...can anyone help Parul? Typical nuclear dyes (e.g. PE) don't seem to go into bacteria easily, even when they are dead, Martin -------- Original Message -------- Subject: Live/dead E.coli staining Date: Tue, 22 Mar 2011 16:46:43 +0000 From: Mehta, Parul <p.mehta10@imperial.ac.uk> To: Spitaler, Martin <m.spitaler@imperial.ac.uk>, film-users <film-users@imperial.ac.uk> Hi, I wanted to know if anyone of the FILM users have done live/dead E.coli staining to differentiate between live E.coli cells to dead cells. Many thanks and kind regards, Parul Mehta PhD student Martin Buck's lab, 4th floor SAF Division of Biology Faculty of Life Sciences Imperial College London Sir Alexander Fleming Building South Kensington Campus London SW7 2AZ