January 2014 - FILM-Users - imperial.ac.uk

[FILM-Users 00434] FILM application for Wellcome Trust Multi-User Equipment grant
by Martin Spitaler 31 Mar '14

31 Mar '14

Dear microscopists, following feedback from FILM users and Wellcome Trust grant holders (see summary at the bottom), the FILM steering committee has decided to use the open call for Wellcome Trust Multi-User Equipment grant applications to apply for upgrades / updates of existing equipment as well as some novel technology. The highest demand is currently for a new super-resolution microscope, which is luckily already funded by BBSRC, we are now in the process of testing the equipment to be purchased. Other demands stretched across a wide range of technologies and unfortunately also locations across the college, the committee tried to strike the best balance between all needs and decided to apply for the following package: * _New technology_: o an *automated high-throughput **m**icroscopy* system (e.g. Operetta or similar) o the high-throughput capacity, in combination with existing image processing / analysis capacity (e.g. Definiens) will satisfy the needs for both the high-content screening and to a large degree ImageStream needs * _Upgrade_: o *upgrade of the existing intravital microscope (FILM-CF3)* with options including high-sensitivity hybrid detectors, 405 laser, a cabinet for mouse handling, laser dispersion correction (detailed options to be checked with manufacturers) * _Support_: o *one full-time FILM staff post for Hammersmith* for the duration of the grant * _Replacement_: o *a high-sensitivity new-generation confocal* (similar to the Zeiss LSM-780 in Hammersmith), to be located in SAFB / South Kensington * _Possible options for small, complementary equipment add-ons_: o FleXcell Tensions System o ONIX microfluidic platform (if not already funded by other sources through other sources) o a high-precision multi-channel peristaltic pump system Obviously, we can't apply for everything on the list at the same time, some things will have to wait for the next equipment grant call. We now need to finalise the application - and for that *we need your support*: Those of you willing to support the application with your scientific interest and track record, please do get back to us soon - the deadline for the preliminary application is 2nd of December. And although the major bits of equipment to go on the application have been decided, if there are smaller add-ons missing on the list that would help with your microscopy in FILM, please let us know. Good imaging, Martin _*Information: *_ * http://www.wellcome.ac.uk/Funding/Biomedical-science/Funding-schemes/Strate… _*Summary of **m**icroscopy needs in Imperial College - FILM, feedback 2013*:_

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[FILM-Users 00464] lists of fluorescent proteins
by Martin Spitaler 31 Jan '14

31 Jan '14

Dear microscopists, we frequently get requests for proteins for special applications, like intravital imaging (far-red), sensing (binding specificity), super-resolution (photo-switching) etc. I have just added two excellent, interactive lists to the FILM website > LINKS > GENERAL INFORMATION <http://www3.imperial.ac.uk/portal/page/portallive/imagingfacility/links/ima…>, both hosted at UCSF: * Fluorescent proteins <http://nic.ucsf.edu/FPvisualization/> * Photoswitchable proteins <http://nic.ucsf.edu/FPvisualization/PSFP.html> Worth having a look, there is much more to life (science research) than GFP and CFP. Good imaging, Martin -- ------------------------------------------------------------------------ *Martin Spitaler, PhD* *FILM - Facility for Imaging by Light Microscopy* - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk <mailto:m.spitaler@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

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[FILM-Users 00463] Definiens Advanced training course
by Martin Spitaler 31 Jan '14

31 Jan '14

Dear microscopists, there are still some places left on the DEFINIENS ADVANCED TRAINING COURSE will take place Wed-Fri 5-7 March 2014 in the computer training room of the Central Library. The training will be customised to our needs, the *confirmed topics* (responding to you users) will be: * tracking * machine learning * Image registration * Feature space optimization If interested, please get in touch as soon as possible. And don't forget, this is offered at a discounted rate (thanks to the Imperial College Equipment Sharing grant) of only £400 per person! Good imaging, Martin -- ------------------------------------------------------------------------ *Martin Spitaler, PhD* *FILM - Facility for Imaging by Light Microscopy* - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk <mailto:m.spitaler@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

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[FILM-Users 00462] Fwd: FW: Image competition deadline 1st Feb!!!
by Martin Spitaler 30 Jan '14

30 Jan '14

...forwarded for your information, Martin -------- Original Message -------- Subject: FW: Image competition deadline 1st Feb!!! Date: Thu, 30 Jan 2014 10:37:36 +0000 From: Braga, Vania M M <v.braga(a)imperial.ac.uk> * Subject:* Image competition deadline 1st Feb!!! *Importance:* High Dear all Sorry to hassle you again, but we urgently need submissions for the image competition whose deadline is 1 Feb. So far I only have one entry and that was at the wrong size/resolution. Usually we get 10-20 entries, so I'm unsure what has gone wrong this year. The winners get cash prizes, will appear on the cover of the newsletter and on the new website where they will be featured in a dedicated gallery. *Entry is open to any BSCB member (including lab heads! ) Copyright is retained by the entrant. * *http://www.bscb.org/imagecompetition* * * * * *Also don't forget to encourage your postdoc and lab members to submit a short piece of text for the Writing Prize! * *http://www.bscb.org/sciencewriting* Many thanks Paul ________________________________________________ Dr. Paul. D. Andrews BSc PhD FSB BSCB Public Engagement Coordinator Mobile +44 7590543266 email:pdandrews1@mac.com

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[FILM-Users 00461] TODAY: FILM Club Wed 29 Jan, SAFB-122: Catherine Simpson - Sort your microscopy out!
by Martin Spitaler 29 Jan '14

29 Jan '14

Just a reminder: ------------------------------------------------------------------------ Dear microscopists, the first FILM Club of the new year is coming up next *TODAY*: *Catherine Simpson: "Sort your microscopy out!" Wed 29 Jan, SAFB-122, 4.30pm* Catherine Simpson is the facility manager of the Flow Cytometry Facility in South Kensington (http://www3.imperial.ac.uk/lifesciences/research/facilities/flowcytometry) In this talk, she will go into the very powerful combination of flow cytometry and microscopy to extract much more useful information from biological samples. While flow cytometry is incredibly powerful in quantifying multiple cellular parameters at high speed from large numbers of samples, it's missing one important trick: The cellular context of these parameters in time and space. This is where microscopy excels, it keeps the whole big picture intact, how molecules relate to each other, interact with each other and change over time. But this comes at the price of often lousy statistics, especially for live imaging. And it can take ages to find the 'needle in the haystack', for example a cell of a certain type, transiently transfected or with a certain expression level or combination or fluorophores. Combining the two - analysis and sorting on a FACS, live visualisation and measurement on a microscope - opens up a whole new dimension of understanding. Cells can be analysed in high numbers, rare cells sorted into microscopy multi-well plates or slides, or even re-introduced into live animals and followed up by intravital imaging. Some people ask for the ImageStream technology to analyse their samples - we can do all that and much more, at much higher magnification and over time! See you there, good imaging, Martin -- ------------------------------------------------------------------------ *Martin Spitaler, PhD* *FILM - Facility for Imaging by Light Microscopy* - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk <mailto:m.spitaler@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

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[FILM-Users 00460] TOMORROW: FILM Club Wed 29 Jan, SAFB-122: Catherine Simpson - Sort your microscopy out!
by Martin Spitaler 28 Jan '14

28 Jan '14

Just a reminder: ------------------------------------------------------------------------ Dear microscopists, the first FILM Club of the new year is coming up next *TOMORROW*: *Catherine Simpson: "Sort your microscopy out!" Wed 29 Jan, SAFB-122, 4.30pm* Catherine Simpson is the facility manager of the Flow Cytometry Facility in South Kensington (http://www3.imperial.ac.uk/lifesciences/research/facilities/flowcytometry) In this talk, she will go into the very powerful combination of flow cytometry and microscopy to extract much more useful information from biological samples. While flow cytometry is incredibly powerful in quantifying multiple cellular parameters at high speed from large numbers of samples, it's missing one important trick: The cellular context of these parameters in time and space. This is where microscopy excels, it keeps the whole big picture intact, how molecules relate to each other, interact with each other and change over time. But this comes at the price of often lousy statistics, especially for live imaging. And it can take ages to find the 'needle in the haystack', for example a cell of a certain type, transiently transfected or with a certain expression level or combination or fluorophores. Combining the two - analysis and sorting on a FACS, live visualisation and measurement on a microscope - opens up a whole new dimension of understanding. Cells can be analysed in high numbers, rare cells sorted into microscopy multi-well plates or slides, or even re-introduced into live animals and followed up by intravital imaging. Some people ask for the ImageStream technology to analyse their samples - we can do all that and much more, at much higher magnification and over time! See you there, good imaging, Martin -- ------------------------------------------------------------------------ *Martin Spitaler, PhD* *FILM - Facility for Imaging by Light Microscopy* - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk <mailto:m.spitaler@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

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[FILM-Users 00459] FW: New Webinar - Registration is Now Open for In Vivo Optical Imaging
by Martin Spitaler 27 Jan '14

27 Jan '14

...forwarded for your information, Martin To display this email in a browser, please click here <http://mail.cell-press.com/go.asp?/bECE001/mPMRFU5F/qPDR6U5F/u7NCQ4/x0HUXU5…> Cell Press <http://view6.workcast.net/register?pak=5852298848973576&referrer=email> <http://view6.workcast.net/register?pak=5852298848973576&referrer=email> Register for a Free Webinar ------------------------------------- In Vivo Optical Imaging John S. Condeelis, Michael Cahalan, and Mary Dickinson ------------------------------------- Live date: Tuesday, February 25th, 2014 12:00 pm EST Check the time in your country <http://www.worldtimeserver.com/convert_time_in_GB.aspx?y=2013&mo=5&d=9&h=17…> ------------------------------------- Register Today, It's Free... <http://view6.workcast.net/register?pak=5852298848973576&referrer=email> Please join us to hear John Condeelis, Michael Cahalan, and Mary Dickinson describe the approaches developed in their labs for acquiring and extracting quantitative, multi-dimensional data from living organisms undergoing dynamics associated with cancer, immune response, and development. *Participants will learn about: * * Quantitatively dynamic processes within complicated biological systems. * Multi-dimensional data from living organisms undergoing dynamic processes. * Quantitative measurements of dynamic cellular processes and movements. Register Today, It's Free... <http://view6.workcast.net/register?pak=5852298848973576&referrer=email> Unable to join us on February 25? This webinar will be archived for 6 months for on-demand viewing. Register today <http://view6.workcast.net/register?pak=5852298848973576&referrer=email> to ensure you receive updates on how to gain access after the live event! To register, go to http://bit.ly/1dzbEF5 Speakers: Condeelis <http://view6.workcast.net/register?pak=5852298848973576&referrer=email>*John S. Condeelis * Professor, Anatomy & Structural Biology and Scientific Director, Analytical Imaging Center Albert Einstein College of Medicine *Cahalan <http://view6.workcast.net/register?pak=5852298848973576&referrer=email>Michael Cahalan* Professor and Chair, Physiology and Biophysics University of California, Irvine *Dickinson <http://view6.workcast.net/register?pak=5852298848973576&referrer=email>Mary Dickinson* Professor, Physiology and Biophysics Baylor College of Medicine Moderator: *Piston <http://view6.workcast.net/register?pak=5852298848973576&referrer=email>Dave Piston * Director, Biophotonics Institute, Vanderbilt University School of Medicine Associate Editor, /Biophysical Journal / Supporting Journal: Biophysical Journal <http://www.cell.com/biophysj/home> FaceBook <http://mail.cell-press.com/go.asp?/bECE001/mPMRFU5F/u7NCQ4/x0HUXU5F/cutf%2D…> LinkedIn <http://mail.cell-press.com/go.asp?/bECE001/mPMRFU5F/u7NCQ4/x0HUXU5F/cutf%2D…> twitter <http://mail.cell-press.com/go.asp?/bECE001/mPMRFU5F/u7NCQ4/x0HUXU5F/cutf%2D…> Share by Email <http://mail.cell-press.com/go.asp?/bECE001/mPMRFU5F/u7NCQ4/x0HUXU5F/cutf%2D…> Cell Press <http://view6.workcast.net/register?pak=5852298848973576&referrer=email> <http://view6.workcast.net/register?pak=5852298848973576&referrer=email> This email has been sent from Cell Press, a division of Elsevier Inc, 600 Technology Square, 5th Floor, Cambridge, MA 02139, USA. If you no longer wish to receive messages of this type, please click here <http://mail.cell-press.com/go.asp?/bECE001/mAFJ954F/q2FJR54F/uE73L71F/x3DQ9…>. Copyright © 2014 Cell Press/Elsevier Inc. All rights reserved. Cell Press Privacy Policy <http://mail.cell-press.com/go.asp?/bECE001/mAFJ954F/qKX1954F/uE73L71F/x3DQ9…>

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[FILM-Users 00458] FW: On Demand webcast: Real time imaging of cells with Super Resolution Microscopy.
by Martin Spitaler 27 Jan '14

27 Jan '14

nature.com webcasts <http://links.ealert.nature.com/ctt?kn=4&ms=NDQ5MjU4MTAS1&r=MTc3MDE3NTE1MQS2…> Custom webcast: sponsor retains sole responsibility for content *The latest innovations **in real-time imaging of cells with Super Resolution Microscopy ** A discussion into new technical innovations for Super Resolution Microscopy** Now available On Demand * ** Speakers: *Dr Jennifer Lippincott-Schwartz* NIH Distinguished Investigator. *Dr Orla Hanrahan* Andor Technology. *Dr Susan Cox* King's College, London. *Register for FREE <http://links.ealert.nature.com/ctt?kn=26&ms=NDQ5MjU4MTAS1&r=MTc3MDE3NTE1MQS…>* Due to the diffraction limit, standard light microscopes can at best resolve features which are around 200nm apart, meaning that they are unsuited to many biological applications. In this webcast, three expert speakers will discuss super resolution microscopy, a rapidly developing field that is breaking the diffraction limit to enable the close examination of finely detailed biological samples. The current focus of the Super Resolution Microscopy community is to increase the utility of existing localisation microscopy techniques such as photo-activated localization microscopy (PALM) and stochastic optical reconstruction microscopy (STORM) – in these methods, fluorophores within a sample emit low levels of light, which is imaged many thousands of times. This creates a single composite image with resolution at the true molecular length scale. However, this process is slow, meaning that these techniques are not best suited to real-time imaging of cells and tissues. Our first speaker, Dr Jennifer Lippincott-Schwartz, will discuss research currently underway using genetically-encoded photoactivatable proteins (PA-FPs) for superresolution imaging which has dramatically expanded the study of cellular organization, function and dynamics. Our second speaker, Dr Susan Cox will go into some detail on the algorithms which facilitate high resolution imaging of live cell dynamics at the nanoscale and lastly Dr Orla Hanrahan will discuss fast and sensitive detector technologies which are suitable for the low light intensities of the single molecule techniques and the speed required for live cell super resolution applications. *Register for FREE <http://links.ealert.nature.com/ctt?kn=26&ms=NDQ5MjU4MTAS1&r=MTc3MDE3NTE1MQS…> Browse through our webcasts <http://links.ealert.nature.com/ctt?kn=4&ms=NDQ5MjU4MTAS1&r=MTc3MDE3NTE1MQS2…> #MSCwebcasts * Sponsor Banner <http://links.ealert.nature.com/ctt?kn=5&ms=NDQ5MjU4MTAS1&r=MTc3MDE3NTE1MQS2…> Sponsor Banner Macmillan <http://links.ealert.nature.com/ctt?kn=2&ms=NDQ5MjU4MTAS1&r=MTc3MDE3NTE1MQS2…> Share:Google+ <http://links.ealert.nature.com/ctt?kn=12&ms=NDQ5MjU4MTAS1&r=MTc3MDE3NTE1MQS…> <http://links.ealert.nature.com/s2s?m=44925810&r=MTc3MDE3NTE1MQS2&rj=MjMwMjE…> Facebook <http://links.ealert.nature.com/s2s?m=44925810&r=MTc3MDE3NTE1MQS2&rj=MjMwMjE…> <http://links.ealert.nature.com/s2s?m=44925810&r=MTc3MDE3NTE1MQS2&rj=MjMwMjE…> LinkedIn <http://links.ealert.nature.com/s2s?m=44925810&r=MTc3MDE3NTE1MQS2&rj=MjMwMjE…> <http://links.ealert.nature.com/s2s?m=44925810&r=MTc3MDE3NTE1MQS2&rj=MjMwMjE…> Reddit <http://links.ealert.nature.com/s2s?m=44925810&r=MTc3MDE3NTE1MQS2&rj=MjMwMjE…> <http://links.ealert.nature.com/s2s?m=44925810&r=MTc3MDE3NTE1MQS2&rj=MjMwMjE…> StumbleUpon <http://links.ealert.nature.com/s2s?m=44925810&r=MTc3MDE3NTE1MQS2&rj=MjMwMjE…> <http://links.ealert.nature.com/s2s?m=44925810&r=MTc3MDE3NTE1MQS2&rj=MjMwMjE…> Twitter <http://links.ealert.nature.com/s2s?m=44925810&r=MTc3MDE3NTE1MQS2&rj=MjMwMjE…> <http://links.ealert.nature.com/s2s?m=44925810&r=MTc3MDE3NTE1MQS2&rj=MjMwMjE…> Digg <http://links.ealert.nature.com/s2s?m=44925810&r=MTc3MDE3NTE1MQS2&rj=MjMwMjE…> As a registered user of Nature Publishing Group's Web sites, our database indicates that you have opted-in to receive third-party email. 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[FILM-Users 00457] Fwd: Lecture at the RI
by Martin Spitaler 27 Jan '14

27 Jan '14

...forwarded for your information; for those new to Imperial College, Dan was a co-founder of FILM and main user in the first few years, but apart from microscopy he has also written a book about the topic he is going to present; should be fun Martin -------- Original Message -------- Subject: Lecture at the RI Date: Fri, 24 Jan 2014 14:34:02 +0000 From: Davis, Daniel M <d.davis(a)imperial.ac.uk> To: Spitaler, Martin <m.spitaler(a)imperial.ac.uk> Hi Martin, Hope all's well. If easy and appropriate, would you perhaps circulate to the imaging community that I'm doing a lecture in London soon, which may be of interest? Details are here: http://www.rigb.org/whats-on/events-2014/march/public-the-compatibility-gene Thanks, Dan Daniel M. Davis Professor of Immunology Director of Research Manchester Collaborative Center for Inflammation Research CTF Building 46 Grafton Street Manchester M13 9NT Tel: 0161 275 5019 BOOK: http://t.co/A0A9810w

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[FILM-Users 00456] Fwd: Oxford Imaging Symposium
by Martin Spitaler 24 Jan '14

24 Jan '14

...forwarded for your information; by the way, plenty more meetings and courses are on the FILM website under LINKS > MEETINGS, COURSES, COMPETITIONS <http://www3.imperial.ac.uk/imagingfacility/links/events>, Martin attachment: <https://workspace.imperial.ac.uk/imagingfacility/Public/_attachment-140124.…> -------- Original Message -------- Subject: Oxford Imaging Symposium Date: Wed, 22 Jan 2014 06:34:47 -0400 From: Gareth Rogers <g.rogers(a)andor.com> Reply-To: Gareth Rogers <g.rogers(a)andor.com> To: <m.spitaler(a)imperial.ac.uk> Oxford Imaging Symposium Hello Martin, Andor Technology invites you to the Oxford Imaging Symposium. The main focus areas are; super-resolution microscopy, light-sheet microscopy and spinning disk confocal microscopy. We are proud to announce that Ernst Stelzer will give the key note lecture at this meeting titled ‘Shifting the paradigm in modern light microscopy Light Sheet-based Fluorescence Microscopy (LSFM, SPIM, DSLM)’. Please see attached a flyer for the complete list of speakers. The event will be held at St. John’s College, University of Oxford on Monday 10^th March 2014. If you wish to attend this workshop, please kindly click on this link to register, andor.com/stjohns. <http://www.msgapp.com/c.aspx?l=CHZD0962310000047190YYY0AD> A copy of the programme will be sent to registered participants shortly. We look forward to seeing you in Oxford. Kind regards, Gareth Rogers Bsc (Hons), PhD Garreth Sloan Research Sales Engineer EMEA Sales Manager *Andor Technology plc* *Andor Technology plc* *discover* new ways of seeing *discover* new ways of seeing *Mobile:* +44 (0) 7795 103 162 *Tel:* 028 9023 7126 (ext 2813) g.rogers(a)andor.comcom <mailto:g.rogers@andor.com> g.sloan(a)andor.com <mailto:g.sloan@andor.com> Address: 7 Millennium Way, BT127AL Springfield Road, Belfast, UK I aim to only send very relevant emails at an acceptable frequency. Please unsubscribe <http://www.msgapp.com/c.aspx?l=CHZD0962310000047190YYY3AD> if you find that this is not the case. If you found this email useful and have a colleague or friend for whom it may also be relevant use this simple link to assist with forwarding <http://www.msgapp.com/c.aspx?l=CHZD0962310000047190YYY4AD>.

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