Just a reminder about THIS FRIDAY'S FILM MICROSCOPY DAY. And be aware, EVERYBODY IS WELCOME (you don't need to be a FILM user, feel free to forward this to anybody else), but if to help with catering, please register with Ruth A O'Donnell <r.odonnell@imperial.ac.uk> if you haven't done so yet. And if interested, one or two slots for the Matchmaker presentation or poster session are also still available - be quick. Martin -------- Original Message -------- Subject: FILM Microscopy Day 2012 Date: Mon, 11 Jun 2012 14:25:36 +0100 From: Martin Spitaler <m.spitaler@imperial.ac.uk> To: FILM-Users mailing list <film-users@imperial.ac.uk> CC: Ruth A O'Donnell <r.odonnell@imperial.ac.uk> Dear microscopists and supervisors, the FILM MICROSCOPY DAY 2012 is now only just over two 2 weeks - so if you haven't done so yet, please register NOW! With all the extra bank holidays you might have forgotten about it, but you need to register your slot on the Matchmaking Session, your chance to get new ideas, start networking, find solutions to your microscopy problems. Or if you really can't make that, at least your slot in the poster session, or at the very least to make sure there is enough catering! SUPERVISORS and PIs: I seems that students and postdocs need a bit of a push to take part in our Matchmaking Session. We do believe that this is an excellent opportunity for them to present the work they are doing (advertise your lab), find networking opportunities and on the side practice their presentation skills. So please make sure they don't miss out and convince them to register and take it up. For details, see below. THE EXTENDED REGISTRATION DEADLINE IS FRIDAY 15 JUNE 2012 Good imaging, Martin ======================================================== Dear microscopists, on FRIDAY 29 JUNE we will have the FILM MICROSCOPY DAY 2012 - as in previous years, a day full of talks on all aspects of light microscopy, from the very basics to very advanced technologies (in vivo imaging, super-resolution, non-linear techniques). The presentations will be by FILM staff (Steve, Mark and myself) supported by two experts in their field, VALENTINA CAORSI (NHLI) on non-linear fluorescence techniques (fluorescence lifetime imaging and its various applications) and LUIS PIZARRO (COMPUTING) on image analysis. We also have a special novelty: To make people aware what's going on around them and stimulate new collaborations, we will have a MICROSCOPY MATCH MAKER, where you FILM users are all invited to present your exciting research projects in a short snapshot oral presentation and / or a poster. Oral presentations are strictly limited to a MAXIMUM OF 2 MINUTES / 2 POWERPOINT SLIDES - longer discussions will be possible at the posters over lunchtime. An excellent opportunity to initiate new collaborations, don't miss out - PIs, please encourage your students and postdocs to participate. Please REGISTER BY FRIDAY 15 JUNE 2012 by email to Ruth O'Donnell <r.odonnell@imperial.ac.uk>, including your MATCHMAKER OPTIONS (presentation, poster, none).

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PROGRAMME<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<

10.00- 10.10 Welcome by Prof Tony Magee, head of FILM GENERAL MICROSCOPY TECHNIQUES 10.10-10.55 Basics of microscopy - magnification, properties of light, resolution, transmitted light contrast (PH, DIC, darkfield), epifluorescence, confocal microscopy (Martin Spitaler, FILM) 10.55-11.40 Live imaging techniques - Multiphoton, live imaging and second harmonic generation (SHG) (Mark Scott, FILM) 11.40-12.00 coffee & tea break 12.000-13.00 MICROSCOPY MATCHMAKER: FILM users giving a snapshot of their work in 2-minute presentations 13.00-14.15 Poster session with sandwich lunch (kindly sponsored by CARL ZEISS MICROSCOPY) SPECIAL TECHNIQUES 14.15-15.00 Fluorophores as sensors - colocalisation, fluorescence lifetime (FLIM) and resonant energy transfer (FRET) (Valentina Caorsi, NHLI) 15.00-15.45 Super-resolution microscopy - TIRF, PALM, STORM (Steve Rothery, FILM) 15.45-16.05 coffee & tea break UNDERSTANDING IMAGES 16.05-16.50 Understanding and visualisation of image data: data formats, pixel and metadata, image visualisation and presentation, OMERO database (Martin Spitaler, FILM) 16.50-17.35 Image analysis: noise removal, deconvolution, segmentation, tracking, registration (Luis Pizarro, Computing) 17.35-17.40 Wrap-up, feedback, outlook For more and up-to-date information, please check <http://www3.imperial.ac.uk/portal/page/portallive/imagingfacility/news>. A flyer is also available <https://workspace.imperial.ac.uk/imagingfacility/Public/FILM%20Microcopy%20Day%202012.pdf> See you all there. Good imaging, Martin -- ------------------------------------------------------------------------ *Martin Spitaler, PhD* *FILM - Facility for Imaging by Light Microscopy* - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler@imperial.ac.uk <mailto:m.spitaler@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility