Dear microscopists, already the month is nearing its end, so it's time for the next FILM Club. Unfortunately we had to postpone it last time (sorry for those who turned up anyway), but this week it's really happening: Friday 27 Jan 2012, 13.00, SAFB-120 (South Kensington) KIERAN McGOURTY: INHIBITION OF LYSOSOME FUNCTION IN SALMONELLA For this study, Kieran measured the pH inside the cytosol and lysosomes with pH-sensitive dyes. He went to great lengths to measure the pH in a reproducible and quantifiable way. In this FILM Club he will explain how he has managed to calibrate the fluorescence signal, which dyes turned out best for measurement of the acidic lysosomes, and obviously what interesting results he achieved with all this. See you there, good imaging, Martin -- ------------------------------------------------------------------------ *Martin Spitaler, PhD* *FILM - Facility for Imaging by Light Microscopy* - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler@imperial.ac.uk <mailto:m.spitaler@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility