Dear All,

 

Please find below details of the Photonics Seminar that will take place on Tuesday 4^th June in Blackett 630 from 1pm.

 

Computational and Optical Approaches to Multidimensional Live Cell Imaging

Kevin W Eliceiri, University of Wisconsin

 

Light microscopy is providing key insights into cellular dynamics of normal and disease processes, a task being greatly facilitated by technical developments in optical probes and instrumentation. Fluorescent protein reporters allow virtually any protein to be labelled and thereby visualized in a cell, tissue or organism. Along with these developments in fluorescence probe technology, new optical techniques such as Fluorescence Resonance Energy Transfer (FRET), Multiphoton Laser-Scanning Microscopy (MPLSM), Second Harmonic Generation (SHG) imaging, Selective Plane Illumination Microscopy (SPIM) and Fluorescence Lifetime Imaging (FLIM), are revealing how individual cellular components are assembled into cytoplasmic machinery, and how this machinery functions. Our central aim is to develop hardware and software strategies for extracting all the data possible from the weak fluorescence signals that are typically obtained when observing living specimens. This involves the development and application of hardware and software for acquisition, analysis and visualization including feature extraction, exponential curve fitting, spectral unmixing and 3D visualization approaches. Much of this work is under the umbrella of our ImageJ2 software development to offer practical tools for developers and end user alike for the analysis of microscopy image data.

 

Kind regards,

Marcia

 

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Marcia Salviato                                                    Direct Line: +44 (0) 207 594 7862

Deputy Group Administrator     |     Optics Section     |    Department of Physics

Faculty of Natural Sciences                                                 Imperial College London

Room 606, Blackett Laboratory   |   Prince Consort Road   |   London SW7 2BW

 

Physics website: www.imperial.ac.uk/physics

Optics website: www.imperial.ac.uk/research/optics

Photonics website: http://www.imperial.ac.uk/research/photonics

Quantum Optics & Laser Science website: http://www.imperial.ac.uk/research/qols