Dear users, As you remember, we have been awarded a Wellcome Trust grant to purchase a high-content microscope. We are now in the process of organizing demos and for that we need you (and your samples)! Is high-content for you? If you are imaging multi-well plates to follow gene-expression, or trying to analyse multiple cell populations after drug treatment, doing some small-scale RNAi screens, imaging nano-particle uptake, following spheroids or organoids or even tracking several fish over time: this is high-content imaging, and your samples will be invaluable to test the flexibility and functionality of the setups as well as the analysis. Please send us an email if you wish to provide samples, with a short description of your experiment, dye/fluorescent protein type, support type (multi-well plate, luer cell, slide, ...), environmental conditions, flow, expected outcome, or just come to chat with us. The more varied the sample types, the more thoroughly we can test both the acquisition setup and the analysis packages. So far, we are going to test several microscopes/platforms: - ScanR from Olympus (2-6th March, SAF): microscope setup - Operetta from Perkin Elmer (28-29th April, SAF): confocal modality, box setup - ImageXpress Ultra from Molecular Devices (demo dates TBC, SAF): box setup - Nikon WF/confocal (demo dates TBC): dual modality on microscope setup Let us know, preferentially by the end of this week! :) Debbi & Steve Debora Keller, PhD FILM - Facility for Imaging by Light Microscopy - Facility Manager - Super-Resolution Specialist - Sir Alexander Fleming Building, desk 408 Imperial College London / South Kensington Exhibition Road London SW7 2AZ, UK Phone: +44 (0)207 594 2023 Mobile: + 44(0) 7760 256 889 E-mail: d.keller@imperial.ac.uk<mailto:d.keller@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility