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Cell cultures, by definition, inhabit
the unsteady ground between tight,
reproducible experimental control and
the ability to simulate the complex
and dynamic environments found in
vivo. This webcast reveals how
pioneering microfluidic technology
enables the precise manipulation of
physiologically relevant
micro-environments, helping
researchers connect
cellular mechanisms with phenotypes
and disease states.
Microfluidic cell culture chambers
allow crucial parameters such as media
flow, temperature and gas environment
to be automated. This enables
researchers
to recreate the mass transport
environment of tissues, elicit cell
responses
to dynamic solution changes and enable
long-term perfusion culture – a
real-time, live cell analysis with
unprecedented control.
The first speaker, Dr. Andrew Ball,
will review a specific workflow
involving
microfluidic chambers used with a
fluorescent microscope and image
analysis
software, to provide insights for live
cell dynamic applications such as
host-pathogen interactions, cancer
cell autophagy, and cell migration in
a standardized format.
Then, Dr. KC Huang, will discuss how
specific microfluidic techniques have
allowed him to study damped
oscillations of bacterial growth in
response
to osmotic shock. Dr. Huang will
describe the advantages of real-time,
live cell analysis in a microfluidic
context.
In this webcast you learn:
• The advantages and challenges of
using microfludic cell culture tools
in cell biology
• Techniques that can be used to
develop and quantify in vitro
cell models for predictive cell
analysis. |
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