improvements were
limited in gated CW-STED systems
to the lateral resolution of the
image and to the depletion of
green fluorophores at 592nm. The
now introduced possibility to
improve axial resolution and the
introduction of an additional
laser line for depletion at
660nm offer new ways to image
biological samples. We have
taken highly resolved 3D and
multichannel datasets of a
variety of specimens and
structures to make full use of
these new features.
In
the talk, several projects will
be covered that make use of the
improved z-resolution and that
use orange to red dyes and dye
combinations for the new
depletion laser line.
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| Speaker:
Dr. Timo Zimmermann,
Head of the Advanced
Light Microscopy Unit,
CRG-Centre for Genomic
Regulation, Spain |
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Live
cell localization microscopy with Leica
SR GSD 3D |
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Brownian
diffusion tends to homogenize
proteins that should diffuse
rapidly inside the cytoplasm or
cell surface. Protein clustering
or immobilization necessitates
specific molecular organization
which counteracts this
phenomenon.
During
the last ten years, knowledge
about the role and properties of
protein diffusion made a big
step forward thanks to the
development of single molecule
and Quantum dot tracking (SPT).
However the sparse labelling
inherent to these techniques
render it impossible to give an
overview of the protein
organization.
We
will report, in this webinar,
the historic, the interest and
the proper use of a new optical
technique allowing users to
obtain a high number of single
molecule trajectories at the
surface of a living cell using
the Leica SR GSD 3D system. This
versatile method, developed in
2010 and called U-PAINT, is
suitable for studying any
membrane biomolecule (endogenous
or transfected) and provides
dynamical information with large
statistics revealing
localization specific diffusion
properties of a molecule on a
single cell.
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| Speaker:
Dr. Eric Hosy,
CNRS, University of
Bordeaux, France |
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Imprint
Leica
Mikrosysteme Vertrieb GmbH,
Ernst-Leitz-Straße 17-37, 35578
Wetzlar
Phone:
+49 6441 29-4000, Fax: +49 6441
29-4155, Website:
www.leica-microsystems.com
Email:
news_eu@leica-microsystems.com
Managing
Director: Frank Bunge, Colin
Davis, Cornelis Johannes Maria
van Ophem
Trade
register number: Wetzlar HRB
5464, VAT number: DE 811140297
Leica
Microsystems owns the copyright
and is responsible for all
contents and data. Subsequent
utilization of text, data and
pictures only with prior
permission.
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