Forwarding this request - can anyone help Chris with his yeast staining? Martin -------- Original Message -------- Date: Mon, 30 Jan 2012 17:19:18 +0000 From: Grice, Christopher <c.grice11@imperial.ac.uk> To: Spitaler, Martin <m.spitaler@imperial.ac.uk> Dear Martin, I was wondering if, for when you image cells using primary and secondary antibodies, you spheroplast the cells (i.e. for yeast), and if so, do you use zymolase? And would you perhaps have a sample I could use? Also I was wondering what mounting media you may use for preparing the finished slides for viewing? Many thanks in advance for any help you can provide, Chris