Dear microscopists, the academic year is approaching its end, and as usual we conclude our FILM Club series with the FILM Microscopy Day 2014: *FILM MICROSCOPY DAY 2014, Wednesday 25 June, SAFB-G34 (South Kensington Campus), 10.00-17.00* Again a whole day full of exciting talks and presentations about microscopy, better and bigger than ever - this time with not one, but two plenary lectures, one by internal microscopist Cristina Lo Celso, the other by Phillip Kukura from Oxford. And we hope many of you will add to the exciting programme with your own 3-min presenation of the microscopy you are doing in your lab: The Microscopy Matchmaker Session has always been a highlight of the day, and the more presentations we get, the more fun it will be, and the better your chances of getting to know other people's work and starting new collaborations. See also the full invitation at <https://workspace.imperial.ac.uk/imagingfacility/Public/FILM%20Microscopy%20Day%202014%20invitation.pdf> So keep the day free, good imaging, Martin http://www3.imperial.ac.uk/imagingfacility/news#md _*Agenda *_ *_Morning Session_*** *10.00-10.15Welcome and latest FILM News (Tony Magee) *New microscopes, new equipment, new staff *10.15-11.15 Basics of microscopy (Martin Spitaler, FILM) *Image formation, fluorescence, visualisation, quantitation */11.15-11.35 coffee & tea break/*** *11.35-12.15 Sample preparation for live and fixed samples (Stephen Rothery, FILM) *Fixatives, strategies, viability, autofluorescence ** *_Microscopy Match-Maker_*** *12.15-13.00 Microscopy Match-Maker *Research performed in FILM, presented by users through 3 min snapshot presentations */13.00-14.00 Sandwich lunch /*/with opportunity to see the new super-resolution microscope / *_Afternoon Session_*** *14.00-14.40 Super-resolution microscopy (Debora Keller, FILM) *PALM, STORM, SIM, resolution limits, artefacts */14.40-15.00 coffee & tea break/*** *_PLENARY SESSION _*** *15.00-15.55Cristina Lo Celso, Imperial College: /"Intra-vital imaging in stem cell biology"/* *15.55-16.50 Philipp Kukura, Oxford/: "Molecular Imaging"/* *16.50-17.00 Tony Magee: Round-up * ** *FILM Microscopy Day 2014: Plenary session*** *Cristina Lo Celso, Imperial College*: */"Intra-vital imaging in stem cell biology"/* After a PhD from University College London in the field of epidermal stem cell biology, Dr Lo Celso worked as a postdoc at Harvard University developing intra-vital microscopy of the haematopoietic stem cell niche. In 2009 she was appointed assistant professor at Imperial College London, where she has established a research group focussing on understanding the cellular mechanisms regulating haematopoietic stem cell (HSC) function and leukaemia development and . In close collaboration with FILM, her group has developed tools to study HSCs in their native bone marrow environment. She will present an interdisciplinary approach combining intra-vital microscopy techniques, computational image analysis, molecular profiling and mathematical modelling and the group's most recent observations of the dynamic interactions between HSCs and the bone marrow microenvironment. *Philipp Kukura, Oxford/: "Molecular Imaging" /* The primary goal of optical microscopy is to visualise microscopic structure and dynamics. Developments over the past decades have enabled routine studies down to the single molecule level and structural observations far beyond the diffraction limit through the use of fluorescence as a contrast mechanism. Despite its many advantages, one of the fundamental limitations of fluorescence detection is the frequency with which photons can be emitted and thus detected. As a consequence, imaging speeds remain limited to few to tens of frames per resulting in a considerable gap between the speed at which dynamics can be recorded and the speed of motion on the nanoscale. I will describe an alternative approach to optical microscopy that relies on the ultra-efficient detection of light scattering called interferometric scattering microscopy (iSCAT). I will show that iSCAT can follow the motion of nanoscopic labels with nm precision down to the microsecond regime, the relevant timescale for a majority of dynamics on the nanoscale. Thereby, we are able to address a surprising variety of fundamental questions ranging from the mechanism underlying processive molecular motion to the existence and formation of lipid rafts in membranes. Finally, I will show that iSCAT can detect, image and track the motion of single, proteins using light scattering without the need for any labels. _* *_ -- ------------------------------------------------------------------------ *Martin Spitaler, PhD* *FILM - Facility for Imaging by Light Microscopy* - Facility Manager - Sir Alexander Fleming Building 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK
