Dear microscopists, in next week's FILM Club I will give an overview about the application of Definiens Developer XD for the analysis of complex microscopy data in the facility. Definiens Developer XD is a powerful software tool to extract complex information from unstructured image data, way beyond the usual thresholding and filtering approaches biologists like to use. It gives biologists easy access to a large range of advanced image analysis tools, which allows to analyse images in a similar way to visual inspection, handling challenging problems like low contrast, label-free brightfield images, high noise and background, object tracking, etc. Tools like edge detection or various noise filters help dealing with limitations of image acquisition, while the problems of false positives / negatives through strict thresholds can be overcome through fuzzy logics. Once objects are identified, a few clicks produce a wealth of results like compactness, elliptic fit, border index, position, orientation, relative distances and much more. In this presentation, I will give a short overview how these tools are used for the analysis of real data produced in the facility, where we have processed up to 30,000 images in one experiment. And please note that in September we are going to organise a Definiens Developer XD training course (and an Advanced course a bit later, probably in November), so if you have challenging microscopy data you need to analyse, this FILM Club should give you an idea whether this is your solution. Good imaging, Martin http://www3.imperial.ac.uk/imagingfacility/equipment/licence_definiens http://developer.definiens.com/algorithms.html -- ------------------------------------------------------------------------ *Martin Spitaler, PhD* *FILM - Facility for Imaging by Light Microscopy* - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler@imperial.ac.uk <mailto:m.spitaler@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility