January 2014 - FILM-Users - imperial.ac.uk

[FILM-Users 00455] FILM Club Wed 29 Jan, SAFB-122: Catherine Simpson - Sort your microscopy out!
by Martin Spitaler 23 Jan '14

23 Jan '14

Dear microscopists, the first FILM Club of the new year is coming up next week Wednesday: *Catherine Simpson: "Sort your microscopy out!" Wed 29 Jan, SAFB-122, 4.30pm* Catherine Simpson is the facility manager of the Flow Cytometry Facility in South Kensington (http://www3.imperial.ac.uk/lifesciences/research/facilities/flowcytometry) In this talk, she will go into the very powerful combination of flow cytometry and microscopy to extract much more useful information from biological samples. While flow cytometry is incredibly powerful in quantifying multiple cellular parameters at high speed from large numbers of samples, it's missing one important trick: The cellular context of these parameters in time and space. This is where microscopy excels, it keeps the whole big picture intact, how molecules relate to each other, interact with each other and change over time. But this comes at the price of often lousy statistics, especially for live imaging. And it can take ages to find the 'needle in the haystack', for example a cell of a certain type, transiently transfected or with a certain expression level or combination or fluorophores. Combining the two - analysis and sorting on a FACS, live visualisation and measurement on a microscope - opens up a whole new dimension of understanding. Cells can be analysed in high numbers, rare cells sorted into microscopy multi-well plates or slides, or even re-introduced into live animals and followed up by intravital imaging. Some people ask for the ImageStream technology to analyse their samples - we can do all that and much more, at much higher magnification and over time! See you there, good imaging, Martin -- ------------------------------------------------------------------------ *Martin Spitaler, PhD* *FILM - Facility for Imaging by Light Microscopy* - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk <mailto:m.spitaler@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

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[FILM-Users 00454] FW: HEK293 cells
by Rothery, Stephen M 22 Jan '14

22 Jan '14

Can anyone help Pakatip? From: Ruenraroengsak, Pakatip Sent: 22 January 2014 13:17 To: Rothery, Stephen M Subject: HEK293 cells Dear Stephen Happy New Years 2014. Hope you had great break over the new year. I am looking for HEK293 cells, and wondering if you have them or know someone that I can get the cells. Please let me know. Thank you very much. Dr Pakatip Ruenraroengsak (BSc., MSc., Ph.D.) Research Associate Department of Materials, Faculty of engineering, Imperial college London, Exhibition Rd, SW7 2AZ and Lung Cell Biology, Section of Pharmacology and toxicology, National Heart and Lung Institute, Imperial College London, Dovehouse St, London SW3 6LY

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[FILM-Users 00453] Fwd: Fwd: add
by Martin Spitaler 16 Jan '14

16 Jan '14

...forwarded for your information, the original attachment is at <https://workspace.imperial.ac.uk/imagingfacility/Public/_attachment-140116.…>. I've just been asked to forward this, don't know the lab nor the supervisor, but certainly applying for an imaging job later and having worked in a Nyqvist lab would be eye-catching! Martin ------------------------------------------------------------------------ ----- Weitergeleitete Nachricht vonmartowicz(a)gmail.com ----- Datum: Wed, 15 Jan 2014 14:45:03 +0100 Von: "Agnieszka Martowicz"<martowicz(a)gmail.com> Antwort an: "Agnieszka Martowicz"<martowicz(a)gmail.com> Betreff: add Thanks for forwarding. Aga -- Agnieszka Martowicz, PhD KAROLINSKA INSTITUTET Dept. of Medical Biochemistry and Biophysics Division of Vascular Biology Scheelesv 2, House A3, level 4 17177 Stockholm, Sweden Phone: +46 (0)8 524 879 66 ------------------------------------------------------------------------ Department of Medical Biochmistry and Biophysics Division of Vascular Biology Postdoc in angiogenesis focused on live cell and in vivo confocal imaging Department The Department of Medical Biochemistry and Biophysics conducts multidisciplinary research and education in biomedicine. Presently our department consists of 11 research divisions with a total of approximately 300 employees. The Division of Vascular Biology provides an international and interactive environment with seven research groups; Christer Betsholtz, Ulf Eriksson, Jonas Fuxe, Johan Björkegren, Lars Jakobsson, Guillem Genové and Daniel Nyqvist. Area of research / Research group The research group of D r. Daniel Nyqvist is investigating molecular mechanisms regulating vascular growth and barrier function in health and disease. We study angiogenesis during developmental processes and in pathophysiological conditions such as tumor growth and retinopathy. W e are a young, dynamic and very enthusiastic research team with a well - established collaborative network and strong funding. Please visit the group website: http://ki.se/ki/jsp/polopoly.jsp?d=45 985&l=en The project will be focused on visualizing cellular processes underlying vessel growth and function using one - and two - photon laser - scanning microscopy both in vivo and in live cell cultures. The work will take advantage of established applications for intravital imaging in the anterior chamber of the eye (AC) and ex vivo organ cultures, but also include development of novel methods. A wide range of fluorescent repor ter s and genetically modified mou se models will be used to study vessel growth and function, both in healthy conditions and in disease state s such as tumor angiogenesis. Two new state - of - the - art confocal imaging systems will be available for these studies. Lab r eferences: Nyqvist et al., Diabetes 2011, Corada et al., Dev Cell 2010, Speier, Nyqvist et al., Nat Med 2008. Qualifications of the applicant We are seeking a highly motivated researcher with a good team spirit and deep curiosity for science. The ideal candidate should have a strong background in one - and two - photon microscopy, image processing and animal physiology with a keen interest in vascular biology. Experience in working with confocal microscopy is a must, and experience from working with mice an d molecular biology techniques is a strong plus; and good ability to communicate in English (written and verbal) is required. Candidates will be encouraged to serve as mentors for master - and PhD - students. Type of scholarship Karolinska Institutet awards scholarships to pursue postdoctora l studies. This educational scholarship, which is paid for a maximum of two years within five years after the receipt of a PhD or the equivalent, is tax - exempt. Application process An application must contain the follow ing documents in English: . Personal letter of motivation . Curriculum vitae (max 3 pages) . Complete list of publications . Summary of current work (1 page) . Two reference letter s . Ph.D. degree certificate or a formal document from the dis sertation - committee stating the date of dissertation The application is to be submitted on the NetRecruiter system ; http://ki.se/ki/jsp/polopoly.jsp?l=en&d=469&a=88392 . Starting date is negotiable, but beginning of fall 2014 is preferred. For further information please email to daniel.nyqvist(a)ki.se

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[FILM-Users 00452] Definiens Advanced training course
by Martin Spitaler 15 Jan '14

15 Jan '14

Dear microscopists, we have just confirmed that the DEFINIENS ADVANCED TRAINING COURSE will take place Wed-Fri 5-7 March 2014 in the computer training room of the Central Library. If interested, Proposed topics are OBJECT TRACKING and MACHINE LEARNING, but we have flexibility to adjust to interested participants. If you are interested, please get in touch as soon as possible, to make sure you get a place and we can finalise the programme. And don't miss out, this will be a course tailored to your needs, and even at a heavily subsidised rate (should be ~£400-500). Good imaging, Martin -- ------------------------------------------------------------------------ *Martin Spitaler, PhD* *FILM - Facility for Imaging by Light Microscopy* - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk <mailto:m.spitaler@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

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[FILM-Users 00451] Fwd: Postdoc Opportunity at MedImmune [NC]
by Martin Spitaler 15 Jan '14

15 Jan '14

...forwarded for your information, Martin -------- Original Message -------- Subject: Postdoc Opportunity at MedImmune [NC] Date: Wed, 15 Jan 2014 11:31:51 +0000 From: Alex Knight <alex.knight(a)npl.co.uk> To: undisclosed-recipients:; Classification: NPL Management Ltd - Commercial Dear colleagues, I'd like to let you know about a postdoc opportunity at MedImmune in Cambridge, in collaboration with NPL, in the hope that you may know some suitable candidates. Please feel free to forward on to anyone who you think may be interested. The project is on exosomes and will include the application of super-resolution microscopy to understand how they interact with recipient cells. http://www.candidatecare.com/srccsh/RTI.home?r=2000029244410&c=921&d=medimm… Best Wishes, Alex Alex Knight Principal Research Scientist Biophysics and Diagnostics National Physical Laboratory Hampton Rd | Teddington | Middlesex | UK | TW11 0LW t: 020 8943 6308 m: 0773 8895 810 f: 020 8614 0567 e: alex.knight(a)npl.co.uk w: http://www.npl.co.uk/biotechnology ------------------------------------------------------------------------ If you have received this message in error, please notify us and remove it from your system. NPL Management Ltd cannot guarantee that the e-mail or any attachments are free from viruses. NPL Management Ltd is a company registered in England and Wales, number: 2937881 Registered office: Serco House | 16 Bartley Wood Business Park | Hook, Hampshire | UK | RG27 9UY ------------------------------------------------------------------------ <http://www.npl.co.uk/> STAY CONNECTED <http://www.twitter.com/npl> <http://www.facebook.com/npldigital> <http://www.youtube.com/npldigital> <http://itunes.apple.com/us/podcast/national-physical-laboratory/id295076289> <https://ktn.innovateuk.org/web/measurement-network>

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