June 2012 - FILM-Users - imperial.ac.uk

[FILM-Users 00233] TODAY: FILM Microscopy Day 2012
by Martin Spitaler 29 Jun '12

29 Jun '12

Dear microscopists, just a reminder TODAY is the FILM MICROSCOPY DAY - EVERYBODY IS WELCOME (you don't need to be a FILM user) There will also be a short overview of FILM for new people, and WHAT'S NEW for existing users, e.g. the new microscopes in Hammersmith, new Huygens deconvolution software (including confocal and multiphoton modules), just before the match maker session >>AGENDA<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< 10.00- 10.10 Welcome by Prof Tony Magee, head of FILM GENERAL MICROSCOPY TECHNIQUES 10.10-10.55 Basics of microscopy - magnification, properties of light, resolution, transmitted light contrast (PH, DIC, darkfield), epifluorescence, confocal microscopy (Martin Spitaler, FILM) 10.55-11.40 Live imaging techniques - Multiphoton, live imaging and second harmonic generation (SHG) (Mark Scott, FILM) 11.40-12.00 coffee & tea break 12.000-13.00 MICROSCOPY MATCHMAKER: FILM users giving a snapshot of their work in 2-minute presentations Introduction: FILM overview, what's new in FILM (Martin Spitaler) # David Hodson: tba # Katrin Kronenberger: Optimisation of whole mount imaging # Malak Al-Yamani & Martina Lundberg: Imaging the inside wall of the mouse aortic arch # Mirella Koleva: Study of Sonic hedgehog multimerisation # Nickolai Vysokov: Role of Latrophilin-Lasso trans-synaptic intreaction # Paulina Ciepla: tba # Sophie Pageon: Imaging the nanoscale organisation of KIR2DL1 using PALM 13.00-14.15 Poster session with sandwich lunch, ground floor (outside cafeteria) (kindly sponsored by CARL ZEISS MICROSCOPY) SPECIAL TECHNIQUES 14.15-15.00 Fluorophores as sensors - colocalisation, fluorescence lifetime (FLIM) and resonant energy transfer (FRET) (Valentina Caorsi, NHLI) 15.00-15.45 Super-resolution microscopy - TIRF, PALM, STORM (Steve Rothery, FILM) 15.45-16.05 coffee & tea break UNDERSTANDING IMAGES 16.05-16.30 Image data: data formats, pixel and metadata, image visualisation and presentation, OMERO database (Martin Spitaler, FILM) 16.30-17.35 Image analysis: noise removal, deconvolution, segmentation, tracking, registration (Luis Pizarro, Computing) 17.35-17.40 Wrap-up, feedback, outlook For more and up-to-date information, please check <http://www3.imperial.ac.uk/portal/page/portallive/imagingfacility/news>. See you all there, good imaging, Martin ############################################# -- Martin Spitaler, PhD FILM - Facility for Imaging by Light Microscopy - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk Website: http://imperial.ac.uk/imagingfacility

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[FILM-Users 00232] COMMERCIAL Fwd: Badrilla antibodies
by Martin Spitaler 27 Jun '12

27 Jun '12

...forwarded for your information; By the way, FILM users: I'm careful not to flood your inboxes with commercial emails, but thought it could be interesting, and ok if it's clearly stated in the subject; please let me know what you think about forwarding special offers and other commercial information, Martin -------- Original Message -------- Subject: A request for some help Date: Wed, 13 Jun 2012 04:44:05 -0700 From: Gavin Allsop <g.allsop(a)badrilla.com> To: <m.spitaler(a)IMPERIAL.AC.UK> Dear Martin. I hope that you are well. I wonder if you could help? I completed my PhD in 2010 at the University of Leeds in the School of Molecular and Cellular Biology, this project was largely based around microscopy and molecular biology. I now work for Badrilla Ltd <http://badrilla.com/>, a UOL spin-off company whose goal is to improve the supply of good quality antibodies for university research. In my experience as a researcher, this is long overdue. We produce well validated antibodies, presented honestly in real-world experimental settings. I hope to make a positive contribution to the industry. Here’s the request for help. Please would you circulate the technical details of my first wave of products (c-Myc <http://badrilla.com/gfp-polyclonal-5.html> | GFP <http://badrilla.com/gfp-polyclonal-5.html> | mCherry <http://badrilla.com/pmcherry-5.html>) to your research colleagues and inform them that further antibodies to beta-tubulin <http://dl.dropbox.com/u/41917315/Data%20Sheets/A010-bTub.pdf>, GST <http://dl.dropbox.com/u/41917315/Data%20Sheets/A010-GST.pdf> and MBP <http://dl.dropbox.com/u/41917315/Data%20Sheets/A010-MBP.pdf> will be released in the coming months. We hope to become a trusted and more affordable alternative to the industries giants for UK universities. With best regards Gavin -------------- Dr Gavin Allsop Badrilla Ltd Leeds Innovation Centre 103 Clarendon Road Leeds LS2 9DF United Kingdom -------------- http://badrilla.com Twitter <http://twitter.com/@BadrillaLtd>Facebook <http://facebook.com/BadrillaLtd>

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[FILM-Users 00231] reminder - THIS FRIDAY: FILM Microscopy Day 2012
by Martin Spitaler 25 Jun '12

25 Jun '12

Just a reminder about THIS FRIDAY'S FILM MICROSCOPY DAY. And be aware, EVERYBODY IS WELCOME (you don't need to be a FILM user, feel free to forward this to anybody else), but if to help with catering, please register with Ruth A O'Donnell <r.odonnell(a)imperial.ac.uk> if you haven't done so yet. And if interested, one or two slots for the Matchmaker presentation or poster session are also still available - be quick. Martin -------- Original Message -------- Subject: FILM Microscopy Day 2012 Date: Mon, 11 Jun 2012 14:25:36 +0100 From: Martin Spitaler <m.spitaler(a)imperial.ac.uk> To: FILM-Users mailing list <film-users(a)imperial.ac.uk> CC: Ruth A O'Donnell <r.odonnell(a)imperial.ac.uk> Dear microscopists and supervisors, the FILM MICROSCOPY DAY 2012 is now only just over two 2 weeks - so if you haven't done so yet, please register NOW! With all the extra bank holidays you might have forgotten about it, but you need to register your slot on the Matchmaking Session, your chance to get new ideas, start networking, find solutions to your microscopy problems. Or if you really can't make that, at least your slot in the poster session, or at the very least to make sure there is enough catering! SUPERVISORS and PIs: I seems that students and postdocs need a bit of a push to take part in our Matchmaking Session. We do believe that this is an excellent opportunity for them to present the work they are doing (advertise your lab), find networking opportunities and on the side practice their presentation skills. So please make sure they don't miss out and convince them to register and take it up. For details, see below. THE EXTENDED REGISTRATION DEADLINE IS FRIDAY 15 JUNE 2012 Good imaging, Martin ======================================================== Dear microscopists, on FRIDAY 29 JUNE we will have the FILM MICROSCOPY DAY 2012 - as in previous years, a day full of talks on all aspects of light microscopy, from the very basics to very advanced technologies (in vivo imaging, super-resolution, non-linear techniques). The presentations will be by FILM staff (Steve, Mark and myself) supported by two experts in their field, VALENTINA CAORSI (NHLI) on non-linear fluorescence techniques (fluorescence lifetime imaging and its various applications) and LUIS PIZARRO (COMPUTING) on image analysis. We also have a special novelty: To make people aware what's going on around them and stimulate new collaborations, we will have a MICROSCOPY MATCH MAKER, where you FILM users are all invited to present your exciting research projects in a short snapshot oral presentation and / or a poster. Oral presentations are strictly limited to a MAXIMUM OF 2 MINUTES / 2 POWERPOINT SLIDES - longer discussions will be possible at the posters over lunchtime. An excellent opportunity to initiate new collaborations, don't miss out - PIs, please encourage your students and postdocs to participate. Please REGISTER BY FRIDAY 15 JUNE 2012 by email to Ruth O'Donnell <r.odonnell(a)imperial.ac.uk>, including your MATCHMAKER OPTIONS (presentation, poster, none). >>PROGRAMME<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< 10.00- 10.10 Welcome by Prof Tony Magee, head of FILM GENERAL MICROSCOPY TECHNIQUES 10.10-10.55 Basics of microscopy - magnification, properties of light, resolution, transmitted light contrast (PH, DIC, darkfield), epifluorescence, confocal microscopy (Martin Spitaler, FILM) 10.55-11.40 Live imaging techniques - Multiphoton, live imaging and second harmonic generation (SHG) (Mark Scott, FILM) 11.40-12.00 coffee & tea break 12.000-13.00 MICROSCOPY MATCHMAKER: FILM users giving a snapshot of their work in 2-minute presentations 13.00-14.15 Poster session with sandwich lunch (kindly sponsored by CARL ZEISS MICROSCOPY) SPECIAL TECHNIQUES 14.15-15.00 Fluorophores as sensors - colocalisation, fluorescence lifetime (FLIM) and resonant energy transfer (FRET) (Valentina Caorsi, NHLI) 15.00-15.45 Super-resolution microscopy - TIRF, PALM, STORM (Steve Rothery, FILM) 15.45-16.05 coffee & tea break UNDERSTANDING IMAGES 16.05-16.50 Understanding and visualisation of image data: data formats, pixel and metadata, image visualisation and presentation, OMERO database (Martin Spitaler, FILM) 16.50-17.35 Image analysis: noise removal, deconvolution, segmentation, tracking, registration (Luis Pizarro, Computing) 17.35-17.40 Wrap-up, feedback, outlook For more and up-to-date information, please check <http://www3.imperial.ac.uk/portal/page/portallive/imagingfacility/news>. A flyer is also available <https://workspace.imperial.ac.uk/imagingfacility/Public/FILM%20Microcopy%20…> See you all there. Good imaging, Martin -- ------------------------------------------------------------------------ *Martin Spitaler, PhD* *FILM - Facility for Imaging by Light Microscopy* - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk <mailto:m.spitaler@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

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[FILM-Users 00230] Fwd: Beautiful Science Exhibition is here!!!
by Martin Spitaler 21 Jun '12

21 Jun '12

...forwarded, for your information: A very interesting event coming up, grown out of a postdoc project involving several FILM users to combine science and art, Martin -------- Original Message -------- Subject: Beautiful Science Exhibition is here!!! Date: Thu, 21 Jun 2012 12:03:21 +0100 From: Archanco, Marta <m.archanco(a)imperial.ac.uk> Dear All, Can you please help us distributing the below email to your contacts? We would like to see us advertised everywhere but specially interested in getting it in more art/design media and from any country (we already have a journal from Australia that is writing about us!!!). Any help with this will be very much appreciated. Many thanks for your help and we hope you will all enjoy the exhibition. Kind regards, Marta Archanco Beautiful Science Project Manager /We would like to invite you to join us at an exhibition exploring the relationship between Science and Art./ /cid:1DFA968E-08E1-4554-8793-231630067D08/ *26th June - 2nd July, 1- 6 pm* *The Brick Lane Gallery (Annexe)* 196 Brick Lane, E1 6SA London *Is there beauty in raw scientific data?* A group of Imperial College scientists think so -- and have collaborated with artists in an experiment to translate and communicate this beauty to an audience of art-goers. The relationship between the raw data and artistic interpretation varies across a spectrum from literal and direct engagement to highly abstract, evocative works that bear only the echo of scientific inspiration. The scientific data on display encompasses photography of world invisible to the naked eye and the graphs, charts and records that are part and parcel of scientific practice. The artworks range across media, encompassing film, photography, painting and design. More details: beautiful.science(a)imperial.ac.uk <mailto:beautiful.science@imperial.ac.uk> http://www.beautifulscience.info/ http://www.facebook.com/beausci <http://www.beautifulscience.info/> <http://www.beautifulscience.info/> <http://www.beautifulscience.info/> *Supported by:* Imperial College, WellCome Trust, Kingston University, ELGA, KIMTECH

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[FILM-Users 00229] clarification: registrations for FILM Microscopy Day 2012
by Martin Spitaler 20 Jun '12

20 Jun '12

Dear microscopists, I have been told by some users that there was a confusion about the participation in the FILM MICROSCOPY DAY: Just to clarify, EVERYBODY IS WELCOME TO ATTEND, whether you are presenting your own work or just want to sit in and watch the lectures, Matchmaker session and posters. But because we need to know the number of people attending for catering purposes, please still DO REGISTER! As soon as possible. Otherwise you might not get a coffee or sandwich! Good imaging, Martin -------- Original Message -------- Subject: FILM Microscopy Day 2012 Date: Mon, 11 Jun 2012 14:25:36 +0100 From: Martin Spitaler <m.spitaler(a)imperial.ac.uk> To: FILM-Users mailing list <film-users(a)imperial.ac.uk> CC: Ruth A O'Donnell <r.odonnell(a)imperial.ac.uk> Dear microscopists and supervisors, the FILM MICROSCOPY DAY 2012 is now only just over two 2 weeks - so if you haven't done so yet, please register NOW! With all the extra bank holidays you might have forgotten about it, but you need to register your slot on the Matchmaking Session, your chance to get new ideas, start networking, find solutions to your microscopy problems. Or if you really can't make that, at least your slot in the poster session, or at the very least to make sure there is enough catering! SUPERVISORS and PIs: I seems that students and postdocs need a bit of a push to take part in our Matchmaking Session. We do believe that this is an excellent opportunity for them to present the work they are doing (advertise your lab), find networking opportunities and on the side practice their presentation skills. So please make sure they don't miss out and convince them to register and take it up. For details, see below. THE EXTENDED REGISTRATION DEADLINE IS FRIDAY 15 JUNE 2012 Good imaging, Martin ======================================================== Dear microscopists, on FRIDAY 29 JUNE we will have the FILM MICROSCOPY DAY 2012 - as in previous years, a day full of talks on all aspects of light microscopy, from the very basics to very advanced technologies (in vivo imaging, super-resolution, non-linear techniques). The presentations will be by FILM staff (Steve, Mark and myself) supported by two experts in their field, VALENTINA CAORSI (NHLI) on non-linear fluorescence techniques (fluorescence lifetime imaging and its various applications) and LUIS PIZARRO (COMPUTING) on image analysis. We also have a special novelty: To make people aware what's going on around them and stimulate new collaborations, we will have a MICROSCOPY MATCH MAKER, where you FILM users are all invited to present your exciting research projects in a short snapshot oral presentation and / or a poster. Oral presentations are strictly limited to a MAXIMUM OF 2 MINUTES / 2 POWERPOINT SLIDES - longer discussions will be possible at the posters over lunchtime. An excellent opportunity to initiate new collaborations, don't miss out - PIs, please encourage your students and postdocs to participate. Please REGISTER BY FRIDAY 15 JUNE 2012 by email to Ruth O'Donnell <r.odonnell(a)imperial.ac.uk>, including your MATCHMAKER OPTIONS (presentation, poster, none). >>PROGRAMME<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< 10.00- 10.10 Welcome by Prof Tony Magee, head of FILM GENERAL MICROSCOPY TECHNIQUES 10.10-10.55 Basics of microscopy - magnification, properties of light, resolution, transmitted light contrast (PH, DIC, darkfield), epifluorescence, confocal microscopy (Martin Spitaler, FILM) 10.55-11.40 Live imaging techniques - Multiphoton, live imaging and second harmonic generation (SHG) (Mark Scott, FILM) 11.40-12.00 coffee & tea break 12.000-13.00 MICROSCOPY MATCHMAKER: FILM users giving a snapshot of their work in 2-minute presentations 13.00-14.15 Poster session with sandwich lunch (kindly sponsored by CARL ZEISS MICROSCOPY) SPECIAL TECHNIQUES 14.15-15.00 Fluorophores as sensors - colocalisation, fluorescence lifetime (FLIM) and resonant energy transfer (FRET) (Valentina Caorsi, NHLI) 15.00-15.45 Super-resolution microscopy - TIRF, PALM, STORM (Steve Rothery, FILM) 15.45-16.05 coffee & tea break UNDERSTANDING IMAGES 16.05-16.50 Understanding and visualisation of image data: data formats, pixel and metadata, image visualisation and presentation, OMERO database (Martin Spitaler, FILM) 16.50-17.35 Image analysis: noise removal, deconvolution, segmentation, tracking, registration (Luis Pizarro, Computing) 17.35-17.40 Wrap-up, feedback, outlook For more and up-to-date information, please check <http://www3.imperial.ac.uk/portal/page/portallive/imagingfacility/news>. A flyer is also available <https://workspace.imperial.ac.uk/imagingfacility/Public/FILM%20Microcopy%20…> See you all there. Good imaging, Martin -- ------------------------------------------------------------------------ *Martin Spitaler, PhD* *FILM - Facility for Imaging by Light Microscopy* - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk <mailto:m.spitaler@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

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[FILM-Users 00228] Fwd: Beautiful Science Open Discussion - Please register! - All welcome
by Martin Spitaler 18 Jun '12

18 Jun '12

Dear microscopists, several of you are already involved in this and know about the BEAUTIFUL SCIENCE initiative, an initiative started by some Imperial College postdocs, and now involving a range of scientists and artists. As microscopists, certainly all of you will have some interest in the beautiful aspects of science. So please see below the information about the exhibition and the DISCUSSION TODAY. Sorry for the late notice, last week was just completely taken up by the opening of our Hammersmith Campus branch and the Facility Showcase. I hope many of you can still make it, hopefully see you there. Good - and beautiful - imaging, Martin -------- Original Message -------- Subject: Beautiful Science Open Discussion - Please register! - All welcome Date: Wed, 13 Jun 2012 16:37:52 +0100 From: Beautiful Science <beautiful.science(a)imperial.ac.uk> Hello all! The Beautiful Science Live Discussion is just a few days away. It will be very exciting! Come along and invite anyone that might be interested!Please follow the registration link below. Best wishes, Beautiful Science Management Team http://www.beautifulscience.info/ *Science and Art: a long way together?** **/They might not be as distant as one imagines /*/ /*/what?/* A panel discussion exploring the relationship between science and art / /*/when?/*/ /Monday 18th June - Drinks reception at 6.30pm - Discussion at 7.00pm / /*/where?/*/ /Lecture Theatre G34 – SAF Building, Imperial College London / /*/who? /**Chair - Stephen Webster *Director of Science Communication Group at Imperial College London* *Catherine McDermott** Professor of Curating Science, School of Communication Design, Kingston College* *Erica Seccombe **Visual Artist and PhD Candidate at Australian National University *Anne Odling-Smee*Director of O-SB Design and Lecturer at Central Saint Martins College of Art and Design * *Fernando Abaitua** Senior Postdoctoral Researcher, Division of Infectious Diseases at Imperial College London */how?/* Attendance is *free *but please *register here* <http://beausci.eventbrite.com/>: <http://beausci.eventbrite.com/> * * For the full biographies of the panelist please visit: http://www.beautifulscience.info/ You can also join us on Facebook: http://www.facebook.com/beausci ================================================================== Text version: Live discussion at Imperial College June 18th, 6:30 pm SAF building, Lecture room G34, Imperial College London, South Kensington Campus Please help us planning and register here for free. Panel (Detailed bios): Stephen Webster (Chair), Director of Science Communication Group, Imperial College London Catherine McDermott, Professor of Curating Science, School of Communication Design, Faculty of Art, Design and Architecture, Kingston College Erica Seccombe, Visual artist and PhD Candidate at Australian National University Anne Odling-Smee, O-SB Design Director and Lecturer at Central Saint Martins College of Art and Design Fernando Abaitua, Senior Postdoctoral Researcher, Division of Infectious Diseases at Imperial College London Exhibition exploring Art & Science at The Brick Lane Gallery (Annexe) 26th June – 2nd July 2012, 1 – 6 pm Scientific discoveries are portrayed in many different forms in the media, but the work scientists carry out every day to produce the key building blocks to discovery is rarely shown. We are a group of biomedical scientists who are working with artists so that we can share our unseen scientific work with you. Click on our images below to follow the progress of the artists’ projects as they work with us to explore and interpret our scientific data.

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[FILM-Users 00227] Fwd: TODAY: CITC symposium and Facilities Showcase - Friday June 15th
by Martin Spitaler 15 Jun '12

15 Jun '12

...forwarded for your information; of particular interest could be the FACILITY SHOWCASE from 12 to 3pm, Martin -------- Original Message -------- Subject: Programme for CITC symposium and Facilities Showcase - Friday June 15th Date: Thu, 14 Jun 2012 17:54:44 +0100 From: Sharp, Emma L <e.sharp(a)imperial.ac.uk> Dear All, Please find attached the finalised programme for the Chemistry In The Clinic Symposium and Facilities Showcase taking place tomorrow from 9:30-6pm, G34 and 119-122, Level 1, Sir Alexander Fleming Building, South Kensington Campus. We look forward to seeing you at this event. Best wishes, Emma http://www3.imperial.ac.uk/newsandeventspggrp/imperialcollege/naturalscienc… <http://www3.imperial.ac.uk/newsandeventspggrp/imperialcollege/naturalscienc…> -------------------------- Dr Emma L Sharp Project Manager Faculty of Natural Sciences Level 3, Faculty Building Imperial College London Exhibition Road, London SW7 2AZ 020 759 41425 07872 850031 e.sharp(a)imperial.ac.uk <mailto:e.sharp@imperial.ac.uk>

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[FILM-Users 00226] New FILM microscopes in Hammersmith: OPENING & DEMONSTRATION DAY Thu 14 June 2012
by Martin Spitaler 12 Jun '12

12 Jun '12

Dear microscopists, this is a reminder that this week on THURSDAY 14 JUNE 2012 we have the OPENING & DEMONSTRATION DAY of our new FILM microscopes in the new L-Block building on Hammersmith campus. With generous funding from BHF to NHLI, two top-class microscopes have been purchased, a Zeiss LSM-780 confocal with GaAsP metadetector and an AxioObserver with LED illumination for fast multi-channel acquisition, a Hamamatsu Flash 4.0 high-resolution / high-sensitivity sCMOS camera and a Vivatome for fast 3D acquisition. Details of the microscopes will soon be available on our website and in our WIKI, I'll keep you posted. Installation and testing is now done, from next week the microscopes will be available to users in NHLI as well as other Imperial College users (time permitting). The microscopes will be part of FILM, so you can expect the same training and support as you are used to from the main FILM site on the South Kensington campus. The new FILM branch will be started with an OPENING AND DEMONSTRATION DAY this THURSDAY 14 JUNE 2012 (with further hands-on demonstrations on Friday) THURSDAY 14 JUNE MORNING SESSION (Wolfson Building LT2): Presentations 10.00-10.10 (Prof Tony Magee, head of FILM): Welcome and opening of the L-Block branch of FILM 10.10-10.20 (Martin Spitaler, FILM): Overview and specifications of the new microscopes 10.20-11.20 (David Crook, Zeiss): Functions and applications of the widefield microscope setup (AxioObserver with Vivatome, Colibri LED illumination and Hamamatsu Flash 4.0 camera) # hardware specifications # software # high-speed acquisition (fast LED switching, fast camera options) # automated tiled, multi-position and multi-well acquisition # 3D acquisition with the Vivatome 11.20-11.30 coffee break 11.30-13.00 (Graham Brown, Zeiss): Functions and applications of the confocal microscope setup (LSM-780 Meta) # hardware specifications # confocal-specific software functions # setup of fluorophor-specific light paths, PMT versus Meta-GaAsp detectors # spectral acquisition with the Meta detector and spectral unmixing 13.00-14.00 lunch break AFTERNOON SESSION (Microscope Room L-Block 314): 14.00-17.00: hands-on session for users with their own sample (advance booking) FRIDAY: Further hands-on session for users with their own sample, according to demand If you haven't done so yet, please book your own personal introduction with your own samples (2-hour time slots) via email to Stephen M Rothery <s.rothery(a)imperial.ac.uk> (this introduction will NOT replace your own personal training session, which will still be provided later). Presentations on Thursday morning will be open to everybody, please feel free to spread the word and forward this email. Good imaging, Martin -- ------------------------------------------------------------------------ *Martin Spitaler, PhD* *FILM - Facility for Imaging by Light Microscopy* - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk <mailto:m.spitaler@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

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[FILM-Users 00225] Fwd: FW: post-doc position at EMBL Monterotondo
by Martin Spitaler 12 Jun '12

12 Jun '12

...and another microscopy-related job opportunity below, and again in an interesting place - the former (intensive) FILM user and PhD student Giulia Bolasco is running the imaging facility there now. Martin PS: because the Mailman system doesn't support attachments, I've copied you the information in as plain text below -------- Original Message -------- Subject: FW: post-doc position at EMBL Monterotondo Date: Sat, 9 Jun 2012 19:28:30 +0100 From: Lo Celso, Cristina <c.lo-celso(a)imperial.ac.uk> To: david.g.kent(a)gmail.com <david.g.kent(a)gmail.com>, Hawkins, Edwin <e.hawkins(a)imperial.ac.uk>, Davis, Daniel M <d.davis(a)imperial.ac.uk>, dominique.bonnet(a)cancer.org.uk <dominique.bonnet(a)cancer.org.uk>, Spitaler, Martin <m.spitaler(a)imperial.ac.uk>, kbj22(a)cam.ac.uk <kbj22(a)cam.ac.uk>, michaela.frye(a)cancer.org.uk <michaela.frye(a)cancer.org.uk>, k.braun(a)qmul.ac.uk <k.braun(a)qmul.ac.uk>, Giovanna Lalli <giovanna.lalli(a)kcl.ac.uk>, Gounaris, Kleoniki <k.gounaris(a)imperial.ac.uk>, Rashidi, Narges M <n.rashidi(a)imperial.ac.uk>, Ferraro, Francesca <FFERRARO(a)PARTNERS.ORG> Dear all, attached is the advert for a postdoc position to work on HSC specification during embryonic development, based in Monterotondo (EMBL). Please feel free to pass it to anybody you may know that could be interested! Thank you and best wishes Cristina 24 June 2012 Reference number: MR_00011 Job Description The European Molecular Biology Laboratory (EMBL) is one of the highest ranked scientific research organizations in the world. The Headquarters Laboratory is located in Heidelberg (Germany) and the outstations are in Grenoble (France), Hamburg (Germany), Hinxton (UK) and Monterotondo (Italy). We are looking for a very qualified and motivated individual to fill a post-doctoral researcher position in the Lancrin group working on the cellular origin of blood cells during mouse embryogenesis at EMBL Monterotondo, Italy. Using genetic approaches coupled with embryonic stem (ES) cell differentiation and generation of mouse model, we plan to identify and study the genes responsible for the generation of the first blood progenitors during embryonic life. Our aim is to gain a better understanding of the cell fate decisions leading to the production of the first hematopoietic cells and to use this knowledge to improve methods of blood cells generation from ES Cell or induced Pluripotent Stem Cell. Qualifications and Experience Applicants should hold a PhD degree in developmental biology, molecular biology, or mouse biology. Candidates with experience in ES cell culture, flow cytometry and microscopy will be preferred. An interest in hematopoiesis is highly desirable. The candidate should be able to demonstrate the capacity to run a project independently. Fluency in English, good communication and interpersonal skills, excellent organizational skills and the ability to work as part of an international team are required. The candidate will benefit of the state of the art EMBL core facilities as well as its stimulating and interdisciplinary environment. For informal inquiry please contact Dr Christophe Lancrin: christophe.lancrin(a)embl.it. Application Instructions Please apply online through www.embl.org/jobs Additional Information EMBL is an inclusive, equal opportunity employer offering attractive conditions and benefits appropriate to an international research organization. ------------------------------------------------------------------------ *From:* Christophe Lancrin [christophe.lancrin(a)embl.it] *Sent:* 08 June 2012 15:22 *To:* Lo Celso, Cristina *Subject:* post-doc position at EMBL Monterotondo Dear Cristina, I hope you had a nice week. I have a post-doc position to fill in my lab. Would you like to pass the information around for me? It would be very nice. The advertisement is attached. Please let me know. Best, Christophe -- Christophe Lancrin, PhD Group Leader EMBL Monterotondo Mouse Biology Unit Campus Adriano Buzzati-Traverso Via Ramarini 32 00015 Monterotondo, Italy Tel: +39 06 90091 218 christophe.lancrin(a)embl.it http://www.embl.it/ http://www.lancrinlaboratory.com/

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[FILM-Users 00224] Fwd: OMERO developer post Pasteur Paris
by Martin Spitaler 12 Jun '12

12 Jun '12

...forwarded for your information; this should be a very interesting post, in an exciting microscopy environment, with lots of new developments going on, and Spencer is very nice person to work with; plus, the added bonus of reputedly the best canteen of Paris (and that's not by Imperial standards, I can confirm it's excellent), Martin -------- Original Message -------- Subject: OMERO developer post Pasteur Paris Date: Thu, 7 Jun 2012 08:45:52 +0200 From: Spencer Shorte <sshorte(a)pasteur.fr> To: Martin Spitaler <m.spitaler(a)imperial.ac.uk> Software developer post at the Imagopole facilities of the Institut Pasteur Paris The Imagopole (http://www.imagopole.org) is a large multi-disciplinary centre for functional analyses of biological systems using optical and electron microscope imaging methods. It serves primarily researchers working on infection, microbiology and immunology at the Institut Pasteur Paris, but is also open at national and international levels for external research needs. With around fifty associate research and engineering staff, rendering advanced technologies for life sciences research to over five hundred users, we have special need for excellence in imaging informatics, and currently are seeking a an experienced software developer to join our team, for an appointment of three years. Specifically, the candidate will join the Imagopole in the context of a newly funded program (Wellcome Trust) aimed to develop OME/OMERO (OME) image database architecture for the benefit of a consortium of partners, including the Institut Pasteur campus. Towards these ends the Open Microscopy Environment Consortium (OME) is in an exciting, well-funded initiative aiming to leverage great functionality for image db management through a community effort for improved and updated Bio-Formats; integration with ImageJ2, CellProfiler, and KNIME. Downstream there are plans for improved support for heterogeneous data, probably via NoSQL; improved data model and ontology support; improved support for distributed compute for large-scale analysis. While OMERO applications are mostly written in Java and Python with some JavaScript and PL/pgSQL the foundations are old-school (PostgreSQL, ZeroC's ICE mixed with a taste of web scale computing; NoSQL, HDF5 and PyTables). So, the candidate must have the technical skills to integrate to this professional team, and the independence to use their skills for local developments in the Institut Pasteur Imagopole. The successful applicant will be based in Paris France, and will interact closely with researchers and software developers based in Scotland, Germany, Italy, and the USA. For more information, see http://openmicroscopy.org. Responsibilities: . Client and server side development; . Rapid prototyping based on new feature requests; . Participation in the entire life cycle of our applications; development, testing and deployment; . Continue development of an existing Java server-based Application Requirements: . Java and/or Python programming experience; . Database-driven application design and data modelling; . Experience with client-server application development; . Experience with SQL; . Excellent object-oriented and aspect-oriented programming skills; . Experience with Java server development; . Experience with Linux, Mac OS X or any proprietary Unix flavour; . Strong collaborative skills with an eye towards efficiency and creativity Expertise in one or more of the following: . C, C++ and/or Python programming experience; . Template design with Django; . Experience developing and deploying Java client-server applications in Linux and/or Windows; . A procedural SQL language (PL/SQL, Transact-SQL, PL/pgSQL, SQL PL); . Oracle or PostgreSQL experience; . ZeroC's ICE; . NoSQL facility; . Experience in an open source development project Interested: Send CV and a letter of intention to: admin-imagopole(a)pasteur.fr (Attention: Pr. Spencer Shorte)

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