Dear microscopists, we have now finalised the programme of this year's FILM Microscopy Day, and I'm pleased to announce that we will finish the day with a plenary lecture given by Spencer Shorte from the Institute Pasteur in Paris on a new technique for intravital imaging. So don't miss out on an interesting day! Those who have attended previous years will know that this is an excellent opportunity to get answers to those questions you always had about microscopy. But even if you have participated previously, we have added new topics - and after a successful start last year, the *Microscopy Match Maker *is back - an excellent opportunity to form collaborations, get ideas and feedback, or just see what other people are doing. The presentations are only 3 minutes / max. 3 slides, so hard work to prepare, so please make sure you put your name down. And supervisors, please encourage your students and postdocs to participate, to show their / your research and connect with other labs. Please registrater NOW by email to <film-service@imperial.ac.uk>, stating whether you will contribute a presentation for the Microscopy Match Maker, a poster, (ideally) both, or just come and listen. *Registration is free*, but we need numbers for catering and organisation. See you all there, good imaging, Martin ------------------------------------------------------------------------ *FILM MICROSCOPY DAY 2013** **Friday 28 June 2013** **G34** *** ------------------------------------------------------------------------ PROGRAMME: ------------------------------------------------------------------------ Morning session *10.00-11.00 Basics of microscopy (Martin Spitaler, FILM)* magnification, properties of light, resolution, transmitted light contrast (PH, DIC, darkfield), epifluorescence, confocal microscopy *11.00-11.45 Live imaging techniques (Mark Scott, FILM) * Multiphoton, in vivo imaging, second harmonic generation (SHG), photobleaching, phototoxicity *11.45-12.00 coffee & tea break* MICROSCOPY MATCH-MAKER *12.000-13.00 Microscopy Match-Maker:* FILM users present their research performed in FILM through 3 min snapshot presentations *13.00-14.00 Poster session with sandwich lunch* Afternoon session *14.00-14.45 A walk through the zoo: Molecular imaging tools (Martin Spitaler, FILM)* FRAP, FLIP, FLIM, FRET, FCS, FUEL, BRET and more *14.45-15.30 Super-resolution microscopy (Steve Rothery, FILM) * TIRF, PALM, STORM *15.30-15.45 coffee & tea break* *15.45-16.30 Understanding images (Luis Pizarro, Computing) * basics of image data analysis, like noise removal, segmentation, tracking, registration PLENARY LECTURE *16.30-17.30 Plenary talk: Spencer Shorte (Institute Pasteur, Paris): "Deep-tissue intravital imaging using bioluminescence excitation of nanoparticles"* (title to be confirmed) The plenary lecture will be followed by drinks and nibbles ------------------------------------------------------------------------ *Martin Spitaler, PhD* *FILM - Facility for Imaging by Light Microscopy* - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler@imperial.ac.uk <mailto:m.spitaler@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility