Dear microscopists, this year we are are having a microscopy day again, a whole day of lectures, presentations and demonstrations about microscopy: MICROSCOPY DAY, MONDAY 20 JUNE 2011, 9.45-17.00, SAFB-G34 Following up and expending on the successful event two years ago, we are offering information from the very basics to the highly sophisticated, from fluorophores to image analysis. The details are still being finalised, if you are interested in a particular topic please let me know. PRELIMINARY PROGRAMME: 9.45- 9.50 Introduction 9.50-10.30 Basics of microscopy: properties of light, diffraction limit, resolution, contrast methods, epifluorescence, confocal, ... 10.30-11.15 Principles of fluorescence: fluorophores, photo-bleaching, photo-switching, phototoxicity, ... 11.15-11.30 coffee 11.30-12.15 Special techniques I: two photon microscopy, second harmonic generation, in vivo imaging, Flim, Fret, ... 12.15-13.00 Special techniques II: high-resolution microscopy (TIRF, PALM, STORM), ... 13.00-14.30 lunch break and demonstrations: - TIRF, PALM, STORM - 2P, SHG, in vivo, Flim - tour of EM facility - OMERO image database, XperimentR - image analysis tools 14.30-15.15 Transmission electron microscopy 15.15-16.00 Understanding and handling images: data formats, pixel and metadata, visualisation, OMERO database, analysis workflow, ... 16.00-16.15 coffee 16.15-17.00 Tools for image analysis: filtering, edge detection, segmentation, feature extraction, morphoplogy, ... For more details and updates, see FILM website / NEWS (http://www3.imperial.ac.uk/portal/page/portallive/imagingfacility/news). Everybody is welcome. see you there, and good imaging, Martin ######################################### Martin Spitaler, PhD FILM - Facility for Imaging by Light Microscopy - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler@imperial.ac.uk Website: http://imperial.ac.uk/imagingfacility